Recombinant Human Penta-Ub/Ub5 WT Chains (K48-linked), CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
UC-216B
| Formulation | 1 mg/ml (23 μM) in sterile, deionized water |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Penta-Ubiquitin
With a predicted molecular weight of 43 kDa, penta-Ubiquitin chains are composed of five Ubiquitin monomers that are covalently linked through isopeptide bonds, which typically form between a lysine residue of one Ubiquitin molecule and the C-terminal glycine residue of another Ubiquitin molecule (1). Each human Ubiquitin monomer is 76 amino acids (aa) in length and shares 96% and 100% aa identity with yeast and mouse Ubiquitin, respectively (2). Seven of the 76 aa in Ubiquitin are lysine residues that can participate in poly-Ubiquitin chain formation. Linkage through each of the lysine residues is thought to serve as a signal that affects protein degradation, signaling, trafficking, and other cellular processes (3-8).
Linkage specific penta-Ub can be used to investigate mechanism of binding and recognition by E1 or E2 enzymes, deubiquitinating enzymes, E3 ligases, the proteasome or other proteins that contain Ubiquitin-associated domains (UBAs) or Ubiquitin-interacting motifs (UIMs). This product is formed with wild-type ubiquitin and linkage-specific enzymes. It has been shown that the rate of Ubiquitin-substrate conjugate degradation is related to poly-Ubiquitin chain length. Tetra-Ubiquitin is the minimal unit required for recognition by the proteasome, and longer chains probably have enhanced binding to proteasomal subunits and may be more resistant to disassembly by a proteasome-associated isopeptidases.
- Scheffner, M. et al. (1995) Nature 373:81.
- Sharp, P.M. & W.-H. Li (1987) Trends Ecol. Evol. 2:328.
- Behrends, C. & J.W. Harper (2011) Nat. Struct. Mol. Biol. 18:520.
- Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
- Henry, A.G. et al. (2012) Dev. Cell 23:519.
- Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
- Wei, W. et al. (2004) Nature 428:194.
- Zhang, J. et al. (2012) J. Biol. Chem. 287:28646.
Citations for Recombinant Human Penta-Ub/Ub5 WT Chains (K48-linked), CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
2Citations: Showing 1 - 2Filter your results:
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- ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome StabilityAuthors: P Haahr, N Borgermann, X Guo, D Typas, D Achuthanku, S Hoffmann, R Shearer, TK Sixma, N MailandMol. Cell, 2018;0(0):.Species: HumanSample Types: Cell LysatesApplications: Bioassay
- Identification Of Small Molecule TRABID Deubiquitinase Inhibitors ByComputation-Based Virtual Screen.Authors: Shi T, Bao J, Wang N, Zheng J, Wu DBMC Chem Biol, 2012;12(1):4.Species: N/ASample Types: Recombinant ProteinApplications: Bioassay
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请问各位大神，做注射液中试时所用到磁力搅拌器需要资质吗？
加入溶液把搅拌子放在烧杯溶液中，将控温探头固定在橡胶夹头上，加热头插入至溶液中，不能影响到搅拌子，然后先插上仪器接插的电源插头，再接通电源打开电源调速开关，指标灯亮，即开始工作。
调速是由低速逐步调至高速，不允许高速档直接起动，以免搅拌子不同步，引起跳动。加热有开关控制，打开加热源开关，选择所需的温度，绿灯亮表明加热盘工作，红灯亮表明停止加热盘工作，此时进入恒温状态，不工作时应切断电源，为确保安全，使用时请接上地线．仪器应保持清洁干燥，严禁溶液进入机内，以免损坏机件防止剧烈震动．向左转|向右转
裂解液配方：
尿素7M
硫脲2M
CHAPS4%
二次水至20ml
使用前加DTT至65mM，tris至40mM，PMSF至1mM
上样液配方：
尿素8M
CHAPS2%
1%溴酚蓝0.002%
二次水至10ml
使用前加DTT至18mM，IPGbuffer至0.5%
由于都含有尿素，特别难溶解，好像含有尿素的温度不能超过37度，就在磁力搅拌器上一直搅着，发现过了一两个小时还没溶解，怎么回事么？中间还在34度左右水浴锅里水浴过，还是没溶解，溅在烧杯壁上的液体都已经结晶了。
有人碰到这样的问题么，很着急，急求帮助。
1.裂解液和上样液配方有无问题？
2.尿素怎么老不容，有没有什么方法加速其溶解？
本人想买一台制备胶体金的加热磁力搅拌器，大家帮忙推荐下什么品牌的较好，谢谢
鸣谢！
