Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Plasmid | 100841 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | Add to Cart | |
| AAV1 | 100841-AAV1 | Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information | Add to Cart | |||
| AAV5 | 100841-AAV5 | Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information | Add to Cart | |||
| AAV9 | 100841-AAV9 | Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information | Add to Cart | |||
This material is available to academics and nonprofits only.
Backbone
- Vector backbonepAAV(Search Vector Database)
- Vector typeMammalian Expression, AAV
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)NEB Stable
- Copy numberUnknown
Gene/Insert
- Gene/Insert nameGCaMP6m
- Alt nameGCaMP3 variant 632
- SpeciesSynthetic
- Insert Size (bp)1353
- MutationGCaMP3-T302L R303P M378G K379S D380Y T381R S383T R392G
- PromoterSynapsin
Cloning Information
- Cloning methodUnknown
- 5′ sequencing primerunknown (Common Sequencing Primers)
Resource Information
- Supplemental Documents
- pAAV.Syn.GCaMP6m.WPRE.SV40 (p2823)_Q.gb
- Terms and Licenses
- UBMTA
- Industry Terms
- Not Available to Industry
- Articles Citing this Plasmid
- 2 References
Depositor Comments
This plasmid was previously available as pAAV.Syn.GCaMP6m.WPRE.SV40 (p2823) from the Penn Vector Core. This plasmid was created as part of the GENIE project at Janelia Research Campus.
Information for AAV1 (Catalog # 100841-AAV1)(Back to top)
Purpose
Ready-to-use AAV1 particles produced from pAAV.Syn.GCaMP6m.WPRE.SV40 (#100841). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6m.WPRE.SV40 plasmid DNA.
Syn-driven GCaMP6m calcium sensor. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 1×10¹³ vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV1
- PurificationIodixanol gradient ultracentrifugation
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Information for AAV5 (Catalog # 100841-AAV5)(Back to top)
Purpose
Ready-to-use AAV5 particles produced from pAAV.Syn.GCaMP6m.WPRE.SV40 (#100841). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6m.WPRE.SV40 plasmid DNA.
Syn-driven GCaMP6m calcium sensor. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 7×10¹² vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV5
- PurificationIodixanol gradient ultracentrifugation
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Information for AAV9 (Catalog # 100841-AAV9)(Back to top)
Purpose
Ready-to-use AAV9 particles produced from pAAV.Syn.GCaMP6m.WPRE.SV40 (#100841). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6m.WPRE.SV40 plasmid DNA.
Syn-driven GCaMP6m calcium sensor. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 1×10¹³ vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV9 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV9
- PurificationIodixanol gradient ultracentrifugation
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
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1、光源不同:可见分光光度计的光源一般只用钨灯,而紫外可见分光光度计是用钨灯 氘灯两个光源,同时还多了这两个光源灯的切换部件.这是因为钨灯的光谱范围主要在可见到近红外这段,氘灯主要在紫外端.也正是因为光源的不一样,紫外可见分光光度计也多了一个专门提供氘灯工作的氘灯电源了.
2、光学器件的不同:由于玻璃能吸收紫外波,而对可见到近红外端有比较好的透过性,所以可见分光光度计的一些光学部件可以使用玻璃,而紫外可见分光光度计就不能使用玻璃部件,一般使用石英光学部件.同时由于这个原因,在比色皿的选择上也就有不同了,可见分光光度计可以使用玻璃制的比色皿,而紫外可见分光光度计一般使用石英制的比色皿了.
3、接收器的不同:由于紫外可见分光光度计多了紫外波,所以在接收器的选择上也就不一样了.多了对紫外波的灵敏响应功能,这类接收器的价格就比可见分光光度计的接收器贵了很多了.
