96-well Cell Adhesion Assay
(Laminin Coated, Colorimetric Assay)
Catalog Number: ECM-1015, 96 assays
Storage: Upon arrival store the components at 4C.
Introduction: Due to its diverse nature and composition, the ECM can serve many functions, such as providing support and anchorage for cells, segregating tissues from one another, and regulating intercellular communication. The ECM regulates a cell"s dynamic behavior. In addition, it sequesters a wide range of cellular growth factors, and acts as a local depot for them. Changes in physiological conditions can trigger protease activities that cause local release of such depots. This allows the rapid and local growth factor-mediated activation of cellular functions, without de novo synthesis.
Formation of the extracellular matrix is essential for processes like growth, wound healing and fibrosis. An understanding of ECM structure and composition also helps in comprehending the complex dynamics of tumor invasion and metastasis in cancer biology as metastasis often involves the destruction of extracellular matrix[3] by enzymes such as serine and Threonine proteases and Matrix metalloproteinase.
BioPioneer Cell Adhesion Microplate Assays are designed for the determination of the relative attachment of adherent cell lines to extracellular matrix proteins such as Human Fibronectin, Human Vitronectin and Human Collagen IV.
Principle of the assay
BioPioneer 96-well Cell Adhesion Assays are designed for the determination of cell attachment to ECM proteins. After incubation followed by a brief wash step, attached cells are quantified with the dye. BSA-coated wells serve as a negative control, and poly-L-lysine-coated wells serve as a positive control for general attachment.
Kit Components
1. Laminin Adhesion Plate (Part No. ECM-10115): One 96-well plate containing 72 Bovine Laminin coated wells , 8 BSA-coated wells and 8 poly-lysine coated wells(see layout below)
2. Cell Stain Solution (Part No. 10001): One Bottle 1.0ml
3. Extraction Solution (Part No. 10002): One Bottle10.0ml
Materials Not Supplied
1. Cell culture medium
2. Serum free medium, such as DMEM containing 0.5% BSA, 2 mM CaCl2 and 2 mM MgCl2
4. 1X PBS
5. 96-well microtiter plate
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美国UVP凝胶成像分析系统
主要特征
◆ 多款CCD可供用户选择搭配，适用于所有成像实验对摄像系统的要求
◆ F 1.2保证大进光量和高成像速度
◆ 自动调焦，软件控制进行焦距、光圈、滤光片和放大缩小等功能调节
◆ 暗箱密闭性非常好，给化学发光成像提供了最佳条件
◆ 凝胶视窗，装有保护玻璃，无需开门便可快速观察样品
◆ USB接口，使数据快捷而简便传输
◆ 当暗箱门打开，安全保险机制自动关闭透射仪，保证无紫外泄露
◆ 样品台可以由软件控制上下调节，寻找最佳拍摄效果，同时可以记录上次拍摄位置
◆ 提供最好的重现性
◆ 根据不同发射波长要求，采取了五位滤片轮设计，由软件控制滤光片的变换
◆ 可选配外接卤素光源，配置不同的激发光滤光片用于不同荧光成像分析
VisionWorksLS图像采集和分析软件
VisionWorksLS是一套成熟的图像采集及分析软件，其中包含多种工具使拍摄化学发光，荧光，或者比色实验 （染色胶、菌落、斑点和膜）的图像更加简单快捷.
功能包括：
◆ 多种图像拍摄方式一键式图像拍摄、连续拍摄、动态模式拍摄
◆ 图像优化，添加注释
◆ 1D分析、密度分析和菌落计数
◆ 多用户权限设置，保证软件数据安全
◆ 用户可自定义模板与参数设定
◆ 生成报告
◆ 符合FDA 21 CFR Part 11 方式表达
◆ 数据报告可直接生成Excel电子表格
分析功能的应用：
◆ 1D 电泳凝胶分析
◆ Dot blot 分析
◆ 活体动物及植物分析
◆ 菌落计算
◆ 分子量定量
◆ GFP 表达分析
◆ 蛋白定量分析
◆ PCR 基因表达
◆ PCR 定量
◆ TLC 分析
◆ Western blot 密度分析
作用：
小动物活体成像
肿瘤研究
癌症研究
心脏病
基因治疗
（1）普通凝胶成像分析系统：可以对蛋白电泳凝胶，DNA凝胶样品进行图象采集并进行定性和定量分析，样品包括：EB、SYBR Green、SYBR Gold、Texas Red、GelStar、Fluoroscecin、 Radiant Red等染色的核酸监测；以及Coomassie Blue、SYPRO Orange、各种染色的蛋白质凝胶如考染等。（或UV,EB和有色及可见样品成像）；（2）化学发光成像分析系统：成像范围涵盖UV,EB,化学发光、紫外-荧光、有色及可见样品成像；（3）多色荧光成像分析系统：成像范围涵盖UV,EB,化学发光、多色荧光荧光、有色及可见样品成像；（4）多功能活体成像分析系统：UV,EB,化学发光、多色荧光荧光、有色及可见样品成像和离体组织和小型动物，及大型型动物。
我知道所以你知道！
如果有，具体在哪个学校/单位/企业？
最近实验需要用到此设备。
如能告诉，不胜感谢！！！谢谢！！！
