![SMOBIO/[QP3310] Q-PAGE™ Bis-Tris Precast Gel (Midi, 12 wells, 12%), 10 gels/Midi, 12 wells, 12%), 10 gels</span>
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Description
Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Tris-Glycine gels.
Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.
Key Features
User-friendly gel cassette:
Numbered and framed wells for sample loading
With cassette opener for easy use
Enhanced gel performance:
Enhanced band sharpness
Better resolution of small proteins
Stable for shipping at ambient temperature
Easy compatibility:
Available as homogeneous and adjusted gradient gels for a wide range of protein separation.
Compatible with most popular protein electrophoresis systems
Storage and stability
Store Q-PAGE™ Precast Gels at 4°C for periods up to 12 months.
Do not freeze Q-PAGE™ Precast Gels Remove tape and comb before electrophoresis.
Technical
Clear and sharp bands, high resolution
Q-PAGE™ Bis-Tris Precast Gel shows high resolution of protein separation.
QP3310 Specifications
Gel | Bis-Tris | |
Buffer systems | MOPS and MES | |
Features | Clear and sharp bands, high resolution | |
Cassette size | Midi Gel (10 X 10 cm) | |
Gel dimensions | 8.1 x 8.1 x 0.1 cm (W x L x thickness) cm | |
Electrophoresis system | Mini Gel Tank XCell SureLock, Hoefer SE260 | |
Well format & Capacity | 12 wells, 40 μl/well | |
Gel percentage | 12 % | |
Accessory tray | Production description Tip card Gel remover Cassette opener | |
Manual
Manual_Q-PAGE™ Bis-Tris Precast Gel, Midi
SDS
SDS_Q-PAGE™ Precast Gel
Migration pattern
Setting Up and Running Q-PAGE™ Midi Precast Gel
Removing Q-PAGE from cassette
Setting up gel/membrane sandwich for Western transfer
Recommendations/Tips for Gel Running
1. Remove comb and tape before adaption. 2. Use fresh 1X running buffer for the inner cathode chamber. 3. Do not use Tris-Glycine running buffer for Q-PAGE™ Bis-Tris Precast Gels. 4. Rinse the wells before sample loading.
Sample Preparation for SDS-PAGE
1. Mix protein sample with 2X sample buffer.
2. Heat the diluted samples at 95°C for 5 min or at 70°C for 10 min.
3. Cool the diluted samples to 4°C and spin down the water condensed on tube surface. (If there is high viscosity part at bottom of tube, transfer supernatant to a new tube.)
Prepare Q-PAGE™ for Sample Loading
1.Open the blister tray of Q-PAGE™ Precast Gel.
2.Briefly rinse the gel cassette with ddH2O.
3.Remove tape and comb; avoid squeezing the gel.
4.Adapt Q-PAGE™ to electrophoresis system; instruction is provided below. (Invitrogen® Mini Gel Tank is recommended.)
5.Use a pipette to gently wash the wells with running buffer to remove residual storage buffer.
6.Fill the wells with running buffer prior to sample loading.
7.Load samples and pre-stained protein marker into numbered wells.
8.Fill both inner and outer chambers with running buffer to the highest level. Ensure gel wells are completely covered.
Power Setting for Running Q-PAGE™
Optimize the voltage and running time if needed.
| 130 V | 180 V | 230 V*2 |
Running Time*1 | 60-75 mins | 35-50 mins | 25-40 mins |
Expected Current Initial (per gel) Final (per gel) |
70-80 mA 20-30 mA |
90-100 mA 35-45 mA |
130-140 mA 60-70 mA |
Expected temperature | 25-30°C | 25-35 °C | 35-45°C |
*1 Set voltage higher than 100 V is recommended.
*2 For higher voltage conditions, please use fresh running buffer for inner and outer chambers.
*3 Running time varies depending on gel percentage, running buffer, temperature, and power supply.
Remove Q-PAGE™ Midi Gel from Cassette
Open cassette immediately after electrophoresis. Avoid gel drying.
1.Insert the cassette opener into corners of cassette.
2.Sequentially pry the opener to separate the two plates.
3.Gently pull up notched plate and let gel stay on the front plate.
4.Use cassette opener to push through the slot in the cassette.
5.Carefully detach the gel from the bottom of gel
- Avoid diagonally peeling the gel from the corner.
- If necessary, cut well separators with gel remover
6.Gently remove the gel for further staining or Western blotting.
