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Description
Data
siRNA Design
Citations
- High siRNA transfection efficiency
- Functional gene silencing post siRNA delivery
- Compatibility with diverse growth conditions (with and without serum)
- Low cytotoxicity
- Easy-to-use protocols for both adherent and suspension cells
Contents
- Hydrated GeneSilencer Lipid
- siRNA Diluent (corresponding ratio for each kit)
Storage
GeneSilencer siRNA Transfection Reagent is shipped at room temperature, as similar to other gene therapy products. For maximum stability, store all reagents at 4篊 upon receipt. If stored properly, all components are stable for 6 months.GeneSilencer Transfection Reagent Kit
- 50 rxn kit, 0.18 ml, T500020
- 200 rxn kit, 0.75 ml, T500750
- 1000 rxn kit, 5 x 0.75 ml, T505750
- 10 rxn sample kit, 0.03 ml, T500020S
Long Noncoding RNA pancEts-1 Promotes Neuroblastoma Progression through hnRNPK-Mediated β-Catenin Stabilization.Li, D.,et al (2018) Cancer Res. 78 (5): 1169-1183. Link
Apoptotic cells trigger the ABCA1/STAT6 pathway leading to PPAR‐γ expression and activation in macrophages.Kim, M-J.,et al (2018) J. Leukocyte Biol. 103(5): 885-895. Link
Transient Silencing of DNA Repair Genes Improves Targeted Gene Integration in the Filamentous Fungus Trichoderma reesei.Chum, P.Y.,et al ((2017) Appl. Environ. Microbiol. 83(15): e00535-17. Link
Programming of macrophages by apoptotic cancer cells inhibits cancer progression through exosomal PTEN and PPARγ ligands.Kim, Y-B.,et al (2017) bioRxiv 217562: 1-75. Link
Role of Sphingosine Kinase 1 and S1P Transporter Spns2 in HGF-mediated Lamellipodia Formation in Lung Endothelium.Fu, P.,et al (2016) J. Biol. Chem. 291(53): 27187?27203. Link
Ubiquitin-specific Protease 20 Regulates the Reciprocal Functions of β-Arrestin2 in Toll-like Receptor 4-promoted Nuclear Factor κB (NFκB) Activation.Jean-Charles, P-Y., (2016) J. Biol. Chem. 291 (14): 7450 ?7464. Link
Tolerogenic nanoparticles inhibit T cell杕ediated autoimmunity through SOCS2.Yeste, A.,et al (2016) Sci. Signal. 9(433): RA61. Link
Synthesis of an Endogenous Steroidal Na Pump Inhibitor Marinobufagenin, Implicated in Human Cardiovascular Diseases, Is Initiated by CYP27A1 via Bile Acid Pathway.Fedorova, O.V.,et al (2015) Circ Cardiovasc Genet. 8(5): 736-45. Link
Thioredoxin Activates MKK4-NFκB Pathway in a Redox-dependent Manner to Control Manganese Superoxide Dismutase Gene Expression in Endothelial Cells.Kundumani-Sridharan, V.,et al (2015) J. Biol. Chem. 290(28): 17505?17519. Link
Identification, Mechanism of Action, and Antitumor Activity of a Small Molecule Inhibitor of Hippo, TGF-β, and Wnt Signaling Pathways.Basu, D.,et al (2014) Mol. Cancer Ther. 13(6): 1457-1467. Link
PKC Potentiates Tyrosine Kinase Inhibitors STI571 and Dasatinib Cytotoxic Effect.Tob蚾, A.,et al (2014) Anticancer Res. 34(7): 3347-3356. Link
TREK2 Expressed Selectively in IB4-Binding C-Fiber Nociceptors Hyperpolarizes Their Membrane Potentials and Limits Spontaneous Pain.Acosta, C.,et al (2014) J. Neurosci. 34(4): 1494-1509. Link
RhoA/Phosphatidylinositol 3-Kinase/Protein Kinase B/Mitogen-Activated Protein Kinase Signaling after Growth Arrest朣pecific Protein 6/Mer Receptor Tyrosine Kinase Engagement Promotes Epithelial Cell Growth and Wound Repair via Upregulation of Hepatocyte Growth Factor in Macrophages.Lee, Y-J.,et al (2014) J. Pharma. Exp. Thera. 350(3): 563-577. Link
GeneSilencer Cell Line Citations: 3T3-L1, A549, A7, AGS, ASM Transformed, B16-F1, BEL7404, Bronchial C166, C2C12, C6R, CHO-K1, DC2.4, GS-KB-3-1, H22, H441, H460, H9c2, hCMEC/D3, HCP40, HCT-116, HEK 293, HEK 293T, HEK-293, HeLa, HepG2, HL-60, HMEC-1, HPAEC, HT29, HUT-78, JAR, Jurkat, KATOIII, L/Stab-2, LNCaP, M2, McA-RH 7777, MCF-7, MDA MB-231, MDA MB-468, MDA-MB-435, MG63, MKN 28, MKN 45, MV-4-11, Neuro2a, NIH-3T3, OK, PC-12, Cortical Neurons, Hippocampal Neurons, Macrophage, Pulmonary Artery, Endothelium, Dendritic Cells(Bone Marrow), Cortical Neurons, Coronary Artery Endothelial, Peritoneal Macrophage, Dorsal Root Ganglion Neurons, Retinal Ganglion, Dorsal Root Ganglion, Schwann Cells Cardiac Fibroblast, Cardiac Myocytes (Neonatal), Lung Microvascular Endothelial Cells, Aortic Muscle Cells, Dendritic Cells, Cerebellar Neurons, Osteoblast, HMVEC, Cardiac Myocytes, T-Cells (CD3+), Cortical Neurons, Dendritic Cells, Cortical Neurons, Aveolar Type II Cells, Macrophage, Dorsal Root Ganglion Lacrimal Gland Acinar Cells, Bone Marrow, Dendritic Cells, Pulmonary Artery Endothelium, Primary HPAEC. RAW 264.7, Renca, SaOs2, Saos-2, SCCH196, SNB19, SW480, U20S
GeneSilencerTM Reagent vs. siPORTTM NeoFXTM Reagent
HEK 293 Cells (click on images to enlarge)
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Figure Legend: HEK 293 cells were transfected with 5, 10, 30 or 50 nM of Silencer FAM Labeled GAPDH siRNA with either GeneSilencer (Genlantis) or siPORT NeoFX (Ambion) siRNA transfection reagents according to the manufacturer"s recommended protocols. Cells were incubated for 48 hours then fixed, permeablized, and incubated with AlexaFluor 555 phalloidin (Invitrogen), which stains cellular actin red. Cells were then mounted on slides and stained with DAPI (Invitrogen), which stains the cell nuclei blue. Transfected cells were visualized by fluorescence microscopy using identical exposure times for FITC, TRITC, and DAPI. The transfected GAPDH siRNA is localized in the cell cytosol and can be seen as green fluorescent specks or dots.
HeLa S3 Cells (click on images to enlarge)
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Figure Legend: HeLa S3 cells were transfected with 5, 10, 30 or 50 nM of Silencer FAM labeled GAPDH siRNA with either GeneSilencer (Genlantis) or siPORT&tm; NeoFX&tm; (Ambion) siRNA transfection reagents according to the manufacturer"s recommended protocols. Cells were incubated for 48 hours then fixed, permeablized, and incubated with AlexaFluor 555 phalloidin (Invitrogen), which stains cellular actin red. Cells were then mounted on slides and stained with DAPI (Invitrogen), which stains the cell nuclei blue. Transfected cells were visualized by fluorescence microscopy using identical exposure times for FITC, TRITC, and DAPI. The transfected GAPDH siRNA is localized in the cell cytosol and can be seen as green fluorescent specks or dots.
How to Design siRNA (Small Interfering RNA)
The sequence of the siRNA can be selected as follows:
The sequence selection process has no other constraints. It is important to note that structure within thetargeted mRNA appears to have minimal effect on the availability of the mRNA target and efficacy of thesiRNA silencing approach. To-date, successful silencing has been achieved using the above method toselect the target sequence, although the method is essentially random with respect to accounting for mRNAstructure.Although siRNA silencing appears to be extremely effective by selecting a single target in the mRNA, itmay be desirable to design and employ two independent siRNA duplexes to control for specificity of thesilencing effect. This recommendation is only for specificity for it is yet unknown if the targeting of a geneby two different siRNA duplexes would be more effective than using a single siRNA duplex. It is believedthat the rate-limiting component of the siRNA effect is the availability of cellular nuclease components andnot mRNA target availability. Therefore, doubling the number of siRNA duplexes is not expected to doublethe rate or efficiency of silencing.If the selected siRNA duplex(es) do not function for silencing, the following steps are recommended. First,a search is conducted for sequencing errors in the gene and possible polymorphisms. Initial studies on thespecificity of target recognition by siRNA duplexes indicates that a single point mutation located in thepaired region of an siRNA duplex is sufficient to abolish target mRNA degradation. Second, a reexaminationis performed to confirm whether the cell line is from the expected species. Third, a secondand/or third target are selected and the corresponding siRNA duplexes prepared.
