Addgene/pAAV-CAG-GFP/1unit/37825-AAV9.T_蚂蚁淘，【正品极速】生物医学科研用品轻松购|ebiomall 蚂蚁淘商城

商品信息

联系客服

郑重提醒：

无质量问题不接受退换货，下单前请仔细核对信息。

下单后请及时联系客服核对商品价格，订单生效后再付款。

Addgene/pAAV-CAG-GFP/1unit/37825-AAV9.T

品牌 /

Addgene

货号 /

37825-AAV9.T

官网地址

美元价：

登录查看

(友情提示：该价格仅为参考，欢迎联系客服询价！)

数量：

免费咨询热线

4000-520-616

商品介绍售后保障相关文章商品咨询

联系客服

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	37825	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart
AAV1	37825-AAV1	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV1 trial size	37825-AAV1.T	Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$130	Add to Cart
AAV2	37825-AAV2	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV2 trial size	37825-AAV2.T	Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$130	Add to Cart
AAV5	37825-AAV5	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV5 trial size	37825-AAV5.T	Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$130	Add to Cart
AAV8	37825-AAV8	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV8 trial size	37825-AAV8.T	Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$130	Add to Cart
AAV9	37825-AAV9	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV9 trial size	37825-AAV9.T	Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$130	Add to Cart
AAV Retrograde	37825-AAVrg	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV Retrograde trial size	37825-AAVrg.T	Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$130	Add to Cart
AAV PHP.eB	37825-PHPeB	Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information		$380	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
AAV with CAG promoter
(Search Vector Database)
Backbone manufacturer
Scott Sternson
Backbone sizew/o insert(bp)4722
Vector type
Mammalian Expression, AAV ; Adeno-associated virus

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin
Growth Temperature
30°C
Growth Strain(s)
NEB Stable
Growth instructions
Use recombinase-free E. coli (Stbl3, XL-10, Sure2 and DH5a et.al), grow at 30C
Copy number
Low Copy

Gene/Insert

Gene/Insert name
GFP
Alt name
Green fluorescent protein
Species
A. victoria
Insert Size (bp)
720
Mutation
N/A
PromoterCAG

Cloning Information

Cloning methodRestriction Enzyme
5′ cloning siteBamHI(not destroyed)
3′ cloning siteEcoRI(not destroyed)
5′ sequencing primeraaagagacagcaaccagg
3′ sequencing primercggcttctggcgtgtgac

Resource Information

Supplemental Documents
- pAAV-CAG-GFP.gb
Terms and Licenses
- UBMTA
- Ancillary Agreement for Plasmids Containing FP Materials
Industry Terms
- Not Available to Industry
Articles Citing this Plasmid
- 12 References

Depositor Comments

PLEASE CONTACT ED BOYDEN ([email protected]) FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.

Information for AAV1 (Catalog # 37825-AAV1)(Back to top)

Purpose

Ready-to-use AAV1 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV1
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV1 trial size (Catalog # 37825-AAV1.T)(Back to top)

Purpose

Ready-to-use AAV1 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume20 µL
Titer≥ 7×10¹² vg/mL
Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV1
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV2 (Catalog # 37825-AAV2)(Back to top)

Purpose

Ready-to-use AAV2 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV2 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV2
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV2 trial size (Catalog # 37825-AAV2.T)(Back to top)

Purpose

Ready-to-use AAV2 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume20 µL
Titer≥ 7×10¹² vg/mL
Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV2 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV2
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV5 (Catalog # 37825-AAV5)(Back to top)

Purpose

Ready-to-use AAV5 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV5
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV5 trial size (Catalog # 37825-AAV5.T)(Back to top)

Purpose

Ready-to-use AAV5 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume20 µL
Titer≥ 7×10¹² vg/mL
Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV5
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV8 (Catalog # 37825-AAV8)(Back to top)

Purpose

Ready-to-use AAV8 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV8 cap gene
BufferPBS + 0.001% Pluronic F-68
SerotypeAAV8
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV8 trial size (Catalog # 37825-AAV8.T)(Back to top)

Purpose

Ready-to-use AAV8 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume20 µL
Titer≥ 7×10¹² vg/mL
Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV8 cap gene
BufferPBS + 0.001% Pluronic F-68
SerotypeAAV8
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV9 (Catalog # 37825-AAV9)(Back to top)

