Recombinant Human His6-MBP-HOIP/RNF31 Catalytic Domain, CF Summary
Recombinant Human HOIP/RNF31 is a RING domain Ubiquitin ligase (E3) and component of the LUBAC complex that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human HOIP/RNF31 concentration of 0.1-0.5 μM.
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E3-240
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 10% Glycerol, 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: HOIP/RNF31
HOIP (HOIL-1 Interacting Protein, also known as RNF31) is a RING domain ubiquitin E3 ligase and component of the LUBAC complex. LUBAC, a large (approximately 600 kDa) heteromultimeric assembly consisting of at least HOIP, HOIL-1, and Sharpin, conjugates linear polyubiquitin chains to various substrates and plays a key role in NF-kappa B activation and signaling events mediated through TNF alphaand IFN. Both the catalytic activity and specificity for linear ubiquitin chain formation of LUBAC are contained within the C-terminal RBR (RING-IBR-RING) and LDD (linear ubiquitin chain determining domain) domains of HOIP. HOIL-1 and Sharpin are essential for full-length HOIP activity, but not for HOIP truncation mutants consisting of the RBR and LDD domains. This protein contains N-terminal 6-His and MBP tags, and is useful for generating unanchored linear polyubiquitin chains without the requirement for HOIL-1 or Sharpin. HOIP catalytic domain contains aa’s 699-1072.
- Belgnaoui S.M., et al. (2012) Cell Host Microbe 12: 211-222
- Smit J.J, et al. (2012) EMBO J. 31: 3833-3844
- Stieglitz B., et al. (2012) EMBO Rep. 13: 840-846
- Wauer T. and Komander D. (2013) EMBO J. doi:10.1038/emboj.2013.125
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酶标仪选择重复性好的，线性范围宽的，故障率底的，售后服务跟的上的厂家的仪器就好了。
想请教一下各位大神，为什么我做的Caco-2细胞的药物干预，用酶标仪检测CCK8,5/6孔总是异常，按理说，药物干预后，数据应该是递增或递减的，然而234孔递减，5/6孔却上升了，7、8孔又是递减。请问有人有遇到过这种情况吗？？在此先谢谢各位亲了~
CSY-E96D动物疾病快速诊断仪采用固相酶联免疫吸附ELISA的原理,即酶联免疫法；可定量快速畜牧类疾病诊断如禽流感、猪瘟、猪蓝耳、伪狂犬等疾病，广泛应用于养殖场、屠宰场、肉产品深加工企业、检验检疫单位使用。
各位前辈，MTT法加样后第一天阳性组镜下看细胞全死了，样品组和阴性组镜下状态还可以，加DMSO后样品孔和阴性孔颜色粉红，阳性孔红紫到发黑，酶标仪检测，样品组和阴性组大概0.2-0.4，阳性组却有1.2-1.4，请问，这是怎么回事？细胞接板密度为4*104,100μL。向各位求助了，万分感谢！
测器和微处理器控制系统等组成.
光源灯发出的光线经过滤光片或单色器后,成为一束单色光.该单色光束经过酶标板中的待测标本,被标本吸收掉一部分后,到达光电检测器.光电检测器将投照到
上面的光信号的强弱转变成电信号的大小.此电信号经前置放大、对数放大、模数转换等处理后,送人微处理器进行数据处理和计算,最后通过显示器和打印机输出
测试结果.
请问BAC蛋白浓度测定波长是562，我们这边酶标仪的滤光片波长最接近的是595，这样也可以测吗
酶标仪有单波长和双波长检测功能有时使用者不知在什么情况下使用单或双波长检测。所谓的“单波长”就是使用一种对显色具最大吸收的波长即450 nm或492 nm进行比色测定；而“双波长”则除了用对显色具最大吸收的波长即450 nm或492 nm进行比色测定外，同时用对特异显色不敏感的波长如630 nm进行测定，酶标仪最后打印出来的吸光度则为二者之差。630 nm波长下得到的吸光度是非特异的，来自于板子上诸如指纹、灰尘、脏物等所致的吸收。因此，在ELISA比色测定中，最好使用双波长，且不必设空白孔。
