Recombinant Human Penta-Ub/Ub5 WT Chains (K48-linked), CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
UC-216B
| Formulation | 1 mg/ml (23 μM) in sterile, deionized water |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Penta-Ubiquitin
With a predicted molecular weight of 43 kDa, penta-Ubiquitin chains are composed of five Ubiquitin monomers that are covalently linked through isopeptide bonds, which typically form between a lysine residue of one Ubiquitin molecule and the C-terminal glycine residue of another Ubiquitin molecule (1). Each human Ubiquitin monomer is 76 amino acids (aa) in length and shares 96% and 100% aa identity with yeast and mouse Ubiquitin, respectively (2). Seven of the 76 aa in Ubiquitin are lysine residues that can participate in poly-Ubiquitin chain formation. Linkage through each of the lysine residues is thought to serve as a signal that affects protein degradation, signaling, trafficking, and other cellular processes (3-8).
Linkage specific penta-Ub can be used to investigate mechanism of binding and recognition by E1 or E2 enzymes, deubiquitinating enzymes, E3 ligases, the proteasome or other proteins that contain Ubiquitin-associated domains (UBAs) or Ubiquitin-interacting motifs (UIMs). This product is formed with wild-type ubiquitin and linkage-specific enzymes. It has been shown that the rate of Ubiquitin-substrate conjugate degradation is related to poly-Ubiquitin chain length. Tetra-Ubiquitin is the minimal unit required for recognition by the proteasome, and longer chains probably have enhanced binding to proteasomal subunits and may be more resistant to disassembly by a proteasome-associated isopeptidases.
- Scheffner, M. et al. (1995) Nature 373:81.
- Sharp, P.M. & W.-H. Li (1987) Trends Ecol. Evol. 2:328.
- Behrends, C. & J.W. Harper (2011) Nat. Struct. Mol. Biol. 18:520.
- Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
- Henry, A.G. et al. (2012) Dev. Cell 23:519.
- Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
- Wei, W. et al. (2004) Nature 428:194.
- Zhang, J. et al. (2012) J. Biol. Chem. 287:28646.
Citations for Recombinant Human Penta-Ub/Ub5 WT Chains (K48-linked), CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
2Citations: Showing 1 - 2Filter your results:
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- ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome StabilityAuthors: P Haahr, N Borgermann, X Guo, D Typas, D Achuthanku, S Hoffmann, R Shearer, TK Sixma, N MailandMol. Cell, 2018;0(0):.Species: HumanSample Types: Cell LysatesApplications: Bioassay
- Identification Of Small Molecule TRABID Deubiquitinase Inhibitors ByComputation-Based Virtual Screen.Authors: Shi T, Bao J, Wang N, Zheng J, Wu DBMC Chem Biol, 2012;12(1):4.Species: N/ASample Types: Recombinant ProteinApplications: Bioassay
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如下图示
问题是：有没有驱动和罐体分开的，像实验室用的那种，如下图：
后者使用移动配液罐，方便灭菌。
用一个微型电动机驱动一块磁钢转动，利用磁钢转动所产生的旋转磁场带动搅拌器托盘上玻璃容器内的搅拌转子转动，从而达到搅拌溶液的目的。
(2)磁力搅拌器的结构
磁力搅拌器是由电动机、磁钢、搅拌转子、搅拌器托盘、调运装置等部分组成的。金属托盘用丁放置化学电池，此外没有用于夹持测量电极和参比电极的电极架。托盘下安装一块永久磁钢，连接在电动机的转动轴L，电动机的旋转带动永久磁钢旋转，利用其磁力吸引搅拌转于旋转，从而起到搅拌作用。如果磁力搅拌器具有加热功能，则还具有电阻加热丝和云母绝缘层。搅拌转于也称磁于p通常是用玻璃管或塑料管密封的小铁棒(避免与溶液起反应)，搅拌转子随磁钢转动而转动。面板装配有调速旋钮、控温加热开关、指不灯等，以实现接通电源、控制磁钢转动和加热的目的。
参考资料:Hi.baidu/=。www.jingda17.net。DI
本人想买一台制备胶体金的加热磁力搅拌器，大家帮忙推荐下什么品牌的较好，谢谢
2、可以设置搅拌速度和温度，同时显示实际速度和产品温度。
3、温度控制精度正负1度左右。速度控制精度正负30转。
4、国产、进口都可以。
5、价格：1万以内。
希望大家给提供几个好品牌，谢谢大家！
产品名称：磁力搅拌器ST5ST10
产品品牌：韩国FINEPCR
产品类别：磁力搅拌器
产品详细参数
外形小巧，性能卓越
不带加热的多点磁力搅拌器
高品质的无碳刷电机可提供安静而强有力的搅拌
搅拌转速从0—1300rpm可调
不锈钢表台可防止液体喷溅
外观小巧，操作安全简便
性能参数：
型号
ST5
ST10
转速范围
0—1300rpm
搅拌点间距离
90mm
容量
单点搅拌：2L
250ml*5
250ml*10
材质
表台：不锈钢
重量
3Kg
5Kg
尺寸大小（W*D*H）
114*564*63mm
197*564*63mm
功率
15W
30W
电源
110V/220V/230V,50/60Hz
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就是价格高一般都都2000左右，普通厂家国产一般在200左右，具体看参数，磁力搅拌器一以电磁物理特性工作，所以一般个人用推荐就买普通国产就行，对于实验室要求较高的大型实验室，可购买进口类，不过现在很多国产也有很不错的价格一般在进口半价左右。
