Highlights
- Simple, rapid recovery of ultra-pure DNA from PCR, endonuclease digestions, and cell-free DNA preps, etc.
- Unique column construction allows sample loading and washing to be performed using a centrifuge, microcentrifuge, or vacuum source.
- Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
Description
| Detergent Tolerance | ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
|---|---|
| Elution Volume | ≥ 2 ml |
| Equipment | Microcentrifuge and centrifuge or vacuum source. |
| Purity | Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions. |
| Sample Source | DNA from large-scale sample sources including restriction endonuclease digestions and "crude" DNA preparations from cell-free lysates. |
| Size Range | 50 bp to 23 kb |
| Yield | ≤ 500 µg total DNA can be recovered.For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
Q1: What is the minimum input volume of DNA sample?
Working with volumes below 50 µl can result in decreased recovery. We recommend raising the starting volume to 100 µl with water to ensure optimal binding conditions.
Q2: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?
Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.
Q3: How many times can columns be reloaded?
We recommend no more than 5 times as binding efficiency might decrease.
Q4: How to process naked DNA stored in DNA/RNA Shield?
Use the standard protocol (add 2 or 5 volumes of DNA binding buffer depending on DNA size.
Q5: What is the lower limit and minimal amount of DNA that can be recovered?
Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.
Q6: What to do if ethanol addition to the DNA Wash Buffer was omitted?
The DNA will be eluted off the column. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.
“I sampled this kit next to a kit we already use. I had three of the same sample, two for the Zymo kit and one for the other. On one of the ones designated for your kit, I accidentally added the sample directly to the column filter, then added other reagents (I figured I might as well try it). The other I did as per the instructions. The one on which I goofed gave a similar purification and concentration as the other kit, while the one I did properly yielded approximately four times the DNA per µL as the other kit. I am very happy with this result, and when I rerun my samples, I plan to use your product.”
-Rosalind P. (University of Arkansas for Medical Sciences)
“Almost no loss of elution buffer at the final step. It is quite impressive compared to the other kits I have used, which lose about 5-7µl.”
-Joseph R. (Miller School of Medicine, University of Miami)
“It was a very simple procedure and it gave a concentration ten times the original amount.”
-Kimberly M. (University of Pennsylvania)
Read More| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| D3004-4-16 | DNA Elution Buffer | 16 ml | $18.00 | |
| D3004-4-50 | DNA Elution Buffer | 50 ml | $32.00 | |
| D4003-1-L | DNA Binding Buffer | 50 ml | $33.00 | |
| D4003-2-24 | DNA Wash Buffer (Concentrate) | 24 ml | $33.00 | |
| D4004-1-L | DNA Binding Buffer | 100 ml | $57.00 | |
| D4003-2-48 | DNA Wash Buffer (Concentrate) | 48 ml | $60.00 | |
| C1013-10 | Zymo-Spin VI Columns | 10 Pack | $44.00 | |
| C1013-20 | Zymo-Spin VI Columns | 20 Pack | $68.00 |
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超净台是正压装置,通过机械通风形成洁净操作面。只保护操作对象,不保护人,所以在操作可能的病原微生物及可能的危险对象时不可使用超净台。
2、接通电源,开启送风机。
3、工作台面上,不要存放不必要的物品,若放置纸张,请置于用自封袋内,以持工作区内的洁净气流不受干扰。4、操作结束后,清理工作台面,收集各废弃物,关闭风机及照明开关,用清洁剂擦拭,切断电源。5、要保持室内的干燥和清洁。潮湿的环境既会使材料锈蚀,影响电气电路的正常工作,另外潮湿空气还助长细菌、霉菌的滋生。6、定期对设备的清洁是正常使用的重要环节。清洁应包括使用前后的例行清洁和定期对过滤等主要部件的维护和更换处理。7、每天工作完毕后整理工作台,用蘸有擦拭液的纱布擦拭工作台,擦拭完毕后拉下玻璃门。设备内外表面应该光亮整洁,没有污迹,顶部没有灰尘。8、对工作区域进行打扫,以保持工作区域清洁无灰尘
9、每月进行一次维护检查,使工作台处于最佳状况,并填写维护记录。
10、高效过滤器的寿命2年,初效过滤器的寿命是6~8个月,按照使用寿命定期更换过滤器,并填写维护记录。
希望你采纳,谢谢!
测流式由侧面通过工作台(左右 上下皆可),于是缺点就是在气流层边缘可能出现负压,导致污染。而且其较为封闭,只有手伸入的圆洞,取拿物品较为
个人大胆一次,把内表面用酒精灯考一下,让表面重新融化。就可以了。各位可以用一块有机版的边缘试试。
不胜感激!!!!
祝大家研究顺利!!!

