| Product Name | Calpain Substrate, Fluorogenic(Z - AA)2Rh110 |
| Size | 5 mg |
| Catalog # | AS-60320-5 |
| US$ | $118 |
A very sensitive fluorogenic calpain substrate, Abs/Em=501/527 nm. In general, Rh110-based protease substrates are much more sensitive than the analogous AMC or AFC-based substrate. This enhanced sensitivity might be attributed to the greater fluorescence of the enzymatic product. The colorless and nonfluorescent (Z-Ala-Ala)2Rh110 is hydrolyzed to the highly fluorescent Rhodamine 110, which exhibits excellent spectral properties that match the optimal detection window of most fluorescence instruments. Alternatively, (Z-Ala-Ala)2Rh110 can also be used to detect calpain in a chromogenic mode since the enzymatic product (Rhodamine 110) exhibits a large extinction coefficient (close to 100,000 cm-1mol-1). After cleavage, Abs(max) is at 497 nm and Em(max) is at 527 nm. | |
| Detailed Information | |
| Storage | -20°C desiccated and protected from light |
| References | Gitler D and Spira ME (1998). Real time imaging of calcium-induced localized proteolytic activity after axotomy and its relation to growth cone formation. Neuron 20, 1123-35, Rothe G, et al. (1992).Flow cytometric analysis of protease activities in vital cells. Biol Chem Hoppe Seyler 373, 547-54, Rothe G, et al. (1992).Flow cytometric analysis of protease activities in vital cells. Biol Chem Hoppe Seyler 373, 547-54. |
| Molecular Weight | 885.6 |
| (Z-AA)2Rh110 | |
| Sequence(Three-Letter Code) | (Z - Ala - Ala)2Rh110 |
| Spectral Properties | Abs/Em = 497/520 nm |
| Solvent System | DMSO |
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请问各位大神,做注射液中试时所用到磁力搅拌器需要资质吗?
加入溶液把搅拌子放在烧杯溶液中,将控温探头固定在橡胶夹头上,加热头插入至溶液中,不能影响到搅拌子,然后先插上仪器接插的电源插头,再接通电源打开电源调速开关,指标灯亮,即开始工作。
调速是由低速逐步调至高速,不允许高速档直接起动,以免搅拌子不同步,引起跳动。加热有开关控制,打开加热源开关,选择所需的温度,绿灯亮表明加热盘工作,红灯亮表明停止加热盘工作,此时进入恒温状态,不工作时应切断电源,为确保安全,使用时请接上地线.仪器应保持清洁干燥,严禁溶液进入机内,以免损坏机件防止剧烈震动.向左转|向右转
裂解液配方:
尿素7M
硫脲2M
CHAPS4%
二次水至20ml
使用前加DTT至65mM,tris至40mM,PMSF至1mM
上样液配方:
尿素8M
CHAPS2%
1%溴酚蓝0.002%
二次水至10ml
使用前加DTT至18mM,IPGbuffer至0.5%
由于都含有尿素,特别难溶解,好像含有尿素的温度不能超过37度,就在磁力搅拌器上一直搅着,发现过了一两个小时还没溶解,怎么回事么?中间还在34度左右水浴锅里水浴过,还是没溶解,溅在烧杯壁上的液体都已经结晶了。
有人碰到这样的问题么,很着急,急求帮助。
1.裂解液和上样液配方有无问题?
2.尿素怎么老不容,有没有什么方法加速其溶解?
本人想买一台制备胶体金的加热磁力搅拌器,大家帮忙推荐下什么品牌的较好,谢谢
鸣谢!

