DNA Clean & Concentrator-5
DNA Clean & Concentrator-5
| Cat # | Name | Size | Price | Quantity |
|---|
Documents
Highlights
- Clean and concentrate up to 5 µg DNA with ≥ 6 µl elution in as little as 2 minutes with 0 µl wash residue carryover.
- Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.
- Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
Description
| Detergent Tolerance | ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
|---|---|
| Elution Volume | ≥ 6 µl |
| Equipment | Microcentrifuge |
| Purity | A260/A280 > 1.8 |
| Sample Source | DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. |
| Size Range | 50 bp to 23 kb |
| Yield | ≤ 5 µg total DNA can be recovered.For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
Q1: What is the difference between capped and uncapped?
The only difference is the cap. Everything else between the columns (max capacity, column matrix, etc.) is the same. Unsure which to use? It mainly comes down to preference. Some people like the capped columns for easy labeling or added security while others like the uncapped columns for faster sample processing.
Q2: What is the minimum input volume of DNA sample?
Working with volumes below 50 µl can result in decreased recovery. Zymo Research recommend raising the starting volume to 100 µl with water to ensure optimal binding conditions.
Q3: How many times can columns be reloaded?
Zymo Research recommends reloading columns no more than 5 times, as binding efficiency might decrease.
Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?
Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.
Q5: How can I process naked DNA stored in DNA/RNA Shield?
Add an equal volume of ethanol (95-100%) to the sample and mix well. The sample is ready-to-bind and does not require DNA Binding Buffer. Proceed to Step 2.
Q6: What is the lower limit and minimal amount of DNA that can be recovered?
Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.
Q7: What should I do if I did not add ethanol to the DNA Wash Buffer before starting?
The DNA will be eluted off the column during the wash step. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.
“The elution volume is low enough to concentrate any sample very well and the efficiency is not compromised at all.”
-Shanshan L. (UT Southwestern)
"I had low-volume, low-concentration, high-molecular weight genomic DNAs for high-throughput sequencing that I could not get to be perfectly clean without losing a significant amount of my (precious) sample. The Qiagen products were recommended by the company doing the sequencing, and they just ate up my sample. When I received this sample with my mini-prep kit, I was at the end of the line and figured I"d give it a try, despite being warned against anything involving spin columns that may shear the DNA. It worked beautifully. We can proceed with our project. No loss of sample, the gel looks the same (no sign of DNA shearing), and what an easy and fast protocol! Thank you, Zymo!"
-Cindy T.(University of Iowa)
