Recombinant Human His6-UBE2N/UBE2V2 Complex Protein, CF Summary
Recombinant Human His6-UBE2N/UBE2V2 Complex is a member of the Ubiquitin conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial His6-UBE2N/UBE2V2 Complex concentration of 0.1-1 μM.
18 kDa (UBE2N), 17 kDa (UBE2V2)
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E2-666
| Formulation | 0.88 mg/ml (25 μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 2 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: UBE2N/UBE2V2 Complex
Ubiquitin conjugating Enzyme E2N (UBE2N), also known as Ubiquitin conjugating Enzyme 13 (Ubc13), forms a functional complex with the catalytically inactive UBE2V2 (human homologue of yeast MMS2) protein (1). Human UBE2N/Ubc13 shares 100% and 99% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively, while human UBE2V2 shares 99% aa sequence identity with its mouse and rat orthologs. The UBE2N/UBE2V2 Complex functions with Ubiquitin ligases (E3s), including RNF111 and RNF8, to synthesize Lys63-linked Ubiquitin chains (2,3) that can either be unanchored or attached to target proteins (4, 5). The UBE2N/UBE2V2 complex has important roles in facilitating responses to various forms of DNA damage (2, 6).
- Hofmann R.M.& Pickart C.M. (1999) Cell 96: 645
- Poulsen, S.L et al. (2013) Journ Cell Biol. 201: 797
- Campbell, S.J. et al. (2012) Journ. Biol. Chem. 287: 23900
- Xia, Z.P. et al. (2009) Nature 461:114.
- Liu, C. et al. (2009) Sci. Signal. 2:ra63.
- Wen, R. et al. (2012) DNA Repair 11: 157
Citation for Recombinant Human His6-UBE2N/UBE2V2 Complex Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
1Citation: Showing 1 - 1
- PI5P4K? functions in DTX1-mediated Notch signalingAuthors: L Zheng, SD ConnerProc. Natl. Acad. Sci. U.S.A., 2018;0(0):.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human His6-UBE2N/UBE2V2 Complex Protein, CF
There are currently no reviews for this product. Be the first toreview Recombinant Human His6-UBE2N/UBE2V2 Complex Protein, CF and earn rewards!
Have you used Recombinant Human His6-UBE2N/UBE2V2 Complex Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
雷士超声波清洗机主要应用于医院手术刀、镊子、止血钳、内镜活检钳、注射用针头、各式大小注射器、试管、玻璃瓶、玻璃片、换药碗、各种盘子、圆桶等放射性、污染性器具的大批量高洁度的清洗和消毒，是医院手术室、化验室、检验室、供应室、牙科、消毒中心以及科研单位，制药厂的必备设备。
Ultrasonic cleaning machine using process
超声波清洗机使用流程
Ultrasonic cleaning machine using process
使超声波清洗效果最佳的方式
(1)超声波清洗机槽的温度最好为30~50℃。
(2)根据不同的清洗对象正确选择清洗剂。清洗剂一般分为水基(碱性)清洗剂、有机溶剂清洗剂和化学反应清洗剂。通常使用最多的为水基清洗剂。
(3)根据被清洗物的污染程度和污垢性质，选用不同的清洗时间。超声波清洗机的清洗液可以循环使用，这种清洗方式可以节约大量的清洗液，每次使用完清洗机后，最好将清洗液放入容器中，下次使用时再倒入清洗槽，余下的沉淀物可处理掉。如清洗液的浓度不够时，适量加入一点即可重复使用。
超声波清洗机使用的是一种非常严密的清洗工艺，因此，超声波清洗机和清洗液的选择也是非常关键的，这直接影响着清洗的效果如何。所以，我们在购买清洗设备之前，就需要对被清洗部件做分析。
首先，我们需要明确被清洗对象的材料构成、结构以及数量，进而来分析并明确要清除的污染物，这些因素都是决定所要使用什么样的清洗液。通过分析我们就可以判断出是用溶剂清洗液还是用水性清洗液。
其次，最终的清洗工艺选择还需做清洗实验来证实哪一种才是最为合适的。只有这样，才能提供合适的超声波清洗机、设计合理的清洗工序以及清洗液来进行清洗作业 。
最后需要考虑清洗液的物理特性对超声波清洗机的影响，其中表面张力、蒸汽压、密度以及黏度是最明显的影响因素。除此之外，温度也是影响因素之一，通常也会影响空化作用的效率。
总而言之，要想使超声波清洗机达到理想的清洗效果和目标，在清洗之前作必要的分析是非常必要的，是必不可少的重要环节。
上海浸泰专注碳氢清洗机16年！
铭扬超声波清洗机小编为您解答：超声波清洗机的power99%是你机台额定功率的百分比，意思就是机台额定功率是1000W，power99%显示现在机台工作功率达到了990W
