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Description
Data
siRNA Design
Citations
- High siRNA transfection efficiency
- Functional gene silencing post siRNA delivery
- Compatibility with diverse growth conditions (with and without serum)
- Low cytotoxicity
- Easy-to-use protocols for both adherent and suspension cells
Contents
- Hydrated GeneSilencer Lipid
- siRNA Diluent (corresponding ratio for each kit)
Storage
GeneSilencer siRNA Transfection Reagent is shipped at room temperature, as similar to other gene therapy products. For maximum stability, store all reagents at 4篊 upon receipt. If stored properly, all components are stable for 6 months.GeneSilencer Transfection Reagent Kit
- 50 rxn kit, 0.18 ml, T500020
- 200 rxn kit, 0.75 ml, T500750
- 1000 rxn kit, 5 x 0.75 ml, T505750
- 10 rxn sample kit, 0.03 ml, T500020S
Long Noncoding RNA pancEts-1 Promotes Neuroblastoma Progression through hnRNPK-Mediated β-Catenin Stabilization.Li, D.,et al (2018) Cancer Res. 78 (5): 1169-1183. Link
Apoptotic cells trigger the ABCA1/STAT6 pathway leading to PPAR‐γ expression and activation in macrophages.Kim, M-J.,et al (2018) J. Leukocyte Biol. 103(5): 885-895. Link
Transient Silencing of DNA Repair Genes Improves Targeted Gene Integration in the Filamentous Fungus Trichoderma reesei.Chum, P.Y.,et al ((2017) Appl. Environ. Microbiol. 83(15): e00535-17. Link
Programming of macrophages by apoptotic cancer cells inhibits cancer progression through exosomal PTEN and PPARγ ligands.Kim, Y-B.,et al (2017) bioRxiv 217562: 1-75. Link
Role of Sphingosine Kinase 1 and S1P Transporter Spns2 in HGF-mediated Lamellipodia Formation in Lung Endothelium.Fu, P.,et al (2016) J. Biol. Chem. 291(53): 27187?27203. Link
Ubiquitin-specific Protease 20 Regulates the Reciprocal Functions of β-Arrestin2 in Toll-like Receptor 4-promoted Nuclear Factor κB (NFκB) Activation.Jean-Charles, P-Y., (2016) J. Biol. Chem. 291 (14): 7450 ?7464. Link
Tolerogenic nanoparticles inhibit T cell杕ediated autoimmunity through SOCS2.Yeste, A.,et al (2016) Sci. Signal. 9(433): RA61. Link
Synthesis of an Endogenous Steroidal Na Pump Inhibitor Marinobufagenin, Implicated in Human Cardiovascular Diseases, Is Initiated by CYP27A1 via Bile Acid Pathway.Fedorova, O.V.,et al (2015) Circ Cardiovasc Genet. 8(5): 736-45. Link
Thioredoxin Activates MKK4-NFκB Pathway in a Redox-dependent Manner to Control Manganese Superoxide Dismutase Gene Expression in Endothelial Cells.Kundumani-Sridharan, V.,et al (2015) J. Biol. Chem. 290(28): 17505?17519. Link
Identification, Mechanism of Action, and Antitumor Activity of a Small Molecule Inhibitor of Hippo, TGF-β, and Wnt Signaling Pathways.Basu, D.,et al (2014) Mol. Cancer Ther. 13(6): 1457-1467. Link
PKC Potentiates Tyrosine Kinase Inhibitors STI571 and Dasatinib Cytotoxic Effect.Tob蚾, A.,et al (2014) Anticancer Res. 34(7): 3347-3356. Link
TREK2 Expressed Selectively in IB4-Binding C-Fiber Nociceptors Hyperpolarizes Their Membrane Potentials and Limits Spontaneous Pain.Acosta, C.,et al (2014) J. Neurosci. 34(4): 1494-1509. Link
RhoA/Phosphatidylinositol 3-Kinase/Protein Kinase B/Mitogen-Activated Protein Kinase Signaling after Growth Arrest朣pecific Protein 6/Mer Receptor Tyrosine Kinase Engagement Promotes Epithelial Cell Growth and Wound Repair via Upregulation of Hepatocyte Growth Factor in Macrophages.Lee, Y-J.,et al (2014) J. Pharma. Exp. Thera. 350(3): 563-577. Link
GeneSilencer Cell Line Citations: 3T3-L1, A549, A7, AGS, ASM Transformed, B16-F1, BEL7404, Bronchial C166, C2C12, C6R, CHO-K1, DC2.4, GS-KB-3-1, H22, H441, H460, H9c2, hCMEC/D3, HCP40, HCT-116, HEK 293, HEK 293T, HEK-293, HeLa, HepG2, HL-60, HMEC-1, HPAEC, HT29, HUT-78, JAR, Jurkat, KATOIII, L/Stab-2, LNCaP, M2, McA-RH 7777, MCF-7, MDA MB-231, MDA MB-468, MDA-MB-435, MG63, MKN 28, MKN 45, MV-4-11, Neuro2a, NIH-3T3, OK, PC-12, Cortical Neurons, Hippocampal Neurons, Macrophage, Pulmonary Artery, Endothelium, Dendritic Cells(Bone Marrow), Cortical Neurons, Coronary Artery Endothelial, Peritoneal Macrophage, Dorsal Root Ganglion Neurons, Retinal Ganglion, Dorsal Root Ganglion, Schwann Cells Cardiac Fibroblast, Cardiac Myocytes (Neonatal), Lung Microvascular Endothelial Cells, Aortic Muscle Cells, Dendritic Cells, Cerebellar Neurons, Osteoblast, HMVEC, Cardiac Myocytes, T-Cells (CD3+), Cortical Neurons, Dendritic Cells, Cortical Neurons, Aveolar Type II Cells, Macrophage, Dorsal Root Ganglion Lacrimal Gland Acinar Cells, Bone Marrow, Dendritic Cells, Pulmonary Artery Endothelium, Primary HPAEC. RAW 264.7, Renca, SaOs2, Saos-2, SCCH196, SNB19, SW480, U20S
GeneSilencerTM Reagent vs. siPORTTM NeoFXTM Reagent
HEK 293 Cells (click on images to enlarge)
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Figure Legend: HEK 293 cells were transfected with 5, 10, 30 or 50 nM of Silencer FAM Labeled GAPDH siRNA with either GeneSilencer (Genlantis) or siPORT NeoFX (Ambion) siRNA transfection reagents according to the manufacturer"s recommended protocols. Cells were incubated for 48 hours then fixed, permeablized, and incubated with AlexaFluor 555 phalloidin (Invitrogen), which stains cellular actin red. Cells were then mounted on slides and stained with DAPI (Invitrogen), which stains the cell nuclei blue. Transfected cells were visualized by fluorescence microscopy using identical exposure times for FITC, TRITC, and DAPI. The transfected GAPDH siRNA is localized in the cell cytosol and can be seen as green fluorescent specks or dots.
HeLa S3 Cells (click on images to enlarge)
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Figure Legend: HeLa S3 cells were transfected with 5, 10, 30 or 50 nM of Silencer FAM labeled GAPDH siRNA with either GeneSilencer (Genlantis) or siPORT&tm; NeoFX&tm; (Ambion) siRNA transfection reagents according to the manufacturer"s recommended protocols. Cells were incubated for 48 hours then fixed, permeablized, and incubated with AlexaFluor 555 phalloidin (Invitrogen), which stains cellular actin red. Cells were then mounted on slides and stained with DAPI (Invitrogen), which stains the cell nuclei blue. Transfected cells were visualized by fluorescence microscopy using identical exposure times for FITC, TRITC, and DAPI. The transfected GAPDH siRNA is localized in the cell cytosol and can be seen as green fluorescent specks or dots.
How to Design siRNA (Small Interfering RNA)
The sequence of the siRNA can be selected as follows:
The sequence selection process has no other constraints. It is important to note that structure within thetargeted mRNA appears to have minimal effect on the availability of the mRNA target and efficacy of thesiRNA silencing approach. To-date, successful silencing has been achieved using the above method toselect the target sequence, although the method is essentially random with respect to accounting for mRNAstructure.Although siRNA silencing appears to be extremely effective by selecting a single target in the mRNA, itmay be desirable to design and employ two independent siRNA duplexes to control for specificity of thesilencing effect. This recommendation is only for specificity for it is yet unknown if the targeting of a geneby two different siRNA duplexes would be more effective than using a single siRNA duplex. It is believedthat the rate-limiting component of the siRNA effect is the availability of cellular nuclease components andnot mRNA target availability. Therefore, doubling the number of siRNA duplexes is not expected to doublethe rate or efficiency of silencing.If the selected siRNA duplex(es) do not function for silencing, the following steps are recommended. First,a search is conducted for sequencing errors in the gene and possible polymorphisms. Initial studies on thespecificity of target recognition by siRNA duplexes indicates that a single point mutation located in thepaired region of an siRNA duplex is sufficient to abolish target mRNA degradation. Second, a reexaminationis performed to confirm whether the cell line is from the expected species. Third, a secondand/or third target are selected and the corresponding siRNA duplexes prepared.
