DNA Clean & Concentrator-5
DNA Clean & Concentrator-5
| Cat # | Name | Size | Price | Quantity |
|---|
Documents
Highlights
- Clean and concentrate up to 5 µg DNA with ≥ 6 µl elution in as little as 2 minutes with 0 µl wash residue carryover.
- Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.
- Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
Description
| Detergent Tolerance | ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
|---|---|
| Elution Volume | ≥ 6 µl |
| Equipment | Microcentrifuge |
| Purity | A260/A280 > 1.8 |
| Sample Source | DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. |
| Size Range | 50 bp to 23 kb |
| Yield | ≤ 5 µg total DNA can be recovered.For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
Q1: What is the difference between capped and uncapped?
The only difference is the cap. Everything else between the columns (max capacity, column matrix, etc.) is the same. Unsure which to use? It mainly comes down to preference. Some people like the capped columns for easy labeling or added security while others like the uncapped columns for faster sample processing.
Q2: What is the minimum input volume of DNA sample?
Working with volumes below 50 µl can result in decreased recovery. Zymo Research recommend raising the starting volume to 100 µl with water to ensure optimal binding conditions.
Q3: How many times can columns be reloaded?
Zymo Research recommends reloading columns no more than 5 times, as binding efficiency might decrease.
Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?
Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.
Q5: How can I process naked DNA stored in DNA/RNA Shield?
Add an equal volume of ethanol (95-100%) to the sample and mix well. The sample is ready-to-bind and does not require DNA Binding Buffer. Proceed to Step 2.
Q6: What is the lower limit and minimal amount of DNA that can be recovered?
Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.
Q7: What should I do if I did not add ethanol to the DNA Wash Buffer before starting?
The DNA will be eluted off the column during the wash step. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.
“The elution volume is low enough to concentrate any sample very well and the efficiency is not compromised at all.”
-Shanshan L. (UT Southwestern)
"I had low-volume, low-concentration, high-molecular weight genomic DNAs for high-throughput sequencing that I could not get to be perfectly clean without losing a significant amount of my (precious) sample. The Qiagen products were recommended by the company doing the sequencing, and they just ate up my sample. When I received this sample with my mini-prep kit, I was at the end of the line and figured I"d give it a try, despite being warned against anything involving spin columns that may shear the DNA. It worked beautifully. We can proceed with our project. No loss of sample, the gel looks the same (no sign of DNA shearing), and what an easy and fast protocol! Thank you, Zymo!"
-Cindy T.(University of Iowa)
“I sampled this kit next to a kit we already use. I had three of the same sample, two for the Zymo kit and one for the other. On one of the ones designated for your kit, I accidentally added the sample directly to the column filter, then added other reagents (I figured I might as well try it). The other I did as per the instructions. The one on which I goofed gave a similar purification and concentration as the other kit, while the one I did properly yielded approximately four times the DNA per µL as the other kit. I am very happy with this result, and when I rerun my samples, I plan to use your product.”
