
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
---|---|---|---|---|---|---|
Plasmid | 124603 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | Add to Cart | |
AAV9 | 124603-AAV9 | Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information | Add to Cart |
This material is available to academics and nonprofits only.
Backbone
- Vector backbonepcDNA(Search Vector Database)
- Backbone manufacturerGenscript
- Backbone sizew/o insert(bp)3000
- Total vector size (bp)7301
- Vector typeMammalian Expression, AAV
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)NEB Stable
- Copy numberHigh Copy
Gene/Insert
- Gene/Insert nameSoma-localized ChrimsonR
- SpeciesM. musculus (mouse)
- Insert Size (bp)3385
- Tag/ Fusion Protein
- mRuby2 (C terminal on insert)
Resource Information
- Supplemental Documents
- pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV21TS-WPRE-SV40.gbk
- A portion of this plasmid was derived from a plasmid made byGenscript
- Terms and Licenses
- UBMTA
- genOway Notice of RIghts
- Industry Terms
- Not Available to Industry
Information for AAV9 (Catalog # 124603-AAV9)(Back to top)
Purpose
Ready-to-use AAV9 particles produced from pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 (#124603). In addition to the viral particles, you will also receive purified pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 plasmid DNA.
EF1a-driven, Cre-dependent expression of ChrimsonR fused to mRuby2 fluorophore and targeted to neuronal soma for optogenetic activation. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 1×10¹³ vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV9 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV9
- PurificationIodixanol gradient ultracentrifugation
- Reporter GenemRuby2 (Cre-dependent)
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Addgene Comments
Using FLEX vectors in vivo: LoxP sites in FLEX plasmids are known to recombine during DNA amplification and viral vector production, which may result in a minority of Cre-activated (i.e., "flipped") viral vectors. Addgene has measured this occurs in 0.01-0.03% of viral vectors in our typical production protocol. This can lead to a small number of cells exhibiting Cre-independent transgene expression in vivo. To address this, we recommend titrating to find the optimal AAV dosage required for Cre-dependent transgene expression and function in vivo. This may include reducing the viral vector dosage in order to reduce the likelihood of Cre-independent expression.
ebiomall.com






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可储存十套常规程序数据和一套组合程序,工作方式有定时和计数两种。
组合程序最多可以由十套常规程序组成,可选择循环或不循环工作模式。
超温保护及报警功能。
超声波输出强度自动限定功能。
定时方式工作时间定时:0~99小时59分59秒。
计数方式超声工作次数:0~9999次。
超声时间范围:0~99小时59分59秒。
间隙时间范围:0~99小时59分59秒。间隙时间=0为超声连续工作。
温度控制精度:±1℃。
工作电压:VAC100-240,50-60Hz。
工作环境:室内(无潮湿,无阳光直射,无腐蚀性气体向左转|向右转
●高能效换能器
●振幅自动调节,在不同的负载状况时振幅保持一致
●设置超声间歇时间
●微机控制,超声功率连续调节
●集成温度控制样品温度
●隔音箱均采用特殊隔音材料向左转|向右转
我们实验室比较简陋,没有超声破碎仪,能做原核表达吗?用溶菌酶代替可以吗