| Cell Line | Cell Type | Source | Genlantis Product | Citation |
| 3T3-L1 | Embryo | Swiss Mouse | GeneSilencer siRNA Transfection Reagent | Mizuarai, S., Miki, S., Araki, H., Takahashi, K. and Kotani, H.(2005) Identification of Dicarboxylate Carrier Slc25a10 as Malate Transporter in de Novo Fatty Acid Synthesis. J. Biol. Chem.280(37): p. 32434-32441. |
| A549 | Lung Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Ito, K., Yamamura, S., Essilfie-Quaye, S., Cosio, B., Ito, M., Barnes, P.J. and Adcock, I.M. (2006) Histone deacetylase 2-mediated deacetylation of the glucocorticoid receptor enables NFκB suppression. J. Exp. Med. 203(1): 7-13. |
| A549 | Lung Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Stepulak, A., Sifringer, M., Rzeski, W., Endesfelder, S., Gratopp, A., Pohl, E.E., Bittigau, P., Hansen, H.H., Stryjecka-Zimmer, M., Turski, L. and Ikonomidou, C. (2005) NMDA antagonist inhibits the extracellular signal-regulated kinase pathway and suppresses cancer growth. Proc. Natl. Acad. Sci. 102: 15605 - 15610. |
| A7 | Melanoma | Human | GeneSilencer siRNA Transfection Reagent | Scott, M.G.H., Pierotti, V., Storez, H., Lindberg, E., Thuret, A., Muntaner, O., Labbe-Jullie, C., Pitcher, J.A. and Marullo, S. (2006) Cooperative Regulation of Extracellular Signal-Regulated Kinase Activation and Cell Shape Change by Filamin A and β-Arrestins. Mol. & Cell Biol, 26: 3432-3445. |
| AGS | Gastric Epithelium | Human | GeneSilencer siRNA Transfection Reagent | Nagasako, T., Sugiyama, T., Mizushima, T., Miura, Y., Kato, M. and Asaka, M. (2003) Up-regulated Smad5 Mediates Apoptosis of Gastric Epithelial Cells Induced by Helicobacter pylori Infection. J. Biol. Chem.: 278: 4821 - 4825. |
| AGS | Gastric Epithelium | Human | GeneSilencer siRNA Transfection Reagent | Varro, A., Noble, P-J. M., Pritchard, D.M., Kennedy, S., Hart, C.A., Dimaline, R. and Dockray, G.J. (2004) Helicobacter pylori Induces Plasminogen Activator Inhibitor 2 in Gastric Epithelial Cells through Nuclear Factor-B and RhoA: Implications for Invasion and Apoptosis Cancer Res. 64: 1695 - 1702. |
| ASM Transformed | Airway Smooth Muscle Cells | Human | GeneSilencer siRNA Transfection Reagent | Tran, T., Ens-Blackie, K., Rector, E.S., Stelmack, G.L., McNeill, K.D., Tarone, G., Gerthoffer, W.T., Unruh, H. and Halayko, A.J. (2007) Laminin-binding Integrin α7 is Required for Contractile Phenotype Expression by Human Airway Myocyte. Am. J. Respir. Cell Mol. Biol. 37(6): 668-80. |
| B16-F1 | Melanoma | Mouse | GeneSilencer siRNA Transfection Reagent | Bertling, E., Hotulainen, P., Mattila, P.K., Matilainen, T., Salminen, M., and Lappalainen, P. (2004) Cyclase-associated-protein 1 (CAP1) promotes cofilin-induced actin dynamics in mammalian nonmuscle cells. Mol. Biol. Cell 15 (5): p. 2324-2334. |
| B16-F1 | Melanoma | Mouse | GeneSilencer siRNA Transfection Reagent | Hotulainen, P., Paunola, E., Vartiainen, M.K. and Lappalainen, P. (2005) Actin-depolymerizing Factor and Cofilin-1 Play Overlapping Roles in Promoting Rapid F-Actin Depolymerization in Mammalian Nonmuscle Cells. Mol. Biol. Cell. 16(2): p. 649-664. |
| BEL7404 | Hepatoma | Human | GeneSilencer siRNA Transfection Reagent | Liang, X-J, Finkel, T., Shen, D-W, Yin, J-J, Aszalos, A. and Gottesman, M.M. (2008) SIRT1 Contributes in Part to Cisplatin Resistance in Cancer Cells by Altering Mitochondrial Metabolism. Mol Cancer Res 6(9): 1499-506. |
| Bronchial | Smooth Muscle | Human | GeneSilencer siRNA Transfection Reagent | Nunes, R.O., Schmidt, M., Dueck, G., Baarsma, H., Halayko, A.J., Kerstjens, H.A.M., Meurs, H. and Gosens, R. (2008) GSK-3/β-catenin signaling axis in airway smooth muscle: role in mitogenic signaling Am J Physiol Lung Cell Mol Physiol. 294: L1110 - L1118. |
| C166 | Yolk Sack Endothelium | Mouse | GeneSilencer siRNA Transfection Reagent | Zhou, X., Stuart, A., Dettin, L.E., Rodriguez, G., Hoel, B. and Gallicano G.I. (2004) Desmoplakin is required for microvascular tube formation in culture. J. Cell Sci. 117: 3129-3140. |
| C2C12 | Myoblast | Mouse | GeneSilencer siRNA Transfection Reagent | Evangelisti, C., Tazzari, P.L., Riccio, M., Fiume, R., Hozumi, Y., Fala, F., Goto, K.., Manzoli, L., Cocco, L. and Martelli, A.M. (2007) Nuclear diacylglycerol kinase-ζ is a negative regulator of cell cycle progression in C2C12 mouse myoblasts. FASEB J. 21: 3297 - 3307 |
| C6R | Glioma | Rat | GeneSilencer siRNA Transfection Reagent | Saarikangas, J., Hakanen, J., Mattila, P.K., Grumet, M., Salminen, M. and Lappalainen, P. (2008) ABBA regulates plasma-membrane and actin dynamics to promote radial glia extension. J Cell Sci. 121(Pt 9):1444-54. |
| CHO-K1 | Ovary | Chinese Hamster | GeneSilencer siRNA Transfection Reagent | Kheifets, V., Bright, R., Inagaki, K., Schechtman, D. and Mochly-Rosen, D. (2006) Protein Kinase C δ PKC-Annexin V Interaction: a required step in δ PKC translocation and function. J. Biol. Chem. 281(32): p. 23218-23226. |
| DC2.4 | Bone Marrow | Mouse | GeneSilencer siRNA Transfection Reagent | Jing, H., Yen, J-H and Ganea, D. (2004) A novel signaling pathway mediates the inhibition of CCL3/4 expression by PGE2. J. Biol. Chem. 279(53): p. 55176-55186. |
| GS-KB-3-1 | Epidermoid Adenocarcinoma | Human | GeneSilencer siRNA Transfection Reagent | Liang, X-J, Finkel, T., Shen, D-W, Yin, J-J, Aszalos, A. and Gottesman, M.M. (2008) SIRT1 Contributes in Part to Cisplatin Resistance in Cancer Cells by Altering Mitochondrial Metabolism. Mol Cancer Res 6(9): 1499-506. |
| H22 | Hepatoma | Mouse | GeneSilencer siRNA Transfection Reagent | Huang, B., Lei, Z., Zhang, G-M, Li, D., Song, C., Li, B., Liu, Y., Yuan, Y., Unkeless, J., Xiong, H. and Feng, Z-H (2008) SCF-mediated mast cell infiltration and activation exacerbate the inflammation and immunosuppression in tumor microenvironment. Blood 112(4): 1269-79. |
| H22 | Hepatoma | Mouse | GeneSilencer siRNA Transfection Reagent | Huang, B., Zhao, J., Shen, S., Li, H., He, K-L, Shen, G-X, Mayer, L., Unkeless, J., Li, D., Yuan, Y., Zhang, G.-M., Xiong, H. and Feng, Z.-H. (2007) Listeria monocytogenes Promotes Tumor Growth via Tumor Cell Toll-Like Receptor 2 Signaling. Cancer Res. 67(9): p. 4346-4352. |
| H441 | Lung Adenocarcinoma | Human | GeneSilencer siRNA Transfection Reagent | Zhang, Y-A., Nemunaitis, J., Samuel, S.K., Chen, P., Shen, Y. and Tong, A.W. (2006) Antitumor Activity of an Oncolytic Adenovirus-Delivered Oncogene Small Interfering RNA. Cancer Res. 66(19): 9736-9743. |
| H460 | Lung Cancer | Human | GeneSilencer siRNA Transfection Reagent | Ren, J., Shi, M., Liu, R., Yang, Q-H., Johnson, T., Skarnes, W.C. and Du, C. (2005) The Birc6 (Bruce) gene regulates p53 and the mitochondrial pathway of apoptosis and is essential for mouse embryonic development. Proc. Natl. Acad. Sci. USA. 102(3): p. 565-570. |
| H9c2 | Myoblast | Rat | GeneSilencer siRNA Transfection Reagent | Saotome, M., Safiulina, D., Szabadkai, G., Das, S., Fransson, A., Aspenstrom, P., Rizzuto, R. and Hajnoczky, G. (2008) Bidirectional Ca2+-dependent control of mitochondrial dynamics by the Miro GTPase. Proc Natl Acad Sci U S A 105(52): p. 20728. |
| hCMEC/D3 | Brain Microvascular Endothelia | Human | GeneSilencer siRNA Transfection Reagent | Huang, W., Eum, S.Y., Andras, I.E., Hennig, B. and Toborek, M. (2009) PPAR{alpha} and PPAR{gamma} attenuate HIV-induced dysregulation of tight junction proteins by modulations of matrix metalloproteinase and proteasome activities. FASEB J 13 Jan 2009. |