Purpose

Ready-to-use AAV9 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV9 cap gene
BufferPBS + 0.001% Pluronic F-68
SerotypeAAV9
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV9 trial size (Catalog # 37825-AAV9.T)(Back to top)

Purpose

Ready-to-use AAV9 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume20 µL
Titer≥ 7×10¹² vg/mL
Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV9 cap gene
BufferPBS + 0.001% Pluronic F-68
SerotypeAAV9
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV Retrograde (Catalog # 37825-AAVrg)(Back to top)

Purpose

Ready-to-use AAV Retrograde particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

GFP expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV retrograde cap gene from rAAV2-retro helper (plasmid #81070)
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV retrograde (AAVrg)
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Addgene Comments

Retrograde functionality is dependent on high viral titers. Addgene recommends not diluting your AAV preps prior to use.

Data submitted about 37825-AAVrg by requesting scientist(s):

Data 1: Guinea pig, Brain parenchyma

Information for AAV Retrograde trial size (Catalog # 37825-AAVrg.T)(Back to top)

Purpose

Ready-to-use AAV Retrograde trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

Delivery

Volume20 µL
Titer≥ 7×10¹² vg/mL
Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV retrograde cap gene from rAAV2-retro helper (plasmid #81070)
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV retrograde (AAVrg)
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Addgene Comments

Retrograde functionality is dependent on high viral titers. Addgene recommends not diluting your AAV preps prior to use.

Information for AAV PHP.eB (Catalog # 37825-PHPeB)(Back to top)

Purpose

Ready-to-use AAV PHP.eB particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
TiterEnter the titer here≥ 1×10¹³ vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, PHP.eB cap gene pUCmini-iCAP-PHP.eB (plasmid #103005)
BufferPBS + 0.001% Pluronic F-68
SerotypePHPeB (plasmid #103005)
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Addgene Comments

Citation Information: When using the PHP.eB serotype in future publications, please acknowledge Viviana Gradinaru and cite Chan et al., Nat Neurosci, 20(8):1172-1179. Pubmed.

蚂蚁淘电商平台
ebiomall.com

公司简介

蚂蚁淘（www.ebiomall.cn）是中国大陆目前唯一的生物医疗科研用品B2B跨境交易平台，该平台由多位经验丰富的生物人和IT人负责运营。蚂蚁淘B2B模式是指客户有采购意向后在蚂蚁淘搜索全球供应信息，找到合适的产品后在蚂蚁淘下单，然后蚂蚁淘的海外买手进行跨境采购、运输到中国口岸，最后由蚂蚁淘国内团队报关运输给客户...

蚂蚁淘承诺

正品保证： 全球直采在线追溯蚂蚁淘所有产品都是自运营的，我们已经跟国外多家厂方建立品牌推广合作关系, 获得对方的支持和授权；同时客户可以通过订单详情查看到货物从厂方至客户的所有流程，确保货物的来源；正规报关，提供13%增值税发票。

及时交付： 限时必达畅选无忧蚂蚁淘的运营团队都是有着多年经验的成员，他们熟悉海外采购、仓储物流、报关等环节；同时通过在线的流程监控，蚂蚁淘的进口速度比传统企业提高了50%以上，部分产品甚至能做到7-10天到货，即蚂蚁淘的“时必达”服务。

轻松采购： 在线下单简单省事蚂蚁淘的价格是真实透明的，并且具有很大的价格优势，不需要繁杂的询价比价；报价单与合同可以直接在线生成或打印；就像在京东购物一样，您的鼠标点击几次即完成在蚂蚁淘的采购，订单详情会告诉您所有进程。

售后申请： 耐心讲解优质服务蚂蚁淘提供的产品在使用过程中如因产品质量问题有售后需求时，您可通过我的订单提交您的“申请售后”，蚂蚁淘产品顾问会第一时间为您处理，在售后服务过程中如遇到问题也可致电蚂蚁淘客服热线：4000-520-616。

