
Human CD137 (4-1BB) is expressed on activated T cells within 24-48 hours of activation (3). CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily (1,2). CD137 appears to be important for T cell proliferation and survival (4) and induces monocyte activation (5).
Molecular Structure: A soluble 55 kd fusion protein consisting of the extracellular (186aa) domain of human CD137 fused to human IgG1 Fc (4), with a predicted monomeric molecular weight of 44.1 kd.
Transfectant Cell Line: CHO
Functional Application: CD137 Ig fusion protein blocks binding of anti-human CD137 to activated CEM human tumor cells and also binds to CD137 Ligand on Raji cells.
References:
1. M.R. Alderson, et al, (1994) Eur J Immunol 24: 2219-2227.
2. H.J. Gruss, and S.K. Dower, (1995) Blood 85: 3378-3404.
3. B.A. Garni-Wagner, et al, (1996) Cellular Immunol 169: 91-98.
4. H. Schwarz, et al, (1996) Blood 87: 2839-2845.
5. J. Langstein, et al, (1998) J Immunol 160: 2488-2494.
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个人大胆一次,把内表面用酒精灯考一下,让表面重新融化。就可以了。各位可以用一块有机版的边缘试试。
可不可以在动物中心(SPF级别)取材后将组织再送到细胞室
而且,紫外在培养基中造成的富氧离子会杀伤细胞。
望采纳^O^
测流式由侧面通过工作台(左右 上下皆可),于是缺点就是在气流层边缘可能出现负压,导致污染。而且其较为封闭,只有手伸入的圆洞,取拿物品较为

