The human B cell activating factor (BAFF) and APRIL(a proliferation inducing ligand) are both type II molecules belonging to the TNF superfamily. They are expressed by non-B cells, and are down-regulated by mitogenic stimulation.2 BAFF and APRIL bind to at least two receptors: TACI (transmembrane activator and CAML-interactor) and BCMA (B cell maturation antigen), both of which are restricted to B cells.3,4 Ligation of these receptors with recombinant BAFF dramatically increases IgM production by peripheral blood B cells1. Recently a third receptor for BAFF (BAFF-R) was described.5Cell surface BAFF can be cleaved to form a soluble trimeric molecule.2 BCMA-muIg binds to recombinant BAFF-muCD8 and can inhibit binding of this molecule to receptors on Raji cells.
Molecular Structure: A soluble molecule consisting of the murine CD8 alpha signal peptide( 10 amino acids), extracellular (51aa) domain of human BCMA fused to the murine IgG2a Fc (235 aa).
Predicted non glycosylated monomeric weight: 33 kd.
Sequence:
Residual signal peptide and linker amino acids (1) kpqapelrgs(10)
BCMA mature extracellular domain: (11)agqcsqneyfdsllhacipcqlrcssntppltcqrycnasvtnsvkgtnai(61)
linker + Murine IgG2a Fc + Hinge domain:
(62) gteprgptikpcppckcpapnllggpsvfifppkikdvlmislspivtcvvvdvseddpdvqiswfvnnvevhtaqtqthredynstlrvvsalpiqhqdwmsgkefkckvnnkdlpapiertiskpkgsvrapqvyvlpppeeemtkkqvtltcmvtdfmpediyvewtnngktelnykntepvldsdgsyfmysklrvekknwvernsyscsvvheglhnhhttksfsrtpg(296)
Transfectant Cell Line: CHO
Functional Application: BCMA-muIg binds to recombinant BAFF-muCD8 and can inhibit this molecule’s abiltiy to bind to receptors on Raji cells. It has been used in vitro to block binding of recombinant BAFF (6) and function of APRIL (7).
References:
1) Schneider P., J. Tschopp, et al. J. Exp. Med. 1999, 189 (11):1747-1756.
2) Shu, H.B., H. Johnson, W.H. Hui. J Leukoc Biol 1999, 65:680-683.
3) Marsters, S.A., A. Ashkenazi, et al. 2000, Curr Biol 10:785-788.
4) Xia, X., H. Hsu, et al. 2000, J Exp Med , 192(1): 137-143.
5) Thompson J.S., C. Ambrose, et al. Science 2001, 293: 2108-2111.
6) Xu W, A Cerutti, et al. (2007) Nat Immunol 8(3): 294-303. PMID: 17259987.
7) Bing He, Andrea Cerutti, et al. (2007) Immunity 26(6): 812-826.https://doi.org/10.1016/j.immuni.2007.04.014
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2、超声波清洗槽尺寸选择：根据工件大小和清洗量决定。
3、超声波清洗剂的选择：以传统方法用到的清洗剂是一样的，不需要更换。
4、超声波频率的选择：常规的是28KHZ和40KHZ,28KHZ的特点是超声威力大，超声次数少，适合清洗清洁度有一定要求的工件。40KHZ的特点是超声威力小，超声次数多，适合清洗清洁度要求不高的工件。
5、超声波功率的选择：根据清洗槽的尺寸来确定功率大小。每个50W的换能器之间的距离为80mm，根据这个行业标准，可以算出功率大小。
6、配件选择：韩国逸山超声波换能器、第六代超声波发生器、SUS304槽体、进口胶水和种钉双重固定。
7、超声波清洗液温度的选择：常温-100度，具备恒温系统，一直保持这样的温度。比如：可以在60度的时候，去油效果更佳。
8、超声波安装方式的选择：超声波安装方式有：底部安装、两侧安装、底部和两侧三面同时安装。底部安装又叫做底震式，超声波的有效作用高度为500mm，两侧安装是侧震，超声波的有效作用长度为1000mm，有效作用高度与换能器的位置有关。底部和两侧三面同时安装，是三面震，超声波的有效作用长度不能超过1000mm，高度可以调整。