Domains Category List : P(1057) P 单词类别

2021-07-16

一、流式细胞术的概念流式细胞术(Flow Cytometry, FCM)，是七十年代发展起来的高科学技术,它集计算机技术、激光技术、流体力学、细胞化学、细胞免疫学于一体, 同时具有分析和分选细胞功能。它不仅可测量细胞大小、内部颗粒的性状,还可检测细胞表面和细胞浆抗原、细胞内DNA、RNA含量等,在血液学、免疫学、肿瘤学、药物学、分子生物学等学科广泛应用。二、流式细胞仪的概念流式细胞仪(FCM)是集单克隆抗体、荧光化学、激光、计算机等高... 查看更多>

固醇类祛疤药膏有哪些_有问必答_

2021-09-01

默瑞（上海）生物科技有限公司，Leica显微系统，Merk Millipore实验室纯水超纯水仪，Roche实时定量PCR仪，Partec流式细胞仪，RPA，TwistDx 代理商，Echelon代理商，Jackson代理商,KAPA，enzymatics经销商等,莫纳生物集团企业。查看更多>

大鼠神经肽Y(NPY)酶联免疫分析(ELISA) 试剂盒说明书

2021-07-28

Attune™声波聚焦流式细胞仪是由黑龙江省龙沃科技发展有限公司代理或销售的0品牌的仪器，产品来源于美国应用生物系统公司(ABI)。黑龙江省龙沃科技发展有限公司是中国最权威的Attune™声波聚焦流式细胞仪销售服务商之一，在哈尔滨等地方销售Attune™声波聚焦流式细胞仪已经多年。同时，生物在线为您提供众多企业Attune™声波聚焦流式细胞仪仪器产品及图片，以便挑选到性价比高，合适的Attune™声波聚焦流式细胞仪产品查看更多>

Novatein生物代理品牌:NovateinNovatein网

2021-07-24

Navios流式细胞仪在美国和中国喜获诊断使用批准查看更多>

NAR:蛋白功能层级注释数据库eggNOG5_刘永鑫的博客..._

2019-04-30

中技国际招标有限公司受首都医科大学附属北京佑安医院委托，根据《中华人民共和国政府采购法》等有关规定，现对医用设备购置采购项目进行公开招标，欢迎合格的供应商前来投标。项目名称：医用设备购置采购项目项目编号：PXM2019_193317_000004-JH001-XM001项目联系方式：项目联系人：刘菲项目联系电话：010-63348545 采购单位联系方式：采购单位：首都医科大学附属北京佑安医院查看更多>

蛋白纯化些常见问题总结实验方法

2020-07-05

南京赛泓瑞生物科技有限公司在发布的Ki67细胞增殖检测试剂盒（细胞免疫荧光法/流式细胞仪检测）供应信息，浏览与Ki67细胞增殖检测试剂盒（细胞免疫荧光法/流式细胞仪检测）相关的产品或在搜索更多与Ki67细胞增殖检测试剂盒（细胞免疫荧光法/流式细胞仪检测）相关的内容。查看更多>

体外培养细胞的实验观察方有哪些法

2018-12-26

PI能够插入双链DNA中。它被活细胞排斥但能穿透正在死亡或已经死亡的细胞的细胞膜。Ⅰ、材料1、 PI（e.g.,Cat #537059,Calbiochem,San Diego,CA）2、缓冲体系：1×PBS(Ca2+ and Mg2+ free,e.g.,Cat#9240,Irvine Scientif 查看更多>

蛋白电泳上样缓冲液(5倍稀释)_价格厂家供应商_上海烜雅生物科技...

2018-12-26

Martin 和 Synge在1941年就提出高效相色谱的设想，然而直到六十年代后期，由于各种技术的发展，高效液相色谱才付诸实现。这种色谱技术曾被称为高速液相色谱（HighSpeed Liquid Chromatography），高压液相色谱（High Parss-ure Lipuid Chromatography）查看更多>

用超声波清洗机清洗试管的标准就是主要是直径12cm的试管用超声波...

