PRODUCT SPECIFICATION
Product# 1121: Murine anti-gp120 HIV-1 Monoclonal Antibody
(Cross Reactive)
SPECIFICATIONS APPLICATIONS
Antibody Class: IgG2b ELISA, Western ELISA
Concentration: See vial Immunoprecipitation
Mass/vial: 100µg Immunocytochemistry
Purity: >95% Immunohistochemistry
Stabilizer: None In Vitro Virus Neutralization
Preservative: None
Physical State: Frozen Liquid
Storage: -75oC
Stability: At least 12 months at -75oC
DESCRIPTION: High affinity murine anti-gp120 HIV-1 mAb. Epitope: 315 to 330 amino acids of HIV-1 gp120.
SPECIFICITY: Binds to V3-loop of HIV-1 gp120/gp160. Cross reactive with MN, IIIB, RF, SF2 and other primary HIV-1 isolates.
BIOLOGICAL ACTIVITY: Neutralizes different strains of HIV-1 at 1-10µg/ml as determined by syncycium assays with CD4-bearing HIV-1 infected cells and co-cultures of HIV-1 infected human PBLs.
APPLICATIONS AND INSTRUCTIONS FOR USE
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
ELISA may be performed at 10-100ng/ml of mAb depending on the amount of immobilized antigen in the assays. Western ELISA require at 1-5µg/ml mAb..
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溶解后加培养液稀释不会出现悬浮浑浊吗？？？？？？
二是镜检染色观察计数，破碎完全的基本看不到完整的菌体，都是细胞碎片
三是测菌液的电导率，根据电导率大小来判断。
顺便问下宁波新芝这个牌子的超声波破碎仪质量如何其他方面如何谢谢
