| FLLL32STAT3 inhibitor |

Sample solution is provided at 25 µL, 10mM.
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Cell Stem Cell.2017 Nov 20. pii: S1934-5909(17)30375-2.Quality Control & MSDS
- View current batch:
- Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
- Datasheet
Chemical structure


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| Cas No. | 1226895-15-3 | SDF | Download SDF |
| Chemical Name | (2E,2"E)-1,1"-(cyclohexane-1,1-diyl)bis(3-(3,4-dimethoxyphenyl)prop-2-en-1-one) | ||
| Canonical SMILES | COC1=C(OC)C=C(/C([H])=C([H])/C(C2(C(/C([H])=C([H])/C3=CC(OC)=C(OC)C=C3)=O)CCCCC2)=O)C=C1 | ||
| Formula | C28H32O6 | M.Wt | 464.55 |
| Solubility | Soluble in DMSO | Storage | Store at -20°C |
| Physical Appearance | A crystalline solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
FLLL32 is a small molecule analog of curcumin. It can inhibit signal transducer and activator of transcription-3 (STAT3). In the pSTAT3-positive A375 cell line, FLLL32 resulted in pro-apoptotic effects with an IC50 value of 1.3 μM at 48 hours [1].
Activation of STAT3 in melanoma tumors is related to poor prognosis. STAT3 can promote angiogenesis and cell proliferation, drive invasion and metastasis, and inhibit apoptosis [1].
In multiple human melanoma cell lines, after a 24 hour treatment with FLLL32, the STAT3 phosphorylation at Tyr705 but not at Tyr727 was inhibited. In a prior in vitro cell-free assay, FLLL32 was found to inhibit the activity of Jak2 kinase. But in the experiment with multiple human melanoma cell line, no appreciable alteration in the phosphorylation of Jak2, even when the concentration of FLLL32 was 8 μM, was observed. This meant that FLLL32 likely directly acted against the STAT3 protein. FLLL32 at concentrations ranged from 2-4 μM for only 4 hours reduced pSTAT3 and induce cell death [1].
In an s.c. model of B16F10 melanoma in C57BL/6 mice, compared to the treatment with DMSO to the same animals, daily i.p. administration of FLLL-32 at concentrations of 25 mg/kg and 50 mg/kg significantly delayed tumor growth (p<0.001)>0.001)>
References: [1]. Bill MA, Fuchs JR, Li C, et al. The small molecule curcumin analog FLLL32 induces apoptosis in melanoma cells via STAT3 inhibition and retains the cellular response to cytokines with anti-tumor activity. Molecular cancer, 2010, 9(1): 165.[2]. Lesinski G, Bill M, Li C, et al. Abstract# 856: The small molecule curcumin analog FLLL-32 induces apoptosis in human melanoma cells via STAT3 inhibition and elicits in vivo anti-tumor activity. Cancer Research, 2009, 69(9 Supplement): 856-856.
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如题,WB中,脱脂牛奶封闭时,忘记使用脱色摇床摇晃,对封闭效果影响大不大?有没有什么补救办法?延长封闭时间还是怎么办?急等,谢谢!!!!
2.溶氧,在好氧培养过程中,空气是滤过开放的,所以通过摇到可以让更多空气中氧气溶解于发酵液中。厌氧则不是这个作用了。 3.体系均一,有便于对不同参数的取样测定。。
水浴恒温摇床可分为常温水浴恒温摇床(室温~100℃)和冷冻水浴恒温摇床(常用为0~100℃,也可定制更低温度的如:-10℃~100℃)。在运行方式上可分为往复式、回旋式和双功能水浴恒温摇床。气浴恒温摇床也可分为常温恒温摇床和冷冻恒温摇床。常温气浴恒温摇床的温度范围为:室温+5~60℃。冷冻气浴恒温摇床的温度范围为:4~60℃。在运行方式上也分为往复式、回旋式和双功能气浴恒温摇床。你可以根据实验的具体要求来选择合适的摇床。向左转|向右转向左转|向右转向左转|向右转
1.传质。就是底物或代谢产物更好在体系内转移和发挥作用。
2.溶氧。在好氧培养过程中,空气是滤过开放的,所以通过摇到可以让更多空气中氧气溶解于发酵液中。
3.体系均一。有便于对不同参数的取样测定。
ZD-85双功能气浴恒温摇床型号:
(1)CHA-S型、恒温气浴摇床:属于-往复式
(2)SHZ-82型、恒温气浴摇床:属于-回旋式
(3)ZD-85型、恒温气浴摇床:属于-往复和回旋式、双功能。
ZD-85双功能气浴恒温摇床主要技术参数:
1、 使用电源: 220V 50Hz
2、 加热功率: 400w
3、 定时范围: 0~120分(或常开)
4、 振荡频率: 起动—300转/分,可调
5、 振荡幅度: 20mm
6、 恒温范围: 室温—50℃
7、 振荡方法: 往复、回旋和双功能(采购时选择)
8: 温控精度: +0.5℃
9: 装瓶量:试管:16×300mm 100ml×24只、200ml×15只
10: 外形尺寸: 700×470×500mm
THZ-82水浴恒温摇床技术指标
温控均匀性:≤±0.5℃
温控范围:室温-100 ℃
温度波动:±0.5℃
加热功率:1800W
振荡频率:起动-280次/分
振幅:20mm
定时范围:0-120分(或常开)
工作电源:AC220V 50Hz
水浴恒温摇床型号与振荡方式
SHA-C型为往复水浴恒温摇床
THZ-82型为回旋水浴恒温摇床
SHA-B型为双功能水浴恒温摇床
大容量恒温摇床参数 产品名称
双层恒温摇床
产品型号TS-1112B
显示方式液晶一屏显示
电源电压AC220V 50HZ
控温范围室温+5~60℃
温度分辨率0.1℃
照明方式内置照明灯
制冷方式自动
压缩机化霜方式自动
波动度±0.1℃
旋转频率30-280rpm
转速精度±1rpm
摆振幅度0-50mm无极可调
标准配置250ml*45支 500ml*36支
最大容量250ml*90支或50ml*72支或1000ml*36支
托盘尺寸970*560mm
震荡方式回旋式
工作环境温度5℃~40℃
输入功率750W
内胆尺寸(W*D*H)mm1120*640*830mm(595L)
外形尺寸(W*D*H)mm1200*800*150mm
拖盘数量2块
净重360KG
定时范围0~999小时
参考资料:Hi.baidu/=。www.jingda17.net。DI展开