| HCP40 | Colon adenocarcinoma | null | GeneSilencer siRNA Transfection Reagent | Yao, K., Shida, S., Selvakumaran, M., Zimmerman, R., Simon, E., Schick, J., Haas, N.B., Balke, M., Ross, H., Johnson, S.W. and O"Dwyer, P.J. (2005) Macrophage Migration Inhibitory Factor Is a Determinant of Hypoxia-Induced Apoptosis in Colon Cancer Cell Lines. Clin Cancer Res. 11 (20): 7264 - 7272. |
| HCT-116 | Colon Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Wang, J., Rajput, A., Kan, J.L.C., Rose, R., Liu, X., Kuropatwinski, K., Hauser, J., Beko, A., Dominquez, I., Sharratt, E.A., Brattain, L., LeVea, C., Sun, F., Keane, D.M., Gibson, N.W. and Michael G. Brattain (2009) Knockdown of Ron kinase inhibits mutant PI3 kinase and reduces metastasis in human colon carcinoma. J. Biol. Chem. 284 (16) 10912-10922. |
| HCT-116 | Colon Adenocarcinoma | Human | GeneSilencer siRNA Transfection Reagent | Yao, K., Shida, S., Selvakumaran, M., Zimmerman, R., Simon, E., Schick, J., Haas, N.B. Balke, M., Ross, H., Johnson, S.W. and O"Dwyer, P.J. (2005) Macrophage Migration Inhibitory Factor Is a Determinant of Hypoxia-Induced Apoptosis in Colon Cancer Cell Lines. Clin. Can. Res. 11: 7264-7272. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Ahn, S., Nelson, C.D., Garrison, T.R., Miller, W.E. and Lefkowitz, R.J. (2003) Desensitization, internalization, and signaling functions of -arrestins demonstrated by RNA interference. Proc. Natl. Acad. Sci. 100: 1740 - 1744. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Ahn, S., Shenoy, S.K., Wei, H. and Lefkowitz, R.J. (2004) Differential Kinetic and Spatial Patterns of {beta}-Arrestin and G Protein-mediated ERK Activation by the Angiotensin II Receptor. J. Biol. Chem. 279 (34): 35518-35525. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Ahn, S., Wei, H., Garrison, T.R. and Lefkowitz, R.J. (2004) Reciprocal Regulation of Angiotensin Receptor-activated Extracellular Signal-regulated Kinases by (beta)-Arrestins 1 and 2. J. Biol. Chem. 279: 7807-7811. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Khundmiri, S.J., Dean, W.L., McLeish, K.R. and Lederer, E.D. (2005) Parathyroid Hormone-mediated Regulation of Na+-K+-ATPase Requires ERK-dependent Translocation of Protein Kinase C{alpha}. J. Biol. Chem. 280(10): p. 8705-8713. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Kim, J., Ahn, S., Ren, X-R., Whalen, E.J., Reiter, E., Wei, H. and Lefkowitz, R.J. (2005) Functional antagonism of different G protein-coupled receptor kinases for {beta}-arrestin-mediated angiotensin II receptor signaling. Proc. Natl. Acad. Sci.驴102: 1442 - 1447. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Kohout, T.A., Nicholas, S.L., Perry, S.J., Reinhart, G., Junger, S. and Struthers, R. (2004) Differential Desensitization, Receptor Phosphorylation, {beta}-Arrestin Recruitment, and ERK1/2 Activation by the Two Endogenous Ligands for the CC Chemokine Receptor 7. J. Biol. Chem. 279 (22): p. 23214-23222. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Liang, Y., Yu, W., Li, Y., Yan, X., Huang, Q. and Zhu, X. (2004) Nudel functions in membrane traffic mainly through association with Lis1 and cytoplasmic dynein. J. Cell Biol. 164 (4): 557-566. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Ren, X-R., Reiter, E., Ahn, S., Kim, J., Chen, W. and Lefkowitz, R.J. (2005) Different G protein-coupled receptor kinases govern G protein and {beta}-arrestin-mediated signaling of V2 vasopressin receptor. Proc. Natl. Acad. Sci. 102: 1448 - 1453. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Shenoy, S.K., Drake, M.T., Nelson, C.D., Houtz, D.A., Xiao, K., Madabushi, S., Reiter, E., Premont, R.T., Lichtarge, O. and Lefkowitz, R.J. (2006) {beta}-Arrestin-dependent, G Protein-independent ERK1/2 Activation by the {beta}2 Adrenergic Receptor. J. Biol. Chem. 281(2): 1261-1273. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Shikama, Y., Yamada, M., Miyashita, T. (2004) Caspase-8 and caspase-10 activate NF-B through RIP, NIK and IKK kinases. Eur. J. Immun. 33 (7): 1998-2006. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Wei, H., Ahn, S., Barnes, W.G. and Lefkowitz, R.J., (2004) Stable interaction between beta -arrestin2 and AT 1A receptor is required for beta -arrestin2 mediated activation of extracellular signal-regulated kinase 1 and 2. J. Biol. Chem. 279: 48255-48261. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Wei, H., Ahn, S., Shenoy, S.K., Karnik, S.S., Hunyady, L., Luttrell, L.M. and Lefkowitz, R.J. (2003) Independent -arrestin 2 and G protein-mediated pathways for angiotensin II activation of extracellular signal-regulated kinases 1 and 2. Proc. Natl. Acad. Sci. 100: 10782 - 10787. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Wu, J-H, Goswami, R., Kim, L.K., Miller, W.E., Peppel, K. and Freedman, N.J. (2005) The Platelet-derived Growth Factor Receptor-{beta} Phosphorylates and Activates G Protein-coupled Receptor Kinase-2: a Mechanism for Feedback Inhibition. J. Biol. Chem. 280(35): p. 31027-31035. |
| HEK 293T | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Wei, H., Ahn, S., Shenoy, S.K., Karnik, S.S., Hunyady, L., Luttrell, L.M. and Lefkowitz, R.J. (2003) Independent -arrestin 2 and G protein-mediated pathways for angiotensin II activation of extracellular signal-regulated kinases 1 and 2. Proc. Natl. Acad. Sci. 100: 10782 - 10787. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Ahn, S., Kim, J., Hara, M.R., Ren, X. and Lefkowitz, R.J. (2009) β -arrestin2 mediates anti-apoptotic signaling through regulation of bad phosphorylation. J Biol Chem 284(13): 8855-65. |
| HEK-293 | Embryonic Fibroblast | Human | GeneSilencer siRNA Transfection Reagent | Barthet, G., Framery, B., Gaven, F., Pellissier, L., Reiter, E., Claeysen, S., Bockaert, J. and Dumuis, A. (2007) 5-Hydroxytryptamine 4 Receptor Activation of the Extracellular Signal-regulated Kinase Pathway Depends on Src Activation but Not on G Protein or beta-Arrestin Signaling. Mol. Biol. Cell. 18(6): 1979-1991. |
| HEk-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | El-Shewy, H.M., Johnson, K.R., Lee, M-H, Jaffa, A.A., Obeid, L.M. and Luttrell, L.M. (2006) Insulin-like Growth Factors Mediate Heterotrimeric G Protein-dependent ERK1/2 Activation by Transactivating Sphingosine 1-Phosphate Receptors . J. Biol. Chem. 281(42): 31399-31407. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | El-Shewy, H.M., Lee, M-H., Obeid, L.M., Jaffa, A.A. and Luttrell, L.M. (2007) The Insulin-like Growth Factor Type 1 and Insulin-like Growth Factor Type 2/Mannose-6-phosphate Receptors Independently Regulate ERK1/2 Activity in HEK293 Cells. J. Biol. Chem. 282(36): 26150-26157. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Gesty-Palmer, D., Chen, M., Reiter, E., Ahn, S., Nelson, C.D., Wang, S., Eckhardt, A.E., Cowan, C.L., Spurney, R.F., Luttrell, L.M. and Lefkowitz, R.J. (2006) Distinct beta-Arrestin- and G Protein-dependent Pathways for Parathyroid Hormone Receptor-stimulated ERK1/2 Activation. J. Biol. Chem. 281(16): 10856-10864. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | IM Kim, DG Tilley, J Chen, NC Salazar, EJ Whalen, JD Violin, and HA Rockman (2008) Beta-blockers alprenolol and carvedilol stimulate beta-arrestin-mediated EGFR transactivation. Proc Natl Acad Sci 105(38): 14555-60. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Kani, S., Nakayama, E., Yoda, A., Onishi, N., Sougawa, N., Hazaka, Y., Umeda, T., Takeda, K., Ichijo, H., Hamada, Y. and Minami, Y. (2007) Chk2 kinase is required for methylglyoxal-induced G2/M cell-cycle checkpoint arrest: implication of cell-cycle checkpoint regulation in diabetic oxidative stress signaling. Genes Cells. 12(8): 919-928. |
