| EA4rPLA2 inhibitor |
Sample solution is provided at 25 µL, 10mM.
Nature.2017 Jan 19;541(7637):417-420.
Nature.2018 Nov;563(7731):407-411.Nature.2018 Jun 13.Nature.2018 Jun 27.Nature.2018 Mar 29;555(7698):673-677.Nature.2017 Sep 7;549(7670):96-100.Nature.2016 Apr 21;532(7599):398-401.
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- Purity ≥ 95.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
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| Cas No. | 389614-94-2 | SDF | Download SDF |
| Synonyms | N/A | ||
| Chemical Name | 7-chloro-6-[4-(diethylamino)phenyl]-5,8-quinolinedione | ||
| Canonical SMILES | CCN(CC)c1ccc(cc1)C1=C(Cl)C(=O)c2ncccc2C1=O | ||
| Formula | C19H17ClN2O2 | M.Wt | 340.8 |
| Solubility | ≤1mg/ml in DMSO;20mg/ml in dimethyl formamide | Storage | Store at -20°C |
| Physical Appearance | A crystalline solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
Ki: 130 μM
EA4 is a rPLA2 inhibitor.
rPLA2, a calcium-dependent cytosolic phospholipase A2 (cPLA2), was initially isolated and characterized from bovine and human red blood cells. With a molecular mass of 42 kDa, cPLA2 shows biochemical properties similar to cPLA2 Type IV.
In vitro: It was found that EA4 was able to inhibit a Ca(2+) ionophore-induced arachidonic acid release from both human and bovine red blood cells, demonstrating that this enzyme was responsible for the Ca(2+)-dependent arachidonic acid release from mammalian red blood cells [1]. Another study found that in mouse hepatoma Hepa-1c1c7 cells, EA4 could cause a significant induction of the CYP1A1-mediated ethoxyresorufin O-deethylase activity time- and concentration-dependently, and such induction was accompanied by an increase of the Cyp1a1 mRNA transcription. Morevoer, in human cells including MCF-7 (human breast adenocarcinoma cell line), HepG2 (human hepatocarcinoma), and HL-60 (human promyelocytic cell line), the expression of CYP1A1 mRNA could be also induced by EA4 treatment. In addition, CYP1B1 mRNA was increased by EA4 in MCF-7 cells [2].
In vivo: Up to now, there is no animal in vivo data reported.
Clinical trial: So far, no clinical study has been conducted.
References:1. Shin, H.S.,Chin, M.R.,Kim, J.S., et al. Purification and characterization of a cytosolic, 42-kDa and Ca2+-dependent phospholipase A2 from bovine red blood cells. The Journal of Biological Chemisty 277, 21086-21094 (2002).2. Chun, Y.J.,Lee, B.Y.,Yang, S.A., et al. Induction of cytochrome P450 1A1 gene expression by a vitamin K3 analog in mouse hepatoma Hepa-1c1c7 cells. Molecules and Cells 12, 190-196 (2001).
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酶标仪选择重复性好的，线性范围宽的，故障率底的，售后服务跟的上的厂家的仪器就好了。
想请教一下各位大神，为什么我做的Caco-2细胞的药物干预，用酶标仪检测CCK8,5/6孔总是异常，按理说，药物干预后，数据应该是递增或递减的，然而234孔递减，5/6孔却上升了，7、8孔又是递减。请问有人有遇到过这种情况吗？？在此先谢谢各位亲了~
CSY-E96D动物疾病快速诊断仪采用固相酶联免疫吸附ELISA的原理,即酶联免疫法；可定量快速畜牧类疾病诊断如禽流感、猪瘟、猪蓝耳、伪狂犬等疾病，广泛应用于养殖场、屠宰场、肉产品深加工企业、检验检疫单位使用。
各位前辈，MTT法加样后第一天阳性组镜下看细胞全死了，样品组和阴性组镜下状态还可以，加DMSO后样品孔和阴性孔颜色粉红，阳性孔红紫到发黑，酶标仪检测，样品组和阴性组大概0.2-0.4，阳性组却有1.2-1.4，请问，这是怎么回事？细胞接板密度为4*104,100μL。向各位求助了，万分感谢！
测器和微处理器控制系统等组成.
光源灯发出的光线经过滤光片或单色器后,成为一束单色光.该单色光束经过酶标板中的待测标本,被标本吸收掉一部分后,到达光电检测器.光电检测器将投照到
上面的光信号的强弱转变成电信号的大小.此电信号经前置放大、对数放大、模数转换等处理后,送人微处理器进行数据处理和计算,最后通过显示器和打印机输出
测试结果.
请问BAC蛋白浓度测定波长是562，我们这边酶标仪的滤光片波长最接近的是595，这样也可以测吗
酶标仪有单波长和双波长检测功能有时使用者不知在什么情况下使用单或双波长检测。所谓的“单波长”就是使用一种对显色具最大吸收的波长即450 nm或492 nm进行比色测定；而“双波长”则除了用对显色具最大吸收的波长即450 nm或492 nm进行比色测定外，同时用对特异显色不敏感的波长如630 nm进行测定，酶标仪最后打印出来的吸光度则为二者之差。630 nm波长下得到的吸光度是非特异的，来自于板子上诸如指纹、灰尘、脏物等所致的吸收。因此，在ELISA比色测定中，最好使用双波长，且不必设空白孔。
