Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
---|---|---|---|---|---|---|
Plasmid | 101061 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | Add to Cart | |
AAV1 | 101061-AAV1 | Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information | Add to Cart |
This material is available to academics and nonprofits only.
Backbone
- Vector backbonepAAV(Search Vector Database)
- Backbone sizew/o insert(bp)4267
- Total vector size (bp)5716
- Vector typeAAV
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)NEB Stable
- Copy numberHigh Copy
Gene/Insert
- Gene/Insert nameCaMPARI2-F391W
- SpeciesSynthetic
- Insert Size (bp)1446
- Promoterhsyn (synapsin-1)
- Tags/ Fusion Proteins
- NES_his (N terminal on insert)
- FLAG-HA-myc (C terminal on insert)
Cloning Information
- Cloning methodGibson Cloning
- 5′ sequencing primerf.hsyn
- 3′ sequencing primerr.WPRE (Common Sequencing Primers)
Resource Information
- Supplemental Documents
- pAAV_hsyn1_NES-his-CaMPARI2-F391W-WPRE-SV40.gb
- Terms and Licenses
- UBMTA
- Industry Terms
- Not Available to Industry
Depositor Comments
Point mutant (F391W) of CaMPARI2 (Addgene #101060). Kd = 110nM
Information for AAV1 (Catalog # 101061-AAV1)(Back to top)
Purpose
Ready-to-use AAV1 particles produced from pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40 (#101061). In addition to the viral particles, you will also receive purified pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40 plasmid DNA.
Synapsin-driven, photoconvertible (irreversible green-to-red) calcium integrator his-tagged CaMPARI2-F391W. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 1×10¹³ vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV1
- PurificationIodixanol gradient ultracentrifugation
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
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ZD-85双功能气浴恒温摇床型号:
(1)CHA-S型、恒温气浴摇床:属于-往复式
(2)SHZ-82型、恒温气浴摇床:属于-回旋式
(3)ZD-85型、恒温气浴摇床:属于-往复和回旋式、双功能。
ZD-85双功能气浴恒温摇床主要技术参数:
1、 使用电源: 220V 50Hz
2、 加热功率: 400w
3、 定时范围: 0~120分(或常开)
4、 振荡频率: 起动—300转/分,可调
5、 振荡幅度: 20mm
6、 恒温范围: 室温—50℃
7、 振荡方法: 往复、回旋和双功能(采购时选择)
8: 温控精度: +0.5℃
9: 装瓶量:试管:16×300mm 100ml×24只、200ml×15只
10: 外形尺寸: 700×470×500mm
THZ-82水浴恒温摇床技术指标
温控均匀性:≤±0.5℃
温控范围:室温-100 ℃
温度波动:±0.5℃
加热功率:1800W
振荡频率:起动-280次/分
振幅:20mm
定时范围:0-120分(或常开)
工作电源:AC220V 50Hz
水浴恒温摇床型号与振荡方式
SHA-C型为往复水浴恒温摇床
THZ-82型为回旋水浴恒温摇床
SHA-B型为双功能水浴恒温摇床
大容量恒温摇床参数 产品名称
双层恒温摇床
产品型号TS-1112B
显示方式液晶一屏显示
电源电压AC220V 50HZ
控温范围室温+5~60℃
温度分辨率0.1℃
照明方式内置照明灯
制冷方式自动
压缩机化霜方式自动
波动度±0.1℃
旋转频率30-280rpm
转速精度±1rpm
摆振幅度0-50mm无极可调
标准配置250ml*45支 500ml*36支
最大容量250ml*90支或50ml*72支或1000ml*36支
托盘尺寸970*560mm
震荡方式回旋式
工作环境温度5℃~40℃
输入功率750W
内胆尺寸(W*D*H)mm1120*640*830mm(595L)
外形尺寸(W*D*H)mm1200*800*150mm
拖盘数量2块
净重360KG
定时范围0~999小时
参考资料:Hi.baidu/=。www.jingda17.net。DI展开
培养皿上的细菌是一个月前接种的,培养基成分没有变化