Human CD154 (CD40 Ligand) is a member of the tumor necrosis factor (TNF) family and is expressed on the surface of activated T cells. Interaction of CD154 and CD40 is essential for isotype switching in B cells. Known genetic defects that alter this interaction lead to impaired immune system function (1). CD154 has been shown to be hyperexpressed by B and T cells in SLE patients (4). CD154 has been reported to be expressed on vascular endothelial cells, smooth muscle cells and macrophages indicating a possible role for the CD40-CD154 immunoregulatory signaling mechanism during inflammation and immunity in atherogenesis (6).
Isotype: Murine IgG1
Immunogen: Human sgp39 fusion protein
Specificity: Antibody 24-31 immunoprecipitates a CD154 (gp39) molecule of about 39 kd. This clone has been reported to cross react with Cynomolgus and Rhesus Monkey CD154.
Functional Application: The antibody 24-31 will block MLR, sgp39 induced human B cell proliferation and T cell dependent B cell differentiation (9).
References:
1. D. Gray, et al, (1994) Seminars in Immunol 6: 303-310.
2. F. Pietravalle, et al, (1996) J Biol Chemistry 271: 5965-5967.
3. R.J. Noelle, (1996) Immunity 4: 415-419.
4. A. Desai-Mehta, et al, (1996) J Clin Invest 97: 2063-2073.
5. I.S. Grewal and R.A. Flavell, (1996) Immunol Today 17: 410-414.
6. F. Mach, et al, (1997) Proc Natl Acad Sci USA 94:1931-1936.
7. F. Pietraville, et al, (1996) Eur J. Immunol 26:725-728.
8. U.G. Wagner, C. Weyland, et al, (1998) J Immunol 161: 6390-6397.
9. M. Epeldegui, O. Martinez-Maza, et al. (2010) PLOS one 5(7): e11448. PMID: 20625427.
10. B Barnhart, L R Covey, et al. (2000) Immunology 99(1): 54-61. PMID: 10651941
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【消过毒的无菌棉球】分几种
1、放在铁盒子里一起消过毒的
2、放在超净台上、用紫外照过30min的
急求，谢谢了！
超净台是正压装置，通过机械通风形成洁净操作面。只保护操作对象，不保护人，所以在操作可能的病原微生物及可能的危险对象时不可使用超净台。
不胜感激！！！！
祝大家研究顺利！！！
超净工作台操作使用程序及规范(1)操作者需带好一次性口罩、帽子及医用乳胶手套。。(2)用双手轻轻抬起IVC笼盒外端，沿笼架搁挡向外移出笼盒，放在超净工作台旁的不锈钢小推车上。(3)用药液喷雾器充分喷洒双手手套外表及IVC笼盒的外面，消毒灭菌并沾粘住表面的尘埃，防止进入超净台时被层流气体吹扬。也可用带手套的双手浸入药液容器中，捞起并拧干药液毛巾，擦干手套外液滴，同时擦一下笼盒的外表。(4)打开超净工作台移门，把笼盒移入超净工作台。(5)适当拉下超净工作台移门。打开IVC笼盒盖底之间的紧固扣并打开笼盖。侧卧放在一边。更多超净工作台知识可进入上海巴玖查询！
2、关闭紫外灯，打开风机；
3、消毒液消毒台面、手
4、无菌操作
5、整理台面，关闭风机
