- SynonymIgG1
- SourceHuman IgG Fc, Tag Free (FCC-H5214) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 330 (Accession # P01857-1).Predicted N-terminus: Glu 99Request for sequence
- Molecular Characterization
This protein carries no "tag".
The protein has a calculated MW of 26.1 kDa. The protein migrates as 32-34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehaloseis added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human IgG Fc, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human CD64, His Tag (Cat. No. FCA-H52H2) at 10μg/mL (100 μL/well) can bind Human IgG Fc, Tag Free (Cat. No. FCC-H5214) with a linear range of 7-41 ng/mL (QC tested).
Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) (Cat. No. FCN-H52W7) captured on CM5 Chip via anti-His antibody can bind Human IgG1 Fc, Tag Free (Cat. No. FCC-H5214) with an affinity constant of 0.957 μM as determined in SPR assay (Biacore 8K) (Routinely tested).
- Citations
Site-Specific Crosslinking of Antibodies
Authors: B Agnew, et al
Journal: US20190177438A1 43629
Application: Fluorescent labeled
Request for Full-text
Compositions and methods for inhibiting WNT signaling
Authors: M Dong, et al
Journal: 20190031734A1 43496
Application: BLI
Request for Full-text
Impact of payload hydrophobicity on stability of antibody-drug-conjugates
Authors: Buecheler JW, et al.
Journal: Mol Pharm 2018
Application: BLI
Request for Full-text
Single chain Fc-dimer-human growth hormone fusion protein for improved drug delivery
Authors: Li Zhou, et al.
Journal: Biomaterials 2016
Application: As a control in Cell-based assay
Request for Full-text
Frizzled proteins are colonic epithelial receptors for C. difficile toxin B
Authors: Tao L, et al.
Journal: Nature 2016
Application: As a control in BLI assay
Request for Full-text
Multifunctional RNA nanoparticles
Authors: Kirill A. Afonin, et al.
Journal: Nano Lett 2014
Application: As a control in Cell-based assay
Request for Full-text
- BackgroundCrystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.
- References
- (1)Anthony RM, et al., 2010, J Clin Immunol, 30 Suppl 1:S9-14.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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凝胶成像仪genegeniusbioimage（syngene公司）系统，我实验室是2006年直接进口了这台仪器，时间太久远，连工程师的号码都是空号了，用过这台仪器的师兄师姐也都毕业不知道了找谁了，请问哪位实验室有这个公司产品的，找一下武汉这边的负责人，我们想联系到再找工程师来维修一下，谢谢！
2．打开电脑，打开并进入成像软件。
ECL拍摄
将拍摄模式切换为“ECL模式”，将滤光轮转到ECL位。选择合适拍摄分辨率（有像素合并功能的机器都有这个功能），点击“启动”。将样品放置在样品台正中间，调整镜头的焦距使样品占据窗口约80%左右，,然后点击“自动曝光”，勾掉“负片”并调整聚焦使预览窗口中的样品图像清晰.（光圈越大，自动曝光所需时间越短。）并先用单帧拍摄，拍摄一张Mark照片。关闭反射白光后给放置在化学发光成像板上的硝酸纤维素膜均匀加上发光液。将拍摄方式设置为“规则积分”，勾上“负片”，并设置的时间和张数，点击“拍摄”按钮即可。
普通凝胶拍摄
1．将拍摄模式切换为“普通模式”，将滤光轮转到UV位（无绿光镜轮的无需调整）。
2．选择合适拍摄分辨率（机器有像素合并功能），点击“启动”。
3. DNA胶拍摄：将DNA胶放置在紫外台正中间，调整焦距使样品占据窗口约80%左右，,然后点击“自动曝光”，并调整聚焦使预览窗口中的样品图像清晰.然后关闭反射白光，开启透射紫外，并微调，确保在紫外下处于清晰状态。
蛋白质胶拍摄：将蛋白质胶放在拆叠白光板的中间，关闭反射白光，开启透射白光，然后点击“自动曝光”，并调整聚焦使预览窗口中的样品图像清晰.。
4.在软件界面点击“拍摄”按钮即可。向左转|向右转
