Human CD272 (BTLA, B and T Lymphocyte Attenuator) is a member of the Immunoglobulin superfamily and has homology to CD152(CTLA-4). Engagement of BTLA by its co receptor CD270 (HVEM, a TNF superfamily member) can down regulate activated T and B cell responses. BTLA levels on antigen specific CD8+ T cells have been reported to decrease in viral specific, but not melanoma specific activated lines (1).
Recombinant CD272-muIg binds to recombinant HVEM-muIg in EIA.
Ancell has a matched pair of antibodies (cat # 372-020 and 272-030) for capture and detection of CD272 protein in EIA (see attachment).
Molecular Structure: A soluble fusion protein consisting of the muCD8 apha signal peptide residual amino acids+ linker: (1) kpqapelrgs(10)
CD272 mature EC: (11) ipyldiwnihgkescdvqlyikrqsehsilagdpfelecpvkycanrphvtwcklngttcvkledrqtswkeeknisffilhfepvlpndngsyrcsanfqsnli eshsttlyvtdvksaserpskdemasrp(143)
Linker +Murine IgG2a Hinge + Fc : (144) gteprgptikpcppckcpapnllggpsvfifppkikdvlmislspivtcvvvdvseddpdvqiswfvnnvevhtaqtqthredynstlrvvsalpiqhqdwmsgk efkckvnnkdlpapiertiskpkgsvrapqvyvlpppeeemtkkqvtltcmvtdfmpediyvewtnngktelnykntepvldsdgsyfmysklrvekknwverns yscsvvheglhnhhttksfsrtpg(378)
Predicted monomeric (non glycosylated) molecular weight: 43.0 kd. The molecule is dimeric and runs at ~100 kd in SDS-PAGE under native conditions, and ~50kd reduced.
Transfectant Cell Line:CHO
References:
1) Derre’ L, DE Speiser, et al. (2010) J Clin Invest 120(1):157-167.
2) Pasero C,D Olive, et al. (2009) Curr Mol Med 9 (7): 911-927.
3) Gavrieli M, KM Murphy, et al. (2006) Adv Immunol 92: 152-185.
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2、超声波清洗槽尺寸选择：根据工件大小和清洗量决定。
3、超声波清洗剂的选择：以传统方法用到的清洗剂是一样的，不需要更换。
4、超声波频率的选择：常规的是28KHZ和40KHZ,28KHZ的特点是超声威力大，超声次数少，适合清洗清洁度有一定要求的工件。40KHZ的特点是超声威力小，超声次数多，适合清洗清洁度要求不高的工件。
5、超声波功率的选择：根据清洗槽的尺寸来确定功率大小。每个50W的换能器之间的距离为80mm，根据这个行业标准，可以算出功率大小。
6、配件选择：韩国逸山超声波换能器、第六代超声波发生器、SUS304槽体、进口胶水和种钉双重固定。
7、超声波清洗液温度的选择：常温-100度，具备恒温系统，一直保持这样的温度。比如：可以在60度的时候，去油效果更佳。
8、超声波安装方式的选择：超声波安装方式有：底部安装、两侧安装、底部和两侧三面同时安装。底部安装又叫做底震式，超声波的有效作用高度为500mm，两侧安装是侧震，超声波的有效作用长度为1000mm，有效作用高度与换能器的位置有关。底部和两侧三面同时安装，是三面震，超声波的有效作用长度不能超过1000mm，高度可以调整。