“I sampled this kit next to a kit we already use. I had three of the same sample, two for the Zymo kit and one for the other. On one of the ones designated for your kit, I accidentally added the sample directly to the column filter, then added other reagents (I figured I might as well try it). The other I did as per the instructions. The one on which I goofed gave a similar purification and concentration as the other kit, while the one I did properly yielded approximately four times the DNA per µL as the other kit. I am very happy with this result, and when I rerun my samples, I plan to use your product.”
-Rosalind P. (University of Arkansas for Medical Sciences)
Read More| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| D3004-4-1 | DNA Elution Buffer | 1 ml | $11.00 | |
| D3004-4-4 | DNA Elution Buffer | 4 ml | $10.00 | |
| D4003-1-25 | DNA Binding Buffer | 25 ml | $21.00 | |
| D4003-1-L | DNA Binding Buffer | 50 ml | $33.00 | |
| D4003-2-24 | DNA Wash Buffer (Concentrate) | 24 ml | $33.00 | |
| D4003-2-6 | DNA Wash Buffer (Concentrate) | 6 ml | $10.00 | |
| C1001-50 | Collection Tubes | 50 Pack | $15.00 | |
| C1001-500 | Collection Tubes | 500 Pack | $52.00 | |
| C1001-1000 | Collection Tubes | 1000 Pack | $90.00 | |
| C1003-50 | Zymo-Spin I Columns | 50 Pack | $50.00 | |
| C1003-250 | Zymo-Spin I Columns | 250 Pack | $214.00 | |
| C1004-50 | Zymo-Spin IC Columns | 50 Pack | $53.00 | |
| C1004-250 | Zymo-Spin IC Columns | 250 Pack | $228.00 | |
| D4004-1-L | DNA Binding Buffer | 100 ml | $57.00 |
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上海浸泰专注碳氢清洗机16年！
铭扬超声波清洗机小编为您解答：超声波清洗机的power99%是你机台额定功率的百分比，意思就是机台额定功率是1000W，power99%显示现在机台工作功率达到了990W
2、超声波清洗槽尺寸选择：根据工件大小和清洗量决定。
3、超声波清洗剂的选择：以传统方法用到的清洗剂是一样的，不需要更换。
4、超声波频率的选择：常规的是28KHZ和40KHZ,28KHZ的特点是超声威力大，超声次数少，适合清洗清洁度有一定要求的工件。40KHZ的特点是超声威力小，超声次数多，适合清洗清洁度要求不高的工件。
5、超声波功率的选择：根据清洗槽的尺寸来确定功率大小。每个50W的换能器之间的距离为80mm，根据这个行业标准，可以算出功率大小。
6、配件选择：韩国逸山超声波换能器、第六代超声波发生器、SUS304槽体、进口胶水和种钉双重固定。
7、超声波清洗液温度的选择：常温-100度，具备恒温系统，一直保持这样的温度。比如：可以在60度的时候，去油效果更佳。
8、超声波安装方式的选择：超声波安装方式有：底部安装、两侧安装、底部和两侧三面同时安装。底部安装又叫做底震式，超声波的有效作用高度为500mm，两侧安装是侧震，超声波的有效作用长度为1000mm，有效作用高度与换能器的位置有关。底部和两侧三面同时安装，是三面震，超声波的有效作用长度不能超过1000mm，高度可以调整。
雷士超声波清洗机主要应用于医院手术刀、镊子、止血钳、内镜活检钳、注射用针头、各式大小注射器、试管、玻璃瓶、玻璃片、换药碗、各种盘子、圆桶等放射性、污染性器具的大批量高洁度的清洗和消毒，是医院手术室、化验室、检验室、供应室、牙科、消毒中心以及科研单位，制药厂的必备设备。
1. 清洗的工件：应该清楚的了解需要清洗的产品材质构成、形状结构、尺寸大小、清洗量、清洗洁净度要求、清洗完成后工序是什么等。
2. 需清洗的污物：明确工件表面需要清洗的污质是什么，比如：冲压拉升油脂、抛光腊、积炭、灰尘、指纹等。
3. 清洗工艺：根据工件的特质及污质情况选择合理的超声波清洗设备工艺，您可以咨询专家为您提供这方面的建议，或者请专业厂家为您提供专业的工艺实验来完善清洗工艺，清洗工艺的选择是设备制造前的重要部分，一定要选择正确的工艺，否则影响将来长期的清洗效果。
4. 如何选择手动、半自动和全自动：手动一般是适合于简单工序、小量清洗的操作。半自动适合清洗大型的重物或者是中大批量的清洗，可以减轻劳动力，提高清洗效率。全自动适合大批量的清洗，清洗质量稳定，效果好，但是设备造价成本高。还有一个是用户需要根据自身的预算情况等多方面的因素来综合考虑。
5.怎样选择清洗槽的大小：清洗槽的大小选择是根据工件清洗量，结构，清洗时间来确定，也就是在一定的清洗时间需要完成这么多的清洗工件。清洗一次的时间得出需要一次清洗多少产品在槽内所占的位置大小来确定清洗槽的大小。
6.如何选择超声波合理的功率强弱：功率的强弱大小对工件的清洗效果好环及清洗时间是一个直接决定因素。功率强弱大小是根扰工件结构及工件在槽内摆放方式来选择超声波的辐射分布方式等。来计算发射面所需要超声波功率的大小。佳姆信的超声波功率大小计算采用国际通的标准方式来计算。
7.怎样选择超声波的频率：一般常规的清洗频率选择为28KHZ和40KHZ比较多，对工件表面要求极微小的颗粒及表面不允许损伤的才选用较高的频率。
8.如何选择超声波清洗设备清洗剂：清洗剂为以后设备长期使用的消耗品，清洗剂分为有机溶剂类和水基类的清洗剂。根据配方的不同有上千上万种，每一种清洗剂根据清洗工件的材质，特性，污物情况等来选择清洗剂，一般原则是既能快速彻底的将污物清洗干净，但又不能破坏工件外观性为基本。
1）超声波不是靠电机产生的，而是靠超声波震头。
2）超声波按功能分种类很多，电机可能是实现其它功能的。
3）如果超声波的产生是靠电机的，我也见过，那是换概念，
那不叫超声波，原因，超声波的率频是28KHZ，如果用电机加
扁心轮的话，一秒钟，28000转，一分钟，168万转，没有这样的电机，
所以用电机带动的，叫声波，不叫超声波。
