INFORMATION: Human CD123 (Interleukin 3 Receptor alpha) is a 70kD type I transmembrane molecule, and is the low affinity receptor for the cytokine IL-3, which can stimulate proliferation or differentiation. When paired in a heterodimer with CD131 (IL-3 R beta), it binds IL-3 with much higher affinity. CD123 is found on Myeloid precusors, Stem cells, a subset of T cells, some B cells Megakaryocytes, basophils, monocytes and epithelial cells. CD123 is present at high levels on many hematologic malignancies and antibodies(2) and CAR T cells(1) against CD123 have been used successfully to combat Acute Myeloid Leukemia.
Molecular Structure: A soluble molecule consisting of the extracellular domain of mature human CD123 fused to murine IgG2a Fc.
Mature CD123(EC) (307 aa): tkedpnppitnlrmkakaqqltwdlnrnvtdiecvkdadysmpavnnsycqfgaislcevtnytvrvanppfstwilfpensgkpwagaenltcwihdvdflscswavgpgapadvqydlylnvanrrqqyeclhyktdaqgtrigcrfddisrlssgsqsshilvrgrsaafgipctdkfvvfsqieiltppnmtakcnkthsfmhwkmrshfnrkfryelqiqkrmqpviteqvrdrtsfqllnpgtytvqirarervyeflsawstpqrfecdqeegantrawrtsllialgtllalvcvfvic
Linking amino acids (2aa): gt
Murine IgG2aFc (233aa): eprgptikpcppckcpapnllggpsvfifppkikdvlmislspivtcvvvdvseddpdvqiswfvnnvevhtaqtqthredynstlrvvsalpiqhqdwmsgkefkckvnnkdlpapiertiskpkgsvrapqvyvlpppeeemtkkqvtltcmvtdfmpediyvewtnngktelnykntepvldsdgsyfmysklrvekknwvernsyscsvvheglhnhhttksfsrtpgk
Predicted nonglycosylated monomeric weight: 61.6 kd.
Transfectant Cell Line: CHO
References:
1) Pizzitola I, Bonnet D, et al. (2014) Leukemia 28(8): 1596-1605. doi: 10.1038/leu.2014.62
2) Jin L, Lock RB, et al. (2009) Cell Stem Cell 5(1): 31-42. doi: 10.1016/j.stem.2009.04.018.
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2、超声波清洗槽尺寸选择：根据工件大小和清洗量决定。
3、超声波清洗剂的选择：以传统方法用到的清洗剂是一样的，不需要更换。
4、超声波频率的选择：常规的是28KHZ和40KHZ,28KHZ的特点是超声威力大，超声次数少，适合清洗清洁度有一定要求的工件。40KHZ的特点是超声威力小，超声次数多，适合清洗清洁度要求不高的工件。
5、超声波功率的选择：根据清洗槽的尺寸来确定功率大小。每个50W的换能器之间的距离为80mm，根据这个行业标准，可以算出功率大小。
6、配件选择：韩国逸山超声波换能器、第六代超声波发生器、SUS304槽体、进口胶水和种钉双重固定。
7、超声波清洗液温度的选择：常温-100度，具备恒温系统，一直保持这样的温度。比如：可以在60度的时候，去油效果更佳。
8、超声波安装方式的选择：超声波安装方式有：底部安装、两侧安装、底部和两侧三面同时安装。底部安装又叫做底震式，超声波的有效作用高度为500mm，两侧安装是侧震，超声波的有效作用长度为1000mm，有效作用高度与换能器的位置有关。底部和两侧三面同时安装，是三面震，超声波的有效作用长度不能超过1000mm，高度可以调整。
