Rabbit polyclonal IgG purified by Protein A affinity chromatography
Immunogen: Recombinant full-length human SPARC expressed in human cells
Specificity: Human, mouse, rat (not tested for other species)
Application: Western blot 2-10 ug/mL,ELISA 0.1-1 Ug/mL.
Not tested for other applications.
Preservative: None
Sterility: 0.2 um membrane-filtered and packaged aseptically.
QC tests: SDS-PAGE, Western blotting, Direct ELISA
Formulation: Lyophilized from a sterile filtered solution of phosphate-buffered saline (PBS)
Reconstitution: Add sterile distilled water to a concentration of 1 mg/mL. Further dilutions should be made in appropriate buffers.
Storage and stability: Upon receiving, store the product at -20°C. After reconstitution, store the working aliquots at 2-8°C for up to 2 weeks. For extended storage, aliquot the solution and freeze at -70°C or -20°C. Avoid repeated freezing and thawing.
About SPARC: SPARC (secreted protein, acidic and rich in cysteine) is a matrix associated protein that elicits changes in cell shape, inhibits cell cycle progression, and influences the synthesis of extracellular matrix (ECM). It is also known as osteonectin (ON) or basement-membrane protein 40 (BM-40). SPARC contains two calcium binding sites; an N-terminal acidic region and a C-terminal extracellular calcium binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis.
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我们实验室比较简陋，没有超声破碎仪，能做原核表达吗？用溶菌酶代替可以吗
可储存十套常规程序数据和一套组合程序，工作方式有定时和计数两种。
组合程序最多可以由十套常规程序组成，可选择循环或不循环工作模式。
超温保护及报警功能。
超声波输出强度自动限定功能。
定时方式工作时间定时：0～99小时59分59秒。
计数方式超声工作次数：0～9999次。
超声时间范围：0～99小时59分59秒。
间隙时间范围：0～99小时59分59秒。间隙时间=0为超声连续工作。
温度控制精度：±1℃。
工作电压：VAC100-240，50-60Hz。
工作环境：室内（无潮湿，无阳光直射，无腐蚀性气体向左转|向右转
●高能效换能器
●振幅自动调节，在不同的负载状况时振幅保持一致
●设置超声间歇时间
●微机控制,超声功率连续调节
●集成温度控制样品温度
●隔音箱均采用特殊隔音材料向左转|向右转
超声破碎的条件不能一概而论，要看你的实验要求，有的是细菌破碎，有的是对组织细胞进行破碎，要掌握好功率和每次超声时间，功率大时，每次超声时间可缩短，不能让温度升高，必要时在冰浴条件下进行超声破碎。至于每次超声时是否起泡沫到不是关键的问题，这与你超声的量明显有关。
