核酸提取试剂盒价格今日最新核酸提取试剂盒价格行情走势
来自 : 蚂蚁淘
- Collectblood(75microliters)into1mlPBScontaining5microMEDTA(10microlitersof0.5Mstock)andmiximmediatelytopreventclotting.Keeptubesonice.
- RemoveRBCsfromsamples.Thiscanbeaccomplishedbyseveralmeans.AtTheJacksonLaboratory,RBCsarelysedusingeitherGey"ssolutionorabufferedammoniumchloride(ACK)solution.(Alternatively,Becton-Dickinsonsellsaproductcalled"FACSlysisbuffer"thatisusedafterthestainingprotocoltolyseRBCsandfixthecells.)
- Cellsshouldbewashed2-3xwithFACSbuffer(PBSsupplementedwitheither1%BSAor5%FBSandcontaining0.05%NaN3).SUSPendthepelletfromthefinalwashin50microlitersFACSbuffer(ormoreifmorethanoneanalysisistobedoneonasinglesample-uptothreeseparatestainingreactionscanbesetupfromasinglesample).
- Add50microlitersofcellsuspensionto10microlitersofantibodysolutionandmixgently.Youwillneedtodeterminetheproperconcentrationforeachantibodyused.
- Incubatefor30minutesonice.
- Washcells2-3xwithFACSbufferandsuspendin200-300microlitersFACSbufferforanalysis.
- Forlive/deaddiscrimination,add10microliterspropidiumiodide(PI)solution(stock=10micrograms/ml).Iffixingcellsbeforeanalysis,donotaddPI.
免责声明 本文仅代表作者个人观点，与本网无关。其创作性以及文中陈述文字和内容未经本站证实，对本文以及其中全部或者部分内容、文字的真实性、完整性、及时性本站不做任何保证或承诺，请读者仅作参考，并请自行核实相关内容。
版权声明 未经蚂蚁淘授权不得转载、摘编或利用其他方式使用上述作品。已经经本网授权使用作品的，应该授权范围内使用，并注明“来源：蚂蚁淘”。违反上述声明者，本网将追究其相关法律责任。
