
- SynonymNTRK2,TRKB,GP145-TrkB
- SourceHuman TrkB, His Tag (NT2-H5228) is expressed from human 293 cells (HEK293). It contains AA Cys 32 - His 430 (Accession # AAH31835).Predicted N-terminus: Cys 32Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 45.2 kDa. The protein migrates as 60-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.

Human TrkB, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundNeurotrophic tyrosine kinase receptor type 2 (NTRK2) is also known as BDNF/NT-3 growth factors receptor, Tropomyosin-related kinase B (TRKB) and TrkB tyrosine kinase, which belongs to the protein kinase superfamily or Tyr protein kinase family. Insulin receptor subfamily. NTRK2 / TrkB contains two Ig-like C2-type (immunoglobulin-like) domains, two LRR (leucine-rich) repeats, one LRRCT domain, one LRRNT domain, one protein kinase domain. NTRK2 / Trk-B is expressed in the central and peripheral nervous system. The catalytic activity of NTRK2 is “ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate”. NTRK2 / TrkB involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity.
- References
- (1)Allen S.J., et al., 1994, Neuroscience 60:825-834.
- (2)Stoilov P., et al., 2002, Biochem. Biophys. Res. Commun. 290:1054-1065.
- (3)Meakin S.O., et al., 1999, J. Biol. Chem. 274:9861-9870.
- (4)Yeo G.S., et al., 2004, Nat. Neurosci. 7:1187-1189.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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酶标仪按照功能的不同划分,可以分为(1)光吸收酶标仪(可见酶标仪,紫外/可见酶标仪)(2)荧光酶标仪(3)化学发光酶标仪。
分光光度计按照波长及应用领域的不同可以分为:(1)可见光分光光度计(2)紫外分光光度计(3)红外分光光度计(4)荧光分光光度计(5)原子吸收分光光度计。
分光光度计和酶标板存在一些不同之处,具体差别体现在以下三个方面:
(1)盛装待测溶液的容器:
分光光度计用的是比色皿,酶标仪使用的是塑料微孔板(酶标板)。比色皿只能起到盛装溶液的作用,每个比色皿一次只能盛装一种溶液。酶标板常用透明的聚乙烯材料制成,对抗原抗体有较强的吸附作用,因此用它作为固相载体,酶标板通常为48孔或96孔,每个微孔可以盛装不同的溶液。
(2)光路的方向:
分光光度计是水平光路,而酶标仪则是垂直光路。由于酶标板盛样本的塑料微孔板是多排多孔的,光线只能垂直穿过,因此酶标仪的光束都是垂直通过待测溶液和微孔板的,光束既可是从上到下,也可以是从下到上穿过比色液。垂直光的特点是标本吸光度受液体浓缩或稀释的影响小,不足之处是受被测样本液面是否水平、酶标板透光性、孔底是否平整等的影响较大。
(3)光路的长度:
由于光密度(OD值)与吸光系数, 待测组分的浓度以及光路长度成正比关系。
分光光度计采用的比色杯的宽度通常是1cm,所以光路长度固定为1cm。因此不同仪器,不同批次测量的数据具有同样的可比性。
而酶标仪采用的是垂直光路, 所以光路的长度应该是液体液面的高度。所以测得的值受到样品的体积的影响。
请问BAC蛋白浓度测定波长是562,我们这边酶标仪的滤光片波长最接近的是595,这样也可以测吗
各位前辈,MTT法加样后第一天阳性组镜下看细胞全死了,样品组和阴性组镜下状态还可以,加DMSO后样品孔和阴性孔颜色粉红,阳性孔红紫到发黑,酶标仪检测,样品组和阴性组大概0.2-0.4,阳性组却有1.2-1.4,请问,这是怎么回事?细胞接板密度为4*104,100μL。向各位求助了,万分感谢!