Gel Staining
Proteins separated using Q-PAGE™ Precast Gels can be further stained with most popular staining reagents, such as Coomassie dyes (R-250 or G-250), Silver-stain solution,
and FluoroStain™ Protein Fluorescent Staining Dye. (Cat. No. PS1000)
Transferring Protein from Q-PAGE™ to Blotting Membrane
1. After protein separation using Q-PAGE™, gently detach QPAGE™ from cassette and then equilibrate the gel in transfer buffer.
2. Pre-soak blotting membrane and filter papers in transfer buffer.
*Activate PVDF membrane in methanol before soaking in transfer buffer.
**Prepare 6 filter papers for one gel/membrane sandwich.
3. Assemble transfer sandwich by orientating cathode, sponge, filter papers, gel, membrane, filter papers, sponge, and anode. The protein goes to the direction of cathode to anode.
4. Carefully move roller over the gel/membrane to remove air bubbles and excess buffer until complete contact is established.
5. Insert transfer cassette into transfer module. Notice that black side of cassette should be next to black side of module.
6. Fill transfer tank with pre-cooled transfer buffer to the highest water level.
7. Set constant voltage at 100 V. Transfer for 90 minutes at low temperature condition. Pre-stained protein marker should be visible on the membrane after transfer is completed.
Transfer of proteins to the membrane can be checked using Ponceau S staining before blocking step.
Supplemental Information for Using Q-PAGE™ Precast Gel
Adapting Q-PAGE™ Midi Precast Gels to Invitrogen Mini Gel Tank Electrophoresis System
1. Place the Q-PAGE Midi Precast Gels with notched plate facing toward yourself. No extra adapter is needed.
2. Seat the gels on the bottom of Mini Gel Tank and close the cassette clamp.
3. Fill chambers with running buffer to the level of the fill line. Ensure gel wells are completely covered.
Adapting Q-PAGE™ Midi Precast Gels to other electrophoresis system, please follow the manufacturer’s instruction.
Buffer recipes
2X sample buffer with reducing agent
62.5 mM Tris-HCl pH 6.8, 2% SDS, 25% (v/v) glycerol, 0.01% bromophenol blue, 5% β-mercaptoethanol or 100 mM DTT (added fresh)
10X MOPS running buffer
60.6 g Tris base, 104.6 g MOPS, 10.0 g SDS, 3.0 g EDTA. Bring up the volume to 1 L with ddH2O.
10X MES running buffer
60.6 g Tris base, 97.6 g MES, 10.0 g SDS, 3.0 g EDTA. Bring up the volume to 1 L with ddH2O.
1X running buffer
Dilute 100 ml 10X running buffer with 900 ml ddH2O.
10X transfer buffer
30.0 g Tris base, 144.0 g Glycine. Bring up the volume to 1 L with ddH2O.
1X transfer buffer
*Cool 1X transfer buffer to 4°C before using.
Dilute 100 ml 10X transfer buffer with 200 ml methanol and 700 ml ddH2O.
**Add SDS to 0.1% to promote transfer of high molecular weight proteins.
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Q-PAGE™ Precast Gel
Gel Type | Bis-Tris | TGN (Tris-Glycine-Novel) | ||||||
Buffer systems | MOPS and MES | Tris-Glycine (Laemmli) | ||||||
Features | Clear and sharp bands, high resolution | Quick running, clear bands | ||||||
Cassette size | Mini Gel(10 x 8.3 cm) | Midi Gel(10 X 10 cm) | Mini Gel(10 x 8.3 cm) | Midi Gel(10 X 10 cm) | ||||
Electrophoresis system | Bio-Rad systems | Mini Gel Tank Xcell SureLock, Hoefer SE260 | Bio-Rad systems | Mini Gel Tank Xcell SureLock, Hoefer SE260 | ||||
Well format & Capacity | 12 wells, 25 μl/well | 15 wells, 22 μl/well | 12 wells, 40 μl/well | 15 wells, 28 μl/well | 12 wells, 25 μl/well | 15 wells, 22 μl/well | 12 wells, 40 μl/well | 15 wells, 28 μl/well |
Gel percentage/ Cat. No. | 8% | 8% | 8% | 8% | 10% | 10% | 10% | 10% |
QP2110 | QP2120 | QP3110 | QP3120 | QP4210 | QP4220 | QP5210 | QP5220 | |
12% | 12% | 12% | 12% | 4-15% | 4-15% | 4-15% | 4-15% | |
QP2310 | QP2320 | QP3310 | QP3320 | QP4510 | QP4520 | QP5510 | QP5520 | |
4-12% | 4-12% | 4-12% | 4-12% |
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QP2510 | QP2520 | QP3510 | QP3520 |
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ExcelBand™ Protein Markers
Ready-to-use— premixed with a loading buffer for direct loading, no need to boil
Broad range— 310 kDa to 5 kDa
Pre-stained bands — for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane
Enhanced bands— for quick reference
YesBlot™ Western Marker I
Ready-to-use — no need of mixing or heating before sample loading
Direct visualization — 10 IgG-binding proteins for direct visualization on Western blots
Pre-stained bands — 4 pre-stained proteins for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane
Wide range — 10 clear bands from 15 to 200 kDa for size estimation
Quick reference — two enhanced bands (30 and 80 kDa)
FluoroStain™ Protein Fluorescent Staining Dye
Compatible to MASS analysis — compatible to the analysis of mass spectra, such as LC-MS/MS, MALDI-TOF, and etc.
