| Trioxsalenintercalates into DNA and forms DNA single-strand adducts and interstrand crosslinks when activated with ultraviolet light |
Sample solution is provided at 25 µL, 10mM.
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- Purity = 98.00%
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Chemical structure
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| Cas No. | 3902-71-4 | SDF | Download SDF |
| Synonyms | NSC 71047,4,5’,8-Trimethylpsoralen,Trisoralen | ||
| Chemical Name | 2,5,9-trimethyl-7H-furo[3,2-g][1]benzopyran-7-one | ||
| Canonical SMILES | CC1=CC2=CC(C(C)=C3)=C(C(C)=C2O1)OC3=O | ||
| Formula | C14H12O3 | M.Wt | 228.2 |
| Solubility | Soluble in dichloromethane | Storage | Store at -20°C |
| Physical Appearance | A crystalline solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
Trioxsalen has been reported to intercalate into DNA forming DNA single-strand adducts and interstrand crosslinks when activated with UV light.
In vitro: Trioxsalen (trimethylpsoralen, trioxysalen or trisoralen) is a furanocoumarin and a psoralen derivative. It is obtained from several plants, mainly Psoralea corylifolia. Like other psoralens it causes photosensitization of the skin. After photoactivation it creates interstrand cross-links in DNA, which can cause programmed cell death unless repaired by cellular mechanisms. In research it can be conjugated to dyes for confocal microscopy and used to visualize sites of DNA damage [1].
In vivo: Mice received 3H-trioxsalen either orally or intraperitoneally. It was found that over 88% of trioxsalen, after po or i.p. administration, was excreted in the urine within 8 hours and over 90% within 12 hours. The distribution patterns of trioxsalen radioactivity demonstrated that trioxsalen was selectively present in liver, skin, and blood and was barely detectable in other organs. The highest values were observed between 2 and 6 hours and diminished rapidly thereafter [1].
Clinical trial: In men receiving 40 mg unlabelled trioxsalen, 80% of the administered dose was excreted in urine within 8 hours as hydroxylated or glucuronide derivatives. It was also reported that trioxsalen transformed to a principal metabolite 5"-carboxy-4,8-dimethylpsoralen, which was found to be inactive as skin photosensitizer and to this transformation had been attributed the difference in the photoreactivity of trioxsalen when topically applied or systematically administered [1].
Reference:[1] Mahmoud M.A. Hassan. Trioxsalen. Analytical Profiles of Drug Substances Volume 10, 1981, Pages 705-727.
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1.传质。就是底物或代谢产物更好在体系内转移和发挥作用。
2.溶氧。在好氧培养过程中，空气是滤过开放的，所以通过摇到可以让更多空气中氧气溶解于发酵液中。
3.体系均一。有便于对不同参数的取样测定。
2.溶氧，在好氧培养过程中，空气是滤过开放的，所以通过摇到可以让更多空气中氧气溶解于发酵液中。厌氧则不是这个作用了。 3.体系均一，有便于对不同参数的取样测定。。
光生化研究用的就有：脱色摇床，反应摇床，培养摇床；
培养摇床又分水浴摇床和气浴摇床（又称恒温振荡培养箱）。可见恒温培养箱与摇床不是一个概念，只是两者有交集————恒温振荡培养箱既属摇床的一个分支，又属恒温培养箱的一个分支。
如题，WB中，脱脂牛奶封闭时，忘记使用脱色摇床摇晃，对封闭效果影响大不大？有没有什么补救办法？延长封闭时间还是怎么办？急等，谢谢！！！！