-Rosalind P. (University of Arkansas for Medical Sciences)
Read More| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| D3004-4-1 | DNA Elution Buffer | 1 ml | $11.00 | |
| D3004-4-4 | DNA Elution Buffer | 4 ml | $10.00 | |
| D4003-1-25 | DNA Binding Buffer | 25 ml | $21.00 | |
| D4003-1-L | DNA Binding Buffer | 50 ml | $33.00 | |
| D4003-2-24 | DNA Wash Buffer (Concentrate) | 24 ml | $33.00 | |
| D4003-2-6 | DNA Wash Buffer (Concentrate) | 6 ml | $10.00 | |
| C1001-50 | Collection Tubes | 50 Pack | $15.00 | |
| C1001-500 | Collection Tubes | 500 Pack | $52.00 | |
| C1001-1000 | Collection Tubes | 1000 Pack | $90.00 | |
| C1003-50 | Zymo-Spin I Columns | 50 Pack | $50.00 | |
| C1003-250 | Zymo-Spin I Columns | 250 Pack | $214.00 | |
| C1004-50 | Zymo-Spin IC Columns | 50 Pack | $53.00 | |
| C1004-250 | Zymo-Spin IC Columns | 250 Pack | $228.00 | |
| D4004-1-L | DNA Binding Buffer | 100 ml | $57.00 |
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1、电路短路：
线路接错，或者是线路老化等，都有可能导致电路发生短路，从而导致超声波清洗机发生跳闸。
解决措施：纠正错误接线，或者是更换老化的线路。
2、整流桥短路
超声波发生器的整流桥发生短路，也会导致超声波清洗机跳闸。
解决措施：
用万用表的二极管档，逐个测量其整流桥的管教，如果发现有短路的管脚，则证明整流桥已经损毁，需要更换新的整流桥。
3、清洗频率设定不合适：
超声波清洗机里面的物品过多，且设定的清洗频率很高的情况下，易产生跳闸现象。
解决措施：
降低清洗频率。
二、关于超声波清洗机：
超声波在液体中传播，使液体与清洗槽在超声波频率下一起振动，液体与清洗槽振动时有自己固有频率，这种振动频率是声波频率，所以人们就听到嗡嗡声。随着清洗行业的不断发展，越来越多的行业和企业运用到了超声波清洗机。
对超声波清洗机原理由超声波发生器发出的高频振荡信号，通过换能器转换成高频机械振荡而传播到介质－－清洗溶剂中，超声波在清洗液中疏密相间的向前辐射，使液体流动而产生数以万计的直径为50-500μm 的微小气泡，存在于液体中的微小气泡在声场的作用下振动。这些气泡在超声波纵向传播的负压区形成、生长，而在正压区，当声压达到一定值时，气泡迅速增大，然后突然闭合。并在气泡闭合时产生冲击波，在其周围产生上千个大气压，破坏不溶性污物而使他们分散于清洗液中，当团体粒子被油污裹着而黏附在清洗件表面时，油被乳化，固体粒子及脱离，从而达到清洗件净化的目的。在这种被称之为“空化”效应的过程中，气泡闭合可形成几百度的高温和超过1000个气压的瞬间高压。
超声波清洗机的优点是：超声波清洗效果好，操作简单。人们所听到的声音是频率20-20000Hz的声波信号，高于20000Hz的声波称之为超声波，声波的传递依照正弦曲线纵向传播，产生大量小气泡 。一个原因是液体内局部出现拉应力而形成负压，压强的降低使原来溶于液体的气体过饱和，而从液体逸出，成为小气泡；另一原因是强大的拉应力把液体“撕开”成一空洞，称为空化。
但当超声波功率弱的时候，就不是清洗时间长与短的问题了，
而在28KHz到128KHz之间主要用在工序间洗净，40KHz以下的换能器主要用于五金类精密金属类，它强度大，但粗糙，一般精密光学零件都是采用超高频换能器，目前国内的洗净设备还没有达到兆赫兹的制造水平！日本的可以
楼主的问题还不好回答，洗净时间的长短与被洗物及洗净液有关，而并非是越长越好！
只是120KHz的超声机比28KHz的超声机价格高三倍，更高频率的恐怕更是吓人哟。有5分钟的 ，也有半小时的。也和你医疗器械的难易有关，比如含油含血的还是只表面的灰尘的清洗呢！
1、使用前请仔细阅读使用说明书。
2、使用清洗机前，必须有可靠的接地装置。
3、工作现场严禁携带火种及明火作业。(使用酒精、丙酮、汽油、等挥发易燃液体时，应加盖，此时严禁使用加热功能。)
4、清洗液温度最好不高于60℃，否则会影响清洗效果，一般为常温和状态下即可。
5、槽内无清洗液，不得开机，以免清洗机空载损坏。
6、清洗机长期不用时，应将槽内清洗液放掉，并将机体擦洗干净。
7、清洗机应放置在干燥、清洁的房间使用，严禁在潮湿、污染、阳光直射的地方使用。
上海浸泰专注碳氢清洗机16年！
2、超声波清洗槽尺寸选择：根据工件大小和清洗量决定。
3、超声波清洗剂的选择：以传统方法用到的清洗剂是一样的，不需要更换。
4、超声波频率的选择：常规的是28KHZ和40KHZ,28KHZ的特点是超声威力大，超声次数少，适合清洗清洁度有一定要求的工件。40KHZ的特点是超声威力小，超声次数多，适合清洗清洁度要求不高的工件。
5、超声波功率的选择：根据清洗槽的尺寸来确定功率大小。每个50W的换能器之间的距离为80mm，根据这个行业标准，可以算出功率大小。
6、配件选择：韩国逸山超声波换能器、第六代超声波发生器、SUS304槽体、进口胶水和种钉双重固定。
7、超声波清洗液温度的选择：常温-100度，具备恒温系统，一直保持这样的温度。比如：可以在60度的时候，去油效果更佳。
8、超声波安装方式的选择：超声波安装方式有：底部安装、两侧安装、底部和两侧三面同时安装。底部安装又叫做底震式，超声波的有效作用高度为500mm，两侧安装是侧震，超声波的有效作用长度为1000mm，有效作用高度与换能器的位置有关。底部和两侧三面同时安装，是三面震，超声波的有效作用长度不能超过1000mm，高度可以调整。
2、超声波清洗工件时，所标的功率大多是声频功率而不是电功率。一般超声波清洗机所标，是指消耗工频功率，而超声波清洗质量优劣是由效率来判断。如果效率低，在同样清洗效果时则会耗电大，这样反而增加了用户的费用。
总之，超声波清洗机不是功率越大清洗工件就会越干净，买超声波清洗机时因根据工件要求选择合适的功率。力华机械是专业生产超声波清洗机厂家，可根据您的需求非标订制各种超声波清洗设备。
铭扬超声波清洗机小编为您解答：超声波清洗机的power99%是你机台额定功率的百分比，意思就是机台额定功率是1000W，power99%显示现在机台工作功率达到了990W
还有水箱温度的控制
1、看频率：F≥20KHz（在实际应用中因为效果相似，通常把F≥15K的声波也称为超声波）。
2、看功率密度：p=发射功率(W)/发射面积(cm2);通常p≥0.3w/cm2;在液体中传播的超声波能对物体表面的污物进行清洗，其原理可用“空化”现象。