2021-08-12

德国Partec是全球三大流式细胞仪生产厂家之一，其产品遍布全球180多个国家和地区，是卓越性能和优良品质的代名词。公司成立于1967年，是流式细胞仪的制造鼻祖， PARTEC公司早在1968年生产出第一台商用流式细胞仪，凭借四十余年的研制经验和众多流式分析的专利技术，德国Partec公司在流式细胞分析设备领域，成为世界上著名的、仪器型号、种类全球最齐的流式细胞仪制造商查看更多>

第一届女神班电子竞技宣布JuMayumin离开团队

2019-04-22

自七十年代出现第一代流式细胞仪以来，随着计算机技术、电子制造技术、激光技术及荧光素合成技术的不断发展，现代流式细胞仪已今非昔比，制造工艺、功能、精确度有了质的飞跃。<br>流式细胞仪生产厂商推出各种不同型号的流式细胞仪来满足用户的不同需要。现生产流式细胞的厂商全球至少有六家，各厂商产品又有不同的系列，各具特点。如何从众多的型号中挑选出最适合自己的机型，我们还需从分析应用出发，评估自己的需求来决定什么样的 ... 查看更多>

【xin表】xin表厂家、品牌、图片、热帖

2019-02-26

国义招标股份有限公司受江门市中心医院的委托，对流式细胞仪进行公开招标采购，欢迎符合资格条件的供应商投标。一、采购项目编号：440700-201902-401002-0021 二、采购项目名称：流式细胞仪三、采购项目预算金额（元）：1,600,000 四、采购数量：1台五、采购项目内容及需求：(采购项目技术规格、参数及要求，需要落实的政府采购政策) 设备名称数量采购预算流式细胞仪1台￥1, 查看更多>

超高速的时间戳A50micro Apogee流式细胞仪报价/价格普瑞麦迪(

2020-03-05

香港伯齐科技有限公司在发布的Apogee A50 Micro流式细胞仪供应信息，浏览与Apogee A50 Micro流式细胞仪相关的产品或在搜索更多与Apogee A50 Micro流式细胞仪相关的内容。查看更多>

常见问题

蚂蚁淘所售产品均为正品吗？

蚂蚁淘的创始人兼CEO是钟定松先生，具有十年的从业经验，在业界享有良好的口碑； Ebiomall是跨境直采平台，我们直接从厂家采购，自己的团队负责国际物流和清关，中间没有第三方，蚂蚁淘承诺所售产品仅为正品，假一罚十。

下单后可以修改订单吗？

未确认状态的订单可以修改，打开“订单详情”页面，点击右上角的“修改订单”即可，若已审核确定，则订单无法修改。

商品几天可以发货？

现货产品付款审核后即可发货,大部分期货产品在3周左右即可到货,提供时必达服务的产品订单审核十天内即可发货。

订单如何取消？

如订单处于未确定状态，进入“我的订单"页面，找到要取消的订单，点击“取消订单”按钮。

可以开发票吗？

本网站所售商品都是正规清关，均开具13%正规发票，发票金额含配送费金额，另有说明的除外。

如何联系商家？

蚂蚁淘任何页面都有在线咨询功能，点击“联系客服”、“咨询”或“在线咨询”按钮，均可咨询蚂蚁淘在线客服人员，或拨打4000-520-616，除此之外客户可在联系我们页面找到更多的联系方式。

收到的商品少了/发错了怎么办？

同个订单购买多个商品可能会分为一个以上包裹发出，可能不会同时送达，建议查看订单详情是否是部分发货状态；如未收到，可联系在线客服或者致电4000-520-616。

退换货/维修需要多长时间？

一般情况下，退货处理周期为客户收到产品一个月内（以快递公司显示签收时间为准），包装规格、数量、品种不符，外观毁损、短缺或缺陷，请在收到货24小时内申请退换货；特殊商品以合同条款为准。

商品咨询

BD Accuri® C6 Plus个人型流式细胞仪性能参数,报价/价格,图片...123

机器皮蛋106号2017-11-04

这个机器优点是比较小，使用也方便

缺点也很多。激光的功率很小，对荧光信号的灵敏度和分辨率都很差。只有很强的信号才适合于在这个机器上使用。如果经常要改变染色的步骤，使用不同的样本，或者抗原的量不确定，不建议用这个机器。

刚学流式细胞仪,还不太会分析,求帮助_问答详情_流式细胞仪_计数...123

A雅雅雅2021-08-11

做的是淋巴细胞分离，然后用流式细胞仪检测，出来了结果，但是不太会看，求帮助

BCAA123

sen37992017-10-24

可以的，目前根据机器的型号不同，可以有两种方法。
比较常用的是用计数微球。比如临床上的CD4细胞绝对计数，就是采用这个方法。试管内事先已经有固定数目的计数微球和染色抗体。按要求加入固定体积的抗凝血液，裂解红细胞后上机。记录一千个微球，同时就会得到不同染色区域细胞的数量。这样就可以算出单位体积血液内CD4细胞的绝对数目了。

