easYmer HLA-A*30:02 MHC Tetramers Kit
easYmer HLA-A*30:02 MHC Tetramers Kit Developed and Manufactured by immunAware
Materials Included:
The easYmer kit contains a peptide receptive preparation of HLA, a folding buffer, a positive control peptide for HLA-complex folding. The exact identities of these components are given below:
- easYmer: HLA-A*30:02 – peptide receptive and biotinylated HLA-A*30:02 molecules in Tris/Maleate pH7 with 30% Glycerol
- Folding Buffer: Tris/Maleate pH7
- Peptide: YF NS5 286-295 (KSEYMTSWFY), a known HLA-A*30:02 binder. Positive control for evaluation analysis of peptide-HLA folding
for Research Use Only
Available Sizes:
- 20 Tests (Sample-size)
- 50 Tests (Standard Size)
- 150 Tests
- 500 Tests
Key Benefits of easYmer® MHC Tetramers
- Ready-to-use
- One step loading
- Completely flexible and customizable*
- Biotinylated
- No special equipment needed
- Long shelf-life
*For Custom Tetramer Production, please Contact Us for more information
Assay Principle
This protocol is designed to evaluate the efficiency of peptide-HLA-I interaction and complex formation. The assay is based on detecting the ß2-microglobulin (ß2m? light chain subunit of recombinant HLA Class I (HLA-I) comlexes, where the heavy chain has been biotin tagged. These tagged complexes are subsequently captured by streptavidin coated beads, labeled with PE-conjugated anti-human ß2m, and analyzed by flow cytometry. Since peptide-HLA-I complex formation is entirely peptide dependent, bead-associated signals will only be detected if the peptide in question supports the folding of the HLA-I allotype of interest; peptides that efficiently support folding will give strong signals whereas peptides that support folding sub-optimally, or not at all, will give moderate to non-detectable signals.
Products Related to easYmer HLA-A*30:02 MHC Tetramers
easYmer HLA-A*36:01 MHC Tetramers Kit
easYmer HLA-A*68:01 MHC Tetramers Kit
easYmer HLA-A*68:02 MHC Tetramers Kit
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
无管道型：内置高效活性碳过滤器，可直接排放到室内。
补风型：从室外补充空气，减轻实验室空调的负荷。
连体型：扩大实验空间，为大型仪器提供方便。
桌上型：装在实验台上的通风柜
两面型：从两面可以操作
落地型：适用于大型实验装置向左转|向右转
如何防止降解？
