CD244 (2B4, SLAMF4) is a 66 kD member of the CD2/SLAM related receptor family (SRR). It is expressed on NK (3), Tcell subsets, monocytes and eosinophils(4). It is expressed at high levels on Leuikemia initiating cells, and plays a role in their proliferative potential (5). In mice there are two transcription variants, a long form and a short form of CD244 each of which have distinct signaling properties. In humans, only the long form is expressed.
Decreased CD244 expression on monocytes of SLE patients correlated (negatively) with autoantibody titer(2). CD244 mediated function in immune response can vary greatly dependant on the context of its ligation.
Molecular Structure: A soluble molecule consisting of murine CD8 alpha signal peptide residual amino acids and linker: (1)kpqapegkgc(10)
the mature extracellular domain of human CD244 (199aa): (11)qgsadhvvsisgvplqlqpnsiqtkvdsiawkkllpsqngfhhilkwengslpsntsndrfsfivknlsllikaaqqqdsglyclevtsisgkvqtatfqvfvfdkvekprlqgqgkildrgrcqvalsclvsrdgnvsyawyrgskliqtagnltyldeevdingthtytcnvsnpvsweshtlnltqdcqnahqefr(209) murine IgG2a Fc + hinge regions: (233aa) eprgptikpcppckcpapnllggpsvfifppkikdvlmislspivtcvvvdvseddpdvqiswfvnnvevhtaqtqthredynstlrvvsalpiqhqdwmsgkefkckvnnkdlpapiertiskpkgsvrapqvyvlpppeeemtkkqvtltcmvtdfmpediyvewtnngktelnykntepvldsdgsyfmysklrvekknwvernsyscsvvheglhnhhttksfsrtpgk (442aa total).
The molecule has a predicted monomeric non glycosylated molecular weight of 49.5 kd.. The molecule is dimeric. In SDS-PAGE, it runs at apporoximately 135 kD native, and 70 kD reduced.. CD244-muIg binds to recombinant CD48 in EIA and cell surface CD48 on Raji cell surface in FACS. This binding is blocked by anti-CD48 mAb (Cat#199-020).
Transfectant Cell Line: CHO
References:
1) Boles KS, Mathew PA, et al. (1999) Tissue Antigens 54(1): 27-34.
2) Mak A, AM Fairhurst, et al. (2017) Clinical Rheumatology 37(3): 811-816. doi.org/10.1007/s10067-017-3698-2.
3) Vacca P, MC Mingarri, et al. (2006) Blood 108: 4078-4085.
4) Munitz A, Levi-Schaffer F, et al. (2005) J Immunol 174(1): 110-118.
5) Zhang F, Zheng J, et al. (2017) Haematologica Doi: 10.3324/haematol.2016.151555.
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最好添上出处，谢谢各位！
1、在实验室内一切有可能产生毒性蒸气的工作必须在通风橱中进行，并有良好的排风设备。2、在稀释浓硫酸时，不能将水往浓硫酸里倒，而应将浓硫酸缓缓倒入水中，不断搅拌均匀。3、取用有毒、有恶臭味的试剂时，要在通风橱中操作；使用完毕后，将瓶口封严。4、化学危险物品应当分类、分项存放，还原性试剂与氧化剂、酸与碱类腐蚀剂等不得混放，相互之间保持安全距离。5、受阳光照射易燃烧、易爆炸或产生有毒气体的化学危险品应当在阴凉通风的地点存放。
