Molecular Structure: A soluble fusion protein consisting of the extracellular (137aa) domain of human CD279(PD-1): (25)ldspdrpwnpptfspallvvtegdnatftcsfsntsesfvlnwyrmspsnqtdklaafpedrsqpgqdcrfrvtqlpngrdfhmsvvrarrndsgtylcgaislapkaqikeslraelrvterraevptahpspspr(161)
Linking amino acids: sagtr
murine IgG2a Fc +hinge: (133 aa) eprgptikpcppckcpapnllggpsvfifppkikdvlmislspivtcvvvdvseddpdvqiswfvnnvevhtaqtqthredynstlrvvsalpiqhqdwmsgkefkckvnnkdlpapiertiskpkgsvrapqvyvlpppeeemtkkqvtltcmvtdfmpediyvewtnngktelnykntepvldsdgsyfmysklrvekknwvernsyscsvvheglhnhhttksfsrtpgk
In SDS-PAGE, this protein runs at 52 kd under native, and 27 kd under reducing conditions.
Data suggest that the murine Ig Fc proximal portion of this construct may be prone to cleavage.
Transfectant Cell Line: CHO
INFORMATION Human CD279 (PD-1, Programmed death 1) is a 55 kd Ig superfamily member with similarity to CD28 and CD152(CTLA-4). It is expressed on activated T and B and myeloid cells, and engagement by its ligands PD-L1 (CD274, B7-H1) or PD-L2 (B7-DC) can inhibit proliferation and cytokine expression. In mice blockade of PD-1 ligand interaction has been used to augment T cell anti cancer responses (1,2,3).
Recombinant CD279 -muIg binds to recombinant CD274 and CD273 in EIA and cell surface CD273 on moDC in FACS. It is recognized by anti-CD279 mAb (cat #279-030; clone ANC4H6) in EIA.
References: . 1) MA Curran, Allison JP, et al. (2010) PNAS 107(9): 4275-80. 2) Mangsbo SM, TH Totterman, et al. (2010) J Immunotherapy 33(3):225. 3) Hirano F, L Chen, et al. (2005) Canc Res 65: 1089.
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个人大胆一次，把内表面用酒精灯考一下，让表面重新融化。就可以了。各位可以用一块有机版的边缘试试。
可不可以在动物中心（SPF级别）取材后将组织再送到细胞室
而且，紫外在培养基中造成的富氧离子会杀伤细胞。
望采纳^O^
测流式由侧面通过工作台（左右 上下皆可），于是缺点就是在气流层边缘可能出现负压，导致污染。而且其较为封闭，只有手伸入的圆洞，取拿物品较为