| HEk-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Kara, E., Crepieux, P., Gauthier, C., Martinat, N., Piketty, V., Guillou, F., and Reiter, E. (2006) A Phosphorylation Cluster of Five Serine and Threonine Residues in the C-Terminus of the Follicle-Stimulating Hormone Receptor Is Important for Desensitization But Not for β-Arrestin-Mediated ERK Activation. Mol. Endocrinol. 20(11): p. 3014-3026. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Nelson, C.D., Kovacs, J.J., Nobles, K.N., Whalen, E.J. and Lefkowitz, R.J. (2008) β-Arrestin Scaffolding of Phosphatidylinositol 4-Phosphate 5-Kinase Iα Promotes Agonist-stimulated Sequestration of the {beta}2-Adrenergic Receptor. J. Biol. Chem. 283(30): p. 21093-21101. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Noma, T., Lemaire, A., Prasad, S.V.N., Barki-Harrington, L., Tilley, D.G., Chen, J., Le Corvoisier, P., Violin, J.D., Wei, H., Lefkowitz, R.J. and Rockman, H.A. (2007) β-Arrestin mediated β1-adrenergic receptor transactivation of the EGFR confers cardioprotection. J. Clin. Invest. 117(9): p. 2445-2458. |
| HEk-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Rajagopal, K., Whalen, E.J., Violin, J.D., Stiber, J.A., Rosenberg, P.B., Premont, R.T., Coffman, T.M., Rockman, H.A. and Lefkowitz, R.J. (2006) β-Arrestin2-mediated inotropic effects of the angiotensin II type 1A receptor in isolated cardiac myocytes. PNAS. 103: 16284 - 16289. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Shenoy, S.K., Xiao, K., Venkataramanan, V., Snyder, P.M., Freedman, N.J. and Weissman, A.M. (2008) NEDD4 mediates agonist-dependent ubiquitination, lysosomal targeting and degradation of the beta 2 adrenergic receptor. J. Biol. Chem. 283: 22166 - 22176. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Violin, J.D., DeWire, S.M., Barnes, W.G. and Lefkowitz, R.J. (2006) G Protein-Coupled Receptor Kinase and β-arrestin mediated desensitization of the angiotensin II type 1A receptor elucidated by diacylgylcerol dynamics. J. Biol. Chem. 281: 36411 - 36419. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Bentley, A.M., Normand, G., Hoyt, J. and King, R.W. (2007) Distinct Sequence Elements of Cyclin B1 Promote Localization to Chromatin, Centrosomes, and Kinetochores during Mitosis. Mol. Biol. Cell, Dec 2007; 18: 4847 - 4858. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Cassimeris, L. and Morabito, J. (2004) TOGp, the Human Homolog of XMAP215/Dis1, Is Required for Centrosome Integrity, Spindle Pole Organization, and Bipolar Spindle Assembly. Mol. Biol. Cell 15 (4): 1580-1590. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Chung, J-S., Sato, K., Dougherty, I.I., Cruz, Jr., P.D. and Ariizumi, K. (2007) DC-HIL is a negative regulator of T lymphocyte activation. Blood. 109(10): p. 4320-4327. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Kheifets, V., Bright, R., Inagaki, K., Schechtman, D. and Mochly-Rosen, D. (2006) Protein Kinase C δ}PKC)-Annexin V Interaction: a required step in δ PKC translocation and function. J. Biol. Chem. 281(32): p. 23218-23226. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Layzer, J.M., McCaffrey, A.P., Tanner, A.K., Huang, Zan, Kay, Mark A. and Sullenger, B.A. (2004) In vivo activity of nuclease-resistant siRNAs. RNA 10 (5): p. 766-771. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Pomerening, J.R., Ubersax, J.A. and Ferrell, J.E., Jr. (2008) Rapid Cycling and Precocious Termination of G1 Phase in Cells Expressing CDK1AF. Mol. Biol. Cell, 19: 3426 - 3441. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Shulga, N. and Pastorino, J.G. (2006) Acyl Coenzyme A-binding Protein Augments Bid-induced Mitochondrial Damage and Cell Death by Activating μ-Calpain. J. Biol. Chem. 281(41): 30824-30833. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Witherow, D.S., Garrison, T.R., Miller, W.E. and Robert J. Lefkowitz (2004) {beta}-Arrestin inhibits NF-{kappa}B activity by means of its interaction with the NF-{kappa}B inhibitor I{kappa}B{alpha}. Proc. Natl. Acad. Sci. USA 101: 8603-8607. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Yang, Q-H, Church-Hajduk, R., Ren, J., Newton, M.L. and Du, C. (2003) Omi/HtrA2 catalytic cleavage of inhibitor of apoptosis (IAP) irreversibly inactivates IAPs and facilitates caspase activity in apoptosis. Genes & Dev. 17: 1487 - 1496. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Zhu, S., Wang, W., Clarke, D.C. and Liu, X. (2007) Activation of Mps1 Promotes Transforming Growth Factor-β-independent Smad Signaling. J. Biol Chem. 282 (25) 18327-18338. |
| HepG2 | Hepatocellular Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Pastorino, J.G. and Shulga, N. (2008) TNFalpha can provoke cleavage and activation of sterol regulatory element binding protein in ethanol exposed cells via a caspase dependent pathway that is cholesterol-insensitive. J. Biol. Chem. 283: 25638 - 25649. |
| HL-60 | Peripheral Blood Promyelocytic Leukemia | Human | GeneSilencer siRNA Transfection Reagent | Smirnova, I.V., Kajstura, M., Sawamura, T. and Goligorsky, M.S. (2004) Asymmetric dimethylarginine upregulates LOX-1 in activated macrophages: role in foam cell formation. AJP: Heart 287(2): p. H782-H790 |
| HMEC-1 | Human Microvascular Endothelial Cells | Human | GeneSilencer siRNA Transfection Reagent | Zhou, X., Stuart, A., Dettin, L.E., Rodriguez, G., Hoel, B. and Gallicano G.I. (2004) Desmoplakin is required for microvascular tube formation in culture. J. Cell Sci. 117: 3129-3140. |
| HPAEC | Pulmonary Artery Endothelium | Human | GeneSilencer siRNA Transfection Reagent | Berdyshev, E.V., Gorshkova, I., Skobeleva, A., Bittman, R., Lu, X., Dudek, S.M., Mirzapoiazova, T., Garcia, J.G.N. and Natarajan, V. (2009) FTY720 inhibits ceramide synthases and upregulates dihydrosphingosine-1-phosphate formation in human lung endothelial cells. J. Biol. Chem. 284 (9): 5467-5477. |
| HT29 | Colon Adenocarcinoma | Human | GeneSilencer siRNA Transfection Reagent | Yao, K., Shida, S., Selvakumaran, M., Zimmerman, R., Simon, E., Schick, J., Haas, N.B. Balke, M., Ross, H., Johnson, S.W. and O"Dwyer, P.J. (2005) Macrophage Migration Inhibitory Factor Is a Determinant of Hypoxia-Induced Apoptosis in Colon Cancer Cell Lines. Clin. Can. Res. 11: 7264-7272. |
| HUT-78 | T Cells | Human | GeneSilencer siRNA Transfection Reagent | Maneechotesuwan, K., Xin, Y., Ito, K., Jazrawi, E., Lee, K-Y., Usmani, O.S., Barnes, P.J. and Adcock, I.M. (2007) Regulation of Th2 Cytokine Genes by p38 MAPK-Mediated Phosphorylation of GATA-3. J. Immunol. 178(4): 2491-2498. |
| JAR | Choriocarcinoma | Human | GeneSilencer siRNA Transfection Reagent | Abboud-Jarrous, G., Atzmon, R., Peretz, T., Palermo, C., Gadea, B.B., Joyce, J.A. and Vlodavsky, I. (2008) Cathepsin L Is Responsible for Processing and Activation of Proheparanase through Multiple Cleavages of a Linker Segment. J. Biol. Chem. 283: 18167 - 18176. |
| Jurkat | T-Cell Lymphoma | Human | GeneSilencer siRNA Transfection Reagent | Nguyen, J.T. and Wells, J.A. (2003) Direct activation of the apoptosis machinery as a mechanism to target cancer cells. Proc. Natl. Acad. Sci. 100: 7533 7538. |