| Cell Line | Cell Type | Source | Genlantis Product | Citation |
| 3T3-L1 | Embryo | Swiss Mouse | GeneSilencer siRNA Transfection Reagent | Mizuarai, S., Miki, S., Araki, H., Takahashi, K. and Kotani, H.(2005) Identification of Dicarboxylate Carrier Slc25a10 as Malate Transporter in de Novo Fatty Acid Synthesis. J. Biol. Chem.280(37): p. 32434-32441. |
| A549 | Lung Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Ito, K., Yamamura, S., Essilfie-Quaye, S., Cosio, B., Ito, M., Barnes, P.J. and Adcock, I.M. (2006) Histone deacetylase 2-mediated deacetylation of the glucocorticoid receptor enables NFκB suppression. J. Exp. Med. 203(1): 7-13. |
| A549 | Lung Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Stepulak, A., Sifringer, M., Rzeski, W., Endesfelder, S., Gratopp, A., Pohl, E.E., Bittigau, P., Hansen, H.H., Stryjecka-Zimmer, M., Turski, L. and Ikonomidou, C. (2005) NMDA antagonist inhibits the extracellular signal-regulated kinase pathway and suppresses cancer growth. Proc. Natl. Acad. Sci. 102: 15605 - 15610. |
| A7 | Melanoma | Human | GeneSilencer siRNA Transfection Reagent | Scott, M.G.H., Pierotti, V., Storez, H., Lindberg, E., Thuret, A., Muntaner, O., Labbe-Jullie, C., Pitcher, J.A. and Marullo, S. (2006) Cooperative Regulation of Extracellular Signal-Regulated Kinase Activation and Cell Shape Change by Filamin A and β-Arrestins. Mol. & Cell Biol, 26: 3432-3445. |
| AGS | Gastric Epithelium | Human | GeneSilencer siRNA Transfection Reagent | Nagasako, T., Sugiyama, T., Mizushima, T., Miura, Y., Kato, M. and Asaka, M. (2003) Up-regulated Smad5 Mediates Apoptosis of Gastric Epithelial Cells Induced by Helicobacter pylori Infection. J. Biol. Chem.: 278: 4821 - 4825. |
| AGS | Gastric Epithelium | Human | GeneSilencer siRNA Transfection Reagent | Varro, A., Noble, P-J. M., Pritchard, D.M., Kennedy, S., Hart, C.A., Dimaline, R. and Dockray, G.J. (2004) Helicobacter pylori Induces Plasminogen Activator Inhibitor 2 in Gastric Epithelial Cells through Nuclear Factor-B and RhoA: Implications for Invasion and Apoptosis Cancer Res. 64: 1695 - 1702. |
| ASM Transformed | Airway Smooth Muscle Cells | Human | GeneSilencer siRNA Transfection Reagent | Tran, T., Ens-Blackie, K., Rector, E.S., Stelmack, G.L., McNeill, K.D., Tarone, G., Gerthoffer, W.T., Unruh, H. and Halayko, A.J. (2007) Laminin-binding Integrin α7 is Required for Contractile Phenotype Expression by Human Airway Myocyte. Am. J. Respir. Cell Mol. Biol. 37(6): 668-80. |
| B16-F1 | Melanoma | Mouse | GeneSilencer siRNA Transfection Reagent | Bertling, E., Hotulainen, P., Mattila, P.K., Matilainen, T., Salminen, M., and Lappalainen, P. (2004) Cyclase-associated-protein 1 (CAP1) promotes cofilin-induced actin dynamics in mammalian nonmuscle cells. Mol. Biol. Cell 15 (5): p. 2324-2334. |
| B16-F1 | Melanoma | Mouse | GeneSilencer siRNA Transfection Reagent | Hotulainen, P., Paunola, E., Vartiainen, M.K. and Lappalainen, P. (2005) Actin-depolymerizing Factor and Cofilin-1 Play Overlapping Roles in Promoting Rapid F-Actin Depolymerization in Mammalian Nonmuscle Cells. Mol. Biol. Cell. 16(2): p. 649-664. |
| BEL7404 | Hepatoma | Human | GeneSilencer siRNA Transfection Reagent | Liang, X-J, Finkel, T., Shen, D-W, Yin, J-J, Aszalos, A. and Gottesman, M.M. (2008) SIRT1 Contributes in Part to Cisplatin Resistance in Cancer Cells by Altering Mitochondrial Metabolism. Mol Cancer Res 6(9): 1499-506. |
| Bronchial | Smooth Muscle | Human | GeneSilencer siRNA Transfection Reagent | Nunes, R.O., Schmidt, M., Dueck, G., Baarsma, H., Halayko, A.J., Kerstjens, H.A.M., Meurs, H. and Gosens, R. (2008) GSK-3/β-catenin signaling axis in airway smooth muscle: role in mitogenic signaling Am J Physiol Lung Cell Mol Physiol. 294: L1110 - L1118. |
| C166 | Yolk Sack Endothelium | Mouse | GeneSilencer siRNA Transfection Reagent | Zhou, X., Stuart, A., Dettin, L.E., Rodriguez, G., Hoel, B. and Gallicano G.I. (2004) Desmoplakin is required for microvascular tube formation in culture. J. Cell Sci. 117: 3129-3140. |
| C2C12 | Myoblast | Mouse | GeneSilencer siRNA Transfection Reagent | Evangelisti, C., Tazzari, P.L., Riccio, M., Fiume, R., Hozumi, Y., Fala, F., Goto, K.., Manzoli, L., Cocco, L. and Martelli, A.M. (2007) Nuclear diacylglycerol kinase-ζ is a negative regulator of cell cycle progression in C2C12 mouse myoblasts. FASEB J. 21: 3297 - 3307 |
| C6R | Glioma | Rat | GeneSilencer siRNA Transfection Reagent | Saarikangas, J., Hakanen, J., Mattila, P.K., Grumet, M., Salminen, M. and Lappalainen, P. (2008) ABBA regulates plasma-membrane and actin dynamics to promote radial glia extension. J Cell Sci. 121(Pt 9):1444-54. |
| CHO-K1 | Ovary | Chinese Hamster | GeneSilencer siRNA Transfection Reagent | Kheifets, V., Bright, R., Inagaki, K., Schechtman, D. and Mochly-Rosen, D. (2006) Protein Kinase C δ PKC-Annexin V Interaction: a required step in δ PKC translocation and function. J. Biol. Chem. 281(32): p. 23218-23226. |
| DC2.4 | Bone Marrow | Mouse | GeneSilencer siRNA Transfection Reagent | Jing, H., Yen, J-H and Ganea, D. (2004) A novel signaling pathway mediates the inhibition of CCL3/4 expression by PGE2. J. Biol. Chem. 279(53): p. 55176-55186. |
| GS-KB-3-1 | Epidermoid Adenocarcinoma | Human | GeneSilencer siRNA Transfection Reagent | Liang, X-J, Finkel, T., Shen, D-W, Yin, J-J, Aszalos, A. and Gottesman, M.M. (2008) SIRT1 Contributes in Part to Cisplatin Resistance in Cancer Cells by Altering Mitochondrial Metabolism. Mol Cancer Res 6(9): 1499-506. |
| H22 | Hepatoma | Mouse | GeneSilencer siRNA Transfection Reagent | Huang, B., Lei, Z., Zhang, G-M, Li, D., Song, C., Li, B., Liu, Y., Yuan, Y., Unkeless, J., Xiong, H. and Feng, Z-H (2008) SCF-mediated mast cell infiltration and activation exacerbate the inflammation and immunosuppression in tumor microenvironment. Blood 112(4): 1269-79. |
| H22 | Hepatoma | Mouse | GeneSilencer siRNA Transfection Reagent | Huang, B., Zhao, J., Shen, S., Li, H., He, K-L, Shen, G-X, Mayer, L., Unkeless, J., Li, D., Yuan, Y., Zhang, G.-M., Xiong, H. and Feng, Z.-H. (2007) Listeria monocytogenes Promotes Tumor Growth via Tumor Cell Toll-Like Receptor 2 Signaling. Cancer Res. 67(9): p. 4346-4352. |
| H441 | Lung Adenocarcinoma | Human | GeneSilencer siRNA Transfection Reagent | Zhang, Y-A., Nemunaitis, J., Samuel, S.K., Chen, P., Shen, Y. and Tong, A.W. (2006) Antitumor Activity of an Oncolytic Adenovirus-Delivered Oncogene Small Interfering RNA. Cancer Res. 66(19): 9736-9743. |
| H460 | Lung Cancer | Human | GeneSilencer siRNA Transfection Reagent | Ren, J., Shi, M., Liu, R., Yang, Q-H., Johnson, T., Skarnes, W.C. and Du, C. (2005) The Birc6 (Bruce) gene regulates p53 and the mitochondrial pathway of apoptosis and is essential for mouse embryonic development. Proc. Natl. Acad. Sci. USA. 102(3): p. 565-570. |
| H9c2 | Myoblast | Rat | GeneSilencer siRNA Transfection Reagent | Saotome, M., Safiulina, D., Szabadkai, G., Das, S., Fransson, A., Aspenstrom, P., Rizzuto, R. and Hajnoczky, G. (2008) Bidirectional Ca2+-dependent control of mitochondrial dynamics by the Miro GTPase. Proc Natl Acad Sci U S A 105(52): p. 20728. |
| hCMEC/D3 | Brain Microvascular Endothelia | Human | GeneSilencer siRNA Transfection Reagent | Huang, W., Eum, S.Y., Andras, I.E., Hennig, B. and Toborek, M. (2009) PPAR{alpha} and PPAR{gamma} attenuate HIV-induced dysregulation of tight junction proteins by modulations of matrix metalloproteinase and proteasome activities. FASEB J 13 Jan 2009. |
