
Product Name | Beta - Amyloid (1 - 42), HumanDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA |
Size | 1 mg |
Catalog # | AS-20276 |
US$ | $295 |
Purity | % Peak Area By HPLC ≥ 95% |
Aß (1-42), a major component of amyloid plaques, accumulates in neurons of Alzheimers disease brains. Biochemical analysis of the amyloid peptides isolated from Alzheimers disease brain indicates that Aß (1-42) is the principal species associated with senile plaque amyloids, while Aß (1-40) is more abundant in cerebrovascular amyloid deposit. Solvent Information | |
Detailed Information | ![]() ![]() |
Storage | -20°C |
References | Ref: Nagele, R. et al. Neurosci. 110, 199 (2002); Garzon-Rodriguez, W. et al. J. Biol. Chem. 272, 21037 (1997). |
Molecular Weight | 4514.1 |
DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA | |
Sequence(Three-Letter Code) | H - Asp - Ala - Glu - Phe - Arg - His - Asp - Ser - Gly - Tyr - Glu - Val - His - His - Gln - Lys - Leu - Val - Phe - Phe - Ala - Glu - Asp - Val - Gly - Ser - Asn - Lys - Gly - Ala - Ile - Ile - Gly - Leu - Met - Val - Gly - Gly - Val - Val - Ile - Ala - OH |
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Combined treatment with a BACE inhibitor and anti-Aβ antibody Gantenerumab enhances amyloid reduction in APPLondon mice. J Neurosci 34, 11621. doi: 10.1523/JNEUROSCI.1405-14.2014.Zhang, QG. et al. (2013). Hypersensitivity of the hippocampal CA3 region to stress-induced neurodegeneration and amyloidogenesis in a rat model of surgical menopause. Brain 136, 1432. doi: 10.1093/brain/awt046.Chen, J. & K. Herrup (2012). Glutamine acts as a neuroprotectant against DNA damage, beta-amyloid and H2O2-induced stress. Plos One doi: 10.1371/journal.pone.0033177.Ohta, H. et al. (2012). Effects of NK-4 in a transgenic mouse model of alzheimer"s disease. Plos One doi:10.1371/journal.pone.0030007.Orlando, RA. et al. (2012). A chemical analog of Curcumin as an improved inhibitor of amyloid Abeta oligomerization. Plos One doi: 10.1371/journal.pone.0031869.Takata, K. et al. (2012). Microglial amyloid-β1-40 phagocytosis dysfunction is caused by high-mobility group box protein-1: Implications for the pathological progression of Alzheimers Disease. Intl J Alz Dis doi:10.1155/2012/685739Davis, RC. et al. (2011). Amyloid beta dimers/trimers potently induce cofilin-actin rods that are inhibited by maintaining cofilin-phosphorylation. Mol Neurodegener 6, 10.Dillen, L. et al. (2011). A screening UHPLCMS/MS method for the analysis of amyloid peptides in cerebrospinal fluid of preclinical species. Bioanalysis 3, 45. doi: 10.4155/bio.10.163.Li, W. et al. (2011). Regulation of matrix metalloproteinase 2 by oligomeric amyloid β protein. Brain Res 10.1016/j.brainres.2011.02.078.Li, Y-P. et al. (2011). Syntheses and characterization of novel oxoisoaporphine derivatives as dual inhibitors for cholinesterases and amyloid beta aggregation. Eur J Med Chem 46, 1472. doi: 10.1016/j.ejmech.2011.02.005.Libeu, CP. et al. (2011). Structural and functional alterations in amyloid-β precursor protein induced by amyloid-β peptides. J Alzheimer"s Dis 25, 547.Pandya, A. & JL. Yakel (2011). Allosteric modulator desformylflustrabromine relieves the inhibition of α2β2 and α4β2 nicotinic acetylcholine receptors by β-Amyloid 142 peptide. J Mol Neurosci doi: 10.1007/s12031-011-9509-3.Qu, J. et al. (2011) S-Nitrosylation activates Cdk5 and contributes to synaptic spine loss induced by β-amyloid peptide. PNAS 108, 14330 (2011). doi: 10.1073/pnas.1105172108.Wu, J. et al. (2011). Lipoxin A4 inhibits the production of proinflammatory cytokines induced by β-amyloid in vitro and in vivo. Biochem Biophys Res Commun 408, 382. doi: 10.1016/j.bbrc.2011.04.013.Yin, F. et al. (2011). Silibinin: A novel inhibitor of Aβ aggregation. Neurochem Int 58, 399. doi: 10.1016/j.neuint.2010.12.017.Spuch, C. et al. (2010). A new tacrine-melatonin hybrid reduces amyloid burden and behavioral deficits in a mouse model of Alzheimer"s Disease. Neurotox Res 17, 421. doi: 10.1007/s12640-009-9121-2. Aksenov, MY. et al. (2010). HIV-1 protein-mediated amyloidogenesis in rat hippocampal cell cultures. Neurosci Lett 475, 174. doi:10.1016/j.neulet.2010.03.073.Van Helmond, Z. et al. (2010). Higher soluble amyloid β concentration in frontal cortex of young adults than in normal elderly or Alzheimer"s Disease. Brain Pathol 20, 787. doi: 10.1111/j.1750-3639.2010.00374.x.Nagano, T. et al. (2010). Prostaglandin E2 reduces amyloid β-induced phagocytosis in cultured rat microglia. Brain Res 1323, 11. doi: 10.1016/j.brainres.2010.01.086.Ravindran, C. et al. (2010). CpG-ODNs induces up-regulated expression of chemokine CCL9 in mouse macrophages and microglia. Cell Immunol 260, 113. doi: 10.1016/j.cellimm.2009.10.001.Ryan, D. et al. (2010). An improved method for generating consistent soluble amyloid-beta oligomer preparations for in vitro neurotoxicity studies. J Neurosci Meth 190, 171. doi: 10.1016/j.jneumeth.2010.05.001.Storace, D. et al. (2010). Elevation of β-amyloid 1-42 autoantibodies in the blood of amnestic patietns with mild cognitive impairment. Arch Neurol 67, 867. doi:10.1001/archneurol.2010.137.Tian, G. et al. (2010). Increased expression of cholesterol 24S-hydroxylase results in disruption of glial glutamate transporter EAAT2 association with lipid rafts: a potential role in Alzheimer"s disease. J Neurochem 113, 978. doi: 10.1111/j.1471-4159.2010.06661.x.Tran, TT. et al. (2010). Chronic psychosocial stress accelerates impairment of long-term memory and late-phase long-term potentiation in an at-risk model of Alzheimer"s disease. Hippocampus doi: 10.1002/hipo.20790.Vargas, T. et al. (2010). Gelsolin restores Aβ-induced alterations in choroid plexus epithelium. J Biomed Biotech 10.1155/2010/805405.Wang, Y. et al. (2010). Two-photon and time-resolved fluorescence conformational studies of aggregation in amyloid peptides. J Phys Chem B 114, 7112. doi: 10.1021/jp101496y.Wirths, O. et al. (2010). Pyroglutamate Abeta pathology in APP/PS1KI mice, sporadic and familial Alzheimers disease cases. J Neural Transmission 117, 85. doi: 10.1007/s00702-009-0314-x.Arriagada, C. et al. (2009). Apoptosis is directly related to intracellular amyloid accumulation in a cell line derived from the cerebral cortex of a trisomy 16 mouse, an