| CarboplatinAntitumor agent that forms platinum-DNA adducts. |
Sample solution is provided at 25 µL, 10mM.
Nature.2017 Jan 19;541(7637):417-420.
Nature.2018 Nov;563(7731):407-411.Nature.2018 Jun 13.Nature.2018 Jun 27.Nature.2018 Mar 29;555(7698):673-677.Nature.2017 Sep 7;549(7670):96-100.Nature.2016 Apr 21;532(7599):398-401.
Science.2016 Aug 5;353(6299)594-8Nat Nanotechnol.2017 Dec;12(12):1190-1198.Nature Biotechnology.2017 Jun;35(6):569-576Nat Med.2018 Sep 17.Cell.2018 Dec 21. pii: S0092-8674(18)31561-7.Cell.Available online 25 October 2018.Cell.2018 Sep 27. pii: S0092-8674(18)31183-8.Cell.2018 Jun 28;174(1):172-186.e21.Cell.2018 Feb 22;172(5):1007-1021.e17.Cell.2017 Nov 30;171(6):1284-1300.e21.Cell.2017 Aug 17. pii: S0092-8674(17)30869-3.Cell.2017 Jul 13;170(2):312-323Nat Med.2018 Jan 29.Nat Med.2017 Nov;23(11):1342-1351.Cell.2017 Apr 6;169(2):286-300.Cell.2015 Aug 27;162(5):987-1002.Cell.2015 Feb 12;160(4):729-44.Nature Medicine.2017 Apr;23(4):493-500.Cancer Cell.2018 May 14;33(5):905-921.e5.Cancer Cell.2018 Apr 9;33(4):752-769.e8.Cancer Cell.2018 Mar 12;33(3):401-416.e8.Cancer Cell.2017 Aug 14;32(2):253-267.e5.Nat Methods.2018 Jul;15(7):523-526.Cell Stem Cell.2018 May 3;22(5):769-778.e4.Cell Stem Cell.2017 Nov 20. pii: S1934-5909(17)30375-2.Quality Control & MSDS
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- Purity = 98.74%
- COA (Certificate Of Analysis)
- HPLC
- NMR (Nuclear Magnetic Resonance)
- MSDS (Material Safety Data Sheet)
- Datasheet
Chemical structure
| Cell experiment [1]: | |
Cell lines | A2780, SKOV3, IGROV-1 and HX62 cells |
Preparation method | The solubility of this compound in DMSO is limited. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months. |
Reacting condition | 0 ~ 200 μM; 72 hrs |
Applications | In a panel of human ovarian cancer cell lines, e.g. A2780, SKOV3 and IGROV-1 cells, Carboplatin significantly inhibited cell proliferation, with the IC50 values of 6.1 μM, 12.4 μM and 2.2 μM, respectively. When it was combined with 17-allylamino-17-demethoxygeldanamycin (17-AAG), antagonism instead of synergistic effect was indicated. |
| Animal experiment [1]: | |
Animal models | Nude mice bearing A2780 tumors |
Dosage form | 60 mg/kg; i.p. |
Applications | In nude mice bearing A2780 tumors, Carboplatin alone exhibited a modest antitumor effect, with the relative tumor volume of 8.4 on day 6. Moreover, the tumor weight relative to control (T/C) was 67%. When Carboplatin was combined 17-AAG, significantly greater antitumor activity was shown with the relative tumor volume and the T/C value reduced to 4.2 and 22% on day 6, respectively. |
Other notes | Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
References: [1]. Banerji U, Sain N, Sharp SY, et al. An in vitro and in vivo study of the combination of the heat shock protein inhibitor 17-allylamino-17-demethoxygeldanamycin and carboplatin in human ovarian cancer models. Cancer Chemother Pharmacol, 2008, 62(5): 769-778. | |
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| Cas No. | 41575-94-4 | SDF | Download SDF |
| Chemical Name | azane;cyclobutane-1,1-dicarboxylate;platinum(2+) | ||
| Canonical SMILES | C1CC(C1)(C(=O)[O-])C(=O)[O-].N.N.[Pt+2] | ||
| Formula | C6H12N2O4Pt | M.Wt | 371.25 |
| Solubility | ≥42.2 mg/mL in DMSO with ultrasonic and warming, ≥9.28 mg/mL in H2O with gentle warming, <2.18 mg/ml="" in="" etoh=""> | Storage | Store at -20°C |
| Physical Appearance | A solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
Carboplatin is a DNA synthesis inhibitor.
DNA synthesis is the natural or artificial creation of deoxyribonucleic acid (DNA) molecules.