| HCP40 | Colon adenocarcinoma | null | GeneSilencer siRNA Transfection Reagent | Yao, K., Shida, S., Selvakumaran, M., Zimmerman, R., Simon, E., Schick, J., Haas, N.B., Balke, M., Ross, H., Johnson, S.W. and O"Dwyer, P.J. (2005) Macrophage Migration Inhibitory Factor Is a Determinant of Hypoxia-Induced Apoptosis in Colon Cancer Cell Lines. Clin Cancer Res. 11 (20): 7264 - 7272. |
| HCT-116 | Colon Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Wang, J., Rajput, A., Kan, J.L.C., Rose, R., Liu, X., Kuropatwinski, K., Hauser, J., Beko, A., Dominquez, I., Sharratt, E.A., Brattain, L., LeVea, C., Sun, F., Keane, D.M., Gibson, N.W. and Michael G. Brattain (2009) Knockdown of Ron kinase inhibits mutant PI3 kinase and reduces metastasis in human colon carcinoma. J. Biol. Chem. 284 (16) 10912-10922. |
| HCT-116 | Colon Adenocarcinoma | Human | GeneSilencer siRNA Transfection Reagent | Yao, K., Shida, S., Selvakumaran, M., Zimmerman, R., Simon, E., Schick, J., Haas, N.B. Balke, M., Ross, H., Johnson, S.W. and O"Dwyer, P.J. (2005) Macrophage Migration Inhibitory Factor Is a Determinant of Hypoxia-Induced Apoptosis in Colon Cancer Cell Lines. Clin. Can. Res. 11: 7264-7272. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Ahn, S., Nelson, C.D., Garrison, T.R., Miller, W.E. and Lefkowitz, R.J. (2003) Desensitization, internalization, and signaling functions of -arrestins demonstrated by RNA interference. Proc. Natl. Acad. Sci. 100: 1740 - 1744. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Ahn, S., Shenoy, S.K., Wei, H. and Lefkowitz, R.J. (2004) Differential Kinetic and Spatial Patterns of {beta}-Arrestin and G Protein-mediated ERK Activation by the Angiotensin II Receptor. J. Biol. Chem. 279 (34): 35518-35525. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Ahn, S., Wei, H., Garrison, T.R. and Lefkowitz, R.J. (2004) Reciprocal Regulation of Angiotensin Receptor-activated Extracellular Signal-regulated Kinases by (beta)-Arrestins 1 and 2. J. Biol. Chem. 279: 7807-7811. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Khundmiri, S.J., Dean, W.L., McLeish, K.R. and Lederer, E.D. (2005) Parathyroid Hormone-mediated Regulation of Na+-K+-ATPase Requires ERK-dependent Translocation of Protein Kinase C{alpha}. J. Biol. Chem. 280(10): p. 8705-8713. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Kim, J., Ahn, S., Ren, X-R., Whalen, E.J., Reiter, E., Wei, H. and Lefkowitz, R.J. (2005) Functional antagonism of different G protein-coupled receptor kinases for {beta}-arrestin-mediated angiotensin II receptor signaling. Proc. Natl. Acad. Sci.驴102: 1442 - 1447. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Kohout, T.A., Nicholas, S.L., Perry, S.J., Reinhart, G., Junger, S. and Struthers, R. (2004) Differential Desensitization, Receptor Phosphorylation, {beta}-Arrestin Recruitment, and ERK1/2 Activation by the Two Endogenous Ligands for the CC Chemokine Receptor 7. J. Biol. Chem. 279 (22): p. 23214-23222. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Liang, Y., Yu, W., Li, Y., Yan, X., Huang, Q. and Zhu, X. (2004) Nudel functions in membrane traffic mainly through association with Lis1 and cytoplasmic dynein. J. Cell Biol. 164 (4): 557-566. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Ren, X-R., Reiter, E., Ahn, S., Kim, J., Chen, W. and Lefkowitz, R.J. (2005) Different G protein-coupled receptor kinases govern G protein and {beta}-arrestin-mediated signaling of V2 vasopressin receptor. Proc. Natl. Acad. Sci. 102: 1448 - 1453. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Shenoy, S.K., Drake, M.T., Nelson, C.D., Houtz, D.A., Xiao, K., Madabushi, S., Reiter, E., Premont, R.T., Lichtarge, O. and Lefkowitz, R.J. (2006) {beta}-Arrestin-dependent, G Protein-independent ERK1/2 Activation by the {beta}2 Adrenergic Receptor. J. Biol. Chem. 281(2): 1261-1273. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Shikama, Y., Yamada, M., Miyashita, T. (2004) Caspase-8 and caspase-10 activate NF-B through RIP, NIK and IKK kinases. Eur. J. Immun. 33 (7): 1998-2006. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Wei, H., Ahn, S., Barnes, W.G. and Lefkowitz, R.J., (2004) Stable interaction between beta -arrestin2 and AT 1A receptor is required for beta -arrestin2 mediated activation of extracellular signal-regulated kinase 1 and 2. J. Biol. Chem. 279: 48255-48261. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Wei, H., Ahn, S., Shenoy, S.K., Karnik, S.S., Hunyady, L., Luttrell, L.M. and Lefkowitz, R.J. (2003) Independent -arrestin 2 and G protein-mediated pathways for angiotensin II activation of extracellular signal-regulated kinases 1 and 2. Proc. Natl. Acad. Sci. 100: 10782 - 10787. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Wu, J-H, Goswami, R., Kim, L.K., Miller, W.E., Peppel, K. and Freedman, N.J. (2005) The Platelet-derived Growth Factor Receptor-{beta} Phosphorylates and Activates G Protein-coupled Receptor Kinase-2: a Mechanism for Feedback Inhibition. J. Biol. Chem. 280(35): p. 31027-31035. |
| HEK 293T | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Wei, H., Ahn, S., Shenoy, S.K., Karnik, S.S., Hunyady, L., Luttrell, L.M. and Lefkowitz, R.J. (2003) Independent -arrestin 2 and G protein-mediated pathways for angiotensin II activation of extracellular signal-regulated kinases 1 and 2. Proc. Natl. Acad. Sci. 100: 10782 - 10787. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Ahn, S., Kim, J., Hara, M.R., Ren, X. and Lefkowitz, R.J. (2009) β -arrestin2 mediates anti-apoptotic signaling through regulation of bad phosphorylation. J Biol Chem 284(13): 8855-65. |
| HEK-293 | Embryonic Fibroblast | Human | GeneSilencer siRNA Transfection Reagent | Barthet, G., Framery, B., Gaven, F., Pellissier, L., Reiter, E., Claeysen, S., Bockaert, J. and Dumuis, A. (2007) 5-Hydroxytryptamine 4 Receptor Activation of the Extracellular Signal-regulated Kinase Pathway Depends on Src Activation but Not on G Protein or beta-Arrestin Signaling. Mol. Biol. Cell. 18(6): 1979-1991. |
| HEk-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | El-Shewy, H.M., Johnson, K.R., Lee, M-H, Jaffa, A.A., Obeid, L.M. and Luttrell, L.M. (2006) Insulin-like Growth Factors Mediate Heterotrimeric G Protein-dependent ERK1/2 Activation by Transactivating Sphingosine 1-Phosphate Receptors . J. Biol. Chem. 281(42): 31399-31407. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | El-Shewy, H.M., Lee, M-H., Obeid, L.M., Jaffa, A.A. and Luttrell, L.M. (2007) The Insulin-like Growth Factor Type 1 and Insulin-like Growth Factor Type 2/Mannose-6-phosphate Receptors Independently Regulate ERK1/2 Activity in HEK293 Cells. J. Biol. Chem. 282(36): 26150-26157. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Gesty-Palmer, D., Chen, M., Reiter, E., Ahn, S., Nelson, C.D., Wang, S., Eckhardt, A.E., Cowan, C.L., Spurney, R.F., Luttrell, L.M. and Lefkowitz, R.J. (2006) Distinct beta-Arrestin- and G Protein-dependent Pathways for Parathyroid Hormone Receptor-stimulated ERK1/2 Activation. J. Biol. Chem. 281(16): 10856-10864. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | IM Kim, DG Tilley, J Chen, NC Salazar, EJ Whalen, JD Violin, and HA Rockman (2008) Beta-blockers alprenolol and carvedilol stimulate beta-arrestin-mediated EGFR transactivation. Proc Natl Acad Sci 105(38): 14555-60. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Kani, S., Nakayama, E., Yoda, A., Onishi, N., Sougawa, N., Hazaka, Y., Umeda, T., Takeda, K., Ichijo, H., Hamada, Y. and Minami, Y. (2007) Chk2 kinase is required for methylglyoxal-induced G2/M cell-cycle checkpoint arrest: implication of cell-cycle checkpoint regulation in diabetic oxidative stress signaling. Genes Cells. 12(8): 919-928. |