animal model of Down syndrome. Neuro Lett 470, 81. doi:10.1016/j.neulet.2009.12.062.Giliberto, L. et al. (2009). Mutant presenilin 1 increases the expression and activity of BACE1. J Biol Chem 284, 9027. doi: 10.1074/jbc.M805685200.Gu, Z. et al. (2009). β-Amyloid impairs AMPA receptor trafficking and function by reducing CA2+/calmodulin-dependent protein kinase II synaptic distribution. J Biol Chem 284, 10639. doi: 10.1074/jbc.M806508200.Guix, FX. et al. (2009). Amyloid-dependent triosphosphate isomerase nitrotyrosination induces glycation and tau fibrillation. Brain 132, 1335. doi: 10.1093/brain/awp023.Li, M. et al. (2009). Amyloid Aβ interaction with receptor for advanced glycation end products up-regulates brain endothelial CCR5 expression and promotes T cells crossing the blood-brain barrier. J Immunol 182, 5778. doi: 10.4049/jimmunol.0803013Zhao, L. et al. (2009). Macrophage-mediated degradation of Aβ-amyloid via an apolipoprotein E isoform-dependent mechanism. J Neurosci 29, 3603. doi: 10.1523/JNEUROSCI.5302-08.2009.Jimenez, S. et al. (2008). Inflammatory response in the hippocampus of PS1M146L/APP751SL mouse model of Alzheimer"s Disease: age-dependent switch in the microglial phenotype from alternative to classic. J Neurosci 28, 11650. doi: 10.1523/JNEUROSCI.3024-08.2008.Gevorkian, G. et al. (2008). Amyloid-β peptide binds to microtubule-associated protein 1B (MAP1B). Neurochem Int 52, 1030. doi:10.1016/j.neuint.2007.10.020.Solorzano-Vargas, RS. et al. (2008). Epitope mapping and neuroprotective properties of a human single chain FV antibody that binds an internal epitope of amyloid-beta 1-42. Mol Immunol 45, 881. doi: doi:10.1016/j.molimm.2007.08.008.Watanabe, T. et al. (2008). Spatial memory impairment without apoptosis induced by the combination of beta-amyloid oligomers and cerebral ischemia is related to decreased acetylcholine release in rats. J Pharmacol Sci 106, 84.Chafekar, SM. et al. (2007). Aβ 1-42 induces mild endoplasmic reticulum stress in an aggregation state-dependent manner. Antioxid Redox Signaling 9, 2245.Nelson, TJ. & DL. Alkon (2007). Protection against beta-amyloid induced apoptosis by peptides interacting with beta-amyloid. J Biol Chem 282, 31238. doi: 10.1074/jbc.M705558200.Bales, KR. et al. (2006). Cholinergic dysfunction in a mouse model of Alzheimer disease is reversed by an anti-Aβ antibody. J Clin Invest 116, 825. doi:10.1172/JCI27120.Guo, J-P. et al. (2006). Aβ and tau form soluble complexes that may promote self aggregation of both into the insoluble forms observed in Alzheimer"s disease. PNAS 103, 1953.Shaked, GM. et al. (2006). Aβ induces cell death by direct interaction with its cognate extracellular domain on APP (APP597-624). FASEB J 20, 1254.doi: 10.1096/fj.05-5032fje.Osada, Y. et al. (2005). CLAC binds to amyloid β peptides through the positively charged amino acid cluster within the collagenous domain 1 and inhibits formation of amyloid fibrils. J Biol Chem 280, 8596. doi: 10.1074/jbc.M413340200.Piccini, A. et al. (2005). β-amyloid is different in normal aging and in Alzheimer disease. J Biol Chem 280, 34186.Seidler, N. & TJ. Squire (2005). Aβ-polyacrolein aggregates: Novel mechanism of plastic formation in senile plaques. Biochem Biophys Res Communications 335, 501.Boyd-Kimball, D. et al. (2004). Role of phenylalanine 20 in Alzheimer"s amyloid β-peptide (1-42)-induced oxidative stress and neurotoxicity. Chem Res Toxicol 17, 1743.Legleiter, J. et al. (2004). Effect of different anti-Aβ antibodies on Aβ fibrillogenesis as assessed by atomic force microscopy. J Mol Biol 335, 997. doi:10.1016/j.jmb.2003.11.019.Bard, F. et al. (2003). Epitope and isotope specificities of antibodies to β-amyloid peptide for protection against Alzheimer"s disease-like neuropathology. PNAS 100, 2023. doi:10.1073/pnas.0436286100.Takata, K. et al. (2003). Role of high mobility group protein-1 (HMG1) in amyloid-β homeostasis. Biochem Biophys Res Commun 301, 699. doi:10.1016/S0006-291X(03)00024-X.Uryu, S. et al. (2003). β-amyloid-specific upregulation of stearoyl coenzyme A desaturase-1 in macrophages. Biochem Biophys Res Commun 303, 302. doi:10.1016/S0006-291X(03)00334-6.Arispe, N. & M. Doh (2002). Plasma membrane cholesterol controls the cytotoxicity of Alzheimer"s disease AβP (1-40) and (1-42) peptides. FASEB J 16, 1526. doi: 10.1096/fj.02-0829com.Kanski, J. et al. (2002). The hydrophobic environment of Met35 of Alzheimer"s Aβ(1-42) is important for the neurotoxic and oxidative properties of the peptides. Neurotox Res 10.1080/10298420290023945.Barger, SW. & AS. Basile (2001). Activation of microglia by secreted amyloid precursor protein evokes release of glutamate by cystine exchange and attenuates synaptic function. J Neurochem 76, 846. doi: 10.1046/j.1471-4159.2001.00075.x.Yao, Z-X. et al. (2001). The Ginkgo biloba extract EGb 761 rescues the PC12 neuronal cells from β-amyloid-induced cel death by inhibiting the formation of β-derived diffusible neurotoxic ligands. Brain Res 889, 181. doi: 10.1016/S0006-8993(00)03131-0.Yatin, SM. et al. (2000). Vitamin E prevents Alzheimer"s amyloid β-peptide (1-42)-induced neuronal protein oxidation and reactive oxygen species production. J Alzheimer"s Dis 2, 123.Yao, Z-X. et al. (1999). Free radicals and lipid peroxidation do not mediate β-amyloid-induced neuronal cell death. Brain Res 847, 203. doi: doi:10.1016/S0006-8993(99)02047-8.Yatin, SM. et al. (1999). Alzheimer"s amyloid β-peptide associated free radicals increase rat embryonic neuronal polyamine uptake and ornithine decarboxylase activity: protective effect of vitamin E. Neurosci Lett 263, 17. doi: 10.1016/S0304-3940(99)00101-9.Bradt, BM. et al. (1998). Complement-dependent proinflammatory properties of the Alzheimer"s disease β-peptide. J Exp Med 188, 431. doi: 10.1084/jem.188.3.431. |
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玻璃电极在初次使用前,必须在蒸馏水中浸泡一昼夜以上。
平时也应浸泡在蒸馏水中以备随时使用。玻璃电极不要与强吸水溶剂接触太久,在强碱溶液中使用应尽快操作,用毕立即用水洗净,玻璃电极球泡膜很薄,不能与玻璃杯及硬物相碰;玻璃膜沾上油污时,应先用酒精,再用四氯化碳或乙醚,最后用酒精浸泡,再用蒸馏水洗净。注意电极内要充满氯化钾溶液,应无气泡,防止断路。应有少许氯化钾结晶存在,以使溶液保持饱和状态,使用时拨去电极上顶端的橡皮塞,从毛细管中流出少量的氯化钾溶液,使测定结果可靠。
另外,pH测定的准确性取决于标准缓冲液的准确性。酸度计用的标准缓冲液,要求有较大的稳定性,较小的温度依赖性。
测定时把复合电极插在被测溶液中,由于被测溶液的酸度(氢离子浓度)不同而产生不同的电动势,将它通过直流放大器放大,最后由读数指示器(电压表)指出被测溶液的pH值。酸度计能在0~14pH值范围内使用。酸度计有台式、便携式、表型式等多种,读数指示器有数字式和指针式两种.
