Human CD70 (TNFSF7) is the ligand for CD27 (TNFRSF7) and is notably absent on resting lymphocytes. CD70 is expressed after activation on about 20% of T cells and on about 70% of B cells. Recombinant CD70-muCD8 binds to cell surface CD27 on human peripheral blood leukocytes. Recombinant CD70 constructs have been used to elicit T cell responses in vitro(6) and in vivo (5).
Molecular Structure: A soluble molecule consisting of the extracellular domain (167aa) of murine CD8 alpha fused to 146 (c-term) amino acids of the extracellular domain of human CD70, with a predicted monomeric weight of 34.8 kd.
Sequence for extracellular mature murine CD8alpha: (28) kpqapelrifpkkmdaelgqkvdlvcevlgsvsqgcswlfqnsssklpqptfvvymasshnkitwdeklnssklfsamrdtnnkyvltlnkfskenegyyfcsvisnsvmyfssvvpvlqkvnstttkpvlrtpspvhptgtsqpqrpedcrprgsvkgtgldfacd(194);
Linking amino acids: gt ;
extracellular human CD70: (48) lpleslgwdvaelqlnhtgpqqdprlywqggpalgrsflhgpeldkgqlrihrdgiymvhiqvtlaicssttasrhhpttlavgicspasrsisllrlsfhqgctivsqrltplargdtlctnltgtllpsrntdetffgvqwvrp(193).
Transfectant Cell Line: CHO
Functional Application: CD70-muCD8 binds to cell surface CD27 on a subset of T cells
References:
1) R.Q. Hintzen, et al, (1994) J Immunol 152: 1762-1773.
2) M.R. Bowman, et al, (1994) J Immunol 152: 1756-1761.
3) Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1137-1138.
4) K. Agematsu, et al, (1995) J Immunol 154: 3627-3635.
5) T. F. Rowley , A Al-Shamkhani, (2004) J Immunol 172: 6039-6046.
6) J. M.Carr, D. T. Fearon, et al. (2006) PNAS:103(51):19454-19459
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刚对实验室的通风柜做了个气流流向测试，报告出来了不知道该怎么管理这个文件了，当做设备确认方案？感觉不是很合理。放公司的安环部门？他们也没有档案室。我该怎么保存这个文件呢？
无管道型：内置高效活性碳过滤器，可直接排放到室内。
补风型：从室外补充空气，减轻实验室空调的负荷。
连体型：扩大实验空间，为大型仪器提供方便。
桌上型：装在实验台上的通风柜
两面型：从两面可以操作
落地型：适用于大型实验装置向左转|向右转