| HEk-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Kara, E., Crepieux, P., Gauthier, C., Martinat, N., Piketty, V., Guillou, F., and Reiter, E. (2006) A Phosphorylation Cluster of Five Serine and Threonine Residues in the C-Terminus of the Follicle-Stimulating Hormone Receptor Is Important for Desensitization But Not for β-Arrestin-Mediated ERK Activation. Mol. Endocrinol. 20(11): p. 3014-3026. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Nelson, C.D., Kovacs, J.J., Nobles, K.N., Whalen, E.J. and Lefkowitz, R.J. (2008) β-Arrestin Scaffolding of Phosphatidylinositol 4-Phosphate 5-Kinase Iα Promotes Agonist-stimulated Sequestration of the {beta}2-Adrenergic Receptor. J. Biol. Chem. 283(30): p. 21093-21101. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Noma, T., Lemaire, A., Prasad, S.V.N., Barki-Harrington, L., Tilley, D.G., Chen, J., Le Corvoisier, P., Violin, J.D., Wei, H., Lefkowitz, R.J. and Rockman, H.A. (2007) β-Arrestin mediated β1-adrenergic receptor transactivation of the EGFR confers cardioprotection. J. Clin. Invest. 117(9): p. 2445-2458. |
| HEk-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Rajagopal, K., Whalen, E.J., Violin, J.D., Stiber, J.A., Rosenberg, P.B., Premont, R.T., Coffman, T.M., Rockman, H.A. and Lefkowitz, R.J. (2006) β-Arrestin2-mediated inotropic effects of the angiotensin II type 1A receptor in isolated cardiac myocytes. PNAS. 103: 16284 - 16289. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Shenoy, S.K., Xiao, K., Venkataramanan, V., Snyder, P.M., Freedman, N.J. and Weissman, A.M. (2008) NEDD4 mediates agonist-dependent ubiquitination, lysosomal targeting and degradation of the beta 2 adrenergic receptor. J. Biol. Chem. 283: 22166 - 22176. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Violin, J.D., DeWire, S.M., Barnes, W.G. and Lefkowitz, R.J. (2006) G Protein-Coupled Receptor Kinase and β-arrestin mediated desensitization of the angiotensin II type 1A receptor elucidated by diacylgylcerol dynamics. J. Biol. Chem. 281: 36411 - 36419. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Bentley, A.M., Normand, G., Hoyt, J. and King, R.W. (2007) Distinct Sequence Elements of Cyclin B1 Promote Localization to Chromatin, Centrosomes, and Kinetochores during Mitosis. Mol. Biol. Cell, Dec 2007; 18: 4847 - 4858. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Cassimeris, L. and Morabito, J. (2004) TOGp, the Human Homolog of XMAP215/Dis1, Is Required for Centrosome Integrity, Spindle Pole Organization, and Bipolar Spindle Assembly. Mol. Biol. Cell 15 (4): 1580-1590. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Chung, J-S., Sato, K., Dougherty, I.I., Cruz, Jr., P.D. and Ariizumi, K. (2007) DC-HIL is a negative regulator of T lymphocyte activation. Blood. 109(10): p. 4320-4327. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Kheifets, V., Bright, R., Inagaki, K., Schechtman, D. and Mochly-Rosen, D. (2006) Protein Kinase C δ}PKC)-Annexin V Interaction: a required step in δ PKC translocation and function. J. Biol. Chem. 281(32): p. 23218-23226. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Layzer, J.M., McCaffrey, A.P., Tanner, A.K., Huang, Zan, Kay, Mark A. and Sullenger, B.A. (2004) In vivo activity of nuclease-resistant siRNAs. RNA 10 (5): p. 766-771. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Pomerening, J.R., Ubersax, J.A. and Ferrell, J.E., Jr. (2008) Rapid Cycling and Precocious Termination of G1 Phase in Cells Expressing CDK1AF. Mol. Biol. Cell, 19: 3426 - 3441. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Shulga, N. and Pastorino, J.G. (2006) Acyl Coenzyme A-binding Protein Augments Bid-induced Mitochondrial Damage and Cell Death by Activating μ-Calpain. J. Biol. Chem. 281(41): 30824-30833. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Witherow, D.S., Garrison, T.R., Miller, W.E. and Robert J. Lefkowitz (2004) {beta}-Arrestin inhibits NF-{kappa}B activity by means of its interaction with the NF-{kappa}B inhibitor I{kappa}B{alpha}. Proc. Natl. Acad. Sci. USA 101: 8603-8607. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Yang, Q-H, Church-Hajduk, R., Ren, J., Newton, M.L. and Du, C. (2003) Omi/HtrA2 catalytic cleavage of inhibitor of apoptosis (IAP) irreversibly inactivates IAPs and facilitates caspase activity in apoptosis. Genes & Dev. 17: 1487 - 1496. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Zhu, S., Wang, W., Clarke, D.C. and Liu, X. (2007) Activation of Mps1 Promotes Transforming Growth Factor-β-independent Smad Signaling. J. Biol Chem. 282 (25) 18327-18338. |
| HepG2 | Hepatocellular Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Pastorino, J.G. and Shulga, N. (2008) TNFalpha can provoke cleavage and activation of sterol regulatory element binding protein in ethanol exposed cells via a caspase dependent pathway that is cholesterol-insensitive. J. Biol. Chem. 283: 25638 - 25649. |
| HL-60 | Peripheral Blood Promyelocytic Leukemia | Human | GeneSilencer siRNA Transfection Reagent | Smirnova, I.V., Kajstura, M., Sawamura, T. and Goligorsky, M.S. (2004) Asymmetric dimethylarginine upregulates LOX-1 in activated macrophages: role in foam cell formation. AJP: Heart 287(2): p. H782-H790 |
| HMEC-1 | Human Microvascular Endothelial Cells | Human | GeneSilencer siRNA Transfection Reagent | Zhou, X., Stuart, A., Dettin, L.E., Rodriguez, G., Hoel, B. and Gallicano G.I. (2004) Desmoplakin is required for microvascular tube formation in culture. J. Cell Sci. 117: 3129-3140. |
| HPAEC | Pulmonary Artery Endothelium | Human | GeneSilencer siRNA Transfection Reagent | Berdyshev, E.V., Gorshkova, I., Skobeleva, A., Bittman, R., Lu, X., Dudek, S.M., Mirzapoiazova, T., Garcia, J.G.N. and Natarajan, V. (2009) FTY720 inhibits ceramide synthases and upregulates dihydrosphingosine-1-phosphate formation in human lung endothelial cells. J. Biol. Chem. 284 (9): 5467-5477. |
| HT29 | Colon Adenocarcinoma | Human | GeneSilencer siRNA Transfection Reagent | Yao, K., Shida, S., Selvakumaran, M., Zimmerman, R., Simon, E., Schick, J., Haas, N.B. Balke, M., Ross, H., Johnson, S.W. and O"Dwyer, P.J. (2005) Macrophage Migration Inhibitory Factor Is a Determinant of Hypoxia-Induced Apoptosis in Colon Cancer Cell Lines. Clin. Can. Res. 11: 7264-7272. |
| HUT-78 | T Cells | Human | GeneSilencer siRNA Transfection Reagent | Maneechotesuwan, K., Xin, Y., Ito, K., Jazrawi, E., Lee, K-Y., Usmani, O.S., Barnes, P.J. and Adcock, I.M. (2007) Regulation of Th2 Cytokine Genes by p38 MAPK-Mediated Phosphorylation of GATA-3. J. Immunol. 178(4): 2491-2498. |
| JAR | Choriocarcinoma | Human | GeneSilencer siRNA Transfection Reagent | Abboud-Jarrous, G., Atzmon, R., Peretz, T., Palermo, C., Gadea, B.B., Joyce, J.A. and Vlodavsky, I. (2008) Cathepsin L Is Responsible for Processing and Activation of Proheparanase through Multiple Cleavages of a Linker Segment. J. Biol. Chem. 283: 18167 - 18176. |
| Jurkat | T-Cell Lymphoma | Human | GeneSilencer siRNA Transfection Reagent | Nguyen, J.T. and Wells, J.A. (2003) Direct activation of the apoptosis machinery as a mechanism to target cancer cells. Proc. Natl. Acad. Sci. 100: 7533 7538. |
