PP2A2, Protein Phosphatase 2A2
Source: Bovine kidney
Purity: > 90% by SDS-PAGE, subunit apparent Mr’s ~ 36- and 65- kDa
Supplied: In 50 μl 50 mM Tris-HCl pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA and 50% glycerol.
Activity: ~ 2000 units/mg with myelin basic protein (MBP) as substrate. One unit is the amount of PP2A2 that releases 1 nmol of inorganic phosphate from 32P-labeled MBP per min. Maintain preparations in aliquots at -70° C. Avoid repeated thawing.
Synonyms: Protein Phosphatase 2A2; PP2A2
Background: Protein phosphatase 2A (PP2A) is a divalent cation-independent multifunctional protein serine threonine phosphatase. PP2A regulates numerous cellular processes including the cell cycle, growth and differentiation. Physiological targets of PP2A include cell surface receptors and ion channels, and protein kinases involved in mitogenic signaling and the cell cycle. PP2A also acts on numerous transcription factors as well as key regulatory enzymes in metabolism. PP2A is a growth and tumor suppressor. It is inhibited potently by tumor promoters such as okadaic acid. It is also inhibited potently by two cancer-associated cellular proteins, I1PP2A and I2PP2A/SET. In addition, the SV40 small t antigen replaces the B subunit of PP2A during viral transformation. This subverts the physiological function of a subset of PP2A complexes in a substrate selective manner.
Figure: Purified preparation of bovine kidney PP2A2 was subjected to SDS-PAGE. The gel was stained with Coomassie Blue. The 65-kda A (top) and 36-kDa C (bottom) subunits are seen.
Protein Phosphatase 2A2 (PP2A2) is a dimeric form of PP2A. It contains A (65 kDa) and C (36 kDa) subunits but lacks the B subunit present in PP2A1. GloboZymes purified PP2A2 preparations are used to study enzyme kinetics and regulation. They are used to dephosphorylate target substrates and to evaluate the effects of test substances on the activity of the phosphatase. The preparations can also be used for determining the role of the missing B subunit of the enzyme.
References: Biochem J 287: 1019; Proc Natl Acad Sci USA 90: 2500
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
我需要做pcr，做之前用酶标仪测定同一管dna浓度时，数字相差很大，有100度的也有五六十的，这是正常现象么，因为要做正交实验，需要准确的浓度，这种状况，我应该怎么确定浓度呢？求助求助！！
2、检测模式：荧光强度检测(FI), 荧光偏振检测(FP), 时间分辨荧光检测(TRF), TR-FRET, 高性能发光检测, 紫外/可见吸收光, AlphaScreen / AlphaLISA。
3、兼容 Take3™ 微量检测板 可进行体积为2 μL 的微量样品的检测
4、四光栅系统及带宽可调: Synergy H4 的光路整合了两个双光栅单色器. 这种四光栅设计. 可变的带宽设计极大的增加了检测的灵活性。
5、深度阻挡滤光片和二向色镜: Synergy H4的滤光片/二向色镜组合为，荧光强度、时间分辨荧光、荧光偏振及AlphaScreen / AlphaLISA 均具有最好的检测性能.向左转|向右转
想问下各位，南京建成购买的测组织MPO的试剂盒能否用酶标仪测啊？说明书上只写了用比色皿测，但由于比色皿操作比较慢，就在想能不能加在96孔板里用酶标仪测。有经验的小伙伴麻烦分享下经验。如果可以用酶标仪测，那么操作步骤还是按那个比色皿法的说明书来吗，步骤有没有哪里不同的？？感谢各位！
因为之前一直在找这个软件，很久都没找到，终于在丁香上看到有人共享出来了，嘿嘿,,,,,
高兴的安装完了，Eee..........没序列号，没办法，又找了N多天所有能上的网站都上了都没有。
又想到可以**试,又开始学**，嘿嘿，断断续续的学，一个多月过去终于成功了，不容易啊
现在共享给大家。
我们的酶标仪是bio-tekSynergyHT的型号，在测96孔板细胞荧光的时候选择bottom，区域扫描5*5，但是数值出来以后每个孔只读了13个值而不是25个值，why？还有，激发光的光束是圆形的还是正方形的？区域扫描为什么不是整个孔的扫描，而只是中间一个很小的区域扫描？选择bottom和top模式的时候有什么区别？是激发光源一个从底部激发一个从顶部激发的吗？
求大神赐教！
请问BAC蛋白浓度测定波长是562，我们这边酶标仪的滤光片波长最接近的是595，这样也可以测吗
