
Human CD56 is an adhesion molecule from the Ig superfamily which is restricted to NK cells in the immune system. It is believed that NK cells form a first line of defense against tumor cells and cells infected with bacteria and viruses.
Molecular Structure: A soluble fusion protein consisting of the extracellular loop domains 1-4 (370aa) of human CD56 (20)LQVDIV….CTASN(389)
fused to murine IgG2a Fc and hinge (232aa), with a predicted monomeric molecular weight of kd 67.8.
Transfectant Cell Line: CHO
Functional Application: CD56(trn)-muIg fusion protein blocks binding of anti-human CD56 antibody (cat #308-020, but not cat #208-020) to NK cells present in ficoll prepared human PBL.
References:
S.P. Bourne, et al, (1991) J Neuro-Oncol 10: 111-119. T.L. Whiteside and R.B. Herberman (1994) Clinical & Diagnostic Laboratory Immunology. H. Spits, et al, (1995) Blood 85: 2654-2670.
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洗板机洗板是将手工操作改由洗板机进行,人为因素少,速度快,条件恒定,但是使用洗板机洗板的一个特点是,每次洗板后不能拍干,故有较多的液体残留,需增加洗板次数来减少这种误差。洗板机洗板次数越少,假阳性越高,本实验中,洗板1次,假阳性率高达90%;洗板2次,假阳性率为38%;洗板3次,假阳性率为2%;洗板4~5次,未出现假阳性。洗板次数并非越多越好,太多不但浪费人力、物力,并且也会导致假阴性结果,所以一般试剂盒要求洗板4~5次即可。洗板机洗板时应经常检查冲洗头是否通畅,若被杂物堵塞时可用注射器针头挑出。为了洗涤效果较好,实验室可采用机器与人工洗涤相结合的方法,在机器洗涤后再进行人工洗板1~2次,能达到理想的洗涤效果。
综上所述,在ELISA快速法检验中,手工法洗板和洗板机洗板没有本质的差别,只要操作合乎要求,均能得到正确、可靠的结果。展开

