DIY Human IFN Lambda 2/3 (IL-28A/B) ELISA (TCM)
- Specifications
- Tech Info / Data
- Citations
- Documentation
Specifications
Materials Provided:
- Capture Antibody
- Detection Antibody
- Standard
- Streptavidin-HRP
Tech Info / Data
This DIY ELISA is a Do-It-Yourself Development kit that contains the basic components necessary for the development of sandwich ELISAs to measure natural and recombinant Human Interleukin-28A (IFN-λ2), and natural and recombinant Human Interleukin-28B (IFN-λ3). DIY kits are intended for the analysis of cell culture supernates. Other matrices need to be evaluated on a case-by-case basis. When used according to instructions and recommended materials, each DIY kit contains sufficient materials to develop approximately fifteen 96-well ELISA plates.
IL-28A, IL-28B and IL-29 are a recently discovered class II cytokine family that displays similar properties to type I interferons (IFNs). Similarly, they have been shown to function by signaling through the JAK-STAT pathway, up-regulating the expression of genes involved in controlling viral replication and cellular proliferation. Consequently, they have also been described in the literature as lambda IFNs (IFN-λ1[IL- 29], IFN-λ2 [IL-28A] and IFN-λ3 [IL-28B]) or collectively as type III IFNs. Additional studies have shown that both type I and type III IFNs are upregulated during viral infection, suggesting each may have similar and perhaps distinct roles in controlling the host response to pathogens.
All type III IFNs signal through a heterodimer receptor complex comprised of the IL-10R2 and the IL-28αR receptor chains to initiate the signal transduction cascade. In contrast, all the type I IFNs (α, β, ε, κ, ω) initiate signaling by binding to the IFNAR1/ IFNAR2 receptor complex to promote signaling. IFNAR gene knockout studies have indicated that type III IFNs cannot effectively maintain a potent antiviral response. In contrast, IL- 28αR knockouts showed little effect on the overall antiviral response, suggesting that the role of type III interferons may be more selective to specific cells and viruses. However, either receptor knockout showed a strong reduction in reducing viral load when mice were treated with TLR3 and TLR9 agonists. Therefore, it may be possible that the type III IFNs have been evolutionarily conserved to combat pathogens that target specifically the IFNAR receptor complex or other unique aspects of the classical type I IFN pathways.
Citations
Documentation
61830 Protocol MSDSTypical Human IFN Lambda 2/3 (IL-28A/B) standard curve from 125 to 8000 pg/ml using PBL Human Interferon Lambda 2/3 ELISA for TCM (61830)
Assay Range: 125 - 8000 pg/ml
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谢谢！！！
我在论坛上搜索了一下，以前也有人问过类似的问题，但是都没有找到满意的答案。首先是人用的那种，用起来很不方便，并且反应偏慢，因为我做的是热效应，处理完后必须马上监测大鼠体温变化，等太久就不准了，因为一组动物往往有好几个。
以前用过一种进口的电子显示的，好像五六千块钱，但是现在怎么都查不到是在哪买的了。
哪位能提供帮助，感谢先！！
