
- SynonymAXL,UFO
- SourceHuman Axl, His Tag (AXL-H5226) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Pro 449 (Accession # AAH32229).Predicted N-terminus: Ala 26Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 46.6 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.

Human Axl, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- Citations
Randomized humanized antibody libraries engineered with sequence and insert diversity
Authors: Goldstein N, et al.
Journal: US20160318995A1 2016
Application: Phage Library Screening
Request for Full-text
- BackgroundAXL Receptor Tyrosine Kinase is also known as Tyrosine-protein kinase receptor UFO, which belongs to the protein kinase superfamily, Tyr protein kinase family and AXL/UFO subfamily. AXL contains two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. AXL is highly expressed in metastatic colon tumors. AXL is activated by GAS6-binding and subsequent autophosphorylation. AXL is involved in signal transduction from the extracellular matrix into the cytoplasm by binding growth factors, and thus implicated in the stimulation of cell proliferation.
- References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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从原理上说,酶标仪是通过对酶标板中液体的吸光值检测,获得一个OD值后进行定性或半定量的分析,达到检测的目的。其优点是仪器简单、价格低廉、方便操作;缺点是灵敏度低、检测范围窄、难以实现自动化等。
化学发光免疫分析仪是化学发光反应(酶促发光或直接发光)产生的光信号通过光电倍增管进行信号转换后等到相应的信号值,用RLU(相对光单位)表示,以达到定量或定性的检测目的。优缺点几乎就是酶标仪的反面。
酶标仪选择重复性好的,线性范围宽的,故障率底的,售后服务跟的上的厂家的仪器就好了。
3.重装系统也可以;
4.如果是Android系统的平板电脑忘记锁屏密码解决方法:可以重新刷包或者wipe data双清数据(注意备份平板电脑数据)。
想请教一下各位大神,为什么我做的Caco-2细胞的药物干预,用酶标仪检测CCK8,5/6孔总是异常,按理说,药物干预后,数据应该是递增或递减的,然而234孔递减,5/6孔却上升了,7、8孔又是递减。请问有人有遇到过这种情况吗??在此先谢谢各位亲了~
少数项目,二者可共同检测。
其次,价格不同,酶标仪相对便宜。
石英的96孔板,每个孔的体积为200ul,空白直接200ulDEPC水,实验组196ulPEPC水+4ulRNA
如果换了滤光片了,测试步骤与MTT测试步骤差不多,主要就是设置吸光度,点击四次吸光度,设置为340、280、260、230。
1、进板后确定板类型和板孔
2、设置吸光度,点击四次吸光度,分别设置为340、280、260、230。
3、开始
酶标仪即酶联免疫检测仪是酶联免疫吸附试验的专用仪器又称微孔板检测器。可简单地分为半自动和全自动2大类,但其工作原理基本上都是一致的,其核心都是一个比色计,即用比色法来进行分析。 测定一般要求测试液的最终体积在250μL以下,用一般光电比色计无法完成测试,因此对酶标仪中的光电比色计有特殊要求。
因为之前一直在找这个软件,很久都没找到,终于在丁香上看到有人共享出来了,嘿嘿,,,,,
高兴的安装完了,Eee..........没序列号,没办法,又找了N多天所有能上的网站都上了都没有。
又想到可以**试,又开始学**,嘿嘿,断断续续的学,一个多月过去终于成功了,不容易啊
现在共享给大家。
各位前辈,MTT法加样后第一天阳性组镜下看细胞全死了,样品组和阴性组镜下状态还可以,加DMSO后样品孔和阴性孔颜色粉红,阳性孔红紫到发黑,酶标仪检测,样品组和阴性组大概0.2-0.4,阳性组却有1.2-1.4,请问,这是怎么回事?细胞接板密度为4*104,100μL。向各位求助了,万分感谢!

