Highlights
- Simple: Quickly and easily rescue plasmid from yeast.
- Efficient Isolation: Works well with low-copy and hard-to-isolate plasmids.
- High-Quality: Isolated plasmid DNA is ideal for molecular biology techniques, such as PCR, transformation, hybridization, etc.
Description
| Applicable For | Plasmid DNA is well suited for downstream applications such as PCR, transformation, hybridization and other sensitive applications |
|---|---|
| Elution Volume | ≥ 10 µl per well |
| Equipment | Centrifuge with microplate carriers |
| Processing Volume | ≤ 1.5 ml of Culture |
| Sample Source | Cell Culture (Colonies/Patches or Liquid Culture) |
| Size Range | Up to 25 kb. |
| Yield | Typically between 0.01-0.3 ng for most 2 µ based plasmids from 1.5 ml overnight cultures |
Q1: What is the difference between Zymoprep Yeast Plasmid Miniprep I and Miniprep II?
Both the Zymoprep Yeast Plasmid Miniprep I and II utilize the same chemistry for lysis; however, Miniprep I uses isopropanol precipitation and Miniprep II utilizes a column for purification. The Miniprep II allows for consistent yield and purity; and samples can be concentrated to a low elution volume.
Q2: What is the typical plasmid yield?
Typically, between 0.01 - 0.3 ng for most 2 µ based plasmid from 1.5 ml overnight cultures.In order to generate more plasmid, the plasmid is typically transformed into E. Coli, cultured, and isolated using a traditional E. Coli plasmid prep.
Q3: Can this kit be used to isolate linear plasmid DNA?
Yes
Q4: If I’m using stationary phase yeast cells, what can I do to improve sample lysis?
We generally recommend working with fresh or early log phase cells, which are easier to lyse. For stationary phase cells, user optimization is necessary, and we recommend increasing digestion to > 1 hour and/ or increasing the amount of Zymolyase.
Q5: What yeast strains are these kits compatible with?
Any strains susceptible to Zymolyase, which includes the following fungal genera: Asbya KloekeraCandidaKluyveromycesDebaryomycesLipomycesEremotheciumMetschikowiaEndomycesPichiaHansenulaPullulariaHanseniasporaSaccharomycesSaccaromycodesSaccharomycopsisSchizosaccahromycesTorulopsis
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| D2004-1-45 | Solution 1 Digestion Buffer | 45 ml | $88.00 | |
| D2004-1-90 | Solution 1 Digestion Buffer | 90 ml | $146.00 | |
| D2004-2-45 | Solution 2 Lysis Buffer | 45 ml | $88.00 | |
| D2004-2-90 | Solution 2 Lysis Buffer | 90 ml | $146.00 | |
| D2004-3-180 | Solution 3 Neutralizing Buffer | 180 ml | $146.00 | |
| D2004-3-90 | Solution 3 Neutralizing Buffer | 90 ml | $88.00 | |
| D4003-2-24 | DNA Wash Buffer (Concentrate) | 24 ml | $33.00 | |
| D4003-2-48 | DNA Wash Buffer (Concentrate) | 48 ml | $60.00 | |
| C1001-50 | Collection Tubes | 50 Pack | $15.00 | |
| C2002 | Collection Plate | 2 Plates | $22.00 | |
| C2003 | Elution Plate | 2 Plates | $19.00 | |
| C2004 | Zymo-Spin I-96 Plate | 2 Plates | $142.00 | |
| P1001-2 | 96-Well Block | 2 Blocks | $18.00 | |
| C2007-8 | 96-Well Plate Cover Foil | 8 Foils | $18.00 | |
| C2011-2 | Air Permeable Sealing Cover | 2 Pack | $12.00 | |
| C2011-4 | Air Permeable Sealing Cover | 4 Pack | $24.00 | |
| C2011-8 | Air Permeable Sealing Cover | 8 Pack | $42.00 |
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最好添上出处,谢谢各位!
1、在实验室内一切有可能产生毒性蒸气的工作必须在通风橱中进行,并有良好的排风设备。2、在稀释浓硫酸时,不能将水往浓硫酸里倒,而应将浓硫酸缓缓倒入水中,不断搅拌均匀。3、取用有毒、有恶臭味的试剂时,要在通风橱中操作;使用完毕后,将瓶口封严。4、化学危险物品应当分类、分项存放,还原性试剂与氧化剂、酸与碱类腐蚀剂等不得混放,相互之间保持安全距离。5、受阳光照射易燃烧、易爆炸或产生有毒气体的化学危险品应当在阴凉通风的地点存放。