High sensitivity — detection level achieve ~3 ng, similar to silver staining
Substitution of the Coomassie Blue protein staining method
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如题，WB中，脱脂牛奶封闭时，忘记使用脱色摇床摇晃，对封闭效果影响大不大？有没有什么补救办法？延长封闭时间还是怎么办？急等，谢谢！！！！
2.溶氧，在好氧培养过程中，空气是滤过开放的，所以通过摇到可以让更多空气中氧气溶解于发酵液中。厌氧则不是这个作用了。 3.体系均一，有便于对不同参数的取样测定。。
水浴恒温摇床可分为常温水浴恒温摇床（室温~100℃）和冷冻水浴恒温摇床（常用为0~100℃，也可定制更低温度的如：-10℃~100℃）。在运行方式上可分为往复式、回旋式和双功能水浴恒温摇床。气浴恒温摇床也可分为常温恒温摇床和冷冻恒温摇床。常温气浴恒温摇床的温度范围为：室温+5~60℃。冷冻气浴恒温摇床的温度范围为：4~60℃。在运行方式上也分为往复式、回旋式和双功能气浴恒温摇床。你可以根据实验的具体要求来选择合适的摇床。向左转|向右转向左转|向右转向左转|向右转
1.传质。就是底物或代谢产物更好在体系内转移和发挥作用。
2.溶氧。在好氧培养过程中，空气是滤过开放的，所以通过摇到可以让更多空气中氧气溶解于发酵液中。
3.体系均一。有便于对不同参数的取样测定。
ZD-85双功能气浴恒温摇床型号：
（1）CHA－S型、恒温气浴摇床：属于－往复式
（2）SHZ-82型、恒温气浴摇床：属于－回旋式
（3）ZD－85型、恒温气浴摇床：属于－往复和回旋式、双功能。
ZD-85双功能气浴恒温摇床主要技术参数：
1、 使用电源： 220V 50Hz
2、 加热功率： 400w
3、 定时范围： 0~120分（或常开）
4、 振荡频率： 起动—300转/分,可调
5、 振荡幅度： 20mm
6、 恒温范围： 室温—50℃
7、 振荡方法： 往复、回旋和双功能（采购时选择）
8： 温控精度： +0.5℃
9： 装瓶量：试管：16×300mm 100ml×24只、200ml×15只
10： 外形尺寸： 700×470×500mm
THZ-82水浴恒温摇床技术指标
温控均匀性：≤±0.5℃
温控范围：室温-100 ℃
温度波动：±0.5℃
加热功率：1800W
振荡频率：起动-280次/分
振幅：20mm
定时范围：0-120分(或常开)
工作电源：AC220V 50Hz
水浴恒温摇床型号与振荡方式
SHA-C型为往复水浴恒温摇床
THZ-82型为回旋水浴恒温摇床
SHA-B型为双功能水浴恒温摇床
大容量恒温摇床参数 产品名称
双层恒温摇床
产品型号TS-1112B
显示方式液晶一屏显示
电源电压AC220V 50HZ
控温范围室温+5～60℃
温度分辨率0.1℃
照明方式内置照明灯
制冷方式自动
压缩机化霜方式自动
波动度±0.1℃
旋转频率30-280rpm
转速精度±1rpm
摆振幅度0-50mm无极可调
标准配置250ml*45支 500ml*36支
最大容量250ml*90支或50ml*72支或1000ml*36支
托盘尺寸970*560mm
震荡方式回旋式
工作环境温度5℃～40℃
输入功率750W
内胆尺寸（W*D*H）mm1120*640*830mm（595L）
外形尺寸（W*D*H）mm1200*800*150mm
拖盘数量2块
净重360KG
定时范围0～999小时
参考资料:Hi.baidu/=。www.jingda17.net。DI展开