| KATOIII | Gastric Epithelium | Human | GeneSilencer siRNA Transfection Reagent | Nagasako, T., Sugiyama, T., Mizushima, T., Miura, Y., Kato, M. and Asaka, M. (2003) Up-regulated Smad5 Mediates Apoptosis of Gastric Epithelial Cells Induced by Helicobacter pylori Infection. J. Biol. Chem: 278: 4821 - 4825. |
| L/Stab-2 | Fibroblast | Mouse | GeneSilencer siRNA Transfection Reagent | Park, S-Y., Kang, K-B., Thapa, N., Kim, S-Y., Lee, S-J. and Kim, I-S. (2008) Requirement of Adaptor Protein GULP during Stabilin-2-mediated Cell Corpse Engulfment. J. Biol. Chem. 283(16): 10593-10600. |
| LNCaP | Prostate Cancer | Human | GeneSilencer siRNA Transfection Reagent | Takayama, K., Tsutsumi, S., Suzuki, T., Horie-Inoue, K., Ikeda, K., Kaneshiro, K. Fujimura, T., Kumagai, J., Urano, T., Sakaki, Y., Shirahige, K., Sasano, H., Takahashi, S., Kitamura, T., Ouchi, Y., Aburatani, H. and Inoue, S. (2009) Amyloid precursor protein is a primary androgen target gene that promotes prostate cancer growth. Cancer Res 1 69(1): p. 137. |
| M2 | Melanoma | Human | GeneSilencer siRNA Transfection Reagent | Scott, M.G.H., Pierotti, V., Storez, H., Lindberg, E., Thuret, A., Muntaner, O., Labbe-Jullie, C., Pitcher, J.A. and Marullo, S. (2006) Cooperative Regulation of Extracellular Signal-Regulated Kinase Activation and Cell Shape Change by Filamin A and β-Arrestins. Mol. & Cell Biol, 26: 3432-3445. |
| McA-RH 7777 | Hepatoma | Rat | GeneSilencer siRNA Transfection Reagent | Pastorino, J.G. and Shulga, N. (2008) Tumor Necrosis Factor-{alpha} Can Provoke Cleavage and Activation of Sterol Regulatory Element-binding Protein in Ethanol-exposed Cells via a Caspase-dependent Pathway That Is Cholesterol Insensitive. J. Biol. Chem. 283: 25638 - 25649. |
| MCF-7 | Breast Adenocarcinoma | Human | GeneSilencer siRNA Transfection Reagent | Ikeda, K., Ogawa, S., Tsukui, T., Horie-Inoue, K., Ouchi, Y., Kato, S., Muramatsu, M. and Inoue, S. (2004) Protein Phosphatase 5 Is a Negative Regulator of Estrogen Receptor-mediated Transcription. Mol. Endocrinol. 18(5): 1131-1143. |
| MDA MB-231 | Breast Cancer | Human | GeneSilencer siRNA Transfection Reagent | Ge, L., Shenoy, S.K., Lefkowitz, R.J. and DeFea, K.A. (2004) Constitutive protease-activated-receptor-2 mediated migration of MDA MB-231 breast cancer cells requires both beta-arrestin-1 and 2. J. Biol. Chem. 279(53): p. 55419-55424. |
| MDA MB-468 | Breast Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Ge, L., Shenoy, S.K., Lefkowitz, R.J. and DeFea, K.A. (2004) Constitutive protease-activated-receptor-2 mediated migration of MDA MB-231 breast cancer cells requires both beta-arrestin-1 and 2. J. Biol. Chem.279(53): p. 55419-55424. |
| MDA-MB-435 | Melanoma | Human | GeneSilencer siRNA Transfection Reagent | Abboud-Jarrous, G., Atzmon, R., Peretz, T., Palermo, C., Gadea, B.B., Joyce, J.A.and Vlodavsky, I. (2008) Cathepsin L Is Responsible for Processing and Activation of Proheparanase through Multiple Cleavages of a Linker Segment. J. Biol. Chem. 283: 18167 - 18176. |
| MDA-MB-468 | Breast Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Zoudilova, M., Kumar, P., Ge, L., Wang, P., Bokoch, G. M. and DeFea, K. A. (2007) β-arrestin-dependent regulation of the cofilin pathway downstream of protease-activated receptor-2. J. Biol. Chem. 282: 20634 - 20646. |
| MG63 | Osteosarcoma | Human | GeneSilencer siRNA Transfection Reagent | Ichikawa, T., Horie-Inoue, K., Ikeda, K., Blumberg, B. and Inoue, S. (2007) Vitamin K2 induces phosphorylation of protein kinase A and expression of novel target genes in osteoblastic cells. J Mol Endocrinol 1 39 (4): 239. |
| MKN 28 | Gastric Epithelium | Human | GeneSilencer siRNA Transfection Reagent | Nagasako, T., Sugiyama, T., Mizushima, T., Miura, Y., Kato, M. and Asaka, M. (2003) Up-regulated Smad5 Mediates Apoptosis of Gastric Epithelial Cells Induced by Helicobacter pylori Infection. J. Biol. Chem.: 278: 4821 - 4825. |
| MKN 45 | Gastric Epithelium | Human | GeneSilencer siRNA Transfection Reagent | Nagasako, T., Sugiyama, T., Mizushima, T., Miura, Y., Kato, M. and Asaka, M. (2003) Up-regulated Smad5 Mediates Apoptosis of Gastric Epithelial Cells Induced by Helicobacter pylori Infection. J. Biol. Chem.: 278: 4821 - 4825. |
| MV-4-11 | Biophenotypic B Myelomonocytic Leukemia | Human | GeneSilencer siRNA Transfection Reagent | Yang, X., Liu, L., Sternberg, D., Tang, L., Galinsky, I., DeAngelo, D. and Stone, R. (2005) The FLT3 Internal Tandem Duplication Mutation Prevents Apoptosis in Interleukin-3-Deprived BaF3 Cells Due to Protein Kinase A and Ribosomal S6 Kinase 1-Mediated BAD Phosphorylation at Serine 112. Cancer Res. 65(16): p. 7338-7347. |
| Neuro2a | Neuroblastoma | Mouse | GeneSilencer siRNA Transfection Reagent | Bertling, E., Hotulainen, P., Mattila, P.K., Matilainen, T., Salminen, M., and Lappalainen, P. (2004) Cyclase-associated-protein 1 (CAP1) promotes cofilin-induced actin dynamics in mammalian nonmuscle cells. Mol. Biol. Cell 15 (5): p. 2324-2334. |
| NIH-3T3 | Fibroblast | Mouse | GeneSilencer siRNA Transfection Reagent | Bertling, E., Hotulainen, P., Mattila, P.K., Matilainen, T., Salminen, M., and Lappalainen, P. (2004) Cyclase-associated-protein 1 (CAP1) promotes cofilin-induced actin dynamics in mammalian nonmuscle cells. Mol. Biol. Cell 15 (5): 2324-2334. |
| NIH-3T3 | Fibroblast | Mouse | GeneSilencer siRNA Transfection Reagent | Hotulainen, P., Paunola, E., Vartiainen, M.K. and Lappalainen, P. (2005) Actin-depolymerizing Factor and Cofilin-1 Play Overlapping Roles in Promoting Rapid F-Actin Depolymerization in Mammalian Nonmuscle Cells. Mol. Biol. Cell. 16(2): p. 649-664. |
| NIH-3T3 | Fibroblast | Mouse | GeneSilencer siRNA Transfection Reagent | Nomachi, A., Nishita, M., Inaba, D., Enomoto, M., Hamasaki, M. and Minami, Y. (2008) Receptor Tyrosine Kinase Ror2 Mediates Wnt5a-induced Polarized Cell Migration by Activating c-Jun N-terminal Kinase via Actin-binding Protein Filamin A. J. Biol. Chem. 283(41): 27973-27981. |
| OK | Kidney | Opossum | GeneSilencer siRNA Transfection Reagent | Khundmiri, S.J., Ameen, M., Delamere, N.A. and Lederer, E.D. (2008) PTH-mediated regulation of Na+ -K+ -ATPase requires Src kinase-dependent ERK Phosphorylation. Am J Physiol Renal Physiol. 295(2): F426-F437. |
| OK | Kidney | Opossum | GeneSilencer siRNA Transfection Reagent | Khundmiri, S.J., Dean, W.L., McLeish, K.R. and Lederer, E.D. (2005) Parathyroid Hormone-mediated Regulation of Na+-K+-ATPase Requires ERK-dependent Translocation of Protein Kinase C{alpha}. J. Biol. Chem. 280(10): p. 8705-8713. |
| PC-12 | Pheochromocytoma | Rat | GeneSilencer siRNA Transfection Reagent | de Barry, J., Janoshazi, A., Luc Dupont, J., Procksch, O., Chasserot-Golaz, S., Jeromin, A. and Vitale, N. (2006) Functional Implication of Neuronal Calcium Sensor-1 and Phosphoinositol 4-Kinase-β Interaction in Regulated Exocytosis of PC12 Cells. J. Biol Chem. 281(26): 18098-18111. |
| Primary | Cortical Neurons | Mouse | GeneSilencer siRNA Transfection Reagent | Aarts, M., Lihara, K., Wei, W-L, Xiong, Z-G, Arundine, M., Cerwinski, W., MacDonald, J.F., Tymianski, M. (2003) A Key Role for TRPM7 Channels in Anoxic Neuronal Death. Cell 115: 863. |
| Primary | Cortical Neurons | Mouse | GeneSilencer siRNA Transfection Reagent | Aleyasin, H., Cregan, S.P., Iyirhiaro, G., OHare, M.J., Callaghan, S.M., Slack, R.S. and Park, D.S. (2004) Nuclear Factor-B Modulates the p53 Response in Neurons Exposed to DNA Damage. J. Neurosci. 24 (12): 2963-2973. |