①辐射源。必须具有稳定的、有足够输出功率的、能提供仪器使用波段的连续光谱,如钨灯、卤钨灯(波长范围350~2500纳米),氘灯或氢灯(180~460纳米),或可调谐染料激光光源等。
②单色器 。它由入射、出射狭缝、透镜系统和色散元件(棱镜或光栅)组成,是用以产生高纯度单色光束的装置,其功能包括将光源产生的复合光分解为单色光和分出所需的单色光束。
③试样容器,又称吸收池。供盛放试液进行吸光度测量之用,分为石英池和玻璃池两种,前者适用于紫外到可见区,后者只适用于可见区。容器的光程一般为0.5~10厘米。
④检测器,又称光电转换器。常用的有光电管或光电倍增管,后者较前者更灵敏,特别适用于检测较弱的辐射。近年来还使用光导摄像管或光电二极管矩阵作检测器,具有快速扫描的特点。
⑤显示装置。这部分装置发展较快。较高级的光度计,常备有微处理机、荧光屏显示和记录仪等,可将图谱、数据和操作条件都显示出来。
可见分光光度计(又名可见光度计、分光光度计)是可见光分光光度法是上海美析仪器采用最新的单片机技术,开发出能够进行定量测量(标准曲线测量,可对物质进行浓度直读);OD值直接测量(吸光度、透过率和能量等直读);动力学测试(测出物质浓度随时间变化OD值的变化);光谱扫描(可以对某一种物质进行全波段扫描,分析物质的特征波长,判断实验过程的误差);多波长测试(可以对物质同时进行多个波长的测试,分析物质的相关特性);还有可以进行DNA/蛋白质测试、总磷总氮测试、重金属测试、农药残留测试、食品安全检测、热力发电金属离子测试等等,广泛应用于食品、药品、电力、生物研究、教学科研、化学化工、质量监督、水质环保和商检等各大领域。V-1500PC型可见分光光度计,波长范围:320nm-1100nm,能满足不同物质的测试。
3.1透过率、吸收度测量
a.暗电流调零:开启电源后,若试样室盖未打开,将显示提示符P.1(请做第一步骤操作),打开试样室盖直到接触检测开关,提示符P.1消失,显示暗电流值。如果它的绝对值小于5.0,则两秒内自动归零,否则可调节面板上之0%T旋钮。
b.调整参比讯号(100T/OA):微机所显示的提示符为P.2(请做第二步操作)。将T—A—C旋钮开关置T/A,参比溶液放进1号试样槽并移入光路,关闭试样室盖,显示参比讯号强度,调整狭缝和光楔(试样槽拉手左面之100%T旋钮),使显示数在90.0—119.9%T或相应A值范围以内,2秒以内将自动调整为100%T/OA。
c.比色皿误差校正:为校正比色皿的系统误差,要求在同一次测量过程中,比色皿与槽位编号一一照应。将使用的比色皿盛参比溶液放进试样槽,移入光路,所显示的T(或A值)就反映了该与1号槽中比色皿之间的配对误差。按一下ADJ(Adjust)校正键,则误差数据存入微机并自动校正为100%T/OA。这项操作在每次开机后只需作一次,若所用的比色皿配对性很好,可免去这一步骤。开机时,校正系数初始值为1,即相当于不校正。
d.用同比色皿盛被测溶液放进同一试样槽,则显示校正后的透过率或吸光率。
3.2浓度测量
a.标准对照法
b.按3.1节(1)—(3)操作。
c.将标准浓度的样品放如光路。
d.旋钮开关至C,分别按三个浓度置数键,使显示数与已知浓度值一致,浓度值仅取其三为有效数字,小数点位置自定义。
e.将被测式样移入光路,则显示样品的浓度(比色皿误差已校正)。
3.2标准曲线法(标准曲线为直线)
a.按3.1节(1)—(3)操作。
b.旋钮至A, 调整光楔使A值变化至标准曲线高端某一点的坐标A0。
c.旋钮至C,按浓度置数键显示数与对应于A0的浓度值C0一致。
d.重新调整对比讯号后按(1)之(4)操作。
3.3测试完毕,依次关闭主机电源开关,电气箱开关,拔下电源插头,盖上护罩。
3.4认真填写使用操作记录。
紫外分光光度计测出来吸光度需要除以待测液的体积吗
分光光度计已经成为现代分子生物实验室常规仪器。常用于核酸,蛋白定量以及细菌生长浓度的定量。
仪器主要由光源、单色器、样品室、检测器、信号处理器和显示与存储系统组成。
今天,导师问到我一个问题,
HPLC检测器与UV分光光度计的主要区别。
我说HPLC检测器的分光部件在样品流动池的后面,UV分光光度计的分光部件在样品的前面。
导师问我,那么分光部件的前置和后置各有什么优点和缺点呢?
我百思不得其解。
特地前来请教
1,被测液浓度过高造成透过样品的光信号检测不到,这种情况下可以通过稀释样品来完成;
2,测定时,样品室内的样品架在移动时没移动到位造成挡光;
3,仪器内部的光源不亮或光源没有进入单色器中。可以对照以上情况检查分析!
长(频率、或波数),分别测定后,手工绘制光谱图.显然这种仪器对于需要经常根据光谱图定性分析的用户而言是很不方便的,但价格相对于光谱仪要便宜得多,
对只需定量分析的用户就够用了.
1、光谱仪,以光电倍增管等光探测器测量谱线不同波长位置强度的装置。其构造由一个入射狭缝,一个色散系统,一个成像系统和一个或多个出射狭缝组成。以色散元件将辐射源的电磁辐射分离出所需要的波长或波长区域,并在选定的波长上(或扫描某一波段)进行强度测定。分为单色仪和多色仪两种。
2、分光光度计是将成分复杂的光,分解为光谱线的科学仪器。测量范围一般包括波长范围为380~780
nm的可见光区和波长范围为200~380
nm的紫外光区。不同的光源都有其特有的发射光谱,因此可采用不同的发光体作为仪器的光源。钨灯的发射光谱:钨灯光源所发出的380~780nm波长的光
谱光通过三棱镜折射后,可得到由红、橙、黄、绿、蓝、靛、紫组成的连续色谱;该色谱可作为可见光分光光度计的光源。

