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Mesenchymal Stem Cells (MSC), also termed Mesenchymal Stromal Cells, are multipotent cells that can differentiate into a variety of cell types and have the capacity for self renewal. MSC have been shown to differentiate in vitro or in vivo into adipocytes, chondrocytes, osteoblasts, myocytes, neurons, hepatocytes, and pancreatic islet cells. Optimized PromoCell media are available to support both the growth of MSC and their differentiation into several different lineages. Recent experiments suggest that differentiation capabilities into diverse cell types vary between MSC of different origin.
PromoCell hMSC are harvested from normal human adipose tissue, bone marrow, and umbilical cord matrix (Wharton’s jelly) of individual donors.
The cells are tested for their ability to differentiate in vitro into adipocytes, chondrocytes, and osteoblasts. Our hMSC show a verified marker expression profile that complies with ISCT* recommendations, providing well characterized cells.
*ISCT (International Society for Cellular Therapy) Cytotherapy (2006) Vol. 8, No. 4, 315-317
NEW: Our hMSC are now also available from HLA-typed donors.
Available formats:
- Cryopreserved: Cryogenic vial containing 500.000 viable cells.
- Proliferating: >500.000 viable cells shipped in growth medium (T75 flask).
- Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
| Recommended plating density | 4,000 cells per cm2 |
|---|---|
| Tested Markers | Differentiation capacity to adipogenic, osteogenic and chondrognic cells tested, CD105 positive, CD73 positive, CD90 positive, CD45 negative, CD34 negative, CD14 negative, CD19 negative, HLA-DR negative |














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帮帮忙,谢谢
现在对美国perkinelmer公司和德国berthold公司的产品有兴趣,
perkinelmer公司的样品形式可选用6,12,24.........384空板进样同时检测,比较方便。检测灵敏度为3attomoleATP;
berthold公司的样品形式只有试管单管进样,比较麻烦;但是检测灵敏度为1attomoleATP,灵敏度更高。
请教前辈我该如何选择?请问一般荧光素酶报告基因发光值一般是什么数量级?
MPI是一种基于消息传递的并行编程技术,在不同节点计算机之间并行多进程执行程序(这种情况下,不同享内存),只是通过消息传递来进行通信,从而适用于分布式体系
是将具有高灵敏度的化学发光测定技术与高特异性的免疫反应相结合,用于各种抗原、半抗原、抗体、激素、酶、脂肪酸、维生素和药物等的检测分析技术。是继放免分析、酶免分析、荧光免疫分析和时间分辨荧光免疫分析之后发展起来的一项最新免疫测定技术。