| KATOIII | Gastric Epithelium | Human | GeneSilencer siRNA Transfection Reagent | Nagasako, T., Sugiyama, T., Mizushima, T., Miura, Y., Kato, M. and Asaka, M. (2003) Up-regulated Smad5 Mediates Apoptosis of Gastric Epithelial Cells Induced by Helicobacter pylori Infection. J. Biol. Chem: 278: 4821 - 4825. |
| L/Stab-2 | Fibroblast | Mouse | GeneSilencer siRNA Transfection Reagent | Park, S-Y., Kang, K-B., Thapa, N., Kim, S-Y., Lee, S-J. and Kim, I-S. (2008) Requirement of Adaptor Protein GULP during Stabilin-2-mediated Cell Corpse Engulfment. J. Biol. Chem. 283(16): 10593-10600. |
| LNCaP | Prostate Cancer | Human | GeneSilencer siRNA Transfection Reagent | Takayama, K., Tsutsumi, S., Suzuki, T., Horie-Inoue, K., Ikeda, K., Kaneshiro, K. Fujimura, T., Kumagai, J., Urano, T., Sakaki, Y., Shirahige, K., Sasano, H., Takahashi, S., Kitamura, T., Ouchi, Y., Aburatani, H. and Inoue, S. (2009) Amyloid precursor protein is a primary androgen target gene that promotes prostate cancer growth. Cancer Res 1 69(1): p. 137. |
| M2 | Melanoma | Human | GeneSilencer siRNA Transfection Reagent | Scott, M.G.H., Pierotti, V., Storez, H., Lindberg, E., Thuret, A., Muntaner, O., Labbe-Jullie, C., Pitcher, J.A. and Marullo, S. (2006) Cooperative Regulation of Extracellular Signal-Regulated Kinase Activation and Cell Shape Change by Filamin A and β-Arrestins. Mol. & Cell Biol, 26: 3432-3445. |
| McA-RH 7777 | Hepatoma | Rat | GeneSilencer siRNA Transfection Reagent | Pastorino, J.G. and Shulga, N. (2008) Tumor Necrosis Factor-{alpha} Can Provoke Cleavage and Activation of Sterol Regulatory Element-binding Protein in Ethanol-exposed Cells via a Caspase-dependent Pathway That Is Cholesterol Insensitive. J. Biol. Chem. 283: 25638 - 25649. |
| MCF-7 | Breast Adenocarcinoma | Human | GeneSilencer siRNA Transfection Reagent | Ikeda, K., Ogawa, S., Tsukui, T., Horie-Inoue, K., Ouchi, Y., Kato, S., Muramatsu, M. and Inoue, S. (2004) Protein Phosphatase 5 Is a Negative Regulator of Estrogen Receptor-mediated Transcription. Mol. Endocrinol. 18(5): 1131-1143. |
| MDA MB-231 | Breast Cancer | Human | GeneSilencer siRNA Transfection Reagent | Ge, L., Shenoy, S.K., Lefkowitz, R.J. and DeFea, K.A. (2004) Constitutive protease-activated-receptor-2 mediated migration of MDA MB-231 breast cancer cells requires both beta-arrestin-1 and 2. J. Biol. Chem. 279(53): p. 55419-55424. |
| MDA MB-468 | Breast Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Ge, L., Shenoy, S.K., Lefkowitz, R.J. and DeFea, K.A. (2004) Constitutive protease-activated-receptor-2 mediated migration of MDA MB-231 breast cancer cells requires both beta-arrestin-1 and 2. J. Biol. Chem.279(53): p. 55419-55424. |
| MDA-MB-435 | Melanoma | Human | GeneSilencer siRNA Transfection Reagent | Abboud-Jarrous, G., Atzmon, R., Peretz, T., Palermo, C., Gadea, B.B., Joyce, J.A.and Vlodavsky, I. (2008) Cathepsin L Is Responsible for Processing and Activation of Proheparanase through Multiple Cleavages of a Linker Segment. J. Biol. Chem. 283: 18167 - 18176. |
| MDA-MB-468 | Breast Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Zoudilova, M., Kumar, P., Ge, L., Wang, P., Bokoch, G. M. and DeFea, K. A. (2007) β-arrestin-dependent regulation of the cofilin pathway downstream of protease-activated receptor-2. J. Biol. Chem. 282: 20634 - 20646. |
| MG63 | Osteosarcoma | Human | GeneSilencer siRNA Transfection Reagent | Ichikawa, T., Horie-Inoue, K., Ikeda, K., Blumberg, B. and Inoue, S. (2007) Vitamin K2 induces phosphorylation of protein kinase A and expression of novel target genes in osteoblastic cells. J Mol Endocrinol 1 39 (4): 239. |
| MKN 28 | Gastric Epithelium | Human | GeneSilencer siRNA Transfection Reagent | Nagasako, T., Sugiyama, T., Mizushima, T., Miura, Y., Kato, M. and Asaka, M. (2003) Up-regulated Smad5 Mediates Apoptosis of Gastric Epithelial Cells Induced by Helicobacter pylori Infection. J. Biol. Chem.: 278: 4821 - 4825. |
| MKN 45 | Gastric Epithelium | Human | GeneSilencer siRNA Transfection Reagent | Nagasako, T., Sugiyama, T., Mizushima, T., Miura, Y., Kato, M. and Asaka, M. (2003) Up-regulated Smad5 Mediates Apoptosis of Gastric Epithelial Cells Induced by Helicobacter pylori Infection. J. Biol. Chem.: 278: 4821 - 4825. |
| MV-4-11 | Biophenotypic B Myelomonocytic Leukemia | Human | GeneSilencer siRNA Transfection Reagent | Yang, X., Liu, L., Sternberg, D., Tang, L., Galinsky, I., DeAngelo, D. and Stone, R. (2005) The FLT3 Internal Tandem Duplication Mutation Prevents Apoptosis in Interleukin-3-Deprived BaF3 Cells Due to Protein Kinase A and Ribosomal S6 Kinase 1-Mediated BAD Phosphorylation at Serine 112. Cancer Res. 65(16): p. 7338-7347. |
| Neuro2a | Neuroblastoma | Mouse | GeneSilencer siRNA Transfection Reagent | Bertling, E., Hotulainen, P., Mattila, P.K., Matilainen, T., Salminen, M., and Lappalainen, P. (2004) Cyclase-associated-protein 1 (CAP1) promotes cofilin-induced actin dynamics in mammalian nonmuscle cells. Mol. Biol. Cell 15 (5): p. 2324-2334. |
| NIH-3T3 | Fibroblast | Mouse | GeneSilencer siRNA Transfection Reagent | Bertling, E., Hotulainen, P., Mattila, P.K., Matilainen, T., Salminen, M., and Lappalainen, P. (2004) Cyclase-associated-protein 1 (CAP1) promotes cofilin-induced actin dynamics in mammalian nonmuscle cells. Mol. Biol. Cell 15 (5): 2324-2334. |
| NIH-3T3 | Fibroblast | Mouse | GeneSilencer siRNA Transfection Reagent | Hotulainen, P., Paunola, E., Vartiainen, M.K. and Lappalainen, P. (2005) Actin-depolymerizing Factor and Cofilin-1 Play Overlapping Roles in Promoting Rapid F-Actin Depolymerization in Mammalian Nonmuscle Cells. Mol. Biol. Cell. 16(2): p. 649-664. |
| NIH-3T3 | Fibroblast | Mouse | GeneSilencer siRNA Transfection Reagent | Nomachi, A., Nishita, M., Inaba, D., Enomoto, M., Hamasaki, M. and Minami, Y. (2008) Receptor Tyrosine Kinase Ror2 Mediates Wnt5a-induced Polarized Cell Migration by Activating c-Jun N-terminal Kinase via Actin-binding Protein Filamin A. J. Biol. Chem. 283(41): 27973-27981. |
| OK | Kidney | Opossum | GeneSilencer siRNA Transfection Reagent | Khundmiri, S.J., Ameen, M., Delamere, N.A. and Lederer, E.D. (2008) PTH-mediated regulation of Na+ -K+ -ATPase requires Src kinase-dependent ERK Phosphorylation. Am J Physiol Renal Physiol. 295(2): F426-F437. |
| OK | Kidney | Opossum | GeneSilencer siRNA Transfection Reagent | Khundmiri, S.J., Dean, W.L., McLeish, K.R. and Lederer, E.D. (2005) Parathyroid Hormone-mediated Regulation of Na+-K+-ATPase Requires ERK-dependent Translocation of Protein Kinase C{alpha}. J. Biol. Chem. 280(10): p. 8705-8713. |
| PC-12 | Pheochromocytoma | Rat | GeneSilencer siRNA Transfection Reagent | de Barry, J., Janoshazi, A., Luc Dupont, J., Procksch, O., Chasserot-Golaz, S., Jeromin, A. and Vitale, N. (2006) Functional Implication of Neuronal Calcium Sensor-1 and Phosphoinositol 4-Kinase-β Interaction in Regulated Exocytosis of PC12 Cells. J. Biol Chem. 281(26): 18098-18111. |
| Primary | Cortical Neurons | Mouse | GeneSilencer siRNA Transfection Reagent | Aarts, M., Lihara, K., Wei, W-L, Xiong, Z-G, Arundine, M., Cerwinski, W., MacDonald, J.F., Tymianski, M. (2003) A Key Role for TRPM7 Channels in Anoxic Neuronal Death. Cell 115: 863. |
| Primary | Cortical Neurons | Mouse | GeneSilencer siRNA Transfection Reagent | Aleyasin, H., Cregan, S.P., Iyirhiaro, G., OHare, M.J., Callaghan, S.M., Slack, R.S. and Park, D.S. (2004) Nuclear Factor-B Modulates the p53 Response in Neurons Exposed to DNA Damage. J. Neurosci. 24 (12): 2963-2973. |
