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Addgene/pAAV.CMV.PI.EGFP.WPRE.bGH/1unit/105530-AAV8
免费咨询热线
4000-520-616
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Plasmid | 105530 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | Add to Cart | |
| AAV1 | 105530-AAV1 | Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information | Add to Cart | |||
| AAV2 | 105530-AAV2 | Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information | Add to Cart | |||
| AAV5 | 105530-AAV5 | Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information | Add to Cart | |||
| AAV8 | 105530-AAV8 | Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information | Add to Cart | |||
| AAV9 | 105530-AAV9 | Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information | Add to Cart | |||
| AAV rh10 (similar to AAV10) | 105530-rh10 | Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information | Add to Cart | |||
This material is available to academics and nonprofits only.
Backbone
- Vector backbonepAAV(Search Vector Database)
- Vector typeMammalian Expression, AAV
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)NEB Stable
- Copy numberUnknown
Gene/Insert
- Gene/Insert nameEGFP
- PromoterCMV
Cloning Information
- Cloning methodUnknown
- 5′ sequencing primerCMV-F (Common Sequencing Primers)
Resource Information
- Supplemental Documents
- pAAV.CMV.PI.EGFP.WPRE.bGH (p0101).gb
- Terms and Licenses
- UBMTA
- Ancillary Agreement for Plasmids Containing FP Materials
- Industry Terms
- Not Available to Industry
- Article Citing this Plasmid
- 1 Reference
Depositor Comments
Penn Vector Core number p0101
Information for AAV1 (Catalog # 105530-AAV1)(Back to top)
Purpose
Ready-to-use AAV1 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA.
CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 1×10¹³ vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV1
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneEGFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
Quality Control:
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Information for AAV2 (Catalog # 105530-AAV2)(Back to top)
Purpose
Ready-to-use AAV2 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA.
CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 7×10¹² vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV2 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV2
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneEGFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
Quality Control:
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Information for AAV5 (Catalog # 105530-AAV5)(Back to top)
Purpose
Ready-to-use AAV5 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA.
CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 7×10¹² vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
- BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
- SerotypeAAV5
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneEGFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
Quality Control:
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Information for AAV8 (Catalog # 105530-AAV8)(Back to top)
Purpose
Ready-to-use AAV8 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA.
CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 1×10¹³ vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV8 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV8
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneEGFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
Quality Control:
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Information for AAV9 (Catalog # 105530-AAV9)(Back to top)