Carboplatin inhibits DNA synthesis by binding to DNA and interfering with repair mechanism. In MTT assays with A2780, SKOV-3, IGROV-1 and HX62 human ovarian cancer cells, Carboplatin inhibited cell proliferation with IC50 of 6.2 μM, 12.4 μM, 2.2 μM and 116 μM for A2780, SKOV-3, IGROV-1 and HX62, respectively [1]. In UMC-11, H727 and H835 lung carcinoid cell line, Carboplatin also shows the anti-proliferative activities [2].
In xenograft-bearing mice, Carboplatin (60 mg/kg) had a modest antitumor effect and the relative tumor volumes on day 6 were 8.4 relative to the control of 11.9 [1]. Treatment of 56 small cell lung carcinoma patients with Carboplatin (300-400 mg/m2), 23 patients achieved a response including 5 complete remissions. 18 of 30 previously untreated patients achieved a response. Carboplatin was well tolerated and there was no nephrotoxicity [3].
References:[1]. Banerji U, Sain N, Sharp SY, et al. An in vitro and in vivo study of the combination of the heat shock protein inhibitor 17-allylamino-17-demethoxygeldanamycin and carboplatin in human ovarian cancer models. Cancer Chemother Pharmacol, 2008, 62(5): 769-778. [2]. Fiebiger W, Olszewski U, Ulsperger E, et al. In vitro cytotoxicity of novel platinum-based drugs and dichloroacetate against lung carcinoid cell lines. Clin Transl Oncol, 2011, 13(1): 43-49. [3]. Smith IE, Evans BD. Carboplatin (JM8) as a single agent and in combination in the treatment of small cell lung cancer. Cancer Treat Rev, 1985, 12 Suppl A: 73-75.
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光生化研究用的就有：脱色摇床，反应摇床，培养摇床；
培养摇床又分水浴摇床和气浴摇床（又称恒温振荡培养箱）。可见恒温培养箱与摇床不是一个概念，只是两者有交集————恒温振荡培养箱既属摇床的一个分支，又属恒温培养箱的一个分支。
ZD-85双功能气浴恒温摇床型号：
（1）CHA－S型、恒温气浴摇床：属于－往复式
（2）SHZ-82型、恒温气浴摇床：属于－回旋式
（3）ZD－85型、恒温气浴摇床：属于－往复和回旋式、双功能。
ZD-85双功能气浴恒温摇床主要技术参数：
1、 使用电源： 220V 50Hz
2、 加热功率： 400w
3、 定时范围： 0~120分（或常开）
4、 振荡频率： 起动—300转/分,可调
5、 振荡幅度： 20mm
6、 恒温范围： 室温—50℃
7、 振荡方法： 往复、回旋和双功能（采购时选择）
8： 温控精度： +0.5℃
9： 装瓶量：试管：16×300mm 100ml×24只、200ml×15只
10： 外形尺寸： 700×470×500mm
THZ-82水浴恒温摇床技术指标
温控均匀性：≤±0.5℃
温控范围：室温-100 ℃
温度波动：±0.5℃
加热功率：1800W
振荡频率：起动-280次/分
振幅：20mm
定时范围：0-120分(或常开)
工作电源：AC220V 50Hz
水浴恒温摇床型号与振荡方式
SHA-C型为往复水浴恒温摇床
THZ-82型为回旋水浴恒温摇床
SHA-B型为双功能水浴恒温摇床
大容量恒温摇床参数 产品名称
双层恒温摇床
产品型号TS-1112B
显示方式液晶一屏显示
电源电压AC220V 50HZ
控温范围室温+5～60℃
温度分辨率0.1℃
照明方式内置照明灯
制冷方式自动
压缩机化霜方式自动
波动度±0.1℃
旋转频率30-280rpm
转速精度±1rpm
摆振幅度0-50mm无极可调
标准配置250ml*45支 500ml*36支
最大容量250ml*90支或50ml*72支或1000ml*36支
托盘尺寸970*560mm
震荡方式回旋式
工作环境温度5℃～40℃
输入功率750W
内胆尺寸（W*D*H）mm1120*640*830mm（595L）
外形尺寸（W*D*H）mm1200*800*150mm
拖盘数量2块
净重360KG
定时范围0～999小时
参考资料:Hi.baidu/=。www.jingda17.net。DI展开
2.溶氧，在好氧培养过程中，空气是滤过开放的，所以通过摇到可以让更多空气中氧气溶解于发酵液中。厌氧则不是这个作用了。 3.体系均一，有便于对不同参数的取样测定。。