| Primary | Hippocampal Neurons | Rat | GeneSilencer siRNA Transfection Reagent | Amaral, M.D. and Pozzo-Miller, L. (2007) TRPC3 Channels Are Necessary for Brain-Derived Neurotrophic Factor to Activate a Nonselective Cationic Current and to Induce Dendritic Spine Formation. J. Neurosci. 27(19): p. 5179-5189. |
| Primary | Macrophage | Human | GeneSilencer siRNA Transfection Reagent | Asmis, R., Wang, Y., Xu, L., Kisgati, M., Begley, J.G. and驴Mieyal, J.J. (2005) A novel thiol oxidation-based mechanism for adriamycin-induced cell injury in human macrophages. FASEB J. published 13 September 2005, 10.1096/fj.04-2991fje. |
| Primary | Pulmonary Artery Endothelium | Human | GeneSilencer siRNA Transfection Reagent | Birukova, A.A., Chatchavalvanich, S., Rios, A., Kawkitinarong, K., Garcia, J.G.N. and Birukov, K.G. (2006) Differential Regulation of Pulmonary Endothelial Monolayer Integrity by Varying Degrees of Cyclic Stretch. Am. J. Pathol. 168: 1749 - 1761. |
| Primary | Cortical Neurons | Mouse | GeneSilencer siRNA Transfection Reagent | Burkhalter, J., Fiumelli, H., Erickson, J.D. and Martin, J-L. (2007) A critical role for system a amino acid transport in the regulation of dendritic development by BDNF. J Biol Chem 282: 5152 - 5159. |
| Primary | Dendritic Cells (Bone Marrow) | Mouse | GeneSilencer siRNA Transfection Reagent | Chung, J-S, Sato, K., Dougherty, I.I., Cruz, Jr., P.D. and Ariizumi, K. (2007) DC-HIL is a negative regulator of T lymphocyte activation. Blood. 109(10): p. 4320-4327. |
| Primary | Cortical Neurons | Mouse | GeneSilencer siRNA Transfection Reagent | Cui, H., Hayashi, A., Sun, H-S, Belmares, M.P., Cobey, C., Phan, T., Schweizer, J., Salter, M.W., Wang, Y.T., Tasker, R.A., Garman, D., Rabinowitz, J., Lu, P.S. and Tymianski, M. (2007) PDZ Protein Interactions Underlying NMDA Receptor-Mediated Excitotoxicity and Neuroprotection by PSD-95 Inhibitors. J. Neurosci. 27(37): 9901-9915. |
| Primary | Coronary Artery Endothelial | Human HCAEC | GeneSilencer siRNA Transfection Reagent | Dandapat, A., Hu, C., Sun, L. and Mehta, J.L. (2007) Small Concentrations of oxLDL Induce Capillary Tube Formation From Endothelial Cells via LOX-1 Dependent Redox-Sensitive Pathway. Arterioscler Thromb Vasc Biol. |
| Primary | Peritoneal Macrophage | Mouse | GeneSilencer siRNA Transfection Reagent | de Beer, M.C., Zhao, Z., Webb, N.R., van der Westhuyzen, D.R. and de Villiers, W.J.S. (2003) Lack of a direct role for macrosialin in oxidized LDL metabolism J. Lipid Res. 44: 674 - 685. |
| Primary | Dorsal Root Ganglion Neurons | Mouse | GeneSilencer siRNA Transfection Reagent | Haruhisa Higuchi, Toshihide Yamashita, Hideki Yoshikawa, and Masaya Tohyama (2003) Functional inhibition of the p75 receptor using a small interfering RNA. Biochem. & Biophys. Res. Comm. 301: 804809. |
| Primary | Retinal Ganglion | Rat | GeneSilencer siRNA Transfection Reagent | Hayashi, H., Campenot, R.B., Vance, D.E. and Vance, J.E. (2007) Apolipoprotein E-Containing Lipoproteins Protect Neurons from Apoptosis via a Signaling Pathway Involving Low-Density Lipoprotein Receptor-Related Protein-1. J. Neurosci. 27(8): 1933-1941. |
| Primary | Dorsal Root Ganglion | Rat | GeneSilencer siRNA Transfection Reagent | Hengst, U. Cox, L.J., Macosko, E.Z. and Jaffrey, S.R. (2006) Functional and Selective RNA Interference in Developing Axons and Growth Cones. J. Neurosci. 26(21): 5727-5732. |
| Primary | Schwann Cells | Mouse | GeneSilencer siRNA Transfection Reagent | Higuchi, H., Yamashita, T., Yoshikawa, H. and Tohyama, M. (2003) Functional inhibition of the p75 receptor using a small interfering RNA. Biochem. & Biophys. Res. Comm. 301: 804809. |
| Primary | Cardiac Fibroblast | Mouse | GeneSilencer siRNA Transfection Reagent | Hu, C., Dandapat, A., Sun, L., Khan, J.A., Liu, Y., Hermonat, P.L. and Mehta, J.L. (2008) Regulation of TGFbeta 1-mediated collagen formation by LOX-1: Studies based on forced over-expression of TGFbeta 1 in wild-type and LOX-1 knockout mice cardiac fibroblasts. J. Biol. Chem. 283 (16) 10226-10231. |
| Primary | Cardiac Myocytes (Neonatal) | Rat | GeneSilencer siRNA Transfection Reagent | Juhaszova, M., Zorov, D.B., Kim, S-H, Pepe, S., Fu, O., Fishbein, K.W., Ziman, B.D., Wang, S., Ytrehus, K., Antos, C.L., Olson, E.N. and Sollott, S.J. (2004) Glycogen synthase kinase-3{beta} mediates convergence of protection signaling to inhibit the mitochondrial permeability transition pore. J. Clin. Invest. 113(11): p. 1535-1549. |
| Primary | Lung Microvascular Endothelial Cells | Human | GeneSilencer siRNA Transfection Reagent | Kolosova, I.A., Ma, S-F, Adyshev, D.M., Wang, P., Ohba, M., Natarajan, V., Garcia, J.G.N. and Verin, A.D. (2004) Role of CPI-17 in the regulation of endothelial cytoskeleton AJP: Lung. 287(5): L970. |
| Primary | Aortic Muscle Cells | Rat | GeneSilencer siRNA Transfection Reagent | Lake, A.C. & Castellot, J.J. (2003) CCN5 modulates the antiproliferative effect of heparin and regulates cell motility in vascular smooth muscle cells. Cell Comm. & Signaling 1: 5. |
| Primary | Dendritic Cells | Mouse | GeneSilencer siRNA Transfection Reagent | Li, M., Qian, H., Ichim, T.E., Ge, W-W, Popov, I.A., Rycerz, K., Neu, J., White, D., Zhong, R., Min, W-P. (2004) Induction of RNA Interference in Dendritic Cells. Immunologic Research 30 (2) 215-230. |
| Primary | Dendritic Cells | Mouse | GeneSilencer siRNA Transfection Reagent | Li, M., Zhang, X., Zheng, X., Lian, D., Zhang, Z-X., Ge, W., Yang, J., Vladau, C., Suzuki, M., Chen, D., Zhong, R., Garcia, B., Jevnikar, A.M. and Min, W-P (2007) Immune Modulation and Tolerance Induction by RelB-Silenced Dendritic Cells through RNA Interference. J. Immunol. 178(9): p. 5480-5487. |
| Primary | Dendritic Cells | Mouse | GeneSilencer siRNA Transfection Reagent | Liu, G., Ng, H., Akasaki, Y., Yuan, X., Ehtesham, M., Yin, D., Black , K.L. and Yu, J.S. (2004) Small interference RNA modulation of IL-10 in human monocyte-derived dendritic cells enhances the Th1 response. Eur. J. Immunol. 34: 1680-1687. |
| Primary | Cerebellar Neurons | Rat | GeneSilencer siRNA Transfection Reagent | Numakawa, T., Nakayama, H., Suzuki, S., Kubo, T., Nara, F., Numakawa, Y., Yokomaku, D., Araki, T., Ishimoto, T., Ogura, A. and Taguchi, T. (2003) Nerve growth factor-induced glutamate release is via p75 receptor, ceramide and Ca2+ from ryanodine receptor in developing cerebellar neurons. J. Biol. Chem: 278: 41259-41269. |
| Primary | Osteoblast | Mouse | GeneSilencer siRNA Transfection Reagent | Ohyama, Y., Nifuji, A., Maeda, Y., Amagasa, T. and Noda, M. (2004) Spaciotemporal Association and Bone Morphogenetic Protein Regulation of Sclerostin and Osterix Expression during Embryonic Osteogenesis. Endocrinology. 145(10): p. 4685-4692. |
| Primary | HMVEC | Human | GeneSilencer siRNA Transfection Reagent | Radu Stefanescu, Dustin Bassett, Rozbeh Modarresi, Francisco Santiago, Mohamad Fakruddin, and Jeffrey Laurence (2008) Synergistic interactions between interferon-{gamma} and TRAIL modulate c-FLIP in endothelial cells, mediating their lineage-specific sensitivity to thrombotic thrombocytopenic purpura plasma-associated apoptosis. Blood 112: 340 - 349. |