这样的计数微球也可以另外购买。浓度是固定的。在你已知体积的细胞悬液中加入一定体积的微球后上机，记录一千个微球的数据，细胞的计数也会同时记录。根据微球的浓度就可以推算出细胞的浓度，从而得出绝对计数了

另外一个方法是有些流式细胞仪是使用微泵加样，而不是连续吸取样本，这样样本的体积是已知的，记录一次就是所有样本的数量，可以算出细胞浓度

【求助/交流】细胞死亡率检测分子生物综合其他 ...123

洛荨XJ2021-08-15

流式细胞仪测细胞死亡率，是直接染色？还是先固定后染色？求各位大神指导一下，非常感谢

人外周血流式细胞仪检测指标正常参考值123

2017-11-04

这个要看你是怎么看的了。流式细胞仪检测的都是相对值和百分比。CD3是T细胞的表面标记，一般认为CD3+的淋巴细胞就是T细胞。
正常人T细胞占到淋巴细胞的60-90%。T细胞一般有分为CD4+ 和CD8+这两种，两者的比例正常范围是0.9-6.

流式细胞仪所检测的信号有哪些？这些信号所代表的意义是什么？ | 答...123

xmsshx2021-07-29

1、FL1-height 代表FL-1这个信号通道每个信号曲线的高度（代表信号强度）。
2、流式细胞仪（Flow cytometry ）是对细胞进行自动分析和分选的装置。它可以快速测量、存贮、显示悬浮在液体中的分散细胞的一系列重要的生物物理、生物化学方面的特征参量，并可以根据预选的参量范围把指定的细胞亚群从中分选出来。多数流式细胞计是一种零分辨率的仪器，它只能测量一个细胞的诸如总核酸量，总蛋白量等指标，而不能鉴别和测出某一特定部位的核酸或蛋白的多少。也就是说，它的细节分辨率为零。

超声波对你的身体健康有什么影响?123

有梦青年112021-08-08

这个和你要配置的激光数量，功率，有没有气溶胶控制系统都有关系，范围很大啊。还和你单位和BD的合同是否有折扣有关。

我有过两台，一台配有三色激光，当时的报价将近40万美元，最近买的另外一台，两个激光，30万美元。

流式细胞仪相关问答,流式细胞仪知道,流式细胞仪疑问,流式细胞...123

小小阿狸梦2021-07-28

流失细胞仪检测细胞数量，可能会出现红色的结果吗？

做蛋白修饰的公司,嚷的另一个读音是什么组词组词网123

小可231sxd唵2021-07-25

现代的流式细胞仪都可以同时测量10多个甚至更多的颜色。已经不再使用FL1，FL2这样的名称了。

而一些老式的机器，由于装备的激光数量和检测器都很有限，比如BD calibur这款机器。最高配置也就是蓝色（488nm）和红色（647nm）两种激光。检测4种颜色（通道）。所以为方便起见，就命名为FL1,FL2, FL3和FL4。前三种由于蓝色激光，FL4用于检测红色激光所激发的荧光。其中FL1是检测绿色荧光的（比如FITC，荧光素或者绿色荧光蛋白），而FL2是检测橙色荧光的（比如PI染色）。

bd流式细胞仪常用试剂与耗材 123

晚晚_WddG22017-10-23

耗材：流式的试管细胞滤网加样的吸管枪头试剂：
染色的各种染料，标记好的荧光抗体染色所需要的缓冲液，封闭液，PBS最终由于上样的缓冲液

帮忙看下这张流式细胞仪得到的图是什么意思胚胎干细胞专区干细胞...123

总在你在的地方2021-07-23

不明白每幅图的纵坐标和横坐标的意思以及各字母的含义，多谢啦，很急，求专业人士点评，或者有相关文献推荐参考的也可以

【深度】政策和需求双重利好眼科显微镜进口替代加速123

2021-08-09

本人用流式细胞仪检测了肿瘤细胞的用药后的凋亡情况，四个象限的意义分别是什么，谢谢！