| Primary | Hippocampal Neurons | Rat | GeneSilencer siRNA Transfection Reagent | Amaral, M.D. and Pozzo-Miller, L. (2007) TRPC3 Channels Are Necessary for Brain-Derived Neurotrophic Factor to Activate a Nonselective Cationic Current and to Induce Dendritic Spine Formation. J. Neurosci. 27(19): p. 5179-5189. |
| Primary | Macrophage | Human | GeneSilencer siRNA Transfection Reagent | Asmis, R., Wang, Y., Xu, L., Kisgati, M., Begley, J.G. and驴Mieyal, J.J. (2005) A novel thiol oxidation-based mechanism for adriamycin-induced cell injury in human macrophages. FASEB J. published 13 September 2005, 10.1096/fj.04-2991fje. |
| Primary | Pulmonary Artery Endothelium | Human | GeneSilencer siRNA Transfection Reagent | Birukova, A.A., Chatchavalvanich, S., Rios, A., Kawkitinarong, K., Garcia, J.G.N. and Birukov, K.G. (2006) Differential Regulation of Pulmonary Endothelial Monolayer Integrity by Varying Degrees of Cyclic Stretch. Am. J. Pathol. 168: 1749 - 1761. |
| Primary | Cortical Neurons | Mouse | GeneSilencer siRNA Transfection Reagent | Burkhalter, J., Fiumelli, H., Erickson, J.D. and Martin, J-L. (2007) A critical role for system a amino acid transport in the regulation of dendritic development by BDNF. J Biol Chem 282: 5152 - 5159. |
| Primary | Dendritic Cells (Bone Marrow) | Mouse | GeneSilencer siRNA Transfection Reagent | Chung, J-S, Sato, K., Dougherty, I.I., Cruz, Jr., P.D. and Ariizumi, K. (2007) DC-HIL is a negative regulator of T lymphocyte activation. Blood. 109(10): p. 4320-4327. |
| Primary | Cortical Neurons | Mouse | GeneSilencer siRNA Transfection Reagent | Cui, H., Hayashi, A., Sun, H-S, Belmares, M.P., Cobey, C., Phan, T., Schweizer, J., Salter, M.W., Wang, Y.T., Tasker, R.A., Garman, D., Rabinowitz, J., Lu, P.S. and Tymianski, M. (2007) PDZ Protein Interactions Underlying NMDA Receptor-Mediated Excitotoxicity and Neuroprotection by PSD-95 Inhibitors. J. Neurosci. 27(37): 9901-9915. |
| Primary | Coronary Artery Endothelial | Human HCAEC | GeneSilencer siRNA Transfection Reagent | Dandapat, A., Hu, C., Sun, L. and Mehta, J.L. (2007) Small Concentrations of oxLDL Induce Capillary Tube Formation From Endothelial Cells via LOX-1 Dependent Redox-Sensitive Pathway. Arterioscler Thromb Vasc Biol. |
| Primary | Peritoneal Macrophage | Mouse | GeneSilencer siRNA Transfection Reagent | de Beer, M.C., Zhao, Z., Webb, N.R., van der Westhuyzen, D.R. and de Villiers, W.J.S. (2003) Lack of a direct role for macrosialin in oxidized LDL metabolism J. Lipid Res. 44: 674 - 685. |
| Primary | Dorsal Root Ganglion Neurons | Mouse | GeneSilencer siRNA Transfection Reagent | Haruhisa Higuchi, Toshihide Yamashita, Hideki Yoshikawa, and Masaya Tohyama (2003) Functional inhibition of the p75 receptor using a small interfering RNA. Biochem. & Biophys. Res. Comm. 301: 804809. |
| Primary | Retinal Ganglion | Rat | GeneSilencer siRNA Transfection Reagent | Hayashi, H., Campenot, R.B., Vance, D.E. and Vance, J.E. (2007) Apolipoprotein E-Containing Lipoproteins Protect Neurons from Apoptosis via a Signaling Pathway Involving Low-Density Lipoprotein Receptor-Related Protein-1. J. Neurosci. 27(8): 1933-1941. |
| Primary | Dorsal Root Ganglion | Rat | GeneSilencer siRNA Transfection Reagent | Hengst, U. Cox, L.J., Macosko, E.Z. and Jaffrey, S.R. (2006) Functional and Selective RNA Interference in Developing Axons and Growth Cones. J. Neurosci. 26(21): 5727-5732. |
| Primary | Schwann Cells | Mouse | GeneSilencer siRNA Transfection Reagent | Higuchi, H., Yamashita, T., Yoshikawa, H. and Tohyama, M. (2003) Functional inhibition of the p75 receptor using a small interfering RNA. Biochem. & Biophys. Res. Comm. 301: 804809. |
| Primary | Cardiac Fibroblast | Mouse | GeneSilencer siRNA Transfection Reagent | Hu, C., Dandapat, A., Sun, L., Khan, J.A., Liu, Y., Hermonat, P.L. and Mehta, J.L. (2008) Regulation of TGFbeta 1-mediated collagen formation by LOX-1: Studies based on forced over-expression of TGFbeta 1 in wild-type and LOX-1 knockout mice cardiac fibroblasts. J. Biol. Chem. 283 (16) 10226-10231. |
| Primary | Cardiac Myocytes (Neonatal) | Rat | GeneSilencer siRNA Transfection Reagent | Juhaszova, M., Zorov, D.B., Kim, S-H, Pepe, S., Fu, O., Fishbein, K.W., Ziman, B.D., Wang, S., Ytrehus, K., Antos, C.L., Olson, E.N. and Sollott, S.J. (2004) Glycogen synthase kinase-3{beta} mediates convergence of protection signaling to inhibit the mitochondrial permeability transition pore. J. Clin. Invest. 113(11): p. 1535-1549. |
| Primary | Lung Microvascular Endothelial Cells | Human | GeneSilencer siRNA Transfection Reagent | Kolosova, I.A., Ma, S-F, Adyshev, D.M., Wang, P., Ohba, M., Natarajan, V., Garcia, J.G.N. and Verin, A.D. (2004) Role of CPI-17 in the regulation of endothelial cytoskeleton AJP: Lung. 287(5): L970. |
| Primary | Aortic Muscle Cells | Rat | GeneSilencer siRNA Transfection Reagent | Lake, A.C. & Castellot, J.J. (2003) CCN5 modulates the antiproliferative effect of heparin and regulates cell motility in vascular smooth muscle cells. Cell Comm. & Signaling 1: 5. |
| Primary | Dendritic Cells | Mouse | GeneSilencer siRNA Transfection Reagent | Li, M., Qian, H., Ichim, T.E., Ge, W-W, Popov, I.A., Rycerz, K., Neu, J., White, D., Zhong, R., Min, W-P. (2004) Induction of RNA Interference in Dendritic Cells. Immunologic Research 30 (2) 215-230. |
| Primary | Dendritic Cells | Mouse | GeneSilencer siRNA Transfection Reagent | Li, M., Zhang, X., Zheng, X., Lian, D., Zhang, Z-X., Ge, W., Yang, J., Vladau, C., Suzuki, M., Chen, D., Zhong, R., Garcia, B., Jevnikar, A.M. and Min, W-P (2007) Immune Modulation and Tolerance Induction by RelB-Silenced Dendritic Cells through RNA Interference. J. Immunol. 178(9): p. 5480-5487. |
| Primary | Dendritic Cells | Mouse | GeneSilencer siRNA Transfection Reagent | Liu, G., Ng, H., Akasaki, Y., Yuan, X., Ehtesham, M., Yin, D., Black , K.L. and Yu, J.S. (2004) Small interference RNA modulation of IL-10 in human monocyte-derived dendritic cells enhances the Th1 response. Eur. J. Immunol. 34: 1680-1687. |
| Primary | Cerebellar Neurons | Rat | GeneSilencer siRNA Transfection Reagent | Numakawa, T., Nakayama, H., Suzuki, S., Kubo, T., Nara, F., Numakawa, Y., Yokomaku, D., Araki, T., Ishimoto, T., Ogura, A. and Taguchi, T. (2003) Nerve growth factor-induced glutamate release is via p75 receptor, ceramide and Ca2+ from ryanodine receptor in developing cerebellar neurons. J. Biol. Chem: 278: 41259-41269. |
| Primary | Osteoblast | Mouse | GeneSilencer siRNA Transfection Reagent | Ohyama, Y., Nifuji, A., Maeda, Y., Amagasa, T. and Noda, M. (2004) Spaciotemporal Association and Bone Morphogenetic Protein Regulation of Sclerostin and Osterix Expression during Embryonic Osteogenesis. Endocrinology. 145(10): p. 4685-4692. |
| Primary | HMVEC | Human | GeneSilencer siRNA Transfection Reagent | Radu Stefanescu, Dustin Bassett, Rozbeh Modarresi, Francisco Santiago, Mohamad Fakruddin, and Jeffrey Laurence (2008) Synergistic interactions between interferon-{gamma} and TRAIL modulate c-FLIP in endothelial cells, mediating their lineage-specific sensitivity to thrombotic thrombocytopenic purpura plasma-associated apoptosis. Blood 112: 340 - 349. |
| Primary | Cardiac Myocytes | Feline | GeneSilencer siRNA Transfection Reagent | Ramabadran, R. S., Chancey, A., Vallejo, J.G., Barger, P.M., Sivasubramanian, N. and Mann, D.L. (2008) Targeted Gene Silencing of Tumor Necrosis Factor Attenuates the Negative Inotropic Effects of Lipopolysaccharide in Isolated Contracting Cardiac Myocytes. Tex Heart Inst J. 35(1): 16-21. |
