
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
---|---|---|---|---|---|---|
Plasmid | 101061 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | Add to Cart | |
AAV1 | 101061-AAV1 | Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information | Add to Cart |
This material is available to academics and nonprofits only.
Backbone
- Vector backbonepAAV(Search Vector Database)
- Backbone sizew/o insert(bp)4267
- Total vector size (bp)5716
- Vector typeAAV
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)NEB Stable
- Copy numberHigh Copy
Gene/Insert
- Gene/Insert nameCaMPARI2-F391W
- SpeciesSynthetic
- Insert Size (bp)1446
- Promoterhsyn (synapsin-1)
- Tags/ Fusion Proteins
- NES_his (N terminal on insert)
- FLAG-HA-myc (C terminal on insert)
Cloning Information
- Cloning methodGibson Cloning
- 5′ sequencing primerf.hsyn
- 3′ sequencing primerr.WPRE (Common Sequencing Primers)
Resource Information
- Supplemental Documents
- pAAV_hsyn1_NES-his-CaMPARI2-F391W-WPRE-SV40.gb
- Terms and Licenses
- UBMTA
- Industry Terms
- Not Available to Industry
Depositor Comments
Point mutant (F391W) of CaMPARI2 (Addgene #101060). Kd = 110nM
Information for AAV1 (Catalog # 101061-AAV1)(Back to top)
Purpose
Ready-to-use AAV1 particles produced from pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40 (#101061). In addition to the viral particles, you will also receive purified pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40 plasmid DNA.
Synapsin-driven, photoconvertible (irreversible green-to-red) calcium integrator his-tagged CaMPARI2-F391W. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 1×10¹³ vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV1
- PurificationIodixanol gradient ultracentrifugation
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
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培养皿上的细菌是一个月前接种的,培养基成分没有变化
如题,WB中,脱脂牛奶封闭时,忘记使用脱色摇床摇晃,对封闭效果影响大不大?有没有什么补救办法?延长封闭时间还是怎么办?急等,谢谢!!!!
1.传质。就是底物或代谢产物更好在体系内转移和发挥作用。
2.溶氧。在好氧培养过程中,空气是滤过开放的,所以通过摇到可以让更多空气中氧气溶解于发酵液中。
3.体系均一。有便于对不同参数的取样测定。

