
Product Name | Calpain Substrate, Fluorogenic(Z - AA)2Rh110 |
Size | 5 mg |
Catalog # | AS-60320-5 |
US$ | $118 |
A very sensitive fluorogenic calpain substrate, Abs/Em=501/527 nm. In general, Rh110-based protease substrates are much more sensitive than the analogous AMC or AFC-based substrate. This enhanced sensitivity might be attributed to the greater fluorescence of the enzymatic product. The colorless and nonfluorescent (Z-Ala-Ala)2Rh110 is hydrolyzed to the highly fluorescent Rhodamine 110, which exhibits excellent spectral properties that match the optimal detection window of most fluorescence instruments. Alternatively, (Z-Ala-Ala)2Rh110 can also be used to detect calpain in a chromogenic mode since the enzymatic product (Rhodamine 110) exhibits a large extinction coefficient (close to 100,000 cm-1mol-1). After cleavage, Abs(max) is at 497 nm and Em(max) is at 527 nm. | |
Detailed Information | ![]() |
Storage | -20°C desiccated and protected from light |
References | Gitler D and Spira ME (1998). Real time imaging of calcium-induced localized proteolytic activity after axotomy and its relation to growth cone formation. Neuron 20, 1123-35, Rothe G, et al. (1992).Flow cytometric analysis of protease activities in vital cells. Biol Chem Hoppe Seyler 373, 547-54, Rothe G, et al. (1992).Flow cytometric analysis of protease activities in vital cells. Biol Chem Hoppe Seyler 373, 547-54. |
Molecular Weight | 885.6 |
(Z-AA)2Rh110 | |
Sequence(Three-Letter Code) | (Z - Ala - Ala)2Rh110 |
Spectral Properties | Abs/Em = 497/520 nm |
Solvent System | DMSO |
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请问各位大神,做注射液中试时所用到磁力搅拌器需要资质吗?
裂解液配方:
尿素7M
硫脲2M
CHAPS4%
二次水至20ml
使用前加DTT至65mM,tris至40mM,PMSF至1mM
上样液配方:
尿素8M
CHAPS2%
1%溴酚蓝0.002%
二次水至10ml
使用前加DTT至18mM,IPGbuffer至0.5%
由于都含有尿素,特别难溶解,好像含有尿素的温度不能超过37度,就在磁力搅拌器上一直搅着,发现过了一两个小时还没溶解,怎么回事么?中间还在34度左右水浴锅里水浴过,还是没溶解,溅在烧杯壁上的液体都已经结晶了。
有人碰到这样的问题么,很着急,急求帮助。
1.裂解液和上样液配方有无问题?
2.尿素怎么老不容,有没有什么方法加速其溶解?
鸣谢!
产品名称:磁力搅拌器ST5ST10
产品品牌:韩国FINEPCR
产品类别:磁力搅拌器
产品详细参数
外形小巧,性能卓越
不带加热的多点磁力搅拌器
高品质的无碳刷电机可提供安静而强有力的搅拌
搅拌转速从0—1300rpm可调
不锈钢表台可防止液体喷溅
外观小巧,操作安全简便
性能参数:
型号
ST5
ST10
转速范围
0—1300rpm
搅拌点间距离
90mm
容量
单点搅拌:2L
250ml*5
250ml*10
材质
表台:不锈钢
重量
3Kg
5Kg
尺寸大小(W*D*H)
114*564*63mm
197*564*63mm
功率
15W
30W
电源
110V/220V/230V,50/60Hz
更多详情请登录:http://www.sci-bridge.com/ProductsList.aspx?pid=186
谢谢!
不知道有没有卖适合我用的搅拌棒,有的话买一个也行!
在园里查找有一个hbchendl战友提供的方法:
用钳子把小铁钉的两头给剪下来,毛滴管切割成适当的长度,短棒可用细的一头,长棒就用粗的部分,将铁钉塞进毛滴管中,再将两头用酒精灯火给烧死,就可以用了。
0.5cm的得用细铁钉,3cm的搅拌棒得用粗一点的铁钉。
对于这个方法,有一个问题,什么是毛滴管,用什么切割?谢谢