| Primary | T-Cells (CD3+) | Human | GeneSilencer siRNA Transfection Reagent | Samten, B., Howard, S.T., Weis, S.E., Wu, S., Shams, H., Townsend, J.C., Safi, H. and Barnes, P.F. (2005) Cyclic AMP Response Element-Binding Protein Positively Regulates Production of IFN-{gamma} by T Cells in Response to a Microbial Pathogen. J. Immunol. 174(10): p. 6357-6363. |
| Primary | Cortical Neurons | Mouse | GeneSilencer siRNA Transfection Reagent | Sato, S., Xu, J., Okuyama, S., Martinez, LB., Walsh, S.M, Jacobsen, M.T, Swan, R.J., Schlautman, J.D., Ciborowski, P. and Ikezu, T. (2008) Spatial learning impairment, enhanced CDK5/p35 activity, and downregulation of NMDA receptor expression in transgenic mice expressing tau-tubulin kinase 1. J Neurosci 28(53): 14511. |
| Primary | Dendritic Cells | Human | GeneSilencer siRNA Transfection Reagent | Smith, A.L., Ganesh, L., Leung, K., Jongstra-Bilen, J., Jongstra, J. and Nabel, G.J. (2007) Leukocyte-specific protein 1 interacts with DC-SIGN and mediates transport of HIV to the proteasome in dendritic cells. J. Exp. Med. 204(2): p. 421-430. |
| Primary | Cortical Neurons | Mouse | GeneSilencer siRNA Transfection Reagent | Supnet, C., Grant, J., Kong, H., Westaway, D. and Mayne, M. (2006) Amyloid β-(1-42) Increases Ryanodine Receptor-3 Expression and Function in TgCRND8 Mice. J. Biol. Chem. 281: 38440 - 38447. |
| Primary | Aveolar Type II Cells | Mouse | GeneSilencer siRNA Transfection Reagent | Ueno, T., Linder, S., Na, C-L, Rice, W.R., Johansson, J., and Weaver, T.E. (2004) Processing of Pulmonary Surfactant Protein B by Napsin and Cathepsin H. J. Biol. Chem. 279: 16178-16184. |
| Primary | Macrophage | Mouse | GeneSilencer siRNA Transfection Reagent | Wang, Y., Chen, T., Han, C., He, D., Liu, H., An, H., Cai, Z. and Cao, X. (2007) Lysosome-associated small Rab GTPase Rab7b negatively regulates TLR4 signaling in macrophages by promoting lysosomal degradation of TLR4. Blood 110: 962 - 971. |
| Primary | Dorsal Root Ganglion | Rat | GeneSilencer siRNA Transfection Reagent | Wu, K.Y., Hengst, U., Cox, L.J., Macosko, E.Z., Jeromin, A., Urquhart, E.R., and Jaffrey, S.R. (2005) Local translation of RhoA regulates growth cone collapse. Nature 436: 1020-1024. |
| Primary | Lacrimal Gland Acinar Cells | Rabbit | GeneSilencer siRNA Transfection Reagent | Xie, J., Chiang, L., Contreras, J., Wu, K., Garner, J.A., Medina-Kauwe, L. and Hamm-Alvarez, S.F. (2006) Novel Fiber-Dependent Entry Mechanism for Adenovirus Serotype 5 in Lacrimal Acini. J. Virol. 80(23): 11833 - 11851. |
| Primary | Bone Marrow | Mouse | GeneSilencer siRNA Transfection Reagent | Yang, R., Cai, Z., Zhang, Y., Yutzy IV, W.H., Roby, K.F. and Roden, R.B.S. (2006) CD80 in Immune Suppression by Mouse Ovarian Carcinoma驴Associated Gr-1+CD11b+ Myeloid Cells. Cancer Res 66 (13): 6807 - 6815. |
| Primary | Dendritic Cells | Mouse | GeneSilencer siRNA Transfection Reagent | Zheng, X., Koropatnick, J., Li, M., Zhang, X., Ling, F., Ren, X., Hao, X., Sun, H., Vladau, C., Franek, J.A., Feng, B., Urquhart, B.L., Zhong, R., Freeman, D.J., Garcia, B. and Min, W-P. (2006) Reinstalling Antitumor Immunity by Inhibiting Tumor-Derived Immunosuppressive Molecule IDO through RNA Interference. J. Immunol. 177(8): 5639-5646. |
| Primary | Pulmonary Artery Endothelium | Human | GeneSilencer siRNA Transfection Reagent | Zhuowei Li, Xhevahire Hyseni, Jacqueline D. Carter, Joleen M. Soukup, Lisa A. Dailey, and Yuh-Chin T. Huang (2006) Pollutant particles enhanced H2O2 production from NAD(P)H oxidase and mitochondria in human pulmonary artery endothelial cells. Am J Physiol Cell Physiol 291: C357 - C365. |
| Primary HPAEC | Pulmonary Artery Endothelium | Human | GeneSilencer siRNA Transfection Reagent | Rentsendorj, O., Mirzapoiazova, T., Adyshev, D., Servinsky, L.E., Renne, T., Verin, A.D. and Pearse, D.B. (2008) Role of vasodilator-stimulated phosphoprotein in cGMP-mediated protection of human pulmonary artery endothelial barrier function. Am J Physiol Lung Cell Mol Physiol. 294(4): L686 -L697. |
| RAW 264.7 | Macrophage | Mouse | GeneSilencer siRNA Transfection Reagent | de Beer, M.C., Zhao, Z., Webb, N.R., van der Westhuyzen, D.R. and de Villiers, W.J.S. (2003) Lack of a direct role for macrosialin in oxidized LDL metabolism. J. Lipid Res. 44: 674 - 685. |
| RAW 264.7 | Macrophage | Mouse | GeneSilencer siRNA Transfection Reagent | Liu, X., Yao, M., Li, N., Wang, C., Zheng, Y. and Cao, X. (2008) CaMKII promotes TLR-triggered proinflammatory cytokine and type I interferon production by directly binding and activating TAK1 and IRF3 in macrophages. Blood. 112(13): 4961-4970. |
| Renca | Renal Cell Carcinoma | Mouse | GeneSilencer siRNA Transfection Reagent | Ogushi, T., Takahashi, S., Takeuchi, T., Urano, T., Horie-Inoue, K., Kumagai, J., Kitamura, T., Ouchi, Y., Muramatsu, M. and Inoue, S. (2005) Estrogen Receptor-Binding Fragment-Associated Antigen 9 Is a Tumor-Promoting and Prognostic Factor for Renal Cell Carcinoma. Cancer Res. 65(9): p. 3700-3706. |
| SaOs2 | Osteosarcoma | Human | GeneSilencer siRNA Transfection Reagent | Chu, F.,Chou, P.M., Zheng, X., Mirkin, B.L. and Rebbaa, A.驴(2005)驴Control of Multidrug Resistance Gene mdr1 and Cancer Resistance to Chemotherapy by the Longevity Gene sirt1. Cancer Res.65 (22): p. 10183-10187. |
| SaOS2 | Osteosarcoma | Human | GeneSilencer siRNA Transfection Reagent | Zheng, X., Chu, F., Chou, P.M., Gallati, C., Dier, U., Mirkin, B.L., Mousa, S.A. and Rebbaa, A. (2009) Cathepsin L inhibition suppresses drug resistance in vitro and in vivo: a putative mechanism. Am J Physiol Cell Physiol. 296(1): C65-C74. |
| Saos-2 | Osteosarcoma | Human | GeneSilencer siRNA Transfection Reagent | Zheng, X., Chou, P.M., Mirkin, B.L. and Rebbaa, A. (2004) Senescence-initiated Reversal of Drug Resistance: Specific Role of Cathepsin L. Cancer Res. 64: 1773 - 1780. |
| SCCH196 | Sarcoma | Human | GeneSilencer siRNA Transfection Reagent | Takahashi, A., Higashino, F., Aoyagi, M., Yoshida, K., Itoh, M., Kyo, S., Ohno, T., Taira, T., Ariga, H., Nakajima, K., Hatta, M., Kobayashi, M., Sano, H., Kohgo, T. and Shindoh, M., (2003) EWS/ETS Fusions Activate Telomerase in Ewings Tumors. Cancer Res. 63: 8338-8344. |
| SNB19 | Glioblastoma | Human | GeneSilencer siRNA Transfection Reagent | Song, S.W., Fuller, G.N., Zheng, H. and Zhang, W. (2005) Inactivation of the Invasion Inhibitory Gene IIp45 by Alternative Splicing in Gliomas. Cancer Res. 65(9): p. 3562-3567. |
| SW480 | Colon Adenocarcinoma | Human | GeneSilencer siRNA Transfection Reagent | Zhu, S., Wang, W., Clarke, D.C. and Liu, X. (2007) Activation of Mps1 Promotes Transforming Growth Factor-β-independent Smad Signaling. J. Biol. Chem. 282(25): 18327-18338. |
| U20S | Osteosarcoma | Human | GeneSilencer siRNA Transfection Reagent | Hotulainen P. and Lappalainen, P. (2006) Stress fibers are generated by two distinct actin assembly mechanisms in motile cells J. Cell Biol. 173(3): 383-394. |
蚂蚁淘电商平台
ebiomall.com
ebiomall.com
公司简介
蚂蚁淘（www.ebiomall.cn）是中国大陆目前唯一的生物医疗科研用品B2B跨境交易平台，
该平台由多位经验丰富的生物人和IT人负责运营。蚂蚁淘B2B模式是指客户有采购意向后在蚂蚁
淘搜索全球供应信息，找到合适的产品后在蚂蚁淘下单，然后蚂蚁淘的海外买手进行跨境采购、
运输到中国口岸，最后由蚂蚁淘国内团队报关运输给客户...
蚂蚁淘承诺
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蚂蚁淘所有产品都是自运营的，我们已经跟国外多家厂方建立品牌推广合作关系, 获得对方的支持和授权； 同时客户可以通过订单详情查看到货物从厂方至客户的所有流程， 确保货物的来源； 正规报关，提供13%增值税发票。
