25-OH Vitamin D ELISA Assay Kit
25-OH Vitamin D ELISA Developed and Manufactured in Germany by DLD Diagnostika
Size: 1×96 wells
Sensitivity: 1.6 ng/mL
Dynamic Range: 4 – 120 ng/mL
Incubation Time: 2 hours, 45 minutes
Sample Type: Serum, Plasma
Sample Size: 20 µL
Alternative Names: 25-OH, Vitamin D, 25-hydroxy Vitamin D
For Research Use Only
Controls Included
Reference Range
8.2 to 37.4 ng/ml
It is suggested that each laboratory establish its own normal ranges
Calculation
25-OH Vitamin D3 (ng/mL) x 2.5 = 25-OH Vitamin D3 (nmol/L)
Assay Background
The 25-OH Vitamin D ELISA assay kit is designed for the serological determination of the vitamin D concentration in the human organism types of vitamin D that are differentiated are vitamin D2 (ergocalciferol) that is contained in plant food (mushrooms, avocado) and vitamin D3 (cholecalciferol) that is produced from 7-dehydrocholesterol in the skin under ultra-violet irradiation or found in animal food or products (sea fish, egg yolk, butter) [1, 2, 3, 4]. These two forms of vitamin D, which are not yet biologically active, are bound by a protein called VDBP (vitamin D binding protein) in the bloodstream, then metabolised in the liver and converted into 25-OH vitamin D2 (calcidiol) and 25-OH vitamin D3 (calcitriol), respectively, which are circulating forms of the vitamin with little activity [1]. In contrast to other commercially available tests, the Eagle Biosciences 25-OH Vitamin D ELISA assay kit uses a newly designed monoclonal antibody which is specific for both vitamin D2 and vitamin D3 at 100% specificity. This is necessary because sometimes vitamin D2 instead of D3 is used in therapy [5, 6, 7].
The new 25-OH Vitamin D ELISA assay test kit is designed for the determination of 25-OH Vitamin D in human serum or plasma samples. In the first analysis step, the calibrators and patient samples are diluted with biotin-labelled 25-OH vitamin D and added to microplate wells coated with monoclonal anti-25-OH Vitamin D antibodies. During the incubation an unknown amount of 25-OH Vitamin D in the patient sample and a known amount of biotin-labelled 25-OH vitamin D compete for the antibody binding sites in the microplate wells plate. Unbound 25-OH vitamin D is removed by washing. For the detection of bound biotin-labelled 25-OH vitamin D, a second incubation is performed using peroxidase-labelled streptavidin. In a third incubation using the peroxidase substrate tetramethylbenzidine (TMB) the bound peroxidase promotes a color reaction. The color intensity is inversely proportional to the 25-OH vitamin D concentration. D concentration.
Related Products
Mouse Rat 25-OH Vitamin D ELISA Assay
Multispecies 25-OH Vitamin D ELISA Assay Kit
25-OH Vitamin D HPLC Assay Kit
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
加入溶液把搅拌子放在烧杯溶液中，将控温探头固定在橡胶夹头上，加热头插入至溶液中，不能影响到搅拌子，然后先插上仪器接插的电源插头，再接通电源打开电源调速开关，指标灯亮，即开始工作。
调速是由低速逐步调至高速，不允许高速档直接起动，以免搅拌子不同步，引起跳动。加热有开关控制，打开加热源开关，选择所需的温度，绿灯亮表明加热盘工作，红灯亮表明停止加热盘工作，此时进入恒温状态，不工作时应切断电源，为确保安全，使用时请接上地线．仪器应保持清洁干燥，严禁溶液进入机内，以免损坏机件防止剧烈震动．向左转|向右转
如下图示
问题是：有没有驱动和罐体分开的，像实验室用的那种，如下图：
后者使用移动配液罐，方便灭菌。
请问各位大神，做注射液中试时所用到磁力搅拌器需要资质吗？
小弟好苦恼啊，求各位老师指点迷津！
版主shitou0307留言:
鼓励
用一个微型电动机驱动一块磁钢转动，利用磁钢转动所产生的旋转磁场带动搅拌器托盘上玻璃容器内的搅拌转子转动，从而达到搅拌溶液的目的。
(2)磁力搅拌器的结构
磁力搅拌器是由电动机、磁钢、搅拌转子、搅拌器托盘、调运装置等部分组成的。金属托盘用丁放置化学电池，此外没有用于夹持测量电极和参比电极的电极架。托盘下安装一块永久磁钢，连接在电动机的转动轴L，电动机的旋转带动永久磁钢旋转，利用其磁力吸引搅拌转于旋转，从而起到搅拌作用。如果磁力搅拌器具有加热功能，则还具有电阻加热丝和云母绝缘层。搅拌转于也称磁于p通常是用玻璃管或塑料管密封的小铁棒(避免与溶液起反应)，搅拌转子随磁钢转动而转动。面板装配有调速旋钮、控温加热开关、指不灯等，以实现接通电源、控制磁钢转动和加热的目的。
参考资料:Hi.baidu/=。www.jingda17.net。DI
裂解液配方：
尿素7M
硫脲2M
CHAPS4%
二次水至20ml
使用前加DTT至65mM，tris至40mM，PMSF至1mM
上样液配方：
尿素8M
CHAPS2%
1%溴酚蓝0.002%
二次水至10ml
使用前加DTT至18mM，IPGbuffer至0.5%
由于都含有尿素，特别难溶解，好像含有尿素的温度不能超过37度，就在磁力搅拌器上一直搅着，发现过了一两个小时还没溶解，怎么回事么？中间还在34度左右水浴锅里水浴过，还是没溶解，溅在烧杯壁上的液体都已经结晶了。
有人碰到这样的问题么，很着急，急求帮助。
1.裂解液和上样液配方有无问题？
2.尿素怎么老不容，有没有什么方法加速其溶解？
