
Recombinant Human HR23A Tandem UBA (TUBE1) Agarose, CF Summary
Human HR23A/Rad23A has two Ubiquitin-associated (UBA) motifs that can each bind Ubiquitin via a hydrophobic surface formed by residues located within thealpha 1 andalpha 3 helices of each UBA domain (1,2). Tandem-repeated Ubiquitin binding entities (TUBEs), which consist of multiple tandem Ubiquitin-binding UBA motifs, have been developed for the isolation and identification of ubiquitinated proteins (3). TUBEs show increased affinity for poly-Ubiquitin moieties compared to single UBA motifs (4). Additionally, TUBEs protect polyubiquitinated proteins from deubiquitylating enzymes, allowing for the detection of polyubiquitinated proteins at relatively low levels of abundance (5).
This affinity resin can be used for the enrichment, isolation and identification of K63-linked (preferentially) or K48-linked poly-Ub chains or ubiquitinated substrates that contain these linkages.
Specifications
- 12 months from date of receipt, 2 to 8 °C as supplied.
- 3 months, 2 to 8 °C under sterile conditions after opening.
Product Datasheets
Background: HR23A/Rad23A TUBE1
- Chen, L. et al. (2001) EMBO Rep. 2:933.
- Wang, Q. et al. (2003) Biochemistry 42:13529.
- Hjerpe, R. et al. (2009) EMBO Rep. 10:1250.
- Hurley, J.H. et al. (2006) Biochem. J. 399:361.
- Lopitz-Otsoa, F. et al. (2010) Biochem. Soc. Trans. 38:40.
Reagents Provided
FAQs
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浓度的测定,需测定在230、260和280nm处的消光值,经验数据表明,纯净的核酸溶液A260/A230的消光值比大于或等于2.0;A260/A280大于或等于1.80。A260/A280值过小,说明蛋白未脱净;A260/A230过小,说明有杂质(一般为多酚类或色素)。
这样的提问没有意义
建议自己下去查查资料
1.法国Vilber公司,Chemismart3000或2000,
2.美国alpha公司,FluorChemFC2
3.富士的LAS4000
因为没有具体使用过,所以对这些仪器不是很了解,仅从一些数据上看,又不明白具体的实际意义,有哪位可以帮忙推荐一下么??
最好能仔细说说仪器的优缺点.我们需要仪器可以测量DNA电泳(sybr-green)结果和WB结果.
目前只要是在前两个仪器中选择.
谢谢!!!

