CD244 (2B4, SLAMF4) is a 66 kD member of the CD2/SLAM related receptor family (SRR). It is expressed on NK (3), Tcell subsets, monocytes and eosinophils(4). It is expressed at high levels on Leuikemia initiating cells, and plays a role in their proliferative potential (5). In mice there are two transcription variants, a long form and a short form of CD244 each of which have distinct signaling properties. In humans, only the long form is expressed.
Decreased CD244 expression on monocytes of SLE patients correlated (negatively) with autoantibody titer(2). CD244 mediated function in immune response can vary greatly dependant on the context of its ligation.
Molecular Structure: A soluble molecule consisting of murine CD8 alpha signal peptide residual amino acids and linker: (1)kpqapegkgc(10)
the mature extracellular domain of human CD244 (199aa): (11)qgsadhvvsisgvplqlqpnsiqtkvdsiawkkllpsqngfhhilkwengslpsntsndrfsfivknlsllikaaqqqdsglyclevtsisgkvqtatfqvfvfdkvekprlqgqgkildrgrcqvalsclvsrdgnvsyawyrgskliqtagnltyldeevdingthtytcnvsnpvsweshtlnltqdcqnahqefr(209) murine IgG2a Fc + hinge regions: (233aa) eprgptikpcppckcpapnllggpsvfifppkikdvlmislspivtcvvvdvseddpdvqiswfvnnvevhtaqtqthredynstlrvvsalpiqhqdwmsgkefkckvnnkdlpapiertiskpkgsvrapqvyvlpppeeemtkkqvtltcmvtdfmpediyvewtnngktelnykntepvldsdgsyfmysklrvekknwvernsyscsvvheglhnhhttksfsrtpgk (442aa total).
The molecule has a predicted monomeric non glycosylated molecular weight of 49.5 kd.. The molecule is dimeric. In SDS-PAGE, it runs at apporoximately 135 kD native, and 70 kD reduced.. CD244-muIg binds to recombinant CD48 in EIA and cell surface CD48 on Raji cell surface in FACS. This binding is blocked by anti-CD48 mAb (Cat#199-020).
Transfectant Cell Line: CHO
References:
1) Boles KS, Mathew PA, et al. (1999) Tissue Antigens 54(1): 27-34.
2) Mak A, AM Fairhurst, et al. (2017) Clinical Rheumatology 37(3): 811-816. doi.org/10.1007/s10067-017-3698-2.
3) Vacca P, MC Mingarri, et al. (2006) Blood 108: 4078-4085.
4) Munitz A, Levi-Schaffer F, et al. (2005) J Immunol 174(1): 110-118.
5) Zhang F, Zheng J, et al. (2017) Haematologica Doi: 10.3324/haematol.2016.151555.
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刚对实验室的通风柜做了个气流流向测试，报告出来了不知道该怎么管理这个文件了，当做设备确认方案？感觉不是很合理。放公司的安环部门？他们也没有档案室。我该怎么保存这个文件呢？
无管道型：内置高效活性碳过滤器，可直接排放到室内。
补风型：从室外补充空气，减轻实验室空调的负荷。
连体型：扩大实验空间，为大型仪器提供方便。
桌上型：装在实验台上的通风柜
两面型：从两面可以操作
落地型：适用于大型实验装置向左转|向右转
