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Description
Details
This antibody recognizes the stage-specific embryonic antigen-3 (SSEA-3) that is expressed upon the surface of human teratocarcinoma stem (EC), embryonic germ (EG), embryonic stem (ES), and induced pluripotent stem (iPS) cells. No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-3 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES/iPS cells may be accompanied by an increase in SSEA-3 expression.
Sample Data

Figure A: FC analysis on H1 human ES cells at a 1:100 dilution.Red histogram represents SSEA-3 Antibody and open histogram represents isotype control. A PE-conjugated anti-Rat IgM was used as the secondary antibody. Figure B: ICC analysis on H1 human ES cells. Cells were stained with SSEA-3 Antibody at a 1:100 dilution followed by an Alexa 488-conjugated secondary antibody (green). Nuclei were counterstained with DAPI (blue).
Details
Details
| Concentration | 0.5 mg/mL |
|---|---|
| Species Reactivity | Human, Mouse |
| Host | Rat Monoclonal |
| Clone | MC-631 |
| Isotype | IgM |
| Immunogen | 4-8 cell stage mouse embryos |
| Formulation | Aqueous buffer, 0.09% sodium azide. |
| Storage and Stability | Store at 2-8°C. Stable for 6 months from date of receipt when stored as directed. |
| Applications Tested | Flow Cytometry (FC), Immunocytochemistry/Immunofluorescence (ICC/IF) |
| Recommended Dilutions | Flow Cytometry 1:100Immunocytochemistry/Immunofluorescence 1:100It is recommended that the antibody be titrated for optimal performance for each application. |
| Alternative Names | Stage-specific embryonic antigen 3 |
| References | Brimble, S.N., et al. (2007) The cell surface glycosphingolipids SSEA-3 and SSEA-4 are not essential for human ESC pluripotency. Stem Cells 25: 54-62. PMID: 17008424Henderson, J.K., et al. (2002) Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells 20: 329-337. PMID: 12110702Kannagi, R., et al. (1983) Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells. EMBO J 2: 2355-2361. PMID: 6141938 |
| Technical Documents | ST11014 Technical Data Sheet |
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怀着欣喜激动的心情做实验,可是。。。。。可是。。。。。超净台紫外灯忘记关了。。。。。痛心疾首,感觉不会再爱了,我连冻存都没冻过。。。。。
请各位大神指点焦虑的菜鸟,我的细胞系被照了四十多分钟,会有多大的影响,能不能继续用?对人的皮肤会不会有影响,现在过去了几个小时皮肤还挺正常的。
感谢!
2、接通电源,开启送风机。
3、工作台面上,不要存放不必要的物品,若放置纸张,请置于用自封袋内,以持工作区内的洁净气流不受干扰。4、操作结束后,清理工作台面,收集各废弃物,关闭风机及照明开关,用清洁剂擦拭,切断电源。5、要保持室内的干燥和清洁。潮湿的环境既会使材料锈蚀,影响电气电路的正常工作,另外潮湿空气还助长细菌、霉菌的滋生。6、定期对设备的清洁是正常使用的重要环节。清洁应包括使用前后的例行清洁和定期对过滤等主要部件的维护和更换处理。7、每天工作完毕后整理工作台,用蘸有擦拭液的纱布擦拭工作台,擦拭完毕后拉下玻璃门。设备内外表面应该光亮整洁,没有污迹,顶部没有灰尘。8、对工作区域进行打扫,以保持工作区域清洁无灰尘
9、每月进行一次维护检查,使工作台处于最佳状况,并填写维护记录。
10、高效过滤器的寿命2年,初效过滤器的寿命是6~8个月,按照使用寿命定期更换过滤器,并填写维护记录。
希望你采纳,谢谢!
个人大胆一次,把内表面用酒精灯考一下,让表面重新融化。就可以了。各位可以用一块有机版的边缘试试。
超净工作台只能保护在工作台内操作的试剂等不受污染,并不保护工作人员,而生物安全柜是负压系统,能有效保护工作人员。
测流式由侧面通过工作台(左右 上下皆可),于是缺点就是在气流层边缘可能出现负压,导致污染。而且其较为封闭,只有手伸入的圆洞,取拿物品较为

