easYmer HLA-B*46:01 MHC Tetramers Kit
easYmer HLA-B*46:01 MHC Tetramers Developed and Manufactured by immunAware
Materials Included:
The easYmer kit contains a peptide receptive preparation of HLA, a folding buffer, a positive control peptide for HLA-complex folding. The exact identities of these components are given below:
- easYmer: HLA-B*46:01 – peptide receptive, biotinylated in Tris/Maleate pH 7 with 30% Glycerol
- Folding Buffer: Tris/Maleate pH7
- Peptide: SAFGGGNAF, a stable HLA-B*46:01 binder. Positive control for evaluation analysis of peptide-HLA folding.
For Research Use Only
Available Sizes:
- 20 Tests (Sample-size)
- 50 Tests (Standard Size)
- 150 Tests
- 500 Tests
Key Benefits of easYmer® MHC Tetramers
- Ready-to-use
- One step loading
- Completely flexible and customizable*
- Biotinylated
- No special equipment needed
- Long shelf-life
*For Custom Tetramer Production, please Contact Us for more information
Assay Principle
This easYmer HLA-B*46:01 MHC Tetramers protocol is designed to evaluate the efficiency of peptide-HLA-I interaction and complex formation. The assay is based on detecting the ß2-microglobulin (ß2m? light chain subunit of recombinant HLA Class I (HLA-I) comlexes, where the heavy chain has been biotin tagged. These tagged complexes are subsequently captured by streptavidin coated beads, labeled with PE-conjugated anti-human ß2m, and analyzed by flow cytometry. Since peptide-HLA-I complex formation is entirely peptide dependent, bead-associated signals will only be detected if the peptide in question supports the folding of the HLA-I allotype of interest; peptides that efficiently support folding will give strong signals whereas peptides that support folding sub-optimally, or not at all, will give moderate to non-detectable signals.
Products Related to easYmer HLA-B*46:01 MHC Tetramers
easYmer HLA-B*55:01 MHC Tetramers Kit
easYmer HLA-B*57:02 MHC Tetramers Kit
easYmer HLA-B*15:01 MHC Tetramers Kit
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产品名称:磁力搅拌器ST5ST10
产品品牌:韩国FINEPCR
产品类别:磁力搅拌器
产品详细参数
外形小巧,性能卓越
不带加热的多点磁力搅拌器
高品质的无碳刷电机可提供安静而强有力的搅拌
搅拌转速从0—1300rpm可调
不锈钢表台可防止液体喷溅
外观小巧,操作安全简便
性能参数:
型号
ST5
ST10
转速范围
0—1300rpm
搅拌点间距离
90mm
容量
单点搅拌:2L
250ml*5
250ml*10
材质
表台:不锈钢
重量
3Kg
5Kg
尺寸大小(W*D*H)
114*564*63mm
197*564*63mm
功率
15W
30W
电源
110V/220V/230V,50/60Hz
更多详情请登录:http://www.sci-bridge.com/ProductsList.aspx?pid=186
2、可以设置搅拌速度和温度,同时显示实际速度和产品温度。
3、温度控制精度正负1度左右。速度控制精度正负30转。
4、国产、进口都可以。
5、价格:1万以内。
希望大家给提供几个好品牌,谢谢大家!
第一、在第一次使用时,先对照仪器说明书检查仪器所带配件是否齐全,譬如搅拌子、电源线等;
第二、调速时应由低速逐步调至高速,最好不要高速档直接起动,以免搅拌子不同步,引起跳动;
第三、中速运转可延长搅拌器的使用寿命;
第四、仪器应保持清洁干燥,尤其不要使溶液进入机内;
第五、搅拌时如果发现搅拌子跳动或不搅拌,请检查一下烧杯是否平稳,位置是否正;
第六、不搅拌时不能加热,不工作时应切断电源;
第七、使用时最好能够接上地线。
参考资料:Hi.baidu/=。www.jingda17.cn。DI
本人想买一台制备胶体金的加热磁力搅拌器,大家帮忙推荐下什么品牌的较好,谢谢
谢谢!
不知道有没有卖适合我用的搅拌棒,有的话买一个也行!
在园里查找有一个hbchendl战友提供的方法:
用钳子把小铁钉的两头给剪下来,毛滴管切割成适当的长度,短棒可用细的一头,长棒就用粗的部分,将铁钉塞进毛滴管中,再将两头用酒精灯火给烧死,就可以用了。
0.5cm的得用细铁钉,3cm的搅拌棒得用粗一点的铁钉。
对于这个方法,有一个问题,什么是毛滴管,用什么切割?谢谢
小弟好苦恼啊,求各位老师指点迷津!
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