Overview
Product name
Anti-mouse IgG for IP (HRP)See all IgG secondary antibodiesHost species
RatTarget species
MouseTested applications
Suitable for:WBmore detailsConjugation
HRP
Properties
Form
LiquidStorage instructions
Shipped at 4°C. Store at +4°C.Storage buffer
pH: 7.20Constituents: 0.16% Sodium phosphate, 0.88% Sodium chloride, 50% Glycerol, 0.1% BSA
Concentration information loading...Clonality
MonoclonalIsotype
IgGGeneral notes
Number of blots: At least 20 blots, based on a 1 µl/ml dilutionand 5 ml diluted antibody per blot. Important protocol notes: 1. Theanti-mouse IgG for IP secondary antibody (HRP) detects mouse IgG antibodies (subtypes: IgG1, IgG2a, IgG2b, IgG3).2. The anti-mouse IgG for IP secondary antibody (HRP) preferentially detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) over the reduced, SDS-denatured forms.3. IP sample should be completely reduced/denatured before loaded onto a western blot.4. Milk should be used as the blocking protein for the immunoblot.Western blot and IP resources: a) Western blot a beginner"s guide b) IP protocol c) IP troubleshooting tips
Research areas
- Immunology
- Immunoglobulins
- Heavy Chain
- IgG
- Secondary antibodies
- anti-Mouse
- IgG
- Enzyme
- HRP
- Isotype/Loading Controls
- Isotype Controls
- Mouse
Associated products
Related Products
- Anti-mouse IgG for IP (Biotin) (ab131367)
- Goat Anti-Mouse IgG H&L (HRP) (ab97023)
- Rabbit Anti-Mouse IgG H&L (HRP) (ab97046)
- Rat monoclonal [JC5-1] Anti-Mouse lambda light chain (HRP) (ab99625)
Applications
Our Abpromise guarantee covers the use ofab131368in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. The optimal dilution will depend on the sensitivity of the HRP substrate. |
Images
- Western blot - Anti-mouse IgG for IP (HRP) (ab131368)
IP sample preparation: Caspase 7 was immunoprecipitated from 0.5 ml of 1x107 Jurkat (Human T cell leukemia cell line from peripheral blood) cells/ml with 5µg mouse anti-human Caspase 7.
WB conditions: Precipitate from 1x106 cells was subjected to electrophoresis, transferred to an PVDF membrane, and immunoblotted with an anti-Caspase 7 antibody.
Detection: Lane 1: Detection with anti-mouse IgG for IP secondary antibody (HRP) (ab131368) Lane 2: Detection with an HRP-conjugated anti-mouse IgG H&L secondary antibodyLane 3: Lane 1 was re-immunoblotted using an HRP-conjugated anti-mouse IgG H&L secondary antibody. The heavy and light-chains can now be seen, confirming that although the immunoprecipitating heavy and light-chains are present, ab131368 detects only native antibody and not denatured heavy and light-chains.
Please note the detection of the heavy and light-chains of the immunoprecipitating antibody in Lane 2 but not in Lane 1.
Protocols
- Protocol Booklet
Click here to view the general protocols
Datasheets and documents
- Datasheet
- SDS
References
ab131368 has been referenced in 82 publications.
- Luo Z et al. Silencing of RBP-JK promotes the differentiation of bone marrow mesenchymal stem cells into vascular endothelial cells. Mol Med Rep 21:69-76 (2020). PubMed: 31746399
- Zhang Z et al. LncRNA DRAIC inhibits proliferation and metastasis of gastric cancer cells through interfering with NFRKB deubiquitination mediated by UCHL5. Cell Mol Biol Lett 25:29 (2020). PubMed: 32351584
- Kouloulia S et al. Raptor-Mediated Proteasomal Degradation of Deamidated 4E-BP2 Regulates Postnatal Neuronal Translation and NF-?B Activity. Cell Rep 29:3620-3635.e7 (2019). PubMed: 31825840
- Anczurowski M et al. Chaperones of the class I peptide-loading complex facilitate the constitutive presentation of endogenous antigens on HLA-DP84GGPM87. J Autoimmun 102:114-125 (2019). PubMed: 31078377
- Wei X et al. miR-21 inhibitor facilitates the anticancer activity of doxorubicin loaded nanometer in melanoma. Oncol Rep 42:414-424 (2019). PubMed: 31115580
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总局关于发布医用磁共振成像系统临床评价等4项医疗器械注册技术审查指导原则的通告（2017年第6号）
2017年01月16日发布http://www.sfda.gov.cn/WS01/CL0087/168596.html
为加强医疗器械产品注册工作的监督和指导，进一步提高注册审查质量，国家食品药品监督管理总局组织制定了《医用磁共振成像系统临床评价技术审查指导原则》《口腔颌面锥形束计算机体层摄影设备注册技术审查指导原则》《体外除颤产品注册技术审查指导原则》《光固化机注册技术审查指导原则》（见附件），现予发布。
特此通告。
附件：1.医用磁共振成像系统临床评价技术审查指导原则
2.口腔颌面锥形束计算机体层摄影设备注册技术审查指导原则
3.体外除颤产品注册技术审查指导原则
4.光固化机注册技术审查指导原则
食品药品监管总局
2017年1月10日
2017年第6号通告附件1.docx
2017年第6号通告附件2.docx
2017年第6号通告附件3.doc
2017年第6号通告附件4.docx
显微镜是OlympusDP71，弄比例尺的时候出来个“衸”，这是什么鬼啊？如何换算成微米？测量的那个选项我选的是10微米，也只有那个选项可以点。然后倍数选择200，然后他就出来这个200衸，选100倍数是500衸，400倍是200也衸，但长度是200的两倍，**各位大神帮帮忙，要怎样才能换成微米？另外1衸是多少微米？
光系统设计难各种光系统都各自特点所像质优化重点全致 没像数或者物理领域种著名课题 希望能帮
实验室新组建，想咨询一下Camag薄层色谱成像系统的价格及一套显微成像的设备清单与价格。显微成像主要用于中药材的显微鉴别
图1 光声成像工程 (a)光声信号激发与探测；(b)光声成像实现过程示意图
光声成像过程可以分为三个部分：信号的产生、信号的接收和信号处理及图像重建（见图1）。由于脉冲激光器具有光声转换效率高的优点，因此通常被作为光声成像研究中产生信号的激励源。脉冲激光器发出的激光束照射在待研究组织样品上，由于组织样品的吸收效应，在样品内部形成了与组织光学参数相关的能量沉积分布。由于激光脉宽很窄（ns）吸收的能量不能在短时间内释放，导致瞬间温度变化，从而通过热弹机制转化为热膨胀。周期性热流使周围的介质热胀冷缩而激发超声波，由于这种超声波信号的特殊产生机理，为了区别于其它的超声信号，通常称为光声信号。利用超声探测器接收光声信号并对采集到的信号进行适当地处理和采用相应的图像重建算法，就能够得到样品内部光能量沉积的分布。当保证入射光的均匀性的前提下，光声重建图像与吸收分布具有一一对应的关系。向左转|向右转
请问成像系统工作站请问那家的产品做得比较好以及专业一些呢？