| Primary | Cardiac Myocytes | Feline | GeneSilencer siRNA Transfection Reagent | Ramabadran, R. S., Chancey, A., Vallejo, J.G., Barger, P.M., Sivasubramanian, N. and Mann, D.L. (2008) Targeted Gene Silencing of Tumor Necrosis Factor Attenuates the Negative Inotropic Effects of Lipopolysaccharide in Isolated Contracting Cardiac Myocytes. Tex Heart Inst J. 35(1): 16-21. |
| Primary | T-Cells (CD3+) | Human | GeneSilencer siRNA Transfection Reagent | Samten, B., Howard, S.T., Weis, S.E., Wu, S., Shams, H., Townsend, J.C., Safi, H. and Barnes, P.F. (2005) Cyclic AMP Response Element-Binding Protein Positively Regulates Production of IFN-{gamma} by T Cells in Response to a Microbial Pathogen. J. Immunol. 174(10): p. 6357-6363. |
| Primary | Cortical Neurons | Mouse | GeneSilencer siRNA Transfection Reagent | Sato, S., Xu, J., Okuyama, S., Martinez, LB., Walsh, S.M, Jacobsen, M.T, Swan, R.J., Schlautman, J.D., Ciborowski, P. and Ikezu, T. (2008) Spatial learning impairment, enhanced CDK5/p35 activity, and downregulation of NMDA receptor expression in transgenic mice expressing tau-tubulin kinase 1. J Neurosci 28(53): 14511. |
| Primary | Dendritic Cells | Human | GeneSilencer siRNA Transfection Reagent | Smith, A.L., Ganesh, L., Leung, K., Jongstra-Bilen, J., Jongstra, J. and Nabel, G.J. (2007) Leukocyte-specific protein 1 interacts with DC-SIGN and mediates transport of HIV to the proteasome in dendritic cells. J. Exp. Med. 204(2): p. 421-430. |
| Primary | Cortical Neurons | Mouse | GeneSilencer siRNA Transfection Reagent | Supnet, C., Grant, J., Kong, H., Westaway, D. and Mayne, M. (2006) Amyloid β-(1-42) Increases Ryanodine Receptor-3 Expression and Function in TgCRND8 Mice. J. Biol. Chem. 281: 38440 - 38447. |
| Primary | Aveolar Type II Cells | Mouse | GeneSilencer siRNA Transfection Reagent | Ueno, T., Linder, S., Na, C-L, Rice, W.R., Johansson, J., and Weaver, T.E. (2004) Processing of Pulmonary Surfactant Protein B by Napsin and Cathepsin H. J. Biol. Chem. 279: 16178-16184. |
| Primary | Macrophage | Mouse | GeneSilencer siRNA Transfection Reagent | Wang, Y., Chen, T., Han, C., He, D., Liu, H., An, H., Cai, Z. and Cao, X. (2007) Lysosome-associated small Rab GTPase Rab7b negatively regulates TLR4 signaling in macrophages by promoting lysosomal degradation of TLR4. Blood 110: 962 - 971. |
| Primary | Dorsal Root Ganglion | Rat | GeneSilencer siRNA Transfection Reagent | Wu, K.Y., Hengst, U., Cox, L.J., Macosko, E.Z., Jeromin, A., Urquhart, E.R., and Jaffrey, S.R. (2005) Local translation of RhoA regulates growth cone collapse. Nature 436: 1020-1024. |
| Primary | Lacrimal Gland Acinar Cells | Rabbit | GeneSilencer siRNA Transfection Reagent | Xie, J., Chiang, L., Contreras, J., Wu, K., Garner, J.A., Medina-Kauwe, L. and Hamm-Alvarez, S.F. (2006) Novel Fiber-Dependent Entry Mechanism for Adenovirus Serotype 5 in Lacrimal Acini. J. Virol. 80(23): 11833 - 11851. |
| Primary | Bone Marrow | Mouse | GeneSilencer siRNA Transfection Reagent | Yang, R., Cai, Z., Zhang, Y., Yutzy IV, W.H., Roby, K.F. and Roden, R.B.S. (2006) CD80 in Immune Suppression by Mouse Ovarian Carcinoma驴Associated Gr-1+CD11b+ Myeloid Cells. Cancer Res 66 (13): 6807 - 6815. |
| Primary | Dendritic Cells | Mouse | GeneSilencer siRNA Transfection Reagent | Zheng, X., Koropatnick, J., Li, M., Zhang, X., Ling, F., Ren, X., Hao, X., Sun, H., Vladau, C., Franek, J.A., Feng, B., Urquhart, B.L., Zhong, R., Freeman, D.J., Garcia, B. and Min, W-P. (2006) Reinstalling Antitumor Immunity by Inhibiting Tumor-Derived Immunosuppressive Molecule IDO through RNA Interference. J. Immunol. 177(8): 5639-5646. |
| Primary | Pulmonary Artery Endothelium | Human | GeneSilencer siRNA Transfection Reagent | Zhuowei Li, Xhevahire Hyseni, Jacqueline D. Carter, Joleen M. Soukup, Lisa A. Dailey, and Yuh-Chin T. Huang (2006) Pollutant particles enhanced H2O2 production from NAD(P)H oxidase and mitochondria in human pulmonary artery endothelial cells. Am J Physiol Cell Physiol 291: C357 - C365. |
| Primary HPAEC | Pulmonary Artery Endothelium | Human | GeneSilencer siRNA Transfection Reagent | Rentsendorj, O., Mirzapoiazova, T., Adyshev, D., Servinsky, L.E., Renne, T., Verin, A.D. and Pearse, D.B. (2008) Role of vasodilator-stimulated phosphoprotein in cGMP-mediated protection of human pulmonary artery endothelial barrier function. Am J Physiol Lung Cell Mol Physiol. 294(4): L686 -L697. |
| RAW 264.7 | Macrophage | Mouse | GeneSilencer siRNA Transfection Reagent | de Beer, M.C., Zhao, Z., Webb, N.R., van der Westhuyzen, D.R. and de Villiers, W.J.S. (2003) Lack of a direct role for macrosialin in oxidized LDL metabolism. J. Lipid Res. 44: 674 - 685. |
| RAW 264.7 | Macrophage | Mouse | GeneSilencer siRNA Transfection Reagent | Liu, X., Yao, M., Li, N., Wang, C., Zheng, Y. and Cao, X. (2008) CaMKII promotes TLR-triggered proinflammatory cytokine and type I interferon production by directly binding and activating TAK1 and IRF3 in macrophages. Blood. 112(13): 4961-4970. |
| Renca | Renal Cell Carcinoma | Mouse | GeneSilencer siRNA Transfection Reagent | Ogushi, T., Takahashi, S., Takeuchi, T., Urano, T., Horie-Inoue, K., Kumagai, J., Kitamura, T., Ouchi, Y., Muramatsu, M. and Inoue, S. (2005) Estrogen Receptor-Binding Fragment-Associated Antigen 9 Is a Tumor-Promoting and Prognostic Factor for Renal Cell Carcinoma. Cancer Res. 65(9): p. 3700-3706. |
| SaOs2 | Osteosarcoma | Human | GeneSilencer siRNA Transfection Reagent | Chu, F.,Chou, P.M., Zheng, X., Mirkin, B.L. and Rebbaa, A.驴(2005)驴Control of Multidrug Resistance Gene mdr1 and Cancer Resistance to Chemotherapy by the Longevity Gene sirt1. Cancer Res.65 (22): p. 10183-10187. |
| SaOS2 | Osteosarcoma | Human | GeneSilencer siRNA Transfection Reagent | Zheng, X., Chu, F., Chou, P.M., Gallati, C., Dier, U., Mirkin, B.L., Mousa, S.A. and Rebbaa, A. (2009) Cathepsin L inhibition suppresses drug resistance in vitro and in vivo: a putative mechanism. Am J Physiol Cell Physiol. 296(1): C65-C74. |
| Saos-2 | Osteosarcoma | Human | GeneSilencer siRNA Transfection Reagent | Zheng, X., Chou, P.M., Mirkin, B.L. and Rebbaa, A. (2004) Senescence-initiated Reversal of Drug Resistance: Specific Role of Cathepsin L. Cancer Res. 64: 1773 - 1780. |
| SCCH196 | Sarcoma | Human | GeneSilencer siRNA Transfection Reagent | Takahashi, A., Higashino, F., Aoyagi, M., Yoshida, K., Itoh, M., Kyo, S., Ohno, T., Taira, T., Ariga, H., Nakajima, K., Hatta, M., Kobayashi, M., Sano, H., Kohgo, T. and Shindoh, M., (2003) EWS/ETS Fusions Activate Telomerase in Ewings Tumors. Cancer Res. 63: 8338-8344. |
| SNB19 | Glioblastoma | Human | GeneSilencer siRNA Transfection Reagent | Song, S.W., Fuller, G.N., Zheng, H. and Zhang, W. (2005) Inactivation of the Invasion Inhibitory Gene IIp45 by Alternative Splicing in Gliomas. Cancer Res. 65(9): p. 3562-3567. |
| SW480 | Colon Adenocarcinoma | Human | GeneSilencer siRNA Transfection Reagent | Zhu, S., Wang, W., Clarke, D.C. and Liu, X. (2007) Activation of Mps1 Promotes Transforming Growth Factor-β-independent Smad Signaling. J. Biol. Chem. 282(25): 18327-18338. |
| U20S | Osteosarcoma | Human | GeneSilencer siRNA Transfection Reagent | Hotulainen P. and Lappalainen, P. (2006) Stress fibers are generated by two distinct actin assembly mechanisms in motile cells J. Cell Biol. 173(3): 383-394. |
蚂蚁淘电商平台
ebiomall.com
ebiomall.com
公司简介
蚂蚁淘（www.ebiomall.cn）是中国大陆目前唯一的生物医疗科研用品B2B跨境交易平台，
该平台由多位经验丰富的生物人和IT人负责运营。蚂蚁淘B2B模式是指客户有采购意向后在蚂蚁
淘搜索全球供应信息，找到合适的产品后在蚂蚁淘下单，然后蚂蚁淘的海外买手进行跨境采购、
运输到中国口岸，最后由蚂蚁淘国内团队报关运输给客户...
