
Product Highlights
- Reproducible – consistent results between technical replicates for increased confidence in results
- Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability
- Sensitive – reliable quantification of low abundance targets and scarce samples
- Robust – reliable, accurate detection of DNA and RNA targets from a broad range of sample types
- Fast – delivers reproducible, accurate assay results in as little as 30 minutes
Product Description
The SensiFAST™ SYBR No-ROX Kit has been developed for fast, highly accurate real-time PCR and has been validated on all commonly-used real-time instruments that do not require the passive reference dye ROX.
A combination of the latest advances in buffer chemistry and PCR enhancers ensures that the SensiFAST SYBR No-ROX Kit produces reliable assay results under fast thermal cycling conditions. An antibody-mediated hot-start DNA polymerase system promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range.
The SensiFAST SYBR®No-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.
Applications
- Gene expression analysis
- DNA / RNA target detection
- miRNA profiling / quantification
- Copy number variation (CNV) analysis

SensiFAST Products Customer Review
SensiFAST SYBR No-ROX One-Step Kit Customer Review
Real-Time PCR Selection Chart
One-step Vs. Two-step real-time RT PCR
A discussion of the pros and cons of each detection strategy.ebiomall.com






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可储存十套常规程序数据和一套组合程序,工作方式有定时和计数两种。
组合程序最多可以由十套常规程序组成,可选择循环或不循环工作模式。
超温保护及报警功能。
超声波输出强度自动限定功能。
定时方式工作时间定时:0~99小时59分59秒。
计数方式超声工作次数:0~9999次。
超声时间范围:0~99小时59分59秒。
间隙时间范围:0~99小时59分59秒。间隙时间=0为超声连续工作。
温度控制精度:±1℃。
工作电压:VAC100-240,50-60Hz。
工作环境:室内(无潮湿,无阳光直射,无腐蚀性气体向左转|向右转
●高能效换能器
●振幅自动调节,在不同的负载状况时振幅保持一致
●设置超声间歇时间
●微机控制,超声功率连续调节
●集成温度控制样品温度
●隔音箱均采用特殊隔音材料向左转|向右转
我们实验室比较简陋,没有超声破碎仪,能做原核表达吗?用溶菌酶代替可以吗

