- Description
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Description
Details
Description:Rabbit anti-Mouse potassium channel-interacting protein4 (Kchip4) polyclonal antibody
Purification: Protein G affinity purified
Product Type:Primary antibody
Target Protein:Kchip4
Immunogen:A synthetic peptide (KTSSPAIQNSV) derived from the amino sequence acid residue 45-57 of mouse Kchip4, conjugated with KLH for immunization
Specificity:This antibody is reactive to the 29kDa mouse Kchip4 in mouse brain tissue extract on Western blot.
Species Reacitvity:Mouse and other species with consensus Kchip4 sequence (KTSSPAIQNSV)
Source:Rabbit
Formulation:Lyophilized in pH 7.4, 0.01M PBS
Reconstitution:Double distilled water is recommended to adjust the final concentration to 1.00 mg/mL.
Storage:Store at -20oC
Research Area:Life science research, stem cell factor signalling, cancer research
Background:
KChIP4 is a member of the potassium channel interacting protein family. It possesses calcium binding domains like other members of the KChIP family. KChIP4 is cloned accidentally with the yeast two- hybrid method using Presenilin as bait. The newly discovered protein does not affect the function of Presenilin, but instead, it will reconstitute the feature of A-type Kv channel after co-expression with Kv4.2, indicating that it is a potassium channel interacting protein.
Application:
ELISA:Dilute the antibody approx. 1:300,000 for detection of Kchip4 peptide (KTSSPAIQNSV) coated on ELISA plate.
Western blot:Dilute approx. 1:4,000 for using in Western blotting to detect the 29kDa Kchip4 in mouse brain extract.
Fig.1 Western blot analysis of extracts from mouse brain using anti-kchip4 rabbit whole antiserum.
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
| Product Specificity | pAb Rabbit anti-Mouse Kchip4 |
|---|---|
| Application | EIA, WB |
| Size | 0.1 mg |
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缺点也很多。激光的功率很小，对荧光信号的灵敏度和分辨率都很差。只有很强的信号才适合于在这个机器上使用。如果经常要改变染色的步骤，使用不同的样本，或者抗原的量不确定，不建议用这个机器。
在流式细胞仪中，用PI单染法区分死细胞和活细胞，怎么看数据结果？
不过还要考虑很多因素：标记的方法会不会影响细胞下面的培养细胞是否能够耐受长时间接触房间中的氧气和二氧化碳的浓度机器的无菌情况
（溶血）：红细胞，血红蛋白分解，红细胞溶解逃逸所述，称为溶血。通过各种物理和化学因素和毒素。在体外，如低渗溶液中，强烈的机械振荡，突然冷冻（-20℃-25℃）或突然化冻，过酸或过碱，以及乙醇，乙醚，皂碱，胆碱盐可引起溶血。人血浆的等渗溶液为0.9％NaCl溶液，红细胞在低于0.45％的NaCl溶液中，由于水的渗透，肿胀和红细胞破裂，血红蛋白逸出。在体内，溶血溶血性细菌侵入或某些毒素的抗原 - 抗体反应（如输入配血不合的血液），各种机械性损伤，红细胞内在（膜，酶）引起某些药物的缺陷。溶血性细菌，如某些溶血性链球菌和产气荚膜梭菌可导致败血症。的红血细胞和某些溶血性毒液含酶卵磷脂，卵磷脂血浆或红细胞成溶血卵磷脂，使红细胞膜分解疟原虫破坏。
由于研究的就没有限制了。
举几个例子：
96孔盘单个细胞分选，目标细胞只要96个。如果阳性率是50%，那几百个细胞就可以了，如果阳性率不到1%，那几万个细胞也不一定能选出来。
或者，阳性率有20-30%，但是下游实验需要几百万个细胞，那你需要几千万的细胞才可以满足。
类似于天文学上观察星星。光线从星球的背后照射过来。FSC看的是激光照射细胞后，从四周散发的光线，越大的细胞光晕越大。信号也越强
而一些老式 的机器，由于装备的激光数量和检测器都很有限，比如BD calibur这款机器。最高配置也就是蓝色（488nm）和红色（647nm）两种激光。检测4种颜色（通道）。所以为方便起见，就命名为FL1,FL2, FL3和FL4。前三种由于蓝色激光，FL4用于检测红色激光所激发的荧光。其中FL1是检测绿色荧光的（比如FITC，荧光素或者绿色荧光蛋白），而FL2是检测橙色荧光的（比如PI染色）。
过表达质粒转染细胞，以空载质粒做对照，48h后检测对凋亡的影响。请问定象限的时候是用空载质粒的对照组，还是需要用未转染的正常细胞？