1、校正:先将仪器斜率调节器调节在100%位置,再根据被测溶液的温度,调节温度调节器到该温度值。
2、定位:把复合电极插入仪器,选择一种最接近样品pH值的缓冲溶液,把电极放入这一缓冲溶液里,摇动烧杯,使溶液均匀.待读数稳定后,该读数应是缓冲溶液的pH值,否则就要调节定位调节器。用于分析精度要求较高的测定时,要选择两种缓冲溶液(即被测样品的pH值在该两种缓冲溶液的pH值之间或接近)。待第种缓冲溶液的pH值读数稳定后,该读数应为该缓冲溶液的pH值,否则调节定位调节器。清洗电极,吸干电极球泡表面的余液。把电极放入第二种缓冲溶液中,摇动烧杯使溶液均匀,待读数稳定后,该读数应是第二种缓冲溶液的pH值,否则调节斜率调节器。
3、测量:经过pH标定的仪器,即可用来测定样品的pH值。这时温度调节器、定位调节器、斜率调节器都不能再动,用蒸馏水清洗电极,用滤纸吸干电极球部后。把电极插在盛有被测样品的烧杯内,轻轻摇动烧杯,待读数稳定后,就显示被测样品的pH值。复合电极的主要传感部分是电极的球泡,球泡极薄,千万不能跟硬物接触。测量完毕套上保护帽,帽内放少量补充液(3mol/L的氯化钾溶液),保持电极球泡湿润.仪器采用CMOS集成电路,不用时插入短路插头。检修时电烙铁要有良好的接地,以保护仪器。
对pH值准确度要求高的小伙伴们要记得定期校正pH计哦,新一代ÄKTA纯化系统可以在线校正啦:
网上复制的就不要了,求高手相助。。。。谢谢,
仪器分析是化学学科的一个重要分支,它是以物质的物理和物理化学性质为基础建立起来的一种分析方法。利用较特殊的仪器,对物质进行定性分析,定量分析,形态分析。 仪器分析方法所包括的分析方法很多,目前有数十种之多。每一种分析方法所依据的原理不同,所测量的物理量不同,操作过程及应用情况也不同。
便携式pH计主要用于现场和野外测方式,要求较高的精度和完善的功能。
实验室pH计是一种台式高精度分析仪表,要求精度高、功能全,包括打印输出、数据处理等等。
工业pH计是用于工业流程的连续测量,不仅要有测量显示功能,还要有报警和控制功能,以及安 装、清洗、抗干扰等等问题的考虑。
2.据ph计仪器精度分类:可分为0.2级、0.1级、0.05级、0.01级,数字越小,精度越高。
3.根据元器件类型分类:可分为晶体管式、集成电路式和单片机微电脑式,现在更多的是应用微电脑芯片,大大减少了仪器体积和单机成本;但芯片的开发成本很贵。
4根据读数指示分类:可分为指针式和数字显示式二种。指针式pH计现在已很少使用,但指针式仪表能够显示数据的连续变化过程,因此在滴定分析中还有使用。
5.看PH计有没有附带功能,比方说带标配RS232接口,还有一个很重的是温度补偿是自动
还是手动,自动温度补偿的PH计要比手动温度补偿的PH计要方便些,二者之间的价格你就明白了吧。
进口品牌JENCO的性价比比较高。
重量 :1.3kg
外形尺寸 :300*220*90mm
电源电压 :220v-50hz
校准点 :2点
配套电极 :E-301-C
温度补偿方式 :手动-自动温度补偿功能
温度补偿范围 :0~99.9℃
温度测量范围 :0~60℃
稳定性 :±0.01PH±1个字/3h
输入阻抗 :≥1*10(12)Ω
温度误差 :±0.3℃±1个字
MV误差 :±0.1%FS
PH误差 :±0.01PH±1个字
温度精度 :0.1℃
mv精度 :1mv
ph精度 :0.01PH
温度范围 :0~99.9℃
测量范围 :-1999-1999mv
显示范围 :-2.00~18.00PH
测量范围 :0.00-14.00PH
参考价格:2600
告诉你这些参数你能算不。呵呵 我觉得PH计跟功率没多少关系。一个是物理,一个是电化学

