| CarboplatinAntitumor agent that forms platinum-DNA adducts. |
Sample solution is provided at 25 µL, 10mM.
Nature.2017 Jan 19;541(7637):417-420.
Nature.2018 Nov;563(7731):407-411.Nature.2018 Jun 13.Nature.2018 Jun 27.Nature.2018 Mar 29;555(7698):673-677.Nature.2017 Sep 7;549(7670):96-100.Nature.2016 Apr 21;532(7599):398-401.
Science.2016 Aug 5;353(6299)594-8Nat Nanotechnol.2017 Dec;12(12):1190-1198.Nature Biotechnology.2017 Jun;35(6):569-576Nat Med.2018 Sep 17.Cell.2018 Dec 21. pii: S0092-8674(18)31561-7.Cell.Available online 25 October 2018.Cell.2018 Sep 27. pii: S0092-8674(18)31183-8.Cell.2018 Jun 28;174(1):172-186.e21.Cell.2018 Feb 22;172(5):1007-1021.e17.Cell.2017 Nov 30;171(6):1284-1300.e21.Cell.2017 Aug 17. pii: S0092-8674(17)30869-3.Cell.2017 Jul 13;170(2):312-323Nat Med.2018 Jan 29.Nat Med.2017 Nov;23(11):1342-1351.Cell.2017 Apr 6;169(2):286-300.Cell.2015 Aug 27;162(5):987-1002.Cell.2015 Feb 12;160(4):729-44.Nature Medicine.2017 Apr;23(4):493-500.Cancer Cell.2018 May 14;33(5):905-921.e5.Cancer Cell.2018 Apr 9;33(4):752-769.e8.Cancer Cell.2018 Mar 12;33(3):401-416.e8.Cancer Cell.2017 Aug 14;32(2):253-267.e5.Nat Methods.2018 Jul;15(7):523-526.Cell Stem Cell.2018 May 3;22(5):769-778.e4.Cell Stem Cell.2017 Nov 20. pii: S1934-5909(17)30375-2.Quality Control & MSDS
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- Purity = 98.74%
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Chemical structure
| Cell experiment [1]: | |
Cell lines | A2780, SKOV3, IGROV-1 and HX62 cells |
Preparation method | The solubility of this compound in DMSO is limited. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months. |
Reacting condition | 0 ~ 200 μM; 72 hrs |
Applications | In a panel of human ovarian cancer cell lines, e.g. A2780, SKOV3 and IGROV-1 cells, Carboplatin significantly inhibited cell proliferation, with the IC50 values of 6.1 μM, 12.4 μM and 2.2 μM, respectively. When it was combined with 17-allylamino-17-demethoxygeldanamycin (17-AAG), antagonism instead of synergistic effect was indicated. |
| Animal experiment [1]: | |
Animal models | Nude mice bearing A2780 tumors |
Dosage form | 60 mg/kg; i.p. |
Applications | In nude mice bearing A2780 tumors, Carboplatin alone exhibited a modest antitumor effect, with the relative tumor volume of 8.4 on day 6. Moreover, the tumor weight relative to control (T/C) was 67%. When Carboplatin was combined 17-AAG, significantly greater antitumor activity was shown with the relative tumor volume and the T/C value reduced to 4.2 and 22% on day 6, respectively. |
Other notes | Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
References: [1]. Banerji U, Sain N, Sharp SY, et al. An in vitro and in vivo study of the combination of the heat shock protein inhibitor 17-allylamino-17-demethoxygeldanamycin and carboplatin in human ovarian cancer models. Cancer Chemother Pharmacol, 2008, 62(5): 769-778. | |
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| Cas No. | 41575-94-4 | SDF | Download SDF |
| Chemical Name | azane;cyclobutane-1,1-dicarboxylate;platinum(2+) | ||
| Canonical SMILES | C1CC(C1)(C(=O)[O-])C(=O)[O-].N.N.[Pt+2] | ||
| Formula | C6H12N2O4Pt | M.Wt | 371.25 |
| Solubility | ≥42.2 mg/mL in DMSO with ultrasonic and warming, ≥9.28 mg/mL in H2O with gentle warming, <2.18 mg/ml="" in="" etoh=""> | Storage | Store at -20°C |
| Physical Appearance | A solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
Carboplatin is a DNA synthesis inhibitor.
DNA synthesis is the natural or artificial creation of deoxyribonucleic acid (DNA) molecules.
Carboplatin inhibits DNA synthesis by binding to DNA and interfering with repair mechanism. In MTT assays with A2780, SKOV-3, IGROV-1 and HX62 human ovarian cancer cells, Carboplatin inhibited cell proliferation with IC50 of 6.2 μM, 12.4 μM, 2.2 μM and 116 μM for A2780, SKOV-3, IGROV-1 and HX62, respectively [1]. In UMC-11, H727 and H835 lung carcinoid cell line, Carboplatin also shows the anti-proliferative activities [2].
In xenograft-bearing mice, Carboplatin (60 mg/kg) had a modest antitumor effect and the relative tumor volumes on day 6 were 8.4 relative to the control of 11.9 [1]. Treatment of 56 small cell lung carcinoma patients with Carboplatin (300-400 mg/m2), 23 patients achieved a response including 5 complete remissions. 18 of 30 previously untreated patients achieved a response. Carboplatin was well tolerated and there was no nephrotoxicity [3].
References:[1]. Banerji U, Sain N, Sharp SY, et al. An in vitro and in vivo study of the combination of the heat shock protein inhibitor 17-allylamino-17-demethoxygeldanamycin and carboplatin in human ovarian cancer models. Cancer Chemother Pharmacol, 2008, 62(5): 769-778. [2]. Fiebiger W, Olszewski U, Ulsperger E, et al. In vitro cytotoxicity of novel platinum-based drugs and dichloroacetate against lung carcinoid cell lines. Clin Transl Oncol, 2011, 13(1): 43-49. [3]. Smith IE, Evans BD. Carboplatin (JM8) as a single agent and in combination in the treatment of small cell lung cancer. Cancer Treat Rev, 1985, 12 Suppl A: 73-75.
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培养皿上的细菌是一个月前接种的，培养基成分没有变化
如题，WB中，脱脂牛奶封闭时，忘记使用脱色摇床摇晃，对封闭效果影响大不大？有没有什么补救办法？延长封闭时间还是怎么办？急等，谢谢！！！！
1.传质。就是底物或代谢产物更好在体系内转移和发挥作用。
2.溶氧。在好氧培养过程中，空气是滤过开放的，所以通过摇到可以让更多空气中氧气溶解于发酵液中。
3.体系均一。有便于对不同参数的取样测定。