Purpose
Ready-to-use AAV9 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA.
CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 1×10¹³ vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV9 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV9
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneEGFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
Quality Control:
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Information for AAV rh10 (similar to AAV10) (Catalog # 105530-rh10)(Back to top)
Purpose
Ready-to-use AAV rh10 (similar to AAV10) particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA.
CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 1×10¹³ vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAVrh10 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAVrh10
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneEGFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
Quality Control:
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
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常见问题
蚂蚁淘所售产品均为正品吗？
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实验台生物安全柜制冰机霉菌培养箱厌氧培养箱光照培养箱电热恒温...123
2021-07-26
SKP-02数显式电热恒温培养箱，是恒温培养箱系列中的一款。SKP-02数显式电热恒温培养箱不仅可供大专院校、生物、农业、科研等部门作储藏菌种、生物培养，进行科研使用，SKP-02数显式电热恒温培养箱也可做细菌培养、发酵及恒温试验用。
SKP-02数显式电热恒温培养箱的主要技术参数：
型号：SKP-02
工作室规格:250*250*300（功率0.1KW）、300*300*360（功率0.2KW）、420*420*500（功率0.4KW）、500*500*600（功率0.6KW）、600*600*710 （功率0.7KW）
温控范围：室温+5℃～65℃
SKP-02数显式电热恒温培养箱的特点：
1、采用微电脑控制，具有PID自协调功能。
2、独具超温保护、定时功能。
3、优质冷扎板内室，大视角双层观察窗。
4、外壳喷塑。
SKP-02数显式电热恒温培养箱适用于大专院校、生物、农业、科研等部门作储藏菌种、生物培养，SKP-02数显式电热恒温培养箱是进行科研的必须设备。SKP-02数显式电热恒温培养箱智能化的微电脑控制，精细准确。
SKP-02数显式电热恒温培养箱的主要技术参数：
型号：SKP-02
工作室规格:250*250*300（功率0.1KW）、300*300*360（功率0.2KW）、420*420*500（功率0.4KW）、500*500*600（功率0.6KW）、600*600*710 （功率0.7KW）
温控范围：室温+5℃～65℃
SKP-02数显式电热恒温培养箱的特点：
1、采用微电脑控制，具有PID自协调功能。
2、独具超温保护、定时功能。
3、优质冷扎板内室，大视角双层观察窗。
4、外壳喷塑。
SKP-02数显式电热恒温培养箱适用于大专院校、生物、农业、科研等部门作储藏菌种、生物培养，SKP-02数显式电热恒温培养箱是进行科研的必须设备。SKP-02数显式电热恒温培养箱智能化的微电脑控制，精细准确。
常用的抗生素有哪些123
许你余下年华2017-11-07
电热恒温培养箱、隔水式恒温培养箱、生化培养箱、霉菌培养箱、光照培养箱、人工气候箱、二氧化碳培养箱
皮氏培养皿_皮氏培养皿【价格,厂家,图片,批发,采购】123
2017-11-07
生化培养箱,光照培养箱,人工气候箱有区别吗?我们实验室需要一台做细菌培养的仪器,该怎么选择，请使用过的同行推荐下吧
烘箱与培养箱之间有什么区别123
小伙2yZ32017-11-07
干燥箱用于干燥物品或者干热灭菌。其恒温调节范围，（50~200）℃（或（50~250））℃、（50~300）℃；培养箱用于培养细菌等。其恒温调节范围：自高于室温起至60℃。
干燥箱可分为普通式和鼓风式两种。后者在箱内装有一个风机，用以加快热空气的对流，使箱内温度均匀。同时使箱内物品蒸发的水蒸气加速散发到箱外的空气中，以提高干燥效率。
培养箱主要有直热式和隔水式两种：直热式用电热丝直接加热。隔水式培养箱的恒温室被水箱包围，通电后，加热的是水，利用热水的温度使箱内恒温，故为间接加热。这种培养箱温度的上升或下降较为缓慢，更适合于细菌培养箱。
干燥箱可分为普通式和鼓风式两种。后者在箱内装有一个风机，用以加快热空气的对流，使箱内温度均匀。同时使箱内物品蒸发的水蒸气加速散发到箱外的空气中，以提高干燥效率。
培养箱主要有直热式和隔水式两种：直热式用电热丝直接加热。隔水式培养箱的恒温室被水箱包围，通电后，加热的是水，利用热水的温度使箱内恒温，故为间接加热。这种培养箱温度的上升或下降较为缓慢，更适合于细菌培养箱。
双抗处理细胞在4°C冰箱还是37°C培养箱？ 间充质干细胞专区 干细胞...123
lily1322021-08-04
生物培养箱 123
茜茜000112017-09-29
培养箱是全新无氟设计，国际品牌压缩机和循环风机，超大液晶屏，操作方便，效率高、能耗低。不仅促进节能，而且使用寿命长，可将噪声降至更低限度，与传统低温设备相比，降温时间减少40%以上。向左转|向右转
培养箱位置不够,叠起来放培养皿可以吗? 食品理化检测 食品论坛...123
懒洋洋Xrw2021-08-09
只要提供给它一定的培养温度就可以，
叠多少层没影响。
按ISO标准，每叠不超过6个为宜。
叠多少层没影响。
按ISO标准，每叠不超过6个为宜。
【求助】培养箱长霉菌怎么办? 细胞技术讨论版论坛123
小颜066rUz2021-08-05
霉菌对于酒精、紫外线都有很强的抵抗能力，尤其是其孢子更难消灭，但是对于热很敏感，一般真菌60℃1小时菌丝与孢子可被杀死，所以建议细胞培养箱的金属架子取出喷上酒精火烧一遍，烧时要架空，
生化培养箱和霉菌培养箱如果选择？123
微风引弱火2021-08-01
霉菌培养箱是生化培养箱的一种，买什么培养箱根据需要培养的生物内容、要求来选定，若实验室要求培养厌氧菌，那就需要买二氧化碳培养箱了。
美国Thermo HERAcell 150i二氧化碳培养箱 二氧化碳培养箱|CO2培养...123
wwj71682021-07-20
过完年，再开培养箱参数不稳定，两个箱子，co2数值一直在闪～我要养细胞，参数设置的是37℃，5%CO2,请问各位大神有知道咋回事的～还有面板上各种符号啥意思？图中标记的都是在闪的参数
细胞培养箱中为什么要放水盘 123
二货乚2021-08-10
如果不放,培养箱内部的湿度会较低,培养基中的水分很容易挥发,使液体培养基浓缩；固体培养基变干.
细胞培养箱出现rh pan 是什么意思 123
莫颜853062017-11-07
应该指的是，培养箱里的水箱水位低了，需要从注水孔中补充足够的水分。
具体可以查看一下说明书。
具体可以查看一下说明书。
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