Human CD147 (EMMPRIN, Basigin) is a transmembrane glycoprotein expressed more intensely on thymocytes than on mature peripheral blood T cells. Expression of CD147 in bronchial epithelial cells has been correlated with incidence of COPD(3).
Isotype: Murine IgG1
Immunogen: Human T cell line Molt 13
Specificity: Antibody UM-8D6 recognizes extracellular epitope 2 within the n-terminal Ig domain (1) of the human CD147 molecule, about 35-40 kd (2)
Functional Applications: UM-8D6 inhibits homotypic aggregation, adhesion to matrix proteins and migation through matrigel (1). This antibody can inhibit homotypic induction of MMP (Matrix metalloproteinase), as well as cyclophilin mediated chemotactic resposes (4). It has been shown to inhibit proliferation of stimulated T cells in vitro (7). UM-8D6 is recommended for use in Western blot at 0.1 - 1 ug/ml (5). It works on acetone fixed frozen section IHC (6).
References:
1. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 760-766.
2. A.H. Kirsch, D.A. Fox et al, (1997) Tissue Antigens 50:147-152.
3. Jouneau S, K.F. Chung, et al. (May 2011) Respirology 16(14): 705-712.
4. Yurchenko V, M Bukrinsky, et al. (2010) Clin Exp Immunol 160(3): 305-317.
5. O Najyb, L Brissette, E Rassart (June 2015) J Biol Chem 290: 16077-16087. PMID: 25918162
6. Y Noguchi, A Ito, et al (2003) J CLin Endocrinol Metab 88(12): 6063-72. PMID: 14671212
7. Smriti M Agrawal, V Wee Yong, et al. (2012) J Neuroinflammation 9: 64. doi: 10.1186/1742-2094-9-64
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个人大胆一次，把内表面用酒精灯考一下，让表面重新融化。就可以了。各位可以用一块有机版的边缘试试。
可不可以在动物中心（SPF级别）取材后将组织再送到细胞室
而且，紫外在培养基中造成的富氧离子会杀伤细胞。
望采纳^O^
测流式由侧面通过工作台（左右 上下皆可），于是缺点就是在气流层边缘可能出现负压，导致污染。而且其较为封闭，只有手伸入的圆洞，取拿物品较为
