Highlights
- Quick and easy 7 minute spin-column procedure to select for ≥ 300 bp, ≥ 200 bp, ≥ 150 bp, ≥ 100 bp, ≥ 50 bp DNA fragments or perform a double size selection.
- Clean and concentrate DNA from enzymatic reactions and library preparations in as little as 10 µl of nuclease free water.
- Eluted DNA is well suited for use in next generation sequencing, PCR, DNA ligation, endonuclease digestion, etc.
Description
| Applicable For | Next Generation sequencing, ligation reactions, PCR, labeling, and restriction endonuclease digestions. |
|---|---|
| Elution Volume | ≥ 10 µl of DNA Elution Buffer |
| Equipment | Microcentrifuge |
| Purity | A260/A280 > 1.8, A260/A230 > 1.8 |
| Sample Source | Purified or crude DNA samples. |
| Size Range | ~50 bp to 23 kb. 50 bp, 100 bp, 150 bp, 200 bp, 300 bp separation points. Double size selection: > 700 bp. |
| Yield | Up to 3 µg DNA |
Q1: Can the Select-a-Size kit bind genomic DNA?
Yes, it can be used to bind/purify/remove gDNA (all dsDNA >700 bp will be bound to the column).
Q2: Can the Select-a-Size DCC be used for clean-up of ssDNA?
No, it can be used for dsDNA only. For ssDNA selection we recommend agarose gel/PAGE purification.
Q3: What is the difference between Select-a-Size MagBeads and regular MagBinding Beads?
The two beads differ in terms of their coated functional groups which also lends itself to varying binding and elution efficiency.Select-a-Size MagBeadsMagBinding Beadsbind both DNA and RNA, at around >100 bp cutoff. The addition of isopropanol to the procedure allows to bind fragments less than 100 bp.–smaller diameter of beads–take longer to magnetize/pellet,–slightly shorter drying time–Used in Select-a-Size MagBead Kit and RCC MagBead Kit, Quick 16S Library Prep Kit–much larger diameter–faster to magnetize–longer to drying time–Used in all other MagBead kits
This kit worked incredibly well. I was pleased with the purity and concentration of the product.
-author -b
The kit worked great! Both my 260/230 and 260/280 ratios improved. I"m sold.
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Just like the other Zymo kits we used this worked perfectly! The protocol was simple to follow and the yield was amazing. I"ll definitely use this kit for my future cloning.
-Author
Read More| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| D3004-4-10 | DNA Elution Buffer | 10 ml | $14.00 | |
| D4080-1-15 | Select-A-Size Binding Buffer | 15 ml | $16.00 | |
| D4003-2-6 | DNA Wash Buffer (Concentrate) | 6 ml | $10.00 | |
| C1015-25 | Zymo-Spin IC-S Columns | 25 Pack | $34.00 | |
| C1001-50 | Collection Tubes | 50 Pack | $15.00 |
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超净工作台操作使用程序及规范(1)操作者需带好一次性口罩、帽子及医用乳胶手套。。(2)用双手轻轻抬起IVC笼盒外端,沿笼架搁挡向外移出笼盒,放在超净工作台旁的不锈钢小推车上。(3)用药液喷雾器充分喷洒双手手套外表及IVC笼盒的外面,消毒灭菌并沾粘住表面的尘埃,防止进入超净台时被层流气体吹扬。也可用带手套的双手浸入药液容器中,捞起并拧干药液毛巾,擦干手套外液滴,同时擦一下笼盒的外表。(4)打开超净工作台移门,把笼盒移入超净工作台。(5)适当拉下超净工作台移门。打开IVC笼盒盖底之间的紧固扣并打开笼盖。侧卧放在一边。更多超净工作台知识可进入上海巴玖查询!
2、关闭紫外灯,打开风机;
3、消毒液消毒台面、手
4、无菌操作
5、整理台面,关闭风机
超净台是正压装置,通过机械通风形成洁净操作面。只保护操作对象,不保护人,所以在操作可能的病原微生物及可能的危险对象时不可使用超净台。
下面是网上的操作规程
1.超净工作台操作使用前的准备工作
(1)开启超净工作台风机,净化台内环境。打开工作灯达到工作照度。
(2)检查超净工台中准备的各种灭菌饲养和实验物品是否齐全。若需更换笼盒。检查灭菌笼盒准备情况,并用不锈钢推车移至超净台移门边。
(3)检查喷雾器喷洒效果及超净台内外擦抹用的药液是否已配置好。
(4)打开房间内排风系统。排除打开笼盒时逸出的有味气体。
(5)检查IVC机组各种表头指针及目视各笼盒内的动物情况,估测IVC运行情况。
(6)记录影响实验动物繁殖、生长、发育的主要环境参数,如温度、相对湿度、日温差、笼盒内外压差等。
2.超净工作台操作使用程序及规范
(1)人员带好一次性口罩、帽子及医用乳胶手套。
(2)用双手轻轻抬起IVC笼盒外端,沿笼架搁挡向外移出笼盒,放在超净工作台旁的不锈钢小推车上。
(3)用药液喷雾器充分喷洒双手手套外表及IVC笼盒的外面,消毒灭菌并沾粘住表面的尘埃,防止进入超净台时被层流气体吹扬。也可用带手套的双手浸入药液容器中,捞起并拧干药液毛巾,擦干手套外液滴,同时擦一下笼盒的外表。
(4)打开超净工作台移门,把笼盒移入超净工作台。
(5)适当拉下超净工作台移门。打开IVC笼盒盖底之间的紧固扣并打开笼盖。侧卧放在一边。
谢谢采纳
【消过毒的无菌棉球】分几种
1、放在铁盒子里一起消过毒的
2、放在超净台上、用紫外照过30min的
急求,谢谢了!