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蚂蚁淘的价格是真实透明的，并且具有很大的价格优势，不需要繁杂的询价比价； 报价单与合同可以直接在线生成或打印；就像在京东购物一样， 您的鼠标点击几 次即完成在蚂蚁淘的采购，订单详情会告诉您所有进程。
售后申请： 耐心讲解 优质服务
蚂蚁淘提供的产品在使用过程中如因产品质量问题有售后需求时， 您可通过我的订单提交您的“申请售后”， 蚂蚁淘产品顾问会第一时间为您处理， 在售后服务过程中如遇到问题也可致电蚂蚁淘客服热线：4000-520-616。
2021-09-07
最大超声功率输出：250W
主机尺寸：270*200*140mm
控制模式：自动、手动可切换(可选配脚踏开关
温度控制：超声保护报警功能
脉冲器：闭循环0.1-99s可调 开循环0.1-99S可调 查看更多
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2017-01-19
超声波细胞破碎仪（粉碎机）是一种利用强超声在液体中产生空化效应，对物质进行超声处理的多功能、多用途的仪器，能用于多种动植物细胞、病毒细胞的破碎，同时可用来乳化、分离、匀化、提取、消泡、清洗及加速化学反应等等。被广泛应用于生物化学、微生物学、药物化学、表面化学、物理学、动物学等领域。本产品为新款触摸屏系列，外形美观、功能齐全、操作简单、可扩展性强且价格合理。型号FC-650NFC-1000NFC-1800NFC-3000N频率KHz20-... 查看更多
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2021-09-10
频率：19.5-20.5KHz
功率： 1200W
随机变幅杆：Φ20
可选配变幅杆：Φ15、25
破碎容量：50-1000ml
占空比：1-99% 查看更多
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2021-09-14
[db:简介] 查看更多
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2017-12-19
液晶型超声波细胞粉碎机是由上海朗赋实业有限公司代理或销售的0品牌的仪器，产品来源于中国。上海朗赋实业有限公司是中国最权威的液晶型超声波细胞粉碎机销售服务商之一，在上海等地方销售液晶型超声波细胞粉碎机已经多年。同时，生物在线为您提供众多企业液晶型超声波细胞粉碎机仪器产品及图片，以便挑选到性价比高，合适的液晶型超声波细胞粉碎机产品 查看更多
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2021-09-08
频率：20-25KHz
功率：900W
随机变幅杆:Φ6
可选配变幅杆：Φ2、3、10、12、15
破碎容量:0.5-600ml
占空比：0.1-99.9% 查看更多
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2021-08-31
工作频率范围：20-25KHz，频率自动跟踪。
发生器输出功率：400W(max)
随机变幅杆：Φ10
工作电压：220V±5% 查看更多
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2021-07-18
胸膜粘连术是治疗恶性胸水的主要方法之一，但如果肺已受恶性肿瘤侵犯导致肺萎陷不能复张，则适宜留置胸腔导管。因为目前还没有很好的方法能在胸水穿刺引流前诊断萎陷肺，为确定治疗方案，患者往往需要经受多次胸腔穿刺。 超声诊断中有很多分析组织运动的模式，如运动模式（M Mode），能定量组织移位，而更高级的弹性图像通过压力的表现形式能定量组织变形。 为 查看更多
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2021-09-20
[db:简介] 查看更多
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2017-01-19
产品概述： 本仪器是一种利用强超声在液体中产生空化效应，对物质进行超声处理的多功能、多用途的仪器，能用于多种动植物细胞、病毒细胞的破碎，同时可用来乳化、分离、匀化、提取、消泡、清洗及加速化学反应等等。被广泛应用于生物化学、微生物学、药物化学、表面化学、物理学、动物学等领域。手持式超声波破碎仪外观美，性能可靠。仪器采用大屏幕显示，中央微机集中控制，超声时间，功率任意设定，样品温度检测显示、频率微机跟踪、故障自动报警。所有功能都显示在液... 查看更多
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2017-10-19
手持式细胞粉碎机（破碎仪）是由上海朗赋实业有限公司代理或销售的0品牌的仪器，产品来源于中国。上海朗赋实业有限公司是中国最权威的手持式细胞粉碎机（破碎仪）销售服务商之一，在上海等地方销售手持式细胞粉碎机（破碎仪）已经多年。同时，生物在线为您提供众多企业手持式细胞粉碎机（破碎仪）仪器产品及图片，以便挑选到性价比高，合适的手持式细胞粉碎机（破碎仪）产品 查看更多
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2021-09-11
[db:简介] 查看更多
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常见问题
蚂蚁淘所售产品均为正品吗？
蚂蚁淘的创始人兼CEO是钟定松先生，具有十年的从业经验，在业界享有良好的口碑；
Ebiomall是跨境直采平台，我们直接从厂家采购，自己的团队负责国际物流和清关，中间没有第三方，蚂蚁淘承诺所售产品仅为正品，假一罚十。
下单后可以修改订单吗？
未确认状态的订单可以修改，打开“订单详情”页面，点击右上角的“修改订单”即可，若已审核确定，则订单无法修改。
商品几天可以发货？
现货产品付款审核后即可发货,大部分期货产品在3周左右即可到货,提供时必达服务的产品订单审核十天内即可发货。
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一般情况下，退货处理周期为客户收到产品一个月内（以快递公司显示签收时间为准），包装规格、数量、品种不符，外观毁损、短缺或缺陷，请在收到货24小时内申请退换货；特殊商品以合同条款为准。
商品咨询
美国Branson必能信超声波细胞破碎仪/S250D/S450D钦烁实验器材...123
xbnlkd2021-07-21
大家好，我做蛋白质组学，也就是双向电泳，想买一台超声破碎仪，大家觉得什么牌子的好啊？？美国BRANSON超声波细胞破碎仪怎么样啊？？？
超声波破碎仪功率多少才能破碎细胞_问答详情_蚂蚁淘,【正品极速...123
棕油2722017-10-21
探头位置没放好:探头一定要接近底部，约1cm。功率根据仪器不同会有所不同，但你可以观察液面，有波动但不要太剧烈就好。
超声破碎的条件不能一概而论，要看你的实验要求，有的是细菌破碎，有的是对组织细胞进行破碎，要掌握好功率和每次超声时间，功率大时，每次超声时间可缩短，不能让温度升高，必要时在冰浴条件下进行超声破碎。至于每次超声时是否起泡沫到不是关键的问题，这与你超声的量明显有关。
超声破碎的条件不能一概而论，要看你的实验要求，有的是细菌破碎，有的是对组织细胞进行破碎，要掌握好功率和每次超声时间，功率大时，每次超声时间可缩短，不能让温度升高，必要时在冰浴条件下进行超声破碎。至于每次超声时是否起泡沫到不是关键的问题，这与你超声的量明显有关。
超声波细胞破碎仪按功率分类123
霸道HVet72021-08-21
超声波设备与其他能量转换设备（如马达、发动机等）一样，设备的输入功率为电学端功率+机械端功率，而其功率的消耗大部分在机械端（力学端），因此机械端的特性就决定了整套超声波系统的功率。对于超声波功率来说，有标称功率和实际功率
【资源】(分享学习)超声波破碎细胞的原理及超声细胞破碎仪的用途 ...123
lrb20012021-08-23
超声波是物质介质中的一种弹性机械波，它是一种波动形式，因此它可以用于探测人体的生理及病理信息，既诊断超声。同时，它又是一种能量形式，当达到一定剂量的超声在生物体内传播时，通过它们之间的相互作用.超声波是物质介质中的一种弹性机械波，它是一种波动形式，因此它可以用于探测人体的生理及病理信息，既诊断超声。同时，它又是一种能量形式，当达到一定剂量的超声在生物体内传播时，通过它们之间的相互作用，能引起生物体的功能和结构发生变化，即超声生物效应。超声对细胞的作用主要有热效应，空化效应和机械效应。热效应是当超声在介质中传播时，摩擦力阻碍了由超声引起的分子震动，使部分能量转化为局部高热（42-43℃），因为正常组织的临界致死温度为45.7℃，而肿瘤组织比正常组织敏感性高，故在此温度下肿瘤细胞的代谢发生障碍，DNA、RNA、蛋白质合成受到影响，从而杀伤癌细胞而正常组织不受影响。空化效应是在超声照射下，生物体内形成空泡，随着空泡震动和其猛烈的聚爆而产生出机械剪切压力和动荡，使肿瘤出血、组织瓦解以致坏死。另外，空化泡破裂时产生瞬时高温（约5000℃）、高压（可达500×104Pa），可使水蒸气热解离产生.OH自由基和.H原子，由.OH自由基和.H原子引起的氧化还原反应可导致多聚物降解、酶失活、脂质过氧化和细胞杀伤。机械效应是超声的原发效应，超声波在传播过程中介质质点交替地压缩与伸张构成了压力变化，引起细胞结构损伤。杀伤作用的强弱与超声的频率和强度密切相关。
超声波细胞破碎仪的原理并不是太神秘、太复杂。简单说就是将电能通过换能器转换为声能，这种能量通过液体介质(如水)而变成一个个密集的小气泡，这些小气泡迅速炸裂，产生的象小***一样的能量，从而起到破碎细胞等物质的作用(俗称“空化效应”)。
超声波细胞粉碎机是利用一种超声波在液体中产生空化效应的多功能、多用途的仪器；它能用于各种动植物细胞、病毒细胞、细菌及组织的破碎，也可用于各类无机物质的破碎重组，同时可用来乳化、分离、匀化、提取、消泡清洗及加速化学反应等。超声波细胞粉碎机有广泛的用途，如:
1、超声波提取生物纳米（超声波化学合成法）
超声波化学反应中，起关键作用的是声波的空化效应，在超声波的辐照过程中，在液体里将发生空化气泡的形成，长大和崩灭，当空化气泡崩灭时产生一个覆盖着的强压力脉冲，产生许多独特的性质，例如产生高达5000K的高温，大于200Mpa的压力，这就是超声波化学合成的能量来源，利用这些能量能在一些特殊粉末表面合成出纳米粒子。
2、超声波制药（1）注射用医药物质的分散——将磷脂类与胆固醇混合用适当方法与药物混合在水溶液中，经超声分散，可以得到更小粒子供静脉注射。
（2）草药提取——利用超声分散破坏植物组织，加速溶剂穿透组织作用，提高中草药有效成分提取率。如金鸡纳树皮中全部生物碱用一般方法侵出需5小时以上，采用超声分散只要半小时即可完成。
（3）制备混悬剂——在超声空化和强烈搅拌下，将一种固体药物分散在含有表面活性剂的水溶液中，可以形成1um左右口服或静脉注射混悬剂。例“静注喜树碱混悬剂”“肝脏造影剂”、“硫酸钡混悬剂”。
（4）制备疫苗——将细胞或病菌借助于超声分散将其杀死以后，再用适当方法制成疫苗。
3、超声波对化妆品的分散
为了更进一步提取药物精华和粒子微细化，并节约生产成本，达到分散、乳化效果，使化妆品更深入渗透到肌肤里层，让肌肤很好的吸收，发挥药物的效力和作用，采用超声波乳化可达到非常理想的效果。采用超声分散，则不需要使用乳化剂，就能使蜡及石蜡乳化、化妆水等油的微粒子分散。石腊在水中分散的粒子直径可达1um以下。
4、超声波对酒的醇化—催陈技术
一瓶美酒以它的酒味醇厚，绵软柔和、芳香浓郁为人青睐，人们常用陈年老酒来形容酒的珍贵，一瓶上世纪的陈酒，标价几万元，其价格的含义在于时间的存放上。酒的主要控制因素是化学变化即酸的形成，并进一步酯化，酯参与乙醇和水的缔合。刚出厂的酒含有戍醇，有辛辣味，这种气味要经过很长时间才能化解，这个缓慢变化称酒的醇化。用功率1.6KW，频率17.5-22KHZ的超声波处理5-10min，可使酒的老熟时间缩短1/3到1/2。
因此超声波细胞粉碎机可以并且已经被广泛用于生物化学、微生物学药理学、物理学、动物学、农学、医学、制药等领域的教学、科研、生产。
超声波细胞破碎仪的原理并不是太神秘、太复杂。简单说就是将电能通过换能器转换为声能，这种能量通过液体介质(如水)而变成一个个密集的小气泡，这些小气泡迅速炸裂，产生的象小***一样的能量，从而起到破碎细胞等物质的作用(俗称“空化效应”)。
超声波细胞粉碎机是利用一种超声波在液体中产生空化效应的多功能、多用途的仪器；它能用于各种动植物细胞、病毒细胞、细菌及组织的破碎，也可用于各类无机物质的破碎重组，同时可用来乳化、分离、匀化、提取、消泡清洗及加速化学反应等。超声波细胞粉碎机有广泛的用途，如:
1、超声波提取生物纳米（超声波化学合成法）
超声波化学反应中，起关键作用的是声波的空化效应，在超声波的辐照过程中，在液体里将发生空化气泡的形成，长大和崩灭，当空化气泡崩灭时产生一个覆盖着的强压力脉冲，产生许多独特的性质，例如产生高达5000K的高温，大于200Mpa的压力，这就是超声波化学合成的能量来源，利用这些能量能在一些特殊粉末表面合成出纳米粒子。
2、超声波制药（1）注射用医药物质的分散——将磷脂类与胆固醇混合用适当方法与药物混合在水溶液中，经超声分散，可以得到更小粒子供静脉注射。
（2）草药提取——利用超声分散破坏植物组织，加速溶剂穿透组织作用，提高中草药有效成分提取率。如金鸡纳树皮中全部生物碱用一般方法侵出需5小时以上，采用超声分散只要半小时即可完成。
（3）制备混悬剂——在超声空化和强烈搅拌下，将一种固体药物分散在含有表面活性剂的水溶液中，可以形成1um左右口服或静脉注射混悬剂。例“静注喜树碱混悬剂”“肝脏造影剂”、“硫酸钡混悬剂”。
（4）制备疫苗——将细胞或病菌借助于超声分散将其杀死以后，再用适当方法制成疫苗。
3、超声波对化妆品的分散
为了更进一步提取药物精华和粒子微细化，并节约生产成本，达到分散、乳化效果，使化妆品更深入渗透到肌肤里层，让肌肤很好的吸收，发挥药物的效力和作用，采用超声波乳化可达到非常理想的效果。采用超声分散，则不需要使用乳化剂，就能使蜡及石蜡乳化、化妆水等油的微粒子分散。石腊在水中分散的粒子直径可达1um以下。
4、超声波对酒的醇化—催陈技术
一瓶美酒以它的酒味醇厚，绵软柔和、芳香浓郁为人青睐，人们常用陈年老酒来形容酒的珍贵，一瓶上世纪的陈酒，标价几万元，其价格的含义在于时间的存放上。酒的主要控制因素是化学变化即酸的形成，并进一步酯化，酯参与乙醇和水的缔合。刚出厂的酒含有戍醇，有辛辣味，这种气味要经过很长时间才能化解，这个缓慢变化称酒的醇化。用功率1.6KW，频率17.5-22KHZ的超声波处理5-10min，可使酒的老熟时间缩短1/3到1/2。
因此超声波细胞粉碎机可以并且已经被广泛用于生物化学、微生物学药理学、物理学、动物学、农学、医学、制药等领域的教学、科研、生产。
超声波细胞粉碎机运用窍门123
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天堂狗10462021-07-28
超声波细胞破碎仪就是将电能通过换能器转换为声能，这种能量通过液体介质而变成一个个密集的小气泡，这些小气泡迅速炸裂，产生的象小炸弹一样的能量，从而起到破碎细胞等物质的作用的仪器。
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咯叽咯叽子2017-11-01
如题，对这方面有了解的请告知一下，不要来打广告
比利时Bioruptor非接触式全自动超声破碎仪Bioruptor UCD 200,UCD ...123
suosuo3022021-07-30
目前实验室准备买这台仪器，因为常规超声做CHIP效果不好，有战友用过吗，效果怎样啊？？
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交联实验验证细胞中两个蛋白之间的相互作用，交联剂应用之后，电泳之前样本要怎样处理？是否需要超声破碎仪处理细胞？超声处理会不会破坏蛋白二聚体？
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●超声探头采用进口钛合金材质
●高能效换能器
●振幅自动调节，在不同的负载状况时振幅保持一致
●设置超声间歇时间
●微机控制,超声功率连续调节
●集成温度控制样品温度
●隔音箱均采用特殊隔音材料向左转|向右转
●高能效换能器
●振幅自动调节，在不同的负载状况时振幅保持一致
●设置超声间歇时间
●微机控制,超声功率连续调节
●集成温度控制样品温度
●隔音箱均采用特殊隔音材料向左转|向右转
宁波新芝超声波细胞破碎仪science iid功率怎么计算 分析行业新闻123
宰幼岚2021-08-22
在超声工作中用户只需要按(▲)或(▼)即可调节超声波的输出功率(按(▲)是增加功率，按(▼)是减少功率，本功能用与功率微调)，在调节时仪器的的输出功率是不停止的，在用户调节到需要的值后即可超声输出。
