Recombinant Human His6-HR6A/UBE2A Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E2-612
| Formulation | X mg/ml (XμM) in 50 mM HEPESpH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: HR6A/UBE2A
Homolog of Rad6 A (HR6A), also known as Ubiquitin-conjugating Enzyme E2A (UBE2A), is a ubiquitously expressed member of the Ubiquitin-conjugating (E2) enzyme family that is highly expressed in the brain and heart (1). HR6A/UBE2A has a predicted molecular weight of 17 kDa. The human protein shares 100% amino acid sequence identity with the mouse and rat orthologs.Like other E2 family members, HR6A/UBE2A has an E2 catalytic core domain that contains an active site cysteine residue required for the formation of a thioester bond with Ubiquitin (2). HR6A/UBE2A is involved in many cellular processes including protein degradation, transcriptional regulation, and the DNA damage response. HR6A/UBE2A functions with the UBR2 Ubiquitin ligase (E3) to mediate ubiquitination of target substrates degraded through the N-end rule proteolytic pathway (3). HR6A/UBE2A also interacts with the human BRE1 E3 to ubiquitinate Histone H2B on Lys120, which promotes transcription via methylation of Histone H3 on Lys4 (4). HR6A/UBE2A is activated via phosphorylation on Ser120 by CDK9 following exposure to UVC irradiation and HR6A/UBE2A-RAD18-dependent ubiquitination of PCNA is required for DNA damage tolerance (5-7). HR6A/UBE2A also regulates the mRNA and protein levels of p53 (8). Physiologically, this enzyme is required in female mice for the production of viable offspring (9). Mutations in human HR6A/UBE2A cause UBE2A deficiency syndrome in males, which is characterized by intellectual disability, seizures, absent speech, and urogenital and skin anomalies (10-12). This protein has an N-terminal His6-tag. (Accession NP_003327)
- Koken, M.H. et al. (1996) Dev. Biol. 173:119.
- Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
- Lee, P.C. et al. (2011) Mol. Cell 43:392.
- Kim, J. et al. (2009) Cell 137:459.
- Unk, I. et al. (2006) Proc. Natl. Acad. Sci. USA 103:18107.
- Yoon, J.H. et al. (2012) Proc. Natl. Acad. Sci. USA 109:7799.
- Shchebet, A. et al. (2012) Cell Cycle 11:2122.
- Chen, S. et al. (2012) Mol. Cell. Biol. 32:576.
- Roest, H.P. et al. (2004) Mol. Cell. Biol. 24:5485.
- Nascimento, R.M. et al. (2006) Am. J. Hum. Genet. 79:549.
- Honda, S. et al. (2010) J. Hum. Genet. 55:244.
- de Leeuw, N. et al. (2010) Am. J. Med. Genet. 152A:3084.
Citation for Recombinant Human His6-HR6A/UBE2A Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
1Citation: Showing 1 - 1
- Functional crosstalk between histone H2B ubiquitylation and H2A modifications and variantsAuthors: F Wojcik, GP Dann, LY Beh, GT Debelouchi, R Hofmann, TW MuirNat Commun, 2018;9(1):1394.Applications: Bioassay
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【消过毒的无菌棉球】分几种
1、放在铁盒子里一起消过毒的
2、放在超净台上、用紫外照过30min的
急求，谢谢了！
个人大胆一次，把内表面用酒精灯考一下，让表面重新融化。就可以了。各位可以用一块有机版的边缘试试。
测流式由侧面通过工作台（左右 上下皆可），于是缺点就是在气流层边缘可能出现负压，导致污染。而且其较为封闭，只有手伸入的圆洞，取拿物品较为
2、关闭紫外灯，打开风机；
3、消毒液消毒台面、手
4、无菌操作
5、整理台面，关闭风机
