Overview
The E-Z 96® Total RNA Kit is designed for isolation of total cellular RNA from up to 5 x 105 cultured cells or soft tissues. This kit can process single or multiple samples in less than 60 minutes. Utilizing HiBind® silica plate technology, the need for phenol/chloroform extractions, CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl are eliminated. Samples are lysed in a denaturing lysis buffer which inactivates RNases. Binding conditions are adjusted and the lysate is transferred to a 96-well HiBind® RNA Plate where the RNA is purified via three wash steps. High-quality RNA is eluted in RNase-free water. RNA purified using the E-Z 96® Total RNA method is ready for applications such as RT-PCR, qPCR, differential display, microarrays, and other downstream applications.
Specifications
For Research Use Only. Not for use in diagnostic procedures.
| Features | Specifications |
|---|---|
| Downstream Application | PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification |
| Elution Volume | 50-75 μL |
| Starting Material | Culture cells |
| Starting Amount | up to 5x105 cells |
| Processing Mode | Manual, centrifugation/vacuum |
| Throughput | 96 samples per run |
| RNA Binding technology | Silica membrane 96-well |
Protocol and Resources
Product Documentation & Literature
PROTOCOL
R1034 E-Z 96® Total RNA Kit
SDS
R1034 SDS
SALES SHEET
Product Data
Total purified RNA using the E-Z 96® Total RNA Kit
Figure 1. Purified total RNA from 10 mg chicken liver was isolated with the E-Z 96® Total RNA Kit. Total RNA (500 ng/lane) was analyzed on a 1% agarose gel to demonstrate yield and quality of the RNA.