蚂蚁淘承诺
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蚂蚁淘所有产品都是自运营的，我们已经跟国外多家厂方建立品牌推广合作关系, 获得对方的支持和授权； 同时客户可以通过订单详情查看到货物从厂方至客户的所有流程， 确保货物的来源； 正规报关，提供13%增值税发票。
及时交付： 限时必达 畅选无忧
蚂蚁淘的运营团队都是有着多年经验的成员，他们熟悉海外采购、仓储物流、报关等环节； 同时通过在线的流程监控，蚂蚁淘的进口速度比传统企业提高了50%以上， 部分产品甚至能做到7-10天到货，即蚂蚁淘的“时必达”服务。
轻松采购： 在线下单 简单省事
蚂蚁淘的价格是真实透明的，并且具有很大的价格优势，不需要繁杂的询价比价； 报价单与合同可以直接在线生成或打印；就像在京东购物一样， 您的鼠标点击几 次即完成在蚂蚁淘的采购，订单详情会告诉您所有进程。
售后申请： 耐心讲解 优质服务
蚂蚁淘提供的产品在使用过程中如因产品质量问题有售后需求时， 您可通过我的订单提交您的“申请售后”， 蚂蚁淘产品顾问会第一时间为您处理， 在售后服务过程中如遇到问题也可致电蚂蚁淘客服热线：4000-520-616。
2021-07-21
间隙时间设定：按设置键，显示窗-1，说明进入间歇时间设定状态，此时显示窗口所显示的是原始数据或“——”(以下同)，此次输入数据单位为秒。 工作时间设定：间歇时间设定完成后，按设置键，显示窗-2，说明进入工作时间设定状态，此时显示窗口所显示的是原始数据， 查看更多
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2018-03-06
北京海诚中仪科技有限公司在发布的宁波新芝超声波细胞粉碎机JY98-IIIN供应信息，浏览与宁波新芝超声波细胞粉碎机JY98-IIIN相关的产品或在搜索更多与宁波新芝超声波细胞粉碎机JY98-IIIN相关的内容。 查看更多
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2021-08-02
超声波细胞破碎仪就是将电能通过换能器转换为声能，这种能量通过液体介质而变成一 查看更多
>
2017-08-04
超声波细胞粉碎机是利用超声波在液体中的分散效应，使液体产生空化的作用，从而使液体中的固体颗粒或细胞组织破碎．... 查看更多
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2021-07-23
打开电源开关(机箱后面板左下角)，此时屏幕显示“新芝商标”。按( )键，到换能器选择界面,即图4所示(如果不按( )键，屏幕将停留在启动界面) 从启动界面进入换能器选择界面，首先必须选择变幅杆的型号，即图4中“变幅杆：ΦX”中的X会不停的跳动，请选择与您安装上去 查看更多
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2021-09-18
[db:简介] 查看更多
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2021-09-07
最大超声功率输出：250W
主机尺寸：270*200*140mm
控制模式：自动、手动可切换(可选配脚踏开关
温度控制：超声保护报警功能
脉冲器：闭循环0.1-99s可调 开循环0.1-99S可调 查看更多
>
2021-07-24
一、超声波细胞粉碎机产品面板说明 1、开机并进入初始界面(电源开关在机箱后面右下角); 2、进入变幅杆选择界面进行设置; 3、选择完成后，按下设置键锁定变幅杆; 4、再按下设置键，根据实验需要设置其他参数; 5、参数设置完成后，再按设置键锁定参数; 查看更多
>
2021-09-11
[db:简介] 查看更多
>
2021-09-03
超声波细胞粉碎机是一种利用强超声在液体中产生空化效应，对物质进行超声处理的多功能、多用途的仪器，能用于动植物组织、细胞、细菌、芽胞菌种的破碎，同时可用来乳化、分离、分散、匀化、提取、脱气、清洗及加速化学反应等等。该机广泛应用于生物化学、微生物学、药物化学、表面化学、物理学、动物学、农学、医学、制药等领域教学、科研、生产。 ... 查看更多
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2021-08-29
频率：20-25KHz
功率：950W
破碎容量：0.5-600ml
随机变幅杆：Φ6 查看更多
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常见问题
蚂蚁淘所售产品均为正品吗？
蚂蚁淘的创始人兼CEO是钟定松先生，具有十年的从业经验，在业界享有良好的口碑；
Ebiomall是跨境直采平台，我们直接从厂家采购，自己的团队负责国际物流和清关，中间没有第三方，蚂蚁淘承诺所售产品仅为正品，假一罚十。
下单后可以修改订单吗？
未确认状态的订单可以修改，打开“订单详情”页面，点击右上角的“修改订单”即可，若已审核确定，则订单无法修改。
商品几天可以发货？
现货产品付款审核后即可发货,大部分期货产品在3周左右即可到货,提供时必达服务的产品订单审核十天内即可发货。
订单如何取消？
如订单处于未确定状态，进入“我的订单"页面，找到要取消的订单，点击“取消订单”按钮。
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或拨打4000-520-616，除此之外客户可在 联系我们页面找到更多的联系方式。
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退换货/维修需要多长时间？
一般情况下，退货处理周期为客户收到产品一个月内（以快递公司显示签收时间为准），包装规格、数量、品种不符，外观毁损、短缺或缺陷，请在收到货24小时内申请退换货；特殊商品以合同条款为准。
商品咨询
没有超声破碎仪怎么做原核表达 蛋白质和糖学技术讨论版论坛123
我恨月亮2021-07-27
我们实验室比较简陋，没有超声破碎仪，能做原核表达吗？用溶菌酶代替可以吗
超声波细胞粉碎机使用说明以及注意事项 分析行业新闻123
泽田27丶TA业2017-11-01
专业人士来回答：每一家的产品质量不一样，所以对产品的使用要求也不同。但需要注意的共同事项均为以下几点，请记牢。防潮，不要进水，以免短路；防尘，以免导致静电打火；防摔，防磕碰，以免机械性损坏内部零件；防止空载开机，工具头空载时振幅较大，容易把工具头震裂；防止工具头在使用在碰壁，若是玻璃杯，则容易被超声波击碎，若是金属反应釜，容易把工具头撞坏。所以应该确保工具头处于容器中央；使用中调整工具头顶端与烧杯或其他种类反应釜底部保持3cm以上，并根据物料的处理量来适当调整，保持与容器底部30mm-50mm的间距，可确保物料在容器中高速上下回流，以使得物料被充分搅拌、充分破碎、分散、乳化、反应等；处理完毕物料后，开机用清水冲一下工具头，洗净污物，以便下次使用；
其他诸如设置工作时间，定时，脉冲，连续等，随意，无要求；使用完毕放回干燥清洁的保管箱或工作台；
使用任何产品都要保持良好的使用习惯，注意保养，可提高使用寿命，就跟电视、电脑一样的。
其他诸如设置工作时间，定时，脉冲，连续等，随意，无要求；使用完毕放回干燥清洁的保管箱或工作台；
使用任何产品都要保持良好的使用习惯，注意保养，可提高使用寿命，就跟电视、电脑一样的。
【求助】请问沉淀重悬浮过程中（提取膜蛋白）能用超声破碎仪混匀么...123
alwaysharold12021-08-05
关于细菌超声破碎的一些经验总结123
陡变吧GEK2021-07-23
一是看破碎后菌液的浑浊程度，较清澈的是破碎比较完全的
二是镜检染色观察计数，破碎完全的基本看不到完整的菌体，都是细胞碎片
三是测菌液的电导率，根据电导率大小来判断。
二是镜检染色观察计数，破碎完全的基本看不到完整的菌体，都是细胞碎片
三是测菌液的电导率，根据电导率大小来判断。
教你如何选购合适的超声波细胞破碎仪技术文章上海容威仪器有限公司123
wsw19834572021-08-10
多糖的药理作用研究123
猴汤啃392021-08-13
肯定会的
破碎机哪个牌子好?牌子网123
wangjie9012017-10-31
thermo
超声波破碎仪功率多少才能破碎细胞_问答详情_蚂蚁淘,【正品极速...123
棕油2722017-10-21
探头位置没放好:探头一定要接近底部，约1cm。功率根据仪器不同会有所不同，但你可以观察液面，有波动但不要太剧烈就好。
超声破碎的条件不能一概而论，要看你的实验要求，有的是细菌破碎，有的是对组织细胞进行破碎，要掌握好功率和每次超声时间，功率大时，每次超声时间可缩短，不能让温度升高，必要时在冰浴条件下进行超声破碎。至于每次超声时是否起泡沫到不是关键的问题，这与你超声的量明显有关。
超声破碎的条件不能一概而论，要看你的实验要求，有的是细菌破碎，有的是对组织细胞进行破碎，要掌握好功率和每次超声时间，功率大时，每次超声时间可缩短，不能让温度升高，必要时在冰浴条件下进行超声破碎。至于每次超声时是否起泡沫到不是关键的问题，这与你超声的量明显有关。
【求助/交流】RNAi引物设计问题 分子生物 综合其他 ...123
wengyu11212021-08-11
我们实验室想购一台进口的超声细胞破碎仪，请问哪位仁兄知道哪种比较好，价格如何呢？我找不到相应的讨论版，就在这里发表了，请见谅！！！
急！！！谢谢！！
急！！！谢谢！！
【求助】超声破碎仪能使药物溶解吗? 细胞技术讨论版论坛123
dululu2021-08-14
挥发油+DMSO用超声破碎仪能溶解？？？？？？？？
溶解后加培养液稀释不会出现悬浮浑浊吗？？？？？？
溶解后加培养液稀释不会出现悬浮浑浊吗？？？？？？
上海供应非接触式超声破碎仪丨非接触式全自动超声破碎仪价格 ...123
兔儿爷嗻nk32017-10-12
时间长了容易发热烧坏。
超声波细胞破碎仪使用注意事项:新芝生物123
牛牛n7Q2021-08-06
进行活细胞植发技术前5天，不要使用阿司匹林药物，进行活细胞植发技术前要对身体进行检查，保证身体健康，精神正常，而且手术者要正确看待手术效果。女性在进行活细胞注射前要避开月经期，而且在手术前要洗澡，保持身体的清洁。在进行植发前要保证手术部位吴局部感染病灶。